Clinical and laboratory significance of soluble claudin-5 and VEGF in ovarian cancer DOI Creative Commons
Н. Е. Кушлинский,

S. Kulikova,

Frol A. Gugnin

et al.

Advances in molecular oncology, Journal Year: 2025, Volume and Issue: 12(1), P. 67 - 75

Published: April 15, 2025

Introduction. Claudin 5 is belongs to a family of transmembrane proteins mediating formation tight junctions between cells, maintenance cell polarity in epithelial and endothelial layers, regulation membrane permeability, control signal transduction inside the cells. Results small number studies show that vascular growth factor (VEGF) also affects junctions, particular through claudin expression. In addition their physiological functions, VEGF play an important role pathogenesis various diseases including malignant neoplasms. Aim. To study levels serum patients with ovarian cancer evaluate clinical significance. Materials methods. total, 123 (median age 54 years) 32 group healthy women were examined. measured prior treatment using Human CLDN5 (Claudin 5) ELISA Kit (Elabscience, China) Immunoassay (Quantikine®, R&D Systems, uSA) accordance manufacturer’s instructions. Statistical analysis obtained data was performed GraphPad Prizm v. 10 software. The values compared correlations quantified nonparametric Mann–Whitney, Kruskal–Wallis tests Spearman’s rank correlation coefficient. Overall survival analyzed Kaplan–Meier method. Results. found 97 % 94 women. Median (1st quartile (Q1) – 3rd (Q3)) level 0.77 (0.48–1.17) ng/mL, 0.95 (0.43–1.77) mg/mL. ROC informational value showed unsatisfactory diagnostic accuracy model (area under curve (AuC) 0.613 (95 confidence interval (CI) 0.513–0.713); p = 0.049): for median threshold ng/mL assay had sensitivity 50 specificity 60 %. all women; (Q1–Q3) 45.6 (13.3–89.09) pg/mL statistically significantly lower than cancer: 274.7 (199.0–472.5). good (AuC 0.942 CI 0.886–0.997); <0.0001) which allows use as criterion. best results (71 100 %, respectively) at 226.2 pg/mL. Serum are associated progression. However, not significant prognostic markers this disease. Conclusion. higher positively correlate each other. Additionally, has relatively characteristics group. presence distant metastases points potential tumor research stage, these cannot be recommended or criteria require further study.

Language: Английский

Interrogating mediators of single-cell transcriptional changes in the acute damaged cerebral cortex: Insights into endothelial-astrocyte interactions DOI
Caroline de Jager, Eman Soliman, Michelle H. Theus

et al.

Molecular and Cellular Neuroscience, Journal Year: 2025, Volume and Issue: unknown, P. 104003 - 104003

Published: March 1, 2025

Language: Английский

Citations

0
Rutin ameliorates stress–induced blood‒brain barrier dysfunction and cognitive decline via the endothelial HDAC1‒Claudin–5 axis DOI Creative Commons
Zhaowei Sun,

Zhao-Xin Sun,

Yun Zhao

et al.

Fluids and Barriers of the CNS, Journal Year: 2025, Volume and Issue: 22(1)

Published: April 2, 2025

Emerging evidence suggests that chronic stress compromises blood‒brain barrier (BBB) integrity by disrupting brain microvascular endothelial cells (BMECs), contributing to the development of cognitive impairments. Thus, targeting BBB is expected be a promising treatment strategy. The biological function rutin has been investigated in neurological disorders; however, its regulatory role stress-induced damage and decline underlying mechanisms remain elusive. In unpredictable mild (CUMS) mouse model, fluorescent dye assay behavioral tests, including novel object recognition test Morris water maze, were performed evaluate protective effects on cognition. BMEC also hCMEC/D3 (a human cell line) vitro. Furthermore, molecular which restores endothelium dysfunction explored via RNA-seq, quantitative real-time PCR, western blotting, immunofluorescence chromatin immunoprecipitation. Finally, biotinylated tumor necrosis factor-α (TNF-α) was employed influence ability circulating TNF-α cross BBB. We identified attenuated hyperpermeability impairment caused 8-week CUMS procedure. Moreover, promoted proliferation, migration angiogenesis BMECs, cellular monolayer through positively regulating expression genes involved. impeded histone deacetylase 1 (HDAC1) recruitment stabilized H3K27ac increase Claudin-5 protein levels. Ultimately, normalization hippocampal HDAC1‒Claudin-5 axis blocked infiltration into parenchyma alleviated neuroinflammation. This work establishes integrity, reveals potential drug candidate for curing deficits.

Language: Английский

Citations

0
Effect of heat stress on ileal epithelial barrier integrity in broilers divergently selected for high- and low-water efficiency DOI Creative Commons

Elizabeth S. Greene,

Brooklee Roach,

Myriam Cuadrado

et al.

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: April 7, 2025

Water scarcity and rising global temperatures are two of the greatest current future threats to poultry sustainability. Therefore, selection for water efficiency (WE) heat resilience vital importance. Additionally, intestinal integrity is critical importance under challenging conditions maintain nutrient absorption therefore, growth performance broilers. Here, we examined effect chronic cyclic stress (HS) on ileal expression profile tight-junction, gap-junction, adherens, desmosome genes in fourth generation divergently selected low (LWE)- high efficient (HWE)-chicken lines. LWE birds exhibited higher levels gut permeability, regardless temperature, as measured by fluorescein isothiocyanate-dextran (FITC-D). Among claudins (CLDN), Cldn1 showed greater HWE compared LWE, temperature. Cldn5, -16, -20, -34 were all lower during HS. Conversely, Cldn25 was decreased but increased Cldn4 line Cingulin (Cgn) gene HS thermoneutral (TN) condition. Gap junction protein α1 (Gja1) desmoglein 4 (Dsg4) LWE. Cadherin 1 (Cdh1) TN lowest HS, whereas catenin α2 (Ctnna2) desmocollin (Dsc1) highest other groups. This differential key associated with barrier likely contributes phenotype response these

Language: Английский

Citations

0
Construction and Functional Evaluation of 2D and 3D Human Blood–Brain Barrier Models DOI
Toshiki Kurosawa, Yoshiyuki Kubo, Yoshiharu Deguchi

et al.

Current human cell research and applications, Journal Year: 2025, Volume and Issue: unknown, P. 233 - 248

Published: Jan. 1, 2025

Language: Английский

Citations

0
Clinical and laboratory significance of soluble claudin-5 and VEGF in ovarian cancer DOI Creative Commons
Н. Е. Кушлинский,

S. Kulikova,

Frol A. Gugnin

et al.

Advances in molecular oncology, Journal Year: 2025, Volume and Issue: 12(1), P. 67 - 75

Published: April 15, 2025

Introduction. Claudin 5 is belongs to a family of transmembrane proteins mediating formation tight junctions between cells, maintenance cell polarity in epithelial and endothelial layers, regulation membrane permeability, control signal transduction inside the cells. Results small number studies show that vascular growth factor (VEGF) also affects junctions, particular through claudin expression. In addition their physiological functions, VEGF play an important role pathogenesis various diseases including malignant neoplasms. Aim. To study levels serum patients with ovarian cancer evaluate clinical significance. Materials methods. total, 123 (median age 54 years) 32 group healthy women were examined. measured prior treatment using Human CLDN5 (Claudin 5) ELISA Kit (Elabscience, China) Immunoassay (Quantikine®, R&D Systems, uSA) accordance manufacturer’s instructions. Statistical analysis obtained data was performed GraphPad Prizm v. 10 software. The values compared correlations quantified nonparametric Mann–Whitney, Kruskal–Wallis tests Spearman’s rank correlation coefficient. Overall survival analyzed Kaplan–Meier method. Results. found 97 % 94 women. Median (1st quartile (Q1) – 3rd (Q3)) level 0.77 (0.48–1.17) ng/mL, 0.95 (0.43–1.77) mg/mL. ROC informational value showed unsatisfactory diagnostic accuracy model (area under curve (AuC) 0.613 (95 confidence interval (CI) 0.513–0.713); p = 0.049): for median threshold ng/mL assay had sensitivity 50 specificity 60 %. all women; (Q1–Q3) 45.6 (13.3–89.09) pg/mL statistically significantly lower than cancer: 274.7 (199.0–472.5). good (AuC 0.942 CI 0.886–0.997); <0.0001) which allows use as criterion. best results (71 100 %, respectively) at 226.2 pg/mL. Serum are associated progression. However, not significant prognostic markers this disease. Conclusion. higher positively correlate each other. Additionally, has relatively characteristics group. presence distant metastases points potential tumor research stage, these cannot be recommended or criteria require further study.

Language: Английский

Citations

0