Ginger-Derived Exosome-Like Nanoparticles Loaded With Indocyanine Green Enhances Phototherapy Efficacy for Breast Cancer

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 1147 - 1169

Published: Jan. 1, 2025

Phototherapy has remarkable advantages in cancer treatment, owing to its high efficiency and minimal invasiveness. Indocyanine green (ICG) plays an important role photo-mediated therapy. However, it several disadvantages such as poor stability aqueous solutions, easy aggregation of molecules, short plasma half-life. This study aimed develop efficient nanoplatform enhance the effects We developed a novel bio-nanoplatform by integrating edible ginger-derived exosome-like nanoparticles (GDNPs) photosensitizer, ICG (GDNPs@ICG). GDNPs were isolated from ginger juice loaded with co-incubation. The size distribution, zeta potential, morphology, total lipid content, drug release behavior GDNPs@ICG characterized. photothermal performance, cellular uptake cytotoxicity, anti-tumor effects, mechanism action investigated both vitro vivo. taken up tumor cells via lipid-dependent pathway. When irradiated 808 nm NIR laser, generated levels ROS, MDA, local hyperthermia within tumor, which caused peroxidation ER stress, thus enhancing breast therapy effect. Furthermore, vivo studies demonstrated that engineered significantly inhibited growth presented limited toxicity. Moreover, detecting expression CD31, N-cadherin, IL-6, IFN-γ, CD8, p16, p21, p53 tissues, we found substantially reduced angiogenesis, metastasis, activated immune response, promoted cell senescence tumor. Our enhanced therapeutic effect could be alternative for precise phototherapy.

Language: Английский

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Green Synthesis and Characterization of Ginger-Derived Silver Nanoparticles and Evaluation of Their Antioxidant, Antibacterial, and Anticancer Activities

Plants, Journal Year: 2024, Volume and Issue: 13(9), P. 1255 - 1255

Published: April 30, 2024

The efficacy, targeting ability, and biocompatibility of plant-based nanoparticles can be exploited in fields such as agriculture medicine. This study highlights the use ginger an effective promising strategy against cancer for treatment prevention bacterial infections related disorders. Ginger is a well-known spice with significant medicinal value due to its phytochemical constituents including gingerols, shogaols, zingerones, paradols. silver (AgNPs) derived from extracts could important non-toxic eco-friendly nanomaterial widespread In this study, AgNPs were biosynthesized using ethanolic extract rhizome their phytochemical, antioxidant, antibacterial, cytotoxic properties evaluated. UV–visible spectral analysis confirmed formation spherical AgNPs. FTIR revealed that NPs associated various functional biomolecules during stabilization. particle size SEM analyses range 80–100 nm, polydispersity index (PDI) 0.510, zeta potential −17.1 mV. purity crystalline nature by X-ray diffraction analysis. simple repeatable phyto-fabrication method reported here may used scaling up large-scale production ginger-derived NPs. A presence alkaloids, glycosides, flavonoids, phenolics, tannins, saponins, terpenoids, which serve active biocatalysts natural stabilizers metallic at low concentrations demonstrated cytotoxicity Vero cell lines 50% reduction viability 0.6–6 μg/mL. When evaluated biological activity, exhibited antioxidant antibacterial activity on several Gram-positive Gram-negative species, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus. suggests multi-drug-resistant bacteria. Ginger-derived have considerable development broad-spectrum antimicrobial anticancer medications, optimistic perspective medicine pharmaceutical industry.

Language: Английский

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Exploring the mechanisms of Yang Wei Shu granule for the treatment of chronic atrophic gastritis using UPLC-QTOF-MS/MS, network pharmacology, and cell experimentation

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119326 - 119326

Published: Jan. 1, 2025

Language: Английский

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Anticancer and cancer preventive activities of shogaol and curcumin from Zingiberaceae family plants in KG-1a leukemic stem cells

BMC Complementary Medicine and Therapies, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 28, 2025

Leukemic stem cells (LSCs) present a significant challenge in the treatment of leukemia patients because they exhibit drug-resistant phenotype, making them difficult to eliminate. Searching for new anticancer drug is crucial improving treatment. Plants from Zingiberaceae family are frequently used traditional medicines due their safety and accessibility. This study explores activity, cancer preventive properties, apoptosis inducing mechanisms active compounds derived these plants. Ten crude ethanolic extracts each plant were obtained using maceration techniques. The cytotoxicity all was assessed comparison drugs (cyclophosphamide, cytarabine, doxorubicin, idarubicin) MTT assay on cell lines (KG-1a, K562, A549, MCF-7, HeLa) peripheral blood mononuclear (PBMCs). Cancer prevention properties effective evaluated by measuring levels tumor necrosis factor-alpha (TNF-α), interleukin-2 (IL-2), nitric oxide (NO) commercial kits. Cell cycle death analyses conducted flow cytometry. Moreover, effects WT1 CD34 expressions, as well mechanism induced KG-1a cells, determined Western blotting. tests revealed that Curcuma longa, C. zedoaria, Zingiber officinale exhibited against while demonstrating lower impact PBMCs. longa zedoaria curcuminoids, those Z. shogaol gingerol. Notably, IC20 values curcuminoids reduced protein expression, thereby inhibiting proliferation. Furthermore, curcumin demonstrated ability arrest at G2/M phase induce through Akt pathway. These findings highlight promising

Language: Английский

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An overview of endometriosis and molecular target-based therapeutic approach

Middle East Fertility Society Journal, Journal Year: 2025, Volume and Issue: 30(1)

Published: March 20, 2025

Abstract Background Endometriosis has become a global concern. Fifty percent of the affected women infertile. Ten female population, which represents in their reproductive age and girls, is globally. It shows strong correlation with thyroid, endometrial, breast cancer. disrupts psychological, social, economic wellbeing sexual life women. Main body Modern hormonal therapy relies upon estrogen–progestin combinations. Other drugs include progestins, gonadotropin-releasing hormone agonists antagonists. Some patients remain non-responsive to these therapies, others show adverse effects such as intolerance, weight gain, acne, seborrhea. Similarly, surgery its own complications late bowel, ureteral perforations, recto-vaginal, uretero-vaginal fistulas. Neither modern therapeutic nor surgical approaches could alleviate endometriosis. Besides, cost treatment overburdening. This necessitates designing an alternative approach led identification molecular targets exploration different phytoconstituents that modulate targets. Conclusion Formulation containing apigenin, genistein, resveratrol, 5α-hydroxycostic acid, hydroxyisocostic anthocyanins, quercetin, naringenin, kaempferol, withaferin-A, ursolic shogaol, curcumin, demethoxycurcumin, capsaicin, ellagic 6‐paradol, 6‐gingerol, carnosic tuberostemonine-O, rosmarinic luteolin, granatin-B, licochalcone-A may be useful emdometriosis. formulation decrease proliferation ectopic endometrial stromal cells, invasion, vascularization, pain sensation, inflammation, gestational diabetes mellitus, fetal growth restriction. There increase fertility rate also. due ability regulate expression many VEGF-A/VEGFR2 pathway, p38MAPK/ERK-1/2/PI3K/protein kinase B (AKT), HIF-1α, IL-1β, IL-2, IL-6, IL-8, IL-10, IL-17A, IL-18, IL-33, TNF-α, NF-kB, IFN-γ, IGF-1-induced activation IGF-1R, ER-α, ER-β receptors, miR-95, miR-103, miRNA-138, miRNA-155, miR-183, miR-223, MMP-1, MMP-2, MMP-3, MMP-9, lncRNA-MEG3, lncRNA-H19, Ang-1 mRNA, Ang-2 mRNA urokinase plasminogen activator, secretion leptin, CD31, Tie-2, MCP-1 protein, HGF, Nrf2, HO1, Keap1, COX-2, PGE-2, MKNK1, human DNA TOP3A. However, further research required determine safety, compatibility, efficacy this formulation.

Language: Английский

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Potensi Sitotoksik Ekstrak Rizoma Jahe Merah (Zingiber officinale var. rubrum) terhadap Sel Kanker Payudara 4T1

Journal of Pharmaceutical And Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 706 - 715

Published: April 25, 2025

Latar Belakang: Kanker payudara 4T1 merupakan salah satu jenis kanker murine Triple-Negative (TNBC) yang memiliki kemampuan metastasis kuat. Senyawa metabolit tanaman strategi perlu dieksplorasi untuk mengembangkan pengobatan payudara. Rimpang jahe merah kandidat aktivitas antikanker. Sebelumnya, penelitian sitotoksik telah dilakukan terhadap sel Widr dan Hela. Namun, masih kurang, sehingga dikembangkan. Bahan Metode: Penelitian ini bertujuan mengetahui potensi secara in-vitro menggunakan uji MTT. Metode memperoleh ekstrak rimpang adalah dengan cara maserasi pelarut etanol 96% kemudian dianalisis senyawa sekundernya. Hasil: Hasil menunjukkan bahwa mengandung flavonoid, fenolik, alkaloid, saponin tanin. Sedangkan pada sitotoksik, doksorubisin baik nilai IC50 masing-masing sebesar 69,86 µg/mL 0,4 µg/mL. Ekstrak tergolong aktif sangat aktif. Kesimpulan: Dapat disimpulkan sebagai terapi mampu menghambat pertumbuhan hingga 50%. Oleh karena itu, berpotensi dikembangkan lebih lanjut agen kemoterapi.

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Zingiber officinale promotes autophagy and apoptosis in human oral cancer through the C/EBP homologous protein

Cancer Science, Journal Year: 2024, Volume and Issue: 115(8), P. 2701 - 2717

Published: June 18, 2024

Abstract The rhizome of Zingiber officinale ( Z. ), commonly known as ginger, has been characterized a potential drug candidate due to its antitumor effects. However, the chemotherapeutic effect ginger on human oral cancer remains poorly understood. In this study, we examined effects an ethanol extract rhizomes (ZOE) and identified components responsible for pharmacological activity. ZOE exerts inhibitory activity in by inducing both autophagy apoptosis simultaneously. Mechanistically, ZOE‐induced are attributed reactive oxygen species (ROS)‐mediated endoplasmic reticulum stress response. Additionally, two active ZOE, 1‐dehydro‐6‐gingerdione 8‐shogaol, which were sufficient stimulate initiation induction enhancing CHOP expression. These results suggest that induce ROS generation, upregulate CHOP, initiate apoptosis, hold promising therapeutics against cancer.

Language: Английский

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6-Shogaol Ameliorates Liver Inflammation and Fibrosis in Mice on a Methionine- and Choline-Deficient Diet by Inhibiting Oxidative Stress, Cell Death, and Endoplasmic Reticulum Stress

Molecules, Journal Year: 2024, Volume and Issue: 29(2), P. 419 - 419

Published: Jan. 15, 2024

Non-alcoholic steatohepatitis (NASH) is becoming an increasingly serious global health threat, distinguished by hepatic lipid accumulation, inflammation, and fibrosis. There a lack of approved pharmaceutical interventions for this disease, highlighting the urgent need effective treatment. This study explores hepatoprotective potential 6-shogaol, natural compound derived from ginger, in methionine- choline-deficient (MCD) dietary mouse model NASH. Male C57BL/6J mice were subjected to MCD diet 4 weeks induce NASH, with concurrent intraperitoneal administration 6-shogaol (20 mg/kg) three times week. While did not impact body weight, liver or it effectively mitigated injury, fibrosis diet-fed mice. Mechanistically, inhibited DNA oxidation, restored glutathione levels, regulated expression pro-oxidant antioxidant enzymes. Furthermore, apoptosis necroptosis, as indicated decrease TUNEL-stained cells downregulation apoptosis- necroptosis-associated proteins. Additionally, alleviated endoplasmic reticulum (ER) stress, demonstrated decreased molecules associated unfolded protein response pathways. These findings underscore therapeutic intervention NASH targeting pathways related oxidative cell death, ER stress.

Language: Английский

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Preventing Cancer by Inhibiting Ornithine Decarboxylase: A Comparative Perspective on Synthetic vs. Natural Drugs

Chemistry & Biodiversity, Journal Year: 2024, Volume and Issue: 21(4)

Published: Feb. 26, 2024

This perspective delves into the investigation of synthetic and naturally occurring inhibitors, their patterns inhibition, effectiveness newly utilized natural compounds as inhibitors targeting Ornithine decarboxylase enzyme. enzyme is known to target MYC oncogene, thereby establishing a connection between polyamine metabolism oncogenesis in both normal cancerous cells. ODC activation heightened activity are associated with tumor development numerous cancers fluctuations protein levels exert profound influence on cellular for inhibition or suppressing outlines efforts develop novel drugs, evaluate compounds, identify promising address gaps cancer prevention, highlighting potential designed moieties flavonoids alternatives. It also discusses enhanced inhibitors.

Language: Английский

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6-Shogaol Alleviates Neuronal Cell Excessive Autophagy and Calcium Overload in Cerebral Ischemia-Reperfusion Injury via Modulating miRNA-26a-5p/DAPK1 Signaling Axis

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 5, 2024

Objectives：Clarifying the Potential of 6-shogaol (6-SH) in Reducing Excessive Autophagy and Calcium Overload Neuronal Cells during Cerebral Ischemia-Reperfusion Injury via Regulation miRNA-26a-5p/DAPK1 Signaling Axis. Methods：In this study, oxygen-glucose deprivation/reoxygenation (OGD/R) HT22 cells was used to create an vitro model cerebral ischemia-reperfusion (CIRI). The following groups were created：normal control (NC), (OGD/R), model+6-shogaol treatment (6-SH+OGD/R), treatment+miRNA-26a-5p inhibitor negative (inhibitorNC+6-SH+OGD/R) (inhibitor+6-SH+OGD/R).Cell morphology observed under inverted microscope, cell viability assessed using CCK8 assay, neuronal ultrastructure examined transmission electron microscopy, intracellular calcium ion concentration measured flow cytometry, fluorescence intensity LC3 Beclin1 detected by immunofluorescence. Western blotting performed evaluate expression levels proteins related autophagy channels, Quantitative real-time PCR（RT-qPCR）was conducted measure gene miRNA-26a-5p DAPK1. Sprague Dawley rat CIRI established vivo utilizing suture-occluded approach. Three used: sham, (I/R), therapy (6-SH+I/R).TTC staining observe infarction, HE assess brain tissue pathology, immunofluorescence detect Beclin1, protein RT-qPCR determine Additionally, molecular docking techniques employed validate spontaneous binding 6-SH miRNA-26a-5p, dual luciferase reporter assays confirm targeting relationship between Results：The study showed that spontaneously bound assay confirmed can target regulate According outcomes experiments, dramatically improved OGD/R, reduced pathological damage, elevated downregulated DAPK1, excessive overload. ability downregulate DAPK1-mediated overload greatly diminished when blocked. Results experiments markedly decreased size infarct area SD rats MCAO/R, mitigated overload, relieved partially recovered function. It also Conclusion：6-SH modulates inhibit thereby attenuating neurons caused Injury.

Language: Английский

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