CLINICAL EFFECTIVENESS AND FEATURES OF THE POSTOPERATIVE PERIOD IN PATIENTS AFTER OSTEOSYNTHESIS OF THE MANDIBLE USING CELL TECHNOLOGIES DOI Creative Commons
А. В. Бамбуляк, N. B. Kuzniak, L.Ya. Lopushniak

et al.

Neonatology surgery and perinatal medicine, Journal Year: 2024, Volume and Issue: 14(3(53)), P. 99 - 107

Published: Nov. 13, 2024

Due to a signifi cant increase in the frequency and complexity of maxillofacial injuries, one priorities development modern surgical dentistry is search for new eff ective methods treating facial bone fractures. In recent decades, attention clinicians has been focused on biological treatment aimed at restoring defects using cell technologies transplants.The aim study was determine ect osteoplastic materials based multipotent adipose- derived mesenchymal stromal cells dental patients mandibular fractures.Materials methods. The clinical material included 56 patients, 13 whom underwent osteosynthesis with hydroxyapatite graft substitute (subgroup 1I), 25 surgery combination adipose tissue cells, platelet-rich plasma 1B), 18 spontaneous healing fracture line 1C). Patients were clinically evaluated daily basis. Clinical parameters assessed days 1, 3, 5, 7, 10, 14 28 after surgery. postoperative pain syndrome Numerical Rating Scale (NRS), considering subjective signs operated patients.The statistical processing results performed analysis software biomedical research ‘Microsoft Excel 21’ ‘Statistica’ (licence number STA862D175437Q). results, we used variational series – calculation arithmetic mean its standard error (Mim) cance diff erences obtained comparison groups Student’s t-test method. p<0.05 considered erence. [17]. conducted accordance basic provisions «Rules Ethical Principles Scientifi c Medical Research Involving Human Subjects» approved by Declaration Helsinki (1964-2013), ICH GCP (1996), EEC Directive 609 (24.11.1986), Orders Ministry Health Ukraine ¹ 690 23.09.2009, 944 14.12.2009, 616 03.08.2012. Commission Biomedical Ethics Bukovinian State University found no violations ethical moral standards conduct (ProtocolNo. 6, 16.03.2023). signed informed consent participate this all measures taken ensure their anonymity.The results. comparative ectiveness use various performance lower law confi rmed advantages before augmentation. It data objective symptoms during period.Conclusions. Our show that tissue, blood promising material. Its application improves regenerative reparative properties facilitates period shortens duration hospitalization, promotes innovative ways reconstructive osteogenesis

Language: Английский

Adipose-Derived Stem Cell Exosomes for Stress Urinary Incontinence: A Novel in Vivo Therapeutic Approach DOI

Basma Hamed,

Mohamed L. Salem,

Esam Mosbah

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract This study aimed to evaluate the therapeutic effects of Adipose tissue-derived stem cell exosomes (ADSCE) in management stress urinary incontinence (SUI) using vaginal dilatation-induced acute and chronic SUI models Sprague Dawley rats. Flow cytometry confirmed mesenchymal identity isolated UC-SCs, while exosomal markers (CD9, CD63, CD81) validated successful exosome isolation. Transmission electron microscopy revealed characteristic nanoscale morphology exosomes, further supported by energy-dispersive X-ray analysis. Therapeutic efficacy was assessed through urodynamic histopathological analyses. Abdominal leak point pressure (ALPP) significantly reduced both compared controls (P < 0.0001). ADSCE therapy increased ALPP, with systemic administration demonstrating superior over local treatment 0.05). Histopathological examination indicated substantial sphincter muscle thinning, edema, fibrosis untreated models, mitigated these pathological changes. Masson’s Trichrome staining significant preservation urethral thickness treated groups 0.01), yielding moderate improvements administration. Furthermore, markedly collagen deposition, particularly groups, suggesting an antifibrotic effect enhanced tissue remodeling. These findings indicate that effectively restores function mitigates alterations SUI. Systemic demonstrates potential, highlighting as a promising regenerative strategy for management.

Language: Английский

Citations

0
Tendon extracellular-matrix-derived tissue engineering micro-tissue for Achilles tendon injury regeneration in rats DOI Creative Commons
Kaihong Zhang,

Peng Zhang,

Ge Shi

et al.

Journal of Orthopaedic Surgery and Research, Journal Year: 2024, Volume and Issue: 19(1)

Published: June 27, 2024

Abstract Background Achilles tendon is vital in maintaining the stability and function of ankle joint. It quite difficult to achieve structural functional repair tissue engineering. Methods A tissue-engineered micro-tissue was prepared using rat tail extracellular matrix (TECM) combined with adipose stem cells (ADSCs) injuries. The TECM by repeated freezing thawing. vitro characteristics its effect on ADSCs proliferation were detected. This for vivo evaluated based general characteristics, gait analysis, ultrasound findings, histological biomechanical testing. Results results showed that scaffold had good biocompatibility ADSCs. At 2 weeks post-surgery, collagen types I III tenomodulin expression higher, vascular endothelial growth factor lower group than other groups. 4 8 analysis findings repaired smooth lustrous, arranged regularly evenly group. Gait confirmed better motor recovery noted In addition, mechanical properties Conclusion Tissue-engineered fabricated has can promote vivo. composite biomaterial broad application prospects

Language: Английский

Citations

1
Cell-free adipose tissue extracts as a novel treatment for rosacea by downregulating TRPV1 DOI Creative Commons

Liuyi Zhou,

Lulu Chen, Ting Li

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 18, 2024

Language: Английский

Citations

1
Small Extracellular Vesicles by Nucleus Pulposus Cells Maintain Niche and Cell Homeostasis via Receptor Shuffling and Metabolic Enzyme Supplements DOI Creative Commons

Ankita Samanta,

Mi‐Jeong Yoo, Jin Koh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 14, 2024

Background: Small extracellular vesicles (sEV) are a conserved mean of communication across the domains life and lately gained more interest in mammalian non-cancerous work as non-cellular, biological therapeutic with encouraging results recent studies chronic degenerative diseases. The nucleus pulposus (NP) is avascular aneural center an intervertebral disc (IVD), home to unique niche conditions affected IVD degeneration. We investigated proteome autologous mesenchymal stem cell (MSC) sEVs for their potential contribute tissue homeostasis this niche. Methods: aimed identify characteristics NP via mass spectrometric functional enrichment analysis. explored profile generated by parent lines from bovine coccygeal adipose tissue, focus on cells, using adult fetal donors. compared these findings published sEV databases MSC data. Findings: propose several mechanisms associated sEVs: 1. Membrane receptor trafficking modify signal responses promoting homeostasis. 2. Cell proteasome delivery; 3. Redox energy metabolic enzymes delivery. 4. Immunomodulation beyond association serum protein corona. Conclusion: signature cells similar previously data, yet supplementing anaerobic metabolism redox balance while contributing maintenance microniche.

Language: Английский

Citations

1
Mechanobiological Strategies to Enhance Ovine (Ovis aries) Adipose-Derived Stem Cells Tendon Plasticity for Regenerative Medicine and Tissue Engineering Applications DOI Creative Commons
Arlette A. Haidar-Montes, Annunziata Mauro, Mohammad El Khatib

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(15), P. 2233 - 2233

Published: July 31, 2024

Adipose-derived stem cells (ADSCs) hold promise for tendon repair, even if their tenogenic plasticity and underlying mechanisms remain only partially understood, particularly in derived from the ovine animal model. This study aimed to characterize oADSCs during vitro expansion validate phenotypic properties pre-transplantation. Moreover, potential was assessed using two vitro-validated approaches: (1) teno-inductive conditioned media (CM) a co-culture between amniotic fetal explants, (2) short- (48 h) long-term (14 days) seeding on highly aligned PLGA (ha-PLGA) electrospun scaffold. Our findings indicate that can be expanded without senescence maintain expression of stemness (Sox2, Oct4, Nanog) mesenchymal (CD29, CD166, CD44, CD90) markers while remaining negative hematopoietic (CD31, CD45) MHC-II antigens. Of note, oADSCs' differentiation greatly depended strategy. exposed CM significantly upregulated tendon-related genes (COL1, TNMD, THBS4) but failed accumulate TNMD protein at 14 days culture. Conversely, seeded ha-PLGA fleeces quickly accumulated high levels into cytoplasm ADSCs, displaying tenocyte-like morphology. mechano-sensing cellular response involved complete SOX9 downregulation accompanied by YAP activation, highlighting efficacy biophysical stimuli promoting differentiation. These underscore self-renewal differentiative potential, thus opening use preclinical setting develop innovative cell-based tissue engineering protocols regeneration, applied veterinary field.

Language: Английский

Citations

0
Protective function of adipocyte-derived extracellular vesicles and adipose stem cells in damage repair and regeneration DOI Creative Commons
Yanwen Wang, Poh‐Ching Tan, Xuewen Xu

et al.

Chinese Journal of Plastic and Reconstructive Surgery, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

0
Promoting Radial Repair in Rabbits through BMP-9 Overexpression in Adipose-Derived Stem Cells Loaded with ICA/nHAC/PLA DOI Creative Commons

Fang-Tian Xu,

Lin Wang,

Shuang-Yi Li

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Abstract Background Bone defects commonly cause delayed bone healing and local dysfunction. Both adipose-derived stem cells (ADSCs) icariin (ICA)-loaded nano-hydroxyapatite/collagen/polylactic acid (nHAC/PLA) have been reported to promote osteoblast metabolism accelerate fracture healing. Aim The aim of this study was explore the mechanism ADSCs combined with ICA-loaded nHAC/PLA in regulating defects. Methods effects ICA BMP-9 (Bone morphogenetic protein-9) overexpression on cell viability osteogenic differentiation were measured by qRT-PCR, western blotting alkaline phosphatase (ALP) assay. Alizarin red staining examined formation mineralized nodules ADSCs. Finally, radial defect rabbit model established, administrated nHAC/PLA. new detected X-ray, immunohistochemistry Hematoxylin eosin staining. Results significantly increased ALP activity nodule ADSCs, that amplified ICA. This combination also upregulated expression markers (BMP-9, OCN, OPN, COL-1, BSP, Runx-2) influenced key signaling pathways (p38 MAPK, JNK, ERK). In vivo, treatment group showed enhanced repair. Conclusions Our findings indicate BMP-9-overexpressed scaffolds is effective promoting repair, offering a potential therapeutic strategy for Core tip promoted proliferation ADSCs. BMP-9 osteogenesis ADSCs

Language: Английский

Citations

0
CLINICAL EFFECTIVENESS AND FEATURES OF THE POSTOPERATIVE PERIOD IN PATIENTS AFTER OSTEOSYNTHESIS OF THE MANDIBLE USING CELL TECHNOLOGIES DOI Creative Commons
А. В. Бамбуляк, N. B. Kuzniak, L.Ya. Lopushniak

et al.

Neonatology surgery and perinatal medicine, Journal Year: 2024, Volume and Issue: 14(3(53)), P. 99 - 107

Published: Nov. 13, 2024

Due to a signifi cant increase in the frequency and complexity of maxillofacial injuries, one priorities development modern surgical dentistry is search for new eff ective methods treating facial bone fractures. In recent decades, attention clinicians has been focused on biological treatment aimed at restoring defects using cell technologies transplants.The aim study was determine ect osteoplastic materials based multipotent adipose- derived mesenchymal stromal cells dental patients mandibular fractures.Materials methods. The clinical material included 56 patients, 13 whom underwent osteosynthesis with hydroxyapatite graft substitute (subgroup 1I), 25 surgery combination adipose tissue cells, platelet-rich plasma 1B), 18 spontaneous healing fracture line 1C). Patients were clinically evaluated daily basis. Clinical parameters assessed days 1, 3, 5, 7, 10, 14 28 after surgery. postoperative pain syndrome Numerical Rating Scale (NRS), considering subjective signs operated patients.The statistical processing results performed analysis software biomedical research ‘Microsoft Excel 21’ ‘Statistica’ (licence number STA862D175437Q). results, we used variational series – calculation arithmetic mean its standard error (Mim) cance diff erences obtained comparison groups Student’s t-test method. p<0.05 considered erence. [17]. conducted accordance basic provisions «Rules Ethical Principles Scientifi c Medical Research Involving Human Subjects» approved by Declaration Helsinki (1964-2013), ICH GCP (1996), EEC Directive 609 (24.11.1986), Orders Ministry Health Ukraine ¹ 690 23.09.2009, 944 14.12.2009, 616 03.08.2012. Commission Biomedical Ethics Bukovinian State University found no violations ethical moral standards conduct (ProtocolNo. 6, 16.03.2023). signed informed consent participate this all measures taken ensure their anonymity.The results. comparative ectiveness use various performance lower law confi rmed advantages before augmentation. It data objective symptoms during period.Conclusions. Our show that tissue, blood promising material. Its application improves regenerative reparative properties facilitates period shortens duration hospitalization, promotes innovative ways reconstructive osteogenesis

Language: Английский

Citations

0