Digital Lasing Biochip for Tumor-Derived Exosome Analysis DOI Creative Commons
Tian Zhou, Guocheng Fang, Ziyihui Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Digital microfluidics represents an emerging versatile platform that offers numerous advantages in biomolecule detection. However, conventional probes often lack high-intensity and high-sensitivity signals, making it challenging for precise automatic analysis. Recently, optical microresonators stand as a prominent detection the biological field. Here we introduce whispering gallery mode (WGM) microlasers into microwell array, forming digital lasing system. The signal makes highly sensitive, which amplifies subtle changes via strong interactions of light matter. microfluidic droplet technique further allowed with uniform laser thresholds high-throughput fabrication. We utilized this tool analysis exosomes derived from tumor spheroids. believe optofluidic system could serve promising diverse assays various biomedical applications.

Language: Английский

Exosome-mediated delivery and regulation in neurological disease progression DOI Creative Commons
Gurpreet Singh,

Ankit Mehra,

Sanchit Arora

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130728 - 130728

Published: March 11, 2024

Exosomes (EXOs), minute membranous structures originating from diverse biological sources, have recently seized the attention of researchers due to their theranostic potential for neurological diseases. Released actively by various cells, including stem adipose tissue, and immune EXOs wield substantial regulatory influence over intricate landscape complications, exhibiting both positive negative modulatory effects. In AD, play a pivotal role in disseminating breaking down amyloid-β protein. Moreover, derived mesenchymal cells showcase remarkable capacity mitigate pro-inflammatory phenotypes regulating miRNAs neurodegenerative These vesicles possess unique ability traverse blood-brain barrier, governing aggregation mutant huntingtin Understanding exosomal functions within CNS holds significant promise enhancing treatment efficacy This review intricately examines mechanisms involving disease development, highlighting therapeutic prospects exploring utility exosome-based nanomedicine complications. Additionally, delves into challenges associated with drug delivery brain, emphasizing complexities inherent this critical aspect neurotherapeutics.

Language: Английский

Citations

38
Exosomes: a promising avenue for cancer diagnosis beyond treatment DOI Creative Commons
Zhu Wang, Qianqian Wang, Feng Qin

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Feb. 13, 2024

Exosomes, extracellular vesicles secreted by cells, have garnered significant attention in recent years for their remarkable therapeutic potential. These nanoscale carriers can be harnessed the targeted delivery of agents, such as pharmaceuticals, proteins, and nucleic acids, across biological barriers. This versatile attribute exosomes is a promising modality precision medicine applications, notably realm cancer therapy. However, despite substantial potential, still confront challenges tied to standardization scalability that impede practice clinical applications. Moreover, heterogeneity isolation methodologies limited cargo loading mechanisms pose obstacles ensuring consistent outcomes, thereby constraining utility. In contrast, exhibit distinct advantage diagnosis, they harbor specific signatures reflective tumor's genetic proteomic profile. characteristic endows them with potential serve valuable liquid biopsies non-invasive real-time monitoring, making possible early detection development personalized treatment strategies. this review, we provide an extensive evaluation advancements exosome research, critically examining advantages limitations context therapy diagnosis. Furthermore, present curated overview most technological innovations utilizing exosomes, focus on enhancing efficacy detection.

Language: Английский

Citations

33
Nanoparticle-Based Combinational Strategies for Overcoming the Blood-Brain Barrier and Blood-Tumor Barrier DOI Creative Commons
Su Hyun Lim, Gi Taek Yee, Dongwoo Khang

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 2529 - 2552

Published: March 1, 2024

Abstract: The blood-brain barrier (BBB) and blood-tumor (BTB) pose substantial challenges to efficacious drug delivery for glioblastoma multiforme (GBM), a primary brain tumor with poor prognosis. Nanoparticle-based combinational strategies have emerged as promising modalities overcome these barriers enhance penetration into the parenchyma. This review discusses various nanoparticle-based combinatorial approaches that combine nanoparticles cell-based delivery, viral focused ultrasound, magnetic field, intranasal permeability across BBB BTB. Cell-based involves using engineered cells carriers nanoparticles, taking advantage of their intrinsic migratory homing capabilities facilitate transport therapeutic payloads Viral uses vectors deliver genes or specific within GBM microenvironment. Focused coupled microbubbles can temporarily disrupt increase permeability. Magnetic field-guided exploits targeted under an external field. Intranasal offers minimally invasive avenue bypass agents directly via olfactory trigeminal pathways. By combining strategies, synergistic effects efficiency, improve efficacy, reduce off-target effects. Future research should focus on optimizing nanoparticle design, exploring new combination advancing preclinical clinical investigations promote translation therapies GBM. Keywords: glioblastoma, barrier, nanoparticle, strategy, ultrasound-wave,

Language: Английский

Citations

17
Exosome Source Matters: A Comprehensive Review from the Perspective of Diverse Cellular Origins DOI Creative Commons
Y Chen, Weili Qi, Zhenghao Wang

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(2), P. 147 - 147

Published: Jan. 22, 2025

Exosomes have emerged as promising therapeutic agents in regenerative medicine. This review introduces a novel cell type-oriented perspective to systematically analyze exosomal properties therapies. To our knowledge, this is the first comprehensively compare exosomes based on cellular source type, offering unprecedented insights into selecting optimal exosome producers for targeted applications. Factors beyond origin influencing efficacy, such donor sites and collection methods, are also explored here. By synthesizing key advances, we propose research directions end. We aim accelerate development of more effective exosome-based therapies highlight underexplored rapidly evolving field.

Language: Английский

Citations

2
Breaking barriers in targeted Therapy: Advancing exosome Isolation, Engineering, and imaging DOI
Anastasiya Kostyusheva, Eugenia Romano, Neng Yan

et al.

Advanced Drug Delivery Reviews, Journal Year: 2025, Volume and Issue: 218, P. 115522 - 115522

Published: Jan. 22, 2025

Language: Английский

Citations

2
Resveratrol-Enhanced Human Neural Stem Cell-Derived Exosomes Mitigate MPP+-Induced Neurotoxicity Through Activation of AMPK and Nrf2 Pathways and Inhibition of the NLRP3 Inflammasome in SH-SY5Y Cells DOI Creative Commons
Tony F. Chan, Yuping Yang, Christopher J.B. Nicol

et al.

Life, Journal Year: 2025, Volume and Issue: 15(2), P. 294 - 294

Published: Feb. 13, 2025

Parkinson's disease (PD) is a progressive neurodegenerative disorder primarily characterized by the loss of dopaminergic neurons in substantia nigra. Mitochondrial dysfunction, oxidative stress, and neuroinflammation are recognized as critical pathological mechanisms driving neurodegeneration PD. Exosome (Exo)-based therapies, particularly those derived from human neural stem cells (hNSCs), offer promising neuroprotective effects due to their ability transfer bioactive molecules that modulate cellular processes. Resveratrol (RES), polyphenolic compound with potent antioxidant anti-inflammatory properties, has been shown enhance therapeutic potential cell (SC)-derived Exos. This study investigated RES-treated hNSCs-derived Exos (RES-hNSCs-Exos) on SH-SY5Y exposed 1-methyl-4-phenylpyridinium (MPP+), neurotoxin commonly used model Parkinsonian neurotoxicity. Treating MPP+ led significant reductions viability, mitochondrial increased activation inflammatory pathways. Treatment RES-hNSCs-Exos rescued MPP+-induced toxicity improving enhancing ATP production, increasing biogenesis, reducing reactive oxygen species (ROS) generation. The findings also demonstrated expression essential genes involved such PGC1α, NRF1, Tfam, indicating improved function presence RES-hNSCs-Exos. Further analysis revealed these protective were mediated activating AMP-activated protein kinase (AMPK) Nrf2 signaling pathways, which promoted health reduced stress. Moreover, treatment suppressed downregulating NLRP3 inflammasome secretion pro-inflammatory cytokines IL-1β IL-18. In conclusion, results suggest exhibit against neurotoxicity function, inhibiting neuroinflammation. These highlight hNSCs-Exos novel strategy for diseases like PD, RES valuable enhancer efficacy.

Language: Английский

Citations

2
3D printing of interferon γ-preconditioned NSC-derived exosomes/collagen/chitosan biological scaffolds for neurological recovery after TBI DOI Creative Commons
Chong Chen,

Zhe‐Han Chang,

Bin Yao

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 39, P. 375 - 391

Published: May 28, 2024

The reconstruction of neural function and recovery chronic damage following traumatic brain injury (TBI) remain significant clinical challenges. Exosomes derived from stem cells (NSCs) offer various benefits in TBI treatment. Numerous studies confirmed that appropriate preconditioning methods enhanced the targeted efficacy exosome therapy. Interferon-gamma (IFN-γ) possesses immunomodulatory capabilities is widely involved neurological disorders. In this study, IFN-γ was employed for NSCs to enhance (IFN-Exo, IE) TBI. miRNA sequencing revealed potential IFN-Exo promoting differentiation modulating inflammatory responses. Through low-temperature 3D printing, combined with collagen/chitosan (3D-CC-IE) preserve biological activity exosome. delivery exosomes via biomaterial scaffolds benefited retention therapeutic exosomes, ensuring they could exert long-term effects at site. 3D-CC-IE scaffold exhibited excellent biocompatibility mechanical properties. Subsequently, significantly improved impaired motor cognitive functions after rat. Histological results showed markedly facilitated damaged tissue promoted endogenous neurogenesis. Further mechanistic validation suggested alleviated neuroinflammation by MAPK/mTOR signaling pathway. summary, study indicated engaged pathophysiological processes, fostering TBI, offering a promising regenerative therapy avenue.

Language: Английский

Citations

9
Clinical Management in Traumatic Brain Injury DOI Creative Commons

Amy Yan,

Andrew Torpey,

Erin Morrisroe

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(4), P. 781 - 781

Published: April 2, 2024

Traumatic brain injury is one of the leading causes morbidity and mortality worldwide major public healthcare burdens in US, with millions patients suffering from traumatic itself (approximately 1.6 million/year) or its repercussions (2–6 million disabilities). The severity can range mild transient neurological dysfunction impairment to severe profound disability that leaves completely non-functional. Indications for treatment differ based on injury’s severity, but goals early prevent secondary injury. Hemodynamic stability, monitoring intracranial pressure, maintenance cerebral perfusion support adequate oxygenation ventilation, administration hyperosmolar agents and/or sedatives, nutritional support, seizure prophylaxis are mainstays medical Surgical management options include decompressive craniectomy cerebrospinal fluid drainage via insertion an external ventricular drain. Several emerging modalities being investigated, such as anti-excitotoxic agents, anti-ischemic dysregulation S100B protein, erythropoietin, endogenous neuroprotectors, anti-inflammatory stem cell neuronal restoration among others.

Language: Английский

Citations

7
Conditioned medium-enriched umbilical cord mesenchymal stem cells: a potential therapeutic strategy for spinal cord injury, unveiling transcriptomic and secretomic insights DOI
Rajasekaran Subbarayan, Murugan Dinesh, S. Thirupathi Kumara Raja

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: April 24, 2024

Language: Английский

Citations

7
Exosomes: Traversing the blood-brain barrier and their therapeutic potential in brain cancer DOI
Xiaopei Zhang,

Nichole Artz,

Dennis A. Steindler

et al.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2025, Volume and Issue: unknown, P. 189300 - 189300

Published: March 1, 2025

Language: Английский

Citations

1