Quantifying cilia beat frequency using high‐speed video microscopy: Assessing frame rate requirements when imaging different ciliated tissues DOI Creative Commons

Luke Scopulovic,

Deanne Francis, Elvis Pandžić

et al.

Physiological Reports, Journal Year: 2022, Volume and Issue: 10(11)

Published: June 1, 2022

Motile cilia are found in numerous locations throughout our body and play a critical role various physiological processes. The most commonly used method to assess motility is quantify beat frequency (CBF) via video microscopy. However, large heterogeneity exists within published literature regarding the framerate image for calculating CBF. aim of this study was determine optimal frame rate required CBF assessment, if Nyquist theorem may be set rate. One-second movies were collected at >600 fps from mouse airways ependyma room-temperature or 37°C. Movies then down-sampled 30-300 fps. quantified identical different framerates by either manual counting automated MATLAB script. Airway significantly impaired 30 movies, while ependymal both 60 movies. Pairwise comparison showed that should least 150 accurately measure CBF, with minimal improvement accuracy >150 script also less accurate measuring lower than counting, however, difference disappeared higher (>150 fps). In conclusion, data suggest unreliable setting sampling measurement. Instead, 3-4 times faster assessment. Especially calculation automated.

Language: Английский

An alternative cell cycle coordinates multiciliated cell differentiation DOI
Semil P. Choksi, Lauren Byrnes, Mia J. Konjikusic

et al.

Nature, Journal Year: 2024, Volume and Issue: 630(8015), P. 214 - 221

Published: May 29, 2024

Language: Английский

Citations

19
Motile cilia modulate neuronal and astroglial activity in the zebrafish larval brain DOI Creative Commons
Percival P. D’Gama, Inyoung Jeong, Andreas Moe Nygård

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(1), P. 115195 - 115195

Published: Jan. 1, 2025

Language: Английский

Citations

2
PTEN mutations impair CSF dynamics and cortical networks by dysregulating periventricular neural progenitors DOI
Tyrone DeSpenza,

Emre Kiziltug,

Garrett Allington

et al.

Nature Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Language: Английский

Citations

2
Mechanisms of neuroinflammation in hydrocephalus after intraventricular hemorrhage: a review DOI Creative Commons
Katherine G. Holste, Fan Xia,

Fenghui Ye

et al.

Fluids and Barriers of the CNS, Journal Year: 2022, Volume and Issue: 19(1)

Published: April 1, 2022

Intraventricular hemorrhage (IVH) is a significant cause of morbidity and mortality in both neonatal adult populations. IVH not only causes immediate damage to surrounding structures by way mass effect elevated intracranial pressure; the subsequent inflammation additional brain injury edema. Of those neonates who experience severe IVH, 25-30% will go on develop post-hemorrhagic hydrocephalus (PHH). PHH places adults at risk for white matter injury, seizures, death. Unfortunately, molecular determinants are well understood. Within past decade an emphasis has been placed neuroinflammation PHH. More information come light regarding inflammation-induced fibrosis cerebrospinal fluid hypersecretion response IVH. The aim this review discuss role involving clot-derived neuroinflammatory factors including hemoglobin/iron, peroxiredoxin-2 thrombin, as macrophages/microglia, cytokines complement development Understanding mechanisms after may highlight potential novel therapeutic targets

Language: Английский

Citations

50
Primary Ciliary Dyskinesia: A Clinical Review DOI Creative Commons
Katherine A. Despotes, Maimoona A. Zariwala, Stephanie D. Davis

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(11), P. 974 - 974

Published: June 4, 2024

Primary ciliary dyskinesia (PCD) is a rare, genetically heterogeneous, motile ciliopathy, characterized by neonatal respiratory distress, recurrent upper and lower tract infections, subfertility, laterality defects. Diagnosis relies on combination of tests for confirmation, including nasal nitric oxide (nNO) measurements, high-speed videomicroscopy analysis (HSVMA), immunofluorescent staining, axonemal ultrastructure via transmission electron microscopy (TEM), genetic testing. Notably, there no single gold standard confirmatory or exclusionary test. Currently, 54 causative genes involved in cilia assembly, structure, function have been linked to PCD; this rare disease has spectrum clinical manifestations emerging genotype–phenotype relationships. In review, we provide an overview the structure cilia, genetics pathophysiology disease, as well features associated with ciliopathies, novel diagnostic tools, updates relationships PCD.

Language: Английский

Citations

13
Rethinking the cilia hypothesis of hydrocephalus DOI
Phan Q. Duy, Ana B.W. Greenberg, William E. Butler

et al.

Neurobiology of Disease, Journal Year: 2022, Volume and Issue: 175, P. 105913 - 105913

Published: Oct. 30, 2022

Language: Английский

Citations

32
Ependymal Cilia: Physiology and Role in Hydrocephalus DOI Creative Commons

Weiye Ji,

Zhi Tang, Yibing Chen

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2022, Volume and Issue: 15

Published: July 12, 2022

Cerebrospinal fluid (CSF), a colorless liquid that generally circulates from the lateral ventricles to third and fourth ventricles, provides essential nutrients for brain homeostasis growth factors during development. As evidenced by an increasing corpus of research, CSF serves range important functions. While it is considered decreased flow associated development hydrocephalus, has recently been postulated motile cilia, which line apical surfaces ependymal cells (ECs), play role in stimulating circulation cilia beating. Ependymal protrude ECs, their synchronous pulsing transports ventricle then subarachnoid cavity absorption. result, we malfunctioning could disrupt normal flow, raising risk hydrocephalus. This review aims demonstrate physiological functions as well how immobility or disorientation causes problems. We also conclude conceivable ways treatment hydrocephalus currently clinical application provide theoretical support regimen improvements investigating relationship between

Language: Английский

Citations

30
Essential Roles of Efferent Duct Multicilia in Male Fertility DOI Creative Commons
Mohammed Hoque, Eunice N. Kim, Danny Chen

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(3), P. 341 - 341

Published: Jan. 20, 2022

Cilia are microtubule-based hair-like organelles on the cell surface. have been implicated in various biological processes ranging from mechanosensation to fluid movement. Ciliary dysfunction leads a plethora of human diseases, known as ciliopathies. Although non-motile primary cilia ubiquitous, motile multicilia found restricted locations body, such respiratory tract, oviduct, efferent duct, and brain ventricles. Multicilia beat whip-like motion generate flow over apical surface an epithelium. The concerted ciliary provides driving force critical for clearing airway mucus debris, transporting ova ovary uterus, maintaining sperm suspension, circulating cerebrospinal brain. In male reproductive multiciliated cells (MCCs) were first described mid-1800s, but their importance fertility remained elusive until recently. MCCs exist ducts, which small, highly convoluted tubules that connect testis epididymis play essential role fertility. this review, we will introduce multiciliogenesis, discuss mouse models infertility with defective multicilia, summarize our current knowledge function tract.

Language: Английский

Citations

23
Ependymal cells and neurodegenerative disease: outcomes of compromised ependymal barrier function DOI Creative Commons
Diana G. Nelles, Lili‐Naz Hazrati

Brain Communications, Journal Year: 2022, Volume and Issue: 4(6)

Published: Nov. 1, 2022

Within the central nervous system, ependymal cells form critical components of blood-cerebrospinal fluid barrier and cerebrospinal fluid-brain barrier. These barriers provide biochemical, immunological physical protection against entry molecules foreign substances into while also regulating dynamics, such as composition, flow removal waste from fluid. Previous research has demonstrated that several neurodegenerative diseases, Alzheimer's disease multiple sclerosis, display irregularities in cell function, morphology, gene expression metabolism. Despite playing key roles maintaining overall brain health, are largely overlooked understudied context disease, thus limiting development novel diagnostic treatment options. Therefore, this review explores anatomical properties, functions structures define healthy brain, well ways which dysregulation manifests across diseases. Specifically, we will address potential mechanisms, causes consequences dysfunction describe how compromising integrity may initiate, contribute to, or drive widespread neurodegeneration brain.

Language: Английский

Citations

23
Choroid plexus epithelium and its role in neurological diseases DOI Creative Commons

Ruizhen Liu,

Zhiping Zhang, Yibing Chen

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2022, Volume and Issue: 15

Published: Oct. 21, 2022

Choroid plexus epithelial cells can secrete cerebrospinal fluid into the ventricles, serving as major structural basis of selective barrier between neurological system and blood in brain. In fact, choroid release majority fluid, which is connected with particular ion channels cells. also produce a number essential growth factors peptides that help injured cerebrovascular heal. The pathophysiology neurodegenerative disorders like Alzheimer's disease, Parkinson's well minor brain damage diseases hydrocephalus stroke still unknown. Few studies have previously to etiology these serious disorders. Therefore, hopes discovering novel treatment options for linked conditions, this review extensively analyzes association such disease hydrocephalus. Finally, we CPE based immunotherapy, cauterization, transplantation, gene therapy.

Language: Английский

Citations

21