Eco-friendly RP-HPLC approach for simultaneously estimating the promising combination of pentoxifylline and simvastatin in therapeutic potential for breast cancer: Appraisal of greenness, whiteness, and Box–Behnken design DOI Creative Commons
Samar M. Mahgoub, Maha A. Alwaili, Hassan A. Rudayni

et al.

Green Processing and Synthesis, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

Abstract Breast cancer affects millions of women worldwide. This study explores the potential combining pentoxifylline (PTX) and simvastatin (SIM) as a treatment for breast cancer. We aimed to develop six sustainability tools using green white metrics evaluate environmental impact reversed-phase high-performance liquid chromatography (RP-HPLC) methods analyzing separating PTX SIM in their pure forms. The include analytical GREEnness, procedure index, Complexgreen greenness metric sample preparation, blue applicability grade RGB 12 algorithm. For separation, we used Novapack C8 column (15 × 0.46 cm, 5 µm) at 25°C. injection volume was 5.0 µL, wavelength set 210 nm, total runtime min. identified optimal chromatographic conditions efficiently Box–Behnken design with minimal trials. investigated effects three factors on retention time resolution: acetonitrile ratio, pH, flow rate. overlay plots 60:40 ratio (v/v) bi-distilled water forecast most effective mobile phase. calibration curves showed correlation value over 0.999 within range 5–60 µg·mL −1 . recovery rates ranged from 99.9% 100.2%, indicating high accuracy. Our RP-HPLC technique proves be reliable efficient simultaneous estimation multiple anticancer drugs. evaluated this approach metrics, recommended method has been thoroughly validated according International Council Harmonisation guidelines, making it highly new formulations.

Language: Английский

Greens assessment of RP-UPLC method for estimating Triamcinolone Acetonide and its degraded products compared to Box-Behnken and Six Sigma designs DOI Creative Commons
Hossam M. Nassef, Hoda A. Ahmed, Mohamed A. El‐Atawy

et al.

Green Chemistry Letters and Reviews, Journal Year: 2024, Volume and Issue: 17(1)

Published: Jan. 7, 2024

A study on green analytical chemistry aims to develop eco-friendly alternatives hazardous substances and minimize waste generation. The thoroughly examined various tools determine their greenness. newly validated RP-UPLC method was then employed quantitatively detect Triamcinolone Acetonide (TRA) in a creamy pharmaceutical formulation using UPLC BEH C18 (50 × 2.1 mm, 1.7μm) column for analysis, with detection wavelength set at 240 nm. Box-Behnken design developed efficiently the optimal chromatographic conditions while minimizing required trials. effectively assessed impact of three independent variables responses, including retention time, tailing factor, theoretical plates. ethanol ratio, pH levels, flow rate were meticulously tested optimize experimental conditions. utilization desirability overlay plots, along mobile phase purified water volumetric ratio 30:70 adjustment 5.0. Calibration curves constructed assess linearity TRA within concentration range 2.5-50 µg/mL, yielding correlation coefficients (r = 0.9999). accuracy through recovery studies acceptable 99.6-101.2%. specificity has been by conducting forced degradation per ICH guidelines.

Language: Английский

Citations

16
A novel Six Sigma approach and eco-friendly RP-HPLC technique for determination of pimavanserin and its degraded products: Application of Box–Behnken design DOI Creative Commons
Hossam M. Nassef, Hoda A. Ahmed, Ali H. Bashal

et al.

Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 43(1)

Published: Jan. 1, 2024

Abstract A green analytical chemistry study attempts to generate environmentally friendly alternatives dangerous compounds while reducing waste output. The thoroughly analyzed eight tools determine their greenness. Given its importance in treating hallucinations and delusions produced by Parkinson’s disease psychosis, it is critical have a reliable precise method for identifying pimavanserin (PVS) both pure form pharmaceutical formulations. current used straightforward approach forced degradation experiments establish novel reversed-phase high-performance liquid chromatography evaluating stability. was executed on the Agilent Zorbax Eclipse Plus C18 column (100 × 4.6 mm, 3.5 μm particle size) with ultraviolet detection at 226 nm. Box–Behnken design ultimate solution optimal chromatographic conditions timely efficient manner, minimal trials required. investigated impact of three factors: acetonitrile ratio, oven temperature, flow rate various responses, namely, retention time, tailing factor, theoretical plates. Desirability overlay plots were utilized forecast best mobile phase containing buffer solution: acetonitrile: tetrahydrofuran ratio (65: 20: 15, v/v/v), which proved highly effective experiments. Linearity conducted PVS 3–50 µg·mL −1 range an R 2 coefficient determination 0.9997. had quantification limits 1.1 , respectively, indicating significant correlation between variables studied. PVS’s recovery percentage determined be 101.30%. We also Six Sigma lean technique ensure precision productivity. tested acid, base, oxidative hydrolysis, photodegradation, heat, as per International Council Harmonisation guidelines. highest obtained from hydrolysis thermal degradation.

Language: Английский

Citations

15
Micellar HPLC and UV Methods with Time Programming for Synchronically Quantifying Gatifloxacin and Its Preservative in Eye Drops: Appraisal of Ecological Impact DOI Creative Commons
Maha A. Alwaili, Fahad M. Alminderej, Bandar R. Alsehli

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Modern society is increasingly reliant on sustainable solutions. We suggest a creative study that meets the standards of sustainability in analytical chemistry. The objective to promote eco-friendly methods for concurrently detecting Gatifloxacin (GAT) and benzalkonium chloride (BEN) ocular Using sustained mobile phase flow rate 0.8 mL/min-1 acidic water: 10% 1-butanol water (40:60, v/v), GAT was retained 2.242 min at 287 nm, followed by BEN homologues 215 nm next 2.982 4.201 min. This process simple, quick, precise. method demonstrated peak symmetry, low processing times, good resolution, correlation values 0.999. For GAT, linearity ranged from 0.001 0.023 mgmL-1, while BEN, it 0.003 0.060 mgmL-1 HPLC system, UV range 0.005-0.03 all drugs. A unique feature this integration multiple assessment tools, such as AGREE, AGREEprep, ComplexGAPI, ESA, BAGI, NEMI pictograms, providing an exhaustive appraisal methods' environmental impact enhancing robustness findings. In addition, cost-effective alternatives were explored using solvent complementary spectrophotometric procedures mean centering ratio spectra (MCR). Each technique showed respectable accuracy precision (RSD ≤ 2%) high r2 > 0.9990. proposed methodologies provide inexpensive, conventional approaches, promoting less harmful future quality control moving chemistry closer more methods.

Language: Английский

Citations

0
Eco-Friendly Optimised Chromatographic Method Technique Simultaneous Determinationof Hydroquinone, Fluocinolone Acetonide, Methyl Paraben, and Propyl Paraben in Pharmaceutical Formulations: Box-Behnken Design, Lean Six Sigma, Greenness Profile Assessment DOI

Islam A. Al-fattah,

Doaa G.M Mahmoud

Published: Jan. 1, 2025

Language: Английский

Citations

0
Rapid screening of cefotaxime resistance in Escherichia coli isolates by liquid chromatography with absorbance detection. DOI

Yamima Tasnim,

Hanin Diab, Sumon Sarkar

et al.

Journal of Chromatography B, Journal Year: 2025, Volume and Issue: 1256, P. 124565 - 124565

Published: March 19, 2025

Language: Английский

Citations

0
Green RP-HPLC method for simultaneous quantification of epigallocatechin-3-gallate and rosmarinic acid in lipid-based nanocarriers and biological fluids: Quality by Design-driven optimization and lean six sigma approach DOI Creative Commons
Rahul Koli,

V.S. Mannur

Green Analytical Chemistry, Journal Year: 2024, Volume and Issue: 11, P. 100153 - 100153

Published: Sept. 14, 2024

Language: Английский

Citations

3
Recent applications of Analytical Quality-by-Design methodology for chromatographic analysis: A review DOI

Doan Thanh Xuan,

Hue Minh Thi Nguyen,

Vu Dang Hoang

et al.

Chemometrics and Intelligent Laboratory Systems, Journal Year: 2024, Volume and Issue: 254, P. 105243 - 105243

Published: Oct. 10, 2024

Language: Английский

Citations

1
Innovative UPLC technique for concurrent quantification of etofenamate and benzyl nicotinate in the presence of methylparaben and benzyl alcohol in their topical cream: Greens, white, and Six Sigma methodologies DOI
Osama A. Mahmoud,

Ahmed A. Omran,

Hosni A. Gomaa

et al.

Biomedical Chromatography, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 14, 2024

Abstract The efficacious treatment of muscle and joint pain relies heavily on etofenamate (ETO) benzyl nicotinate (BN), which possess robust anti‐inflammatory pain‐relieving properties when paired with methylparaben (MP) or alcohol (BA). In this study, we have established validated innovative RP‐UPLC methods for assessing ETO BN in the presence MP BA their dosage forms, employing eight green tools to evaluate eco‐friendliness effectiveness. Reversed phase‐ultra‐performance liquid chromatography (RP‐UPLC) technique employs a flow rate 0.3 mL/min Waters Acquity UPLC BEH Column (C18, 1.7 μm, 100 mm × 2.1 mm), detection at 254 nm using photo diode array (PDA) detector mobile phase 0.05 M KH 2 PO 4 buffer, acetonitrile, methanol (50:15:35, v/v/v) adjusted pH 6.0 0.2% triethylamine. For ETO, BN, MP, BA, calibration curves were linear ranged from 0.005 1.0, 0.001 0.2, 0.002 0.08, 0.0001 0.1 mg/mL, respectively. correlation value was 0.9999, accuracy findings 98.81% 100.56%. Consequently, methodology has been successfully implemented assay testing pharmaceuticals demonstrating high selectivity these approaches. present study presents Blue Applicability Grade Index (BAGI), an approach that complements metrics practical white analytical chemistry. According International Council Harmonisation (ICH) criteria, procedures effectively validated.

Language: Английский

Citations

0
VYUŽITÍ CHROMATOGRAFICKÝCH KOLON S NÍZKÝM ZRNĚNÍM PŘI ANALÝZE REZIDUÍ β-LAKTAMOVÝCH A TETRACYKLINOVÝCH ANTIBIOTIK / THE USE OF SUB-2 μM CHROMATOGRAPHIC COLUMNS IN THE ANALYSIS OF β-LACTAM AND TETRACYCLINE ANTIBIOTIC RESIDUES DOI
Eva Dadáková, Kateřina Matějková,

Hana Kadlecová

et al.

Published: Jan. 1, 2024

Citations

0
A Green Approach: Optimization of the UPLC Method Using DoE Software for Concurrent Quantification of Pioglitazone and Dapagliflozin in a SNEDDS Formulation for the Treatment of Diabetes DOI Creative Commons
Ehab M. Elzayat, Abdelrahman Y. Sherif, Mohamed W. Attwa

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(45), P. 45011 - 45024

Published: Nov. 1, 2024

Clinical trials revealed that pioglitazone (PGZ) and dapagliflozin (DGZ) not only maintain normal blood glucose levels but also reduce complications of diabetes mellitus. To meet the demand for simultaneous measurement these drugs in fixed combinations, an optimized green UPLC method is required. The present study utilized Design Experiments (DoE) software to optimize analytical parameters drug analysis. was validated its linearity, accuracy, precision. Furthermore, content estimated different pharmaceutical dosage forms. Finally, Analytical Greenness (AGREE) assess environmental sustainability method. Drugs were successfully separated using conditions on C18 Acquity BEH column (2.1 mm × 100 mm, 1.7 μm) at a temperature 45 °C. mobile phase consisted ethanol 9 mM ammonium formate buffer (43.7:56.3), with elution carried out flow rate 0.246 mL/min. showed excellent linearity (R2 > 0.999), accuracy (92.45–109.25%), good precision (RSD < 6.27%) both drugs. In addition, able determine within marketed form accurately. developed prioritized eco-friendliness by solvents minimize negative impact environment. provides reliable accurate approach estimate PGZ DGZ treatment combination. It promotes sustainable lab practices paves way methods new dose combinations.

Language: Английский

Citations

0