Role of STAT3 in cancer cell epithelial‑mesenchymal transition (Review)

International Journal of Oncology, Journal Year: 2024, Volume and Issue: 64(5)

Published: March 13, 2024

Since its discovery, the role of transcription factor, signal transducer and activator 3 (STAT3), in both normal physiology pathology numerous diseases, including cancer, has been extensively studied. STAT3 is aberrantly activated different types fulfilling a critical cancer progression. The biological process, epithelial‑mesenchymal transition (EMT), indispensable for embryonic morphogenesis. During development EMT hijacked to confer motility, tumor cell stemness, drug resistance adaptation changes microenvironment. aim present review was outline recent advances knowledge EMT, which may contribute understanding function various cancer. Delineating underlying mechanisms associated with STAT3‑EMT signaling axis generate novel diagnostic therapeutic options treatment.

Language: Английский

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LncRNA NEAT1 promotes endometrial cancer cell proliferation, migration and invasion by regulating the miR-144-3p/EZH2 axis

Radiology and Oncology, Journal Year: 2019, Volume and Issue: 53(4), P. 434 - 442

Published: Nov. 20, 2019

Background Endometrial cancer (EC) is one of the most common gynaecological tumours in worldwide. Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes cell proliferation, migration and invasion EC cells. However, molecular mechanisms NEAT1 have not been fully clarified. We conducted this study to reveal function tissues lines. Materials methods Cancer adjacent were collected from patients. HEC-1A Ishikawa cells cultured vitro. expression was downregulated by transfecting small hairpin (shRNA) miR-144-3p overexpressed mimics. Cell proliferation detected MTT assay colony formation assay. abilities assessed transwell A dual-luciferase reporter used verify relationship among NEAT1, EZH2, miR-144-3p. The level EZH2 measured Western blot qPCR. Results highly expressed Knockdown inhibited Additionally, acted as a ceRNA miR-144-3p, leading upregulation. Overexpression suppressed Conclusions regulating miR-144-3p/EZH2 axis.

Language: Английский

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PDE4 subtypes in cancer

Oncogene, Journal Year: 2020, Volume and Issue: 39(19), P. 3791 - 3802

Published: March 20, 2020

Language: Английский

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Association of N6-methyladenine DNA with plaque progression in atherosclerosis via myocardial infarction-associated transcripts

Cell Death and Disease, Journal Year: 2019, Volume and Issue: 10(12)

Published: Dec. 4, 2019

Modification of the novel N6-methyladenine (m6A) DNA implicates this epigenetic mark in human malignant disease, but its role atherosclerosis (AS) is largely unknown. Here, we found that leukocyte level m6A not 5mC modification was decreased with increasing carotid plaque size and thickness 207 AS patients as compared 142 sex- age-matched controls. Serum low-density lipoprotein (LDL) levels were associated progression thickness. Both LDL also independently negatively related to level. Reduced further confirmed leukocytes endothelium western diet-induced mice oxidized-LDL (ox-LDL)-treated monocyte cells. Decreased closely upregulation AlkB homolog 1 (ALKBH1), demethylase m6A. Silencing ALKBH1 or hypoxia-inducible factor 1α (HIF1α) could rescue ox-LDL-increased MIAT, a hypoxia-response gene. Mechanically, ox-LDL induced HIF1α for transfer into nucleus. Nuclear bound ALKBH1-demethylated MIAT promoter transcriptionally upregulated expression. Therefore, elevated reduced level, which sensitive biomarker progression.

Language: Английский

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Non-Coding RNAs as Prognostic Markers for Endometrial Cancer

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(6), P. 3151 - 3151

Published: March 19, 2021

Endometrial cancer (EC) has been classified over the years, for prognostic and therapeutic purposes. In recent classification systems have emerging not only based on EC clinical pathological characteristics but also its genetic epigenetic features. Noncoding RNAs (ncRNAs) are as promising markers in several types, including EC, which their value is currently under investigation will likely integrate present tools protein coding genes. This review aims to underline importance of events tumorigenesis, by expounding upon role ncRNAs.

Language: Английский

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LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness

Cell Death and Disease, Journal Year: 2020, Volume and Issue: 11(11)

Published: Nov. 9, 2020

Abstract Resistance of chemotherapy is one causes recurrence and poor prognosis in patients with colorectal cancer (CRC). The role differentially expressed long non-coding RNA (lncRNA) 5-fluorouracil (5-Fu) resistance has not been fully elucidated. Here we observed that lncRNA NEAT1 was associated 5-Fu CRC. Our Functional studies showed promoted cells. In addition, A-TAC sequencing chromatin immunoprecipitation (ChIP) affected remodeling, increased the acetylation levels histones, their enrichment at promoters ALDH1 c-Myc, expression c-Myc. Taken together, our study suggested stemness by remodeling chromatin. finding provides a novel may provide new strategy for treatment CRC resistance.

Language: Английский

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MicroRNA-361-Mediated Inhibition of HSP90 Expression and EMT in Cervical Cancer Is Counteracted by Oncogenic lncRNA NEAT1

Cells, Journal Year: 2020, Volume and Issue: 9(3), P. 632 - 632

Published: March 5, 2020

Epithelial-mesenchymal transition (EMT) is a key process contributing to cervical cancer (CC) metastasis, and microRNAs (miRNAs) modulate the expression of genes implicated in EMT. However, accurate role miR-361 CC-associated EMT mechanisms underlying its function CC remains largely unknown. The functional roles cells were explored by series cell assays. Luciferase reporter assays used demonstrate potential interaction between miR-361, HSP90, long non-coding RNA (lncRNA) NEAT1. We detected reduction tissues compared with normal tissues, overexpression inhibited invasion phenotypes directly targeting activator HSP90. Additionally, we significantly higher levels HSP90 high predicted poorer prognosis. further identified NEAT1 as upregulated lncRNA was associated worse survival patients. repressed played an oncogenic sphere formation. Conclusions: These results demonstrated that targets inhibit features, functions suppresses induces formation cells, thus providing critical insights into molecular pathways operating this malignancy.

Language: Английский

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Over-expression of lncRNA TMEM161B-AS1 promotes the malignant biological behavior of glioma cells and the resistance to temozolomide via up-regulating the expression of multiple ferroptosis-related genes by sponging hsa-miR-27a-3p

Cell Death Discovery, Journal Year: 2021, Volume and Issue: 7(1)

Published: Oct. 23, 2021

A growing body of evidence suggests that long-chain non-coding RNA (lncRNA) plays an important role in the malignant biological behavior and drug resistance glioblastoma (GBM) cells. In this study, we analyzed potential mechanism lncRNA TMEM161B-AS1 GBM cells temozolomide (TMZ) resistance. Studies have found FANCD2 CD44 are significantly related to occurrence GBM, TMZ survival patients. Knockdown down-regulated expression by sponging hsa-miR-27a-3p, inhibited proliferation, migration, invasion promoted apoptosis, ferroptosis U87 U251 Down-regulation and/or over-expression hsa-miR-27a-3p CD44, tumor growth nude mice. These results demonstrated TMEM161B-AS1-hsa-miR-27a-3p-FANCD2/CD44 signal axis regulated findings were expected provide promising therapeutic targets for treatment glioma.

Language: Английский

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ncRNAInter: a novel strategy based on graph neural network to discover interactions between lncRNA and miRNA

Briefings in Bioinformatics, Journal Year: 2022, Volume and Issue: 23(6)

Published: Aug. 29, 2022

In recent years, many studies have illustrated the significant role that non-coding RNA (ncRNA) plays in biological activities, which lncRNA, miRNA and especially their interactions been proved to affect processes. Some silico methods proposed applied identify novel lncRNA-miRNA (LMIs), but there are still imperfections representation information extraction approaches, imply is room for further improving performances. Meanwhile, only a few of them accessible at present, limits practical applications. The construction new tool LMI prediction thus imperative better understanding relevant mechanisms. This study method, ncRNAInter, prediction. A comprehensive strategy an optimized deep learning algorithm graph neural network were utilized this study. ncRNAInter was robust showed performance 26.7% higher Matthews correlation coefficient than existing reputable human addition, its universal applicability dealing with LMIs from various species successfully identified associated diseases, verified effectiveness usability. All source code datasets freely available https://github.com/idrblab/ncRNAInter.

Language: Английский

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Molecular Interactions of the Long Noncoding RNA NEAT1 in Cancer

Cancers, Journal Year: 2022, Volume and Issue: 14(16), P. 4009 - 4009

Published: Aug. 19, 2022

As one of the best-studied long noncoding RNAs, nuclear paraspeckle assembly transcript 1 (NEAT1) plays a pivotal role in progression cancers. NEAT1, especially its isoform NEAT1-1, facilitates growth and metastasis various cancers, excluding acute promyelocytic leukemia. NEAT1 can be elevated via transcriptional activation or stability alteration cancers changing aggressive phenotype cancer cells. also secreted from other cells delivered to through exosomes. Hence, elucidating molecular interaction may shed light on future treatment cancer. Herein, we review function progression, explain how interacts with proteins, DNA promoter regions upregulate tumorigenic factors.

Language: Английский

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