Therapeutic potential of human mesenchymal stromal cell-derived mitochondria in a rat model of post-surgical digestive fistula: towards an energetic nano-biotherapy DOI Open Access
Antoine Mariani, A Guichard, Anna Sebbagh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Abstract Background Tissue regeneration heavily relies on cellular energy production, with mitochondria playing a crucial role. Dysfunctional are implicated in various degenerative diseases, driving interest targeting mitochondrial transplantation for tissue repair. Wound healing is highly compromised gastrointestinal conditions resulting fistula development, particularly after sleeve gastrectomy. Human mesenchymal stem/stromal cells (hMSCs) and their cell-free products such as offer potential benefits due to therapeutic properties production. Here we investigated the advantage of hMSCs-derived nano-biotherapy rat model post-surgical healing. Methods Viable structurally intact were isolated from hMSCs before exposure human colonic epithelial (HCEC-1CT) culture or into post-operative fistula. Results Our findings reveal significant dose-dependent improvement metabolic activity ATP content recipient cells. Assessment external orifice developed following post gastrectomy fistula, revealed substantial all transplanted rats compared control group. Conclusion highlight This research contributes advancing regenerative strategies conditions, offering new insights mitochondrial-based therapies enhancing wound

Language: Английский

Stroke Studies in Large Animals: Prospects of Mitochondrial Transplantation and Enhancing Efficiency using Hydrogels and Nanoparticle-assisted Delivery DOI
Öner Ülger, Ismail Eş, Christopher M. Proctor

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 100, P. 102469 - 102469

Published: Aug. 25, 2024

Language: Английский

Citations

3

Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model DOI Creative Commons

Hui Bang Cho,

Hye‐Ryoung Kim,

Sujeong Lee

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract Mitochondrial transfer emerges as a promising therapy for the restoration of mitochondrial function in damaged cells, mainly due to its limited immunogenicity. However, isolated mitochondria rapidly lose because they produce little energy outside cells. Therefore, this study investigates whether near infrared (NIR)‐mediated nicotinamide adenine dinucleotide (NADH) pre‐treatment enhances and stability mitochondria‐donor cells prior transplantation. Clinical applications NADH, an essential electron donor oxidative phosphorylation process, are restricted cellular uptake NADH. To address this, photo‐mediated method optimizes direct NADH delivery into increases absorption. L6 treated with irradiated NIR enhanced uptake, significantly improving production function. Importantly, improved functional characteristics maintained even after isolation from Primed mitochondria, i.e., those by NIR‐mediated (P‐MT), encapsulated fusogenic liposomes delivered muscle dysfunction. Compared conventional P‐MT promote greater recovery regeneration. These findings suggest that is effective strategy function, has potential lead novel treatments disorders degeneration.

Language: Английский

Citations

0

Organoids as 3D Models for Studying Exogenous Mitochondrial Transplantation DOI
Ismail Eş, Öner Ülger

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0

Mitochondrial-based therapies for neurodegenerative diseases: a review of the current literature DOI Creative Commons

Al-Hassan Soliman Wadan,

Ahmed Shaaban, Mohamed El‐Sadek

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Abstract Neurodegenerative disorders present significant challenges to modern medicine because of their complex etiology, pathogenesis, and progressive nature, which complicate practical treatment approaches. Mitochondrial dysfunction is an important contributor the pathophysiology various neurodegenerative illnesses, including Alzheimer’s disease (AD), Parkinson’s (PD), amyotrophic lateral sclerosis (ALS). This review paper examines current literature highlighting multifaceted functions mitochondria, energy production, calcium signaling, apoptosis regulation, mitochondrial biogenesis, dynamics, axonal transport, endoplasmic reticulum–mitochondrial interactions, mitophagy, proteostasis, crucial involvement in neuronal health. The emphasizes increasing recognition as a critical factor progression disorders, marking shift from traditional symptom management innovative mitochondrial-based therapies. By discussing mechanisms, quality control (MQC) processes impact oxidative stress, this highlights need for novel therapeutic strategies restore function, protect connections integrity, slow progression. comprehensive aims provide insights into potential interventions that could transform landscape diseases, addressing symptoms underlying pathophysiological changes.

Language: Английский

Citations

0

Mitochondria for Cardiovascular Therapy: A Deep Dive into Drug Targets and Therapeutic Approaches DOI
Martina Semenzato, Déborah Naón

Current Pharmacology Reports, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 4, 2025

Language: Английский

Citations

0

Future Directions in Reactive Oxygen Species Research DOI
Suyash Saxena, Rahul Saxena, Ajit Pal Singh

et al.

Advances in medical diagnosis, treatment, and care (AMDTC) book series, Journal Year: 2025, Volume and Issue: unknown, P. 507 - 528

Published: Jan. 3, 2025

Reactive oxygen species (ROS) are crucial for immune defense and cellular signaling, but their overproduction can damage cells contribute to diseases. Understanding this duality is essential developing ROS-based therapies. Future treatments might target specific ROS types or production mechanisms, offering promise conditions like neurodegeneration, cardiovascular diseases, certain cancers. Controlled generation could enable targeted cancer cell killing signaling pathway activation. biomarkers aid in early disease diagnosis treatment monitoring. However, challenges include the complexity of biology, lack sensitive detection tools, difficulties translating research clinical settings. Advanced techniques precise measurement visualization needed, along with ethical considerations ensure safety efficacy. Collaborative efforts vital unlock potential novel therapeutic approaches.

Language: Английский

Citations

0

Mitochondrial transfer in endothelial cells and vascular health DOI
Gwang‐Bum Im, Juan M. Melero‐Martin

Trends in Cell Biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0

Mitochondrial and photosynthetic therapy: A crucial strategy for remodeling cellular metabolic function DOI Creative Commons
Muhammad Samee Mubarik, Zizhen Zhao, Mehdi Khoshnamvand

et al.

Bioengineering & Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

Abstract Extranuclear organelle transplantation, an emerging field in cell biology and bioengineering, presents innovative therapeutic possibilities by transferring organelles such as mitochondria between cells or across species. In living organisms, chloroplasts are closely related to converting substances energy within cells. Transplantation therapy of seeks rebuild metabolic function diseased damaged has broad application potential treating diseases. The therapies provide a distinctive technology for cellular restoration targeting generation at the level, which will offer new resources animal At present, mitochondrial transplantation been applied novel approach rescue patients clinical settings, chloroplast‐based endows utilize light (photosynthetic cells). this review, we discuss exciting development prospects photosynthetic biomedicine. extranuclear would exert profound impacts on biological therapy.

Language: Английский

Citations

0

Therapeutic potential of human mesenchymal stromal cell-derived mitochondria in a rat model of post-surgical digestive fistula: towards an energetic nano-biotherapy DOI Open Access
Antoine Mariani, A Guichard, Anna Sebbagh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Abstract Background Tissue regeneration heavily relies on cellular energy production, with mitochondria playing a crucial role. Dysfunctional are implicated in various degenerative diseases, driving interest targeting mitochondrial transplantation for tissue repair. Wound healing is highly compromised gastrointestinal conditions resulting fistula development, particularly after sleeve gastrectomy. Human mesenchymal stem/stromal cells (hMSCs) and their cell-free products such as offer potential benefits due to therapeutic properties production. Here we investigated the advantage of hMSCs-derived nano-biotherapy rat model post-surgical healing. Methods Viable structurally intact were isolated from hMSCs before exposure human colonic epithelial (HCEC-1CT) culture or into post-operative fistula. Results Our findings reveal significant dose-dependent improvement metabolic activity ATP content recipient cells. Assessment external orifice developed following post gastrectomy fistula, revealed substantial all transplanted rats compared control group. Conclusion highlight This research contributes advancing regenerative strategies conditions, offering new insights mitochondrial-based therapies enhancing wound

Language: Английский

Citations

0