Cross-talks of GSH, mitochondria, RNA m6A modification, NRF2, and p53 between ferroptosis and cuproptosis in HCC: A review

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 302, P. 140523 - 140523

Published: Feb. 1, 2025

Language: Английский

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The promoting effect of the POU3F2/METTL16/PFKM cascade on glycolysis and tumorigenesis of hepatocellular carcinoma

Annals of Hepatology, Journal Year: 2025, Volume and Issue: unknown, P. 101776 - 101776

Published: Jan. 1, 2025

Deregulation of m

Language: Английский

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Organoids and spheroids: advanced in vitro models for liver cancer research

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 9, 2025

Liver cancer is a leading cause of cancer-related deaths worldwide, highlighting the need for innovative approaches to understand its complex biology and develop effective treatments. While traditional in vivo animal models have played vital role liver research, ethical concerns demand more human-relevant systems driven development advanced vitro models. Spheroids organoids emerged as powerful tools due their ability replicate tumor microenvironment facilitate preclinical drug development. are simpler 3D culture that partially recreate structure cell interactions. They can be used penetration studies high-throughput screening. Organoids derived from stem cells or patient tissues accurately emulate complexity functionality tissue. generated pluripotent adult cells, well specimens, providing personalized studying behavior responses. retain genetic variability original offer robust platform screening treatment strategies. However, both spheroids limitations, such absence functional vasculature immune components, which essential growth therapeutic The field modeling evolving, with ongoing efforts predictive reflect complexities human cancer. By integrating these tools, researchers gain deeper insights into accelerate novel

Language: Английский

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Enhancer transcription profiling reveals an enhancer RNA-driven ferroptosis and new therapeutic opportunities in prostate cancer

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: March 14, 2025

Abstract Enhancer RNAs (eRNAs), a subclass of non-coding transcribed from enhancer regions, have emerged as critical regulators gene expression; however, their functional roles in prostate cancer remain largely unexplored. In this study, we performed integrated chromatin accessibility and transcriptomic analyses using ATAC-seq RNA-seq on twenty pairs matched benign tissues. By incorporating immunoprecipitation sequencing data, identified subset differentially expressed eRNAs significantly associated with genes involved development oncogenic signaling pathways. Among these, lactotransferrin-eRNA ( LTF e) was markedly downregulated tissues, revealing its tumor-suppressive role. Mechanistically, e promotes the transcription target gene, lactotransferrin ), by interacting heterogeneous nuclear ribonucleoprotein F (HNRNPF) facilitating enhancer-promoter interactions. Furthermore, demonstrate that e- axis facilitates ferroptosis modulating iron transport. Notably, androgen receptor (AR) disrupts e-associated looping, leading to resistance. Therapeutically, co- administration AR inhibitor enzalutamide inducer RSL3 suppressed tumor growth, offering promising strategy for castration-resistant cancer. Collectively, study provides novel insights into mechanistic role cancer, highlighting epigenetic regulator potential therapeutic improved treatment outcomes.

Language: Английский

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RNA m6A modification in ferroptosis: implications for advancing tumor immunotherapy

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Sept. 28, 2024

Language: Английский

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RNA methyltransferase METTL16: from molecular mechanisms to therapeutic prospects in cancers

Cancer Letters, Journal Year: 2025, Volume and Issue: unknown, P. 217698 - 217698

Published: April 1, 2025

Language: Английский

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Summary of the mechanism of ferroptosis regulated by m6A modification in cancer progression

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: April 9, 2025

The most common form of internal RNA modification in eukaryotes is called n6-methyladenosine (m6A) methylation. It has become more and well-known as a research issue recent years since it alters metabolism involved numerous biological processes. Currently, m6A alteration offers new opportunities clinical applications intimately linked to carcinogenesis. Ferroptosis—a iron-dependent, lipid peroxidation-induced regulated cell death—was discovered. In the development cancer, an important factor. According newly available data, ferroptosis regulates tumor growth, cancer exhibits aberrant levels crucial regulatory components. On other hand, multiple roles tumors, relationship between m6A-modified malignancies quite intricate. this review, we first give thorough review functional methylation, focusing on molecular processes through regulation human progression metastasis, which are strongly associated initiation, progression, drug resistance. Therefore, clarify m6A-mediated providing strategy for treatment with substantial implications.

Language: Английский

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Identification of m6 A-regulated ferroptosis biomarkers for prognosis in laryngeal cancer

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 14, 2025

Laryngeal cancer (LC) is a malignant tumor that occurs in the larynx. N6-methyladenosine (m6A) RNA methylation, pivotal and prevalent epigenetic modification eukaryotic mRNA, intricately intertwines with ferroptosis, together, they play crucial role development of LC. Accordingly, further research on related molecular mechanisms pathology LC necessary. Weighted gene co-expression network analysis correlation were used to identify differentially expressed m6A-related ferroptosis genes The TCGA-HNSC GSE65858 datasets obtained from public databases. dataset consisted 110 primary oropharynx samples 12 control samples, while contained forty-eight samples. Univariate Cox least absolute shrinkage selection operator (LASSO) regression utilized for feature risk model construction dataset. was validated Then, nomogram built based independent prognostic factor identified using univariate multivariate Mutation analysis, immune-related drug sensitivity prediction applied analyze utility Additionally, qRT-PCR western blot performed detect TFRC, RGS4, FTH1 expression. Three biomarkers build LASSO algorithms. Receiver operating characteristic (ROC) verified accuracy model. Tumor Immune Dysfunction Exclusion (TIDE) Estimation STromal cells MAlignant Tumors Expression data (ESTIMATE) algorithm showed positive relationship between score TIDE or ESTIMATE score. Furthermore, found 19 chemotherapy drugs strongly correlated exhibited high expression levels 30 laryngeal carcinoma tissues cell lines. Notably, TFRC significantly associated patient prognosis. In Conclusion, FTH1, as m6A-regulated LC, providing insights into treatment

Language: Английский

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Mechanism of ferroptosis resistance in cancer cells

Cancer Drug Resistance, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Ferroptosis is an iron-dependent cell death characterized by increased intracellular lipid peroxidation. Inducing ferroptosis has shown significant potential in eliminating various malignancies. However, the effectiveness of ferroptosis-based treatments hampered intrinsic or acquired resistance some tumors. In this review, we delineate known mechanisms that regulate sensitivity and summarize therapeutic application inducers cancer. Additionally, discuss roles diverse signaling pathways contribute to cancer cells, including glutathione (GSH) coenzyme Q (CoQ) pathways, NFE2-like bZIP transcription factor 2 (NRF2) antioxidant response, iron metabolism. This emerging knowledge may serve as a foundation for developing novel anticancer strategies overcome resistance.

Language: Английский

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