Human schistosomiasis

The Lancet, Journal Year: 2025, Volume and Issue: 405(10479), P. 658 - 670

Published: Feb. 1, 2025

Language: Английский

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Schistosoma mansoni x S. haematobium hybrids frequently infecting sub-Saharan migrants in southeastern Europe: Egg DNA genotyping assessed by RD-PCR, sequencing and cloning

PLoS neglected tropical diseases, Journal Year: 2025, Volume and Issue: 19(3), P. e0012942 - e0012942

Published: March 31, 2025

Background Globalization and neglected tropical diseases (NTDs) are increasingly closely linked. In recent years, Spain Southern Europe experiencing a considerable increase in the influx of migrants infected by NTDs, mainly from West African countries. This study focuses on imported schistosomiasis entry into hetero-specific hybrids between two human species, Schistosoma mansoni S. haematobium, causing intestinal urogenital respectively. Methodology/principal findings Individualized genetic identification molecular analysis using RD-PCR, sequencing cloning nuclear rDNA mtDNA 134 eggs was performed, including 41 lateral-spined 84 terminal-spined urine, nine stools. These were recovered six migrant males Senegal, Guinea-Bissau, Côte d’Ivoire Mali, who shared ectopic shedding -like their urine. A high hybridization complexity detected these patients, involving three species. The patients x haematobium eggs, also curassoni haematobium- like eggs. SmxSh mostly urine (94.59%), whereas feces detection those less frequent (5.41%). Conclusions/significance contributes to: (i) better understanding heterospecific point view; (ii) it shows frequency with which they entering non-endemic countries, such as consequently Europe; (iii) determines diversity hybrid haplotypes that can occur within single patient, e.g., up to types species different haplotypes; (iv) provides information be considered clinical presentations, diagnosis, responses treatment epidemiological impact relation possible transmission establishment areas.

Language: Английский

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A rapid DNA screening method using high-resolution melt analysis to detect putative Schistosoma haematobium and Schistosoma mattheei hybrids alongside other introgressing schistosomes

Frontiers in Tropical Diseases, Journal Year: 2024, Volume and Issue: 5

Published: Sept. 4, 2024

Background The phenomenon of hybridisation between Schistosoma species has gained a greater degree significance since the WHO declared that schistosomiasis is to be eliminated, as public health problem, by 2030. role plays in transmission disease poorly understood and potential complicate this elimination effort. A primary reason for incomplete understanding schistosome lack suitable, high-throughput easily accessible methods capable identifying species-parentage individual schistosomes. To address resource gap, we present development two-tube HRM assay differentiating schistosomes from possible range six species, namely: S. mattheei, curassoni , bovis, haematobium mansoni margrebowiei. Methods was designed using aligned reference sequences target with primers amplify PCR products species-specific melt temperatures both nuclear mitochondrial genomes. sensitivity specificity these novel primer sets were tested against DNA library comprising representatives of: margrebowiei . optimal annealing temperature real-time (rtPCR) assays established alongside efficiency different pairs. trialled field samples pooled urine school-age children collected 13 schools miracidial preserved on FTA cards. Throughout optimisation testing rtPCR targeting nDNA mtDNA markers comparison pre-published probe-based carried out. Results comparable current, can detect at concentrations 1pg/µL-0.1pg/µL all exception bovis which slightly lower 0.1ng/µL-0.1pg/µL. analysis resulted positive further three rtPCR. identified four addition being mattheei Despite non- filter stored cards only pure Conclusion Although no hybrids detected manuscript novel-two tube described, offers radically increase number screened presence sample types, including biopsy material FGS screening. This will result decrease cost time putative hybrid cases.

Language: Английский

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Genetic profiles of Schistosoma haematobium parasites from Malian transmission hotspot areas

Parasites & Vectors, Journal Year: 2023, Volume and Issue: 16(1)

Published: Aug. 4, 2023

Although schistosomiasis is a public health issue in Mali, little known about the parasite genetic profile. The purpose of this study was to analyze profile schistosomes Schistosoma haematobium group school-aged children various sites Mali.

Language: Английский

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Verification of the Introgression of Narenga porphyrocoma Germplasm into Saccharum officinarum Using Molecular Markers and GISH Analysis

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 121 - 121

Published: Jan. 6, 2025

Sugarcane (Saccharum spp.), a critical crop for sugar and bioenergy production, faces challenges in genetic improvement due to limited diversity from selective breeding. Expanding resources through intergeneric hybridization, particularly with Narenga porphyrocoma, offers promising avenue introduce traits like stress resistance high biomass productivity. However, verifying true hybrids remains challenging traditional morphological methods. This study employed tetra-primer ARMS-PCR genomic situ hybridization (GISH) accurately identify between S. officinarum N. porphyrocoma. Species-specific primers were designed based on SNPs the nrDNA-ITS region ARMS-PCR, enabling effective differentiation of parental hybrid genotypes, while GISH confirmed chromosomal composition hybrids, revealing an n + inheritance pattern. The results demonstrated potential porphyrocoma improve sugarcane’s tillering leaf length, although sucrose content was lower suggesting need further breeding efforts. uniquely contributes sugarcane by providing method verification laying foundation incorporating beneficial genes into cultivars.

Language: Английский

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Imported Schistosomiasis in Southwestern Europe: Wide Variation of Pure and Hybrid Genotypes Infecting Sub‐Saharan Migrants

Transboundary and Emerging Diseases, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Mitochondrial cox 1 and nuclear ribosomal ITSs are commonly combined to distinguish Schistosoma species identify hybrids in endemic countries but very rarely applied patients diagnosed Europe despite the increasing arrival of migrants southwestern Europe. To assess whether those carriers pure or hybrid schistosomes, a complete genetic characterization entering Spain is performed. A total 759 eggs (from urine + stools) from 58 8 African were individually processed describe their mito‐nuclear signature by rapid diagnostic multiplex one‐step polymerase chain reaction (RD‐PCR) ITS‐2/18S sequencing haplotype identification means ITS1‐5.8S‐ITS2 rDNA sequencing. Combined mitochondrial DNA markers sub‐Saharan residing described for first time. Twenty‐two (40.74%) simultaneously carrying urine. S chistosoma haematobium × S. bovis (68.18%) curassoni (31.82%) combinations most frequent. Six (one five hybrid) two (pure) signatures, stools, respectively, 12 61 imported haplotypes found. This study highlights complexity schistosomes that enter Spain, consequently Europe, contributes following: correlate geographical origin with and/or types; detect presence “at distance” (hybrids Guinea‐Bissau Mauritania time detected); molecular pathologies, clinical pictures, treatment responses; and, importantly, warn about possible sources autochthonous transmission.

Language: Английский

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Molecular detection and identification of Trichobilharzia: development of a LAMP, qPCR, and multiplex PCR toolkit

Parasites & Vectors, Journal Year: 2025, Volume and Issue: 18(1)

Published: May 30, 2025

Abstract Background Cercarial dermatitis (CD), or swimmer’s itch, is a water-borne allergic skin reaction caused by the penetration of larval stages bird schistosomes (cercariae) into skin. Members genus Trichobilharzia are primary causative agents CD worldwide. Due to increasing number cases, regarded as (re)emerging disease. Outbreaks in recreational waters can significantly impact public health and local economies. Environmental monitoring crucial for outbreak prediction management. However, conventional methods, such cercarial shedding snail dissections, labour-intensive lack sensitivity. To overcome these limitations, we present molecular toolkit that combines loop-mediated isothermal amplification (LAMP), quantitative polymerase chain (qPCR), multiplex PCR rapid, sensitive, accurate detection identification spp. from various biological samples. Methods Tricho-LAMP Tricho-qPCR were designed optimised DNA detection. A assay was also developed identify three main species causing Europe ( franki , T. szidati regenti ). Results specifically detected at 10 −3 ng, −2 ng per with genomic DNA. Using gBlocks synthetic DNA, achieved 100% 10,000 copies 85% 1000 copies, decreasing success lower concentrations. showed highest sensitivity, detecting all down −4 showing limit reaction. Multiplex allowed reliable differentiation via gel electrophoresis products, but had lowest Conclusions We provide consisting LAMP, qPCR, PCR. By exhibiting high assays potentially suitable environmental (eDNA)-based schistosomes, both researchers authorities. be used determination without need further sequencing. Graphical

Language: Английский

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Genetic characterization of schistosome species from cattle in Côte d’Ivoire

Parasites & Vectors, Journal Year: 2024, Volume and Issue: 17(1)

Published: March 12, 2024

Abstract Background Schistosomiasis is a water-based parasitic disease that affects humans, livestock and wild animals. While considerable resources are dedicated to the surveillance, mapping, control elimination of human schistosomiasis, this not case for schistosomiasis. Indeed, there important data knowledge gaps concerning species present, population genetic diversity, infection prevalence, morbidity economic impact. This study aimed identify circulating schistosome in cattle across Côte d’Ivoire investigate their diversity structuring. Methods Overall, 400 adult schistosomes were collected from slaughtered at six sites d’Ivoire. Additionally, 114 miracidia live one site: Ferkessédougou, northern part DNA all specimens was extracted cox1 ITS1/2 regions amplified analysed confirm species. The structuring populations investigated using 12 microsatellite markers. Results All presented Schistosoma bovis mitochondrial profile. Nuclear ITS1/ 2 obtained 101 four miracidia, which an S. Genetic indices revealed deficiency heterozygotes signals inbreeding sites, while structure analyses displayed little geographic differentiation. Cattle thus appear be mono-species infected with . Hybrids haematobium × have been identified study. panmictic country. Conclusions Our results contribute deeper understanding Ivorian emphasize One Health approach joint animal surveillance prevention programmes Graphical

Language: Английский

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A critical review of the limitations of current diagnostic techniques for schistosomiasis

All Life, Journal Year: 2024, Volume and Issue: 17(1)

Published: July 16, 2024

Schistosomiasis is a parasitic disease that endemic in tropical and subtropical areas. Its diagnosis crucial for effective treatment control, particularly resource-limited settings where the burden high. Various diagnostic methods are available. However, these associated with low accuracy, efficiency or accessibility. This review summarizes published literature on diagnostics of schistosomiasis based techniques microscopy, serology, molecular, antigen-based aptamer-based assays. The limitations each technique were summarized to encourage future research. Furthermore, we highlight need point-of-care sensitive, specific, easy use may address challenges commonly used techniques. concludes further research development needed improve enable control this debilitating disease.

Language: Английский

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Population genetic structure of Schistosoma bovis and S. curassoni collected from cattle in Mali

Parasite, Journal Year: 2024, Volume and Issue: 31, P. 36 - 36

Published: Jan. 1, 2024

Schistosomiasis is of medical and veterinary importance. Despite the critical situation schistosomiasis in sub-Saharan Africa, few molecular epidemiological studies have been carried out to determine role animals its transmission. In Mali, it has over three decades since last study animal schistosomes was out. It now urgent identify circulating strains parasite because potential interactions with other schistosome species, which could complicate disease control. The aim our work composition genetic structure populations collected from cattle. prevalence 23.9%, prevalences Schistosoma bovis (Sb) S. curassoni (Sc) estimated at 12.6% 9.8%, respectively. No hybrid or haematobium were found. parasites displayed distinct geographical distribution Sb dominant Bamako (78.8% 98% Central Slaughterhouse Sabalibougou Slaughterhouses, respectively) Sc Kayes (95.3%). Of 476 a complete profile, 60.4% pure , mainly Kayes. We identified two clusters site level (Fst 0.057 0.042 for respectively). Cluster 1 predominantly composed cluster 2 parasites, Kayes, Our shows that cattle remains endemic Mali . A robust between different identified, included based on parasites.

Language: Английский

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