Composition of methane cycle microbial communities in the upper layers of bottom sediments of the Kara sea DOI

A. L. Bryukhanov,

V. S. Sevastyanov, М. Д. Кравчишина

et al.

Геохимия, Journal Year: 2024, Volume and Issue: 69(6), P. 511 - 519

Published: Dec. 17, 2024

A study of the distribution methane cycle microbial communities in upper layers bottom sediments above large hydrocarbon reservoirs South Kara petroleum region West Siberian Province revealed presence these both aerobic methanotrophic bacteria and anaerobic methanogenic archaea, as well numerous heterotrophic microorganisms various phylogenetic groups. Research was carried out Baydaratskaya Bay east Pukhuchan Depression (southern part Sea). Aerobic belonged to families Methyloligellaceae, Methylophagaceae Methylomonaceae were detected surface oxidized (0–2 cm, Eh from 60 175 mV) sediments. Moreover, representatives Methyloligellaceae found quite significant amount (1.52–2.61 % all 16S rRNA gene sequence reads) at studied stations Sea. In subsurface reduced (18–20 −63 –246 mV), archaea dominated by order Methanomassiliicoccales (up 3.3 reads). Methanogenic orders Methanofastidiosales, Methanobacteriales Methanomicrobiales also discovered. addition, aerobic/facultative Methylococcaceae Methylomonadaceae on sediments, but relative abundance (in percentage reads their total number) extremely low there. Apparently, oxidation Sea, where its concentration most dramatically increased with sediment depth, occurs predominantly under conditions layers, diversity methanotrophs is higher.

Language: Английский

Network analyses unraveled the complex interactions in the rumen microbiota associated with methane emission in dairy cattle DOI Creative Commons

Xiaoxing Ye,

Goutam Sahana, Mogens Sandø Lund

et al.

Animal Microbiome, Journal Year: 2025, Volume and Issue: 7(1)

Published: March 11, 2025

Methane emissions from livestock, particularly dairy cattle, represent a significant source of greenhouse gas, contributing to the global climate crisis. Understanding complex interactions within rumen microbiota that influence methane is crucial for developing effective mitigation strategies. This study employed Weighted Gene Co-expression Network Analysis investigate emissions. By integrating extensive sequencing data with precise emission measurements in 750 Holstein our research identified distinct microbial communities and their associations production. Key findings revealed blue module network analysis was significantly correlated (0.45) In this module, taxa included genera Prevotella Methanobrevibactor, along species such as brevis, ruminicola, baroniae, bryantii, Lachnobacterium bovis, Methanomassiliicoccus luminyensis are key components drive networks. However, absence metagenomics difficult reveal deeper level functional profiles. The application provided comprehensive understanding microbiota-methane relationship, serving an innovative approach microbiota-phenotype association studies cattle. Our underscore importance microbiota-trait microbiota-microbiota related systematic production offers information on management mitigating environmental impact cattle population.

Language: Английский

Citations

0
The inclusion of insect meal from Hermetia illucens larvae in the diet of laying hens (Hy-line Brown) affects the caecal diversity of methanogenic archaea DOI Creative Commons
Tiziana Maria Mahayri, Jakub Mrázek, Fulvia Bovera

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105037 - 105037

Published: March 1, 2025

Language: Английский

Citations

0
Multi-Omics Reveal Microbial Succession and Metabolomic Adaptations to Flood in a Hypersaline Coastal Lagoon DOI Creative Commons
Christopher Keneally, Daniel Chilton, Tyler Dornan

et al.

Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123511 - 123511

Published: March 1, 2025

Language: Английский

Citations

0
Tea Polyphenols Inhibit Methanogenesis and Improve Rumen Epithelial Transport in Dairy Cows DOI Creative Commons
Zhanwei Teng,

Shuai Liu,

Lijie Zhang

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(17), P. 2569 - 2569

Published: Sept. 4, 2024

This study systematically investigated the effects of tea polyphenols on methane (CH4) production and rumen epithelial cell transport capability in cattle using both vitro animal experiments, employing multi-omics techniques. The results demonstrated that, compared to control group, significantly reduced CH4 acetate/propionate ratio (p < 0.05). Tea by inhibiting relative abundance unclassified_d_Archaea methanogens protozoa Pseudoentodinium g__Balantioides. experiments showed that increased concentrations T-AOC GSH-PX bovine blood In addition, microbial groups such as Rikenellaceae_RC9_gut_group, Ruminococcaceae_NK4A214_group, Butyrivibrio_2 were enriched ruminal fluid polyphenol group proteomic indicated significant upregulation proteins COIII, S100A8, FABP1, SLC2A8, SLC29A1 0.05) downregulation including HBB, RAB4A, RBP4, LOC107131172, HBA, ZFYVE19 0.05), with FABP1 showing a positive correlation propionate concentration, RAB4A had negative Overall, modulate composition within rumen, enhancing host’s capacity for volatile fatty acids.

Language: Английский

Citations

2
A metagenomic catalogue of the ruminant gut archaeome DOI Creative Commons
Jiandui Mi, Xiaoping Jing,

Chouxian Ma

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 7, 2024

While the ruminant gut archaeome regulates microbiota and hydrogen balance, it is also a major producer of greenhouse gas methane. However, diversity within gastrointestinal tract (GIT) animals worldwide remains largely underexplored. Here, we construct catalogue 998 unique archaeal genomes recovered from GITs ruminants, utilizing 2270 metagenomic samples across 10 different species. Most (669/998 = 67.03%) belong to Methanobacteriaceae Methanomethylophilaceae (198/998 19.84%). We recover 47/279 previously undescribed at strain level with completeness >80% contamination <5%. investigate biogeography various ruminants demonstrate that compositional similarities vary significantly by breed location. The contains 42,691 protein clusters, clustering methanogenic pathway analysis reveal strain- host-specific dependencies among animals. find archaea potentially carry antibiotic metal resistance genes, mobile genetic elements, virulence factors, quorum sensors, complex viromes. Overall, this substantial repository for recourses, providing potential advancing our understanding ecology discovering strategies regulate methane production in ruminants. employing 2,270 species, authors archaeome, comprised nonredundant genomes, expanding knowledge on Archaea compositions, diversity, functions

Language: Английский

Citations

2
Red seaweed supplementation suppresses methanogenesis in the rumen, revealing potentially advantageous traits among hydrogenotrophic bacteria DOI Creative Commons
Pengfan Zhang, Breanna M. Roque,

Pedro Romero

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 7, 2024

Macroalgae belonging to the genus Asparagopsis have been shown reduce production of methane (CH4) during rumen fermentation, while increasing feed efficiency when added cattle. However, little is known about how microbial community in responds supplementation, and changes microbiome may contribute shifts function ultimately hosts phenotype. In this study, we generated analyzed metagenomic metatranscriptomic data from associated with fluid collected two cohorts lactating dairy cows, one fed a diet supplemented armata (treatment) another same without A. supplementation (control). The reduction CH4 emission animals that received was coupled qualitative decrease relative archaeal abundance significant transcription methanogenesis pathways. Additionally, genes for complex carbon catabolism re-organization expression profile catabolic at species level observed treated animals. Increased H2 production, consequence suppression, increase hydrogenases mediate hydrogenotrophic metabolism treatment group. Analysis metatranscriptome identified single uncultured bacterial (a Duodenibacillus sp.) as dominant driver transcriptional change. Comparative genomic analysis between sp. other organisms revealed metabolic traits provide competitive advantage scavenging. These findings an initial understanding microbiota respond promising reducing additive, serve model understand alternative stable states produce less animal productivity.

Language: Английский

Citations

1
Harnessing meta-omics to unveil and mitigate methane emissions in ruminants: Integrative approaches and future directions DOI
Yuchao Zhao,

Jian Tan,

Luoyun Fang

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175732 - 175732

Published: Aug. 24, 2024

Language: Английский

Citations

1
Composition of Methane Cycle Microbial Communities in the Upper Layers of Bottom Sediments of the Kara Sea DOI

A. L. Bryukhanov,

V. S. Sevastyanov, М. Д. Кравчишина

et al.

Geochemistry International, Journal Year: 2024, Volume and Issue: 62(6), P. 609 - 617

Published: June 1, 2024

Language: Английский

Citations

0
Composition of methane cycle microbial communities in the upper layers of bottom sediments of the Kara sea DOI

A. L. Bryukhanov,

V. S. Sevastyanov, М. Д. Кравчишина

et al.

Геохимия, Journal Year: 2024, Volume and Issue: 69(6), P. 511 - 519

Published: Dec. 17, 2024

A study of the distribution methane cycle microbial communities in upper layers bottom sediments above large hydrocarbon reservoirs South Kara petroleum region West Siberian Province revealed presence these both aerobic methanotrophic bacteria and anaerobic methanogenic archaea, as well numerous heterotrophic microorganisms various phylogenetic groups. Research was carried out Baydaratskaya Bay east Pukhuchan Depression (southern part Sea). Aerobic belonged to families Methyloligellaceae, Methylophagaceae Methylomonaceae were detected surface oxidized (0–2 cm, Eh from 60 175 mV) sediments. Moreover, representatives Methyloligellaceae found quite significant amount (1.52–2.61 % all 16S rRNA gene sequence reads) at studied stations Sea. In subsurface reduced (18–20 −63 –246 mV), archaea dominated by order Methanomassiliicoccales (up 3.3 reads). Methanogenic orders Methanofastidiosales, Methanobacteriales Methanomicrobiales also discovered. addition, aerobic/facultative Methylococcaceae Methylomonadaceae on sediments, but relative abundance (in percentage reads their total number) extremely low there. Apparently, oxidation Sea, where its concentration most dramatically increased with sediment depth, occurs predominantly under conditions layers, diversity methanotrophs is higher.

Language: Английский

Citations

0