RNA epigenetic modifications in digestive tract cancers: Friends or foes DOI Creative Commons

Zekai Nian,

Ming Deng, Lele Ye

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 206, P. 107280 - 107280

Published: June 22, 2024

Digestive tract cancers are among the most common malignancies worldwide and have high incidence mortality rates. Thus, discovery of more effective diagnostic therapeutic targets is urgently required. The development technologies to accurately detect RNA modification has led identification numerous chemical modifications in humans (epitranscriptomics) that involved occurrence digestive cancers. can cooperatively regulate gene expression facilitate normal physiological functions system. However, dysfunction relevant RNA-modifying enzymes ("writers," "erasers," "readers") lead Consequently, targeting dysregulated enzyme activity could represent a potent strategy for treatment In this review, we summarize widely studied roles mechanisms (m6A, m1A, m5C, m7G, A-to-I editing, pseudouridine [Ψ]) relation cancers, highlight crosstalk between modifications, discuss their interactions system microbiota during carcinogenesis. clinical significance novel methods based on also discussed. This review will help guide future research into resistant current therapeutics.

Language: Английский

Extracellular vesicles in gastric cancer: role of exosomal lncRNA and microRNA as diagnostic and therapeutic targets DOI Creative Commons
Chengyao Jiang, Jianjun Zhang, Wentao Wang

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 16, 2023

Extracellular vesicles (EVs), including exosomes, play a crucial role in intercellular communication and have emerged as important mediators the development progression of gastric cancer. This review discusses current understanding EVs, particularly exosomal lncRNA microRNA, cancer their potential diagnostic therapeutic targets. Exosomes are small membrane-bound particles secreted by both cells stromal within tumor microenvironment. They contain various ncRNA biomolecules, which can be transferred to recipient promote growth metastasis. In this review, we highlighted importance microRNA Exosomal lncRNAs been shown regulate gene expression interacting with transcription factors or chromatin-modifying enzymes, binding target mRNAs. We also discuss use microRNAs biomarkers for isolated from bodily fluids, blood, urine, saliva. specific molecules that reflect molecular characteristics tumor, making them promising candidates non-invasive tests. Finally, targeting strategy were reviewed wee. Inhibition exosomes has suppress metastasis preclinical models. conclusion, article provides an overview suggest further research into these could lead new tools strategies deadly disease.

Language: Английский

Citations

12
Non-coding RNAs’ function in cancer development, diagnosis and therapy DOI Open Access
Xinyi Zhang, Xiaoqing Xu, Jiajia Song

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 167, P. 115527 - 115527

Published: Sept. 24, 2023

While previous research on cancer biology has focused genes that code for proteins, in recent years it been discovered non-coding RNAs (ncRNAs)play key regulatory roles cell biological functions. NcRNAs account more than 95% of human transcripts and are an important entry point the study mechanism development. An increasing number studies have demonstrated ncRNAs can act as tumor suppressor or oncogenes to regulate development at epigenetic level, transcriptional well post-transcriptional level. Because importance cancer, most clinical trials explore whether be used new biomarkers therapies. In this review, we focus including microRNAs (miRNAs), long (lncRNAs), circle (circRNAs), PIWI interacting (piRNAs), tRNA different types application these identification relevant therapeutic targets biomarkers. Graphical abstract drawn by Fidraw.

Language: Английский

Citations

12
Circular RNA EIF3I promotes papillary thyroid cancer progression by interacting with AUF1 to increase Cyclin D1 production DOI
Xuelin Yao, Hanyuan Liu, Zhen Wang

et al.

Oncogene, Journal Year: 2023, Volume and Issue: 42(43), P. 3206 - 3218

Published: Sept. 11, 2023

Language: Английский

Citations

11
Comprehensive analysis of IGF2BP3 with expression features, prognosis, immune modulation and stemness in hepatocellular carcinoma and pan-cancer DOI Creative Commons
Sha Qin,

Haoer Jin,

Yan Li

et al.

Journal of Cancer, Journal Year: 2024, Volume and Issue: 15(9), P. 2845 - 2865

Published: Jan. 1, 2024

Insulin like growth factor 2 mRNA binding protein 3 (IGF2BP3) is a critical m6A reader.It encodes proteins that contain several KH domains, which are important in RNA binding, synthesis and metabolism.Lots of researches have studied the malignant potential readers tumors.However, biological functional analysis IGF2BP3 hepatocellular carcinoma (HCC) pan-cancer not comprehensive.In this study, we used bioinformatics approach to comprehensively analyze significance HCC through analyzing its expression, mutation, prognosis, protein-protein interaction (PPI) network, enrichment, correlation with ferroptosis, stemness as well immune modulation HCC.IGF2BP3 presented negative ferroptosis molecule NFE2L2, positive SLC1A5 checkpoint HAVCR2.In addition, also analyzed prognosis pan-cancer, revealing prognostic value variety tumors.Finally, verified functions various experiments.The data showed may enhance proliferation, colony formation invasion capacities cells, mainly positively correlated expression level marker SOX2.In conclusion, had be new perspective biomarker forecasting response, or even pan-cancer.

Language: Английский

Citations

4
RNA demethylase FTO participates in malignant progression of gastric cancer by regulating SP1-AURKB-ATM pathway DOI Creative Commons

Xueliang Zeng,

Yao Lu,

Taohui Zeng

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: July 2, 2024

Abstract Gastric cancer (GC) is the 5 th most prevalent and 4 primary cancer-associated mortality globally. As first identified m6A demethylase for removing RNA methylation modification, fat mass obesity-associated protein ( FTO ) plays instrumental roles in development. Therefore, we study biological functions oncogenic mechanisms of GC tumorigenesis progression. In our study, expression obviously upregulated tissues cells. The upregulation associated with advanced nerve invasion, tumor size, LNM, as well poor prognosis patients, promoted cell viability, colony formation, migration invasion. Mechanistically, targeted specificity 1 Aurora Kinase B, resulting phosphorylation ataxia telangiectasia mutated P38 dephosphorylation P53. conclusion, promotes progression by regulating SP1-AURKB-ATM pathway, which may highlight potential a diagnostic biomarker patients’ therapy response prognosis.

Language: Английский

Citations

4
CircPRMT5, a Potential Salivary Biomarker, Facilitates the Progression of Head and Neck Squamous Cell Carcinoma via the IGF2BP3-SERPINE1 Pathway DOI Creative Commons
Siqi Jiang, Linlin Ou, Yueqi Wang

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 1597 - 1613

Published: Feb. 1, 2025

Purpose: Circular RNAs (circRNAs) are associated with the progression of tumors and hold promise as potential biomarkers for liquid biopsy. Among these, role circPRMT5 in head neck squamous cell carcinoma (HNSCC) remains to be elucidated. This study aims examine underlying mechanisms HNSCC assess its diagnostic value saliva exosomes. Methods: The expression clinical significance were investigated. Both vitro vivo studies performed elucidate biological HNSCC. RNA sequencing was utilized identify downstream mechanisms. To evaluate validate these mechanisms, Western blotting, RNA-FISH, immunofluorescence, immunohistochemistry, RIP, rescue experiments employed. Finally, salivary exosomes isolated, levels assessed using qRT-PCR. Results: upregulation tissues identified correlated cervical lymph node metastasis advanced T stage. manifested that promoted proliferation Mechanistically, demonstrated directly bind stabilize insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3), which, subsequently, binds stabilizes serpin family E member 1 (SERPINE1) mRNA, thereby enhancing SERPINE1 expression. Furthermore, indicated proliferative, invasive, migratory effects dependent on involvement IGF2BP3 SERPINE1. Notably, significantly elevated patients, exhibiting substantial value. Conclusion: CircPRMT5 exhibits significant utility through plays a crucial promoting via IGF2BP3-SERPINE1 pathway. These findings highlight noninvasive biomarker therapeutic target patients Keywords: cancer, circPRMT5, IGF2BP3, SERPINE1,

Language: Английский

Citations

0
Knockdown of SLC7A5 inhibits malignant progression and attenuates oxaliplatin resistance in gastric cancer by suppressing glycolysis DOI Creative Commons
Yan Zhang, Jian Cao,

Zheng Yuan

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: March 25, 2025

Abstract Background Chemotherapy resistance is a major challenge in the treatment of intermediate and advanced gastric cancer (GC). This study aimed to recognize oxaliplatin resistance-related genes (OXARGs) GC explore their role mechanism GC. Methods OXARGs with prognostic value were analyzed using data from GEO TCGA databases. RT-qPCR WB assay applied verify expression MT2A, NOTCH1 SLC7A5 oxaliplatin-resistant cells (HGC27R MKN45R). The effect on malignant phenotype was verified by CCK-8, EDU, TUNEL, colony formation, wound healing, transwell assay, tumor bearing experiments assay. Results Bioinformatics analysis experimental validation indicate that target for oxaliplatin-resistance Knockdown obviously decreased viability, migration, invasion vitro growth vivo. It also increased apoptosis levels BAX expression, reduced BCL2, MMP 2 MMP9. Additionally, knockdown enhanced sensitivity both Furthermore, downregulated HK2, LDHA, Glut1, PDK1 vivo vitro, leading extracellular glucose lactate levels. However, glutathione significantly attenuated regulatory cells. Trial registration Not Applicable. Conclusion inhibits progression attenuates suppressing glycolysis.

Language: Английский

Citations

0
Circular RNAs modulate cancer drug resistance: advances and challenges DOI Open Access

Jinghan Hua,

Zhe Wang, Xiangfei Cheng

et al.

Cancer Drug Resistance, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Acquired drug resistance is a main factor contributing to cancer therapy failure and high mortality, highlighting the necessity develop novel intervention targets. Circular RNAs (circRNAs), an abundant class of RNA molecules with closed loop structure, possess characteristics including stability, which provide unique advantages in clinical application. Growing evidence indicates that aberrantly expressed circRNAs are associated against various treatments, targeted therapy, chemotherapy, radiotherapy, immunotherapy. Therefore, targeting these aberrant may offer strategy improve efficiency therapy. Herein, we present summary most recently studied their regulatory roles on resistance. With advances artificial intelligence (AI)-based bioinformatics algorithms, could emerge as promising biomarkers targets

Language: Английский

Citations

0
METTL3-dependent m6A methylation of circCEACAM5 fuels pancreatic cancer progression through DKC1 activation DOI Creative Commons
Jie Zhang, Wenxue Sun, Wenda Wu

et al.

Cellular and Molecular Life Sciences, Journal Year: 2025, Volume and Issue: 82(1)

Published: March 27, 2025

Language: Английский

Citations

0
Targeted delivery of circPDHK1 siRNA via aptamer functionalized lipid nanoparticles inhibits ccRCC growth and migration DOI
H Ning, Yan Jiang, Binbin Li

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 125666 - 125666

Published: April 1, 2025

Language: Английский

Citations

0