Highlights on Future Treatments of IPF: Clues and Pitfalls

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8392 - 8392

Published: Aug. 1, 2024

Idiopathic pulmonary fibrosis (IPF) is an interstitial lung disease characterized by irreversible scarring of tissue, leading to death. Despite recent advancements in understanding its pathophysiology, IPF remains elusive, and therapeutic options are limited non-curative. This review aims synthesize the latest research developments, focusing on molecular mechanisms driving related emerging treatments. Unfortunately, several phase 2 studies showing promising preliminary results did not meet primary endpoints subsequent 3, underlying complexity need for new integrated endpoints. a challenging condition with complex interplay genetic, epigenetic, pathophysiological factors. Ongoing into keystones critical development targeted therapies that could potentially stop progression disease. Future directions include personalized medicine approaches, artificial intelligence integration, growth genetic insights, novel drug targets.

Language: Английский

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hAMSCs regulate EMT in the progression of experimental pulmonary fibrosis through delivering miR-181a-5p targeting TGFBR1

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 5, 2025

Pulmonary fibrosis (PF) is a common and multidimensional devastating interstitial lung disease. The development of novel more effective interventions for PF an urgent clinical need. A previous study has found that miR-181a-5p plays important role in the PF, human amniotic mesenchymal stem cells (hAMSCs) exert potent therapeutic potential on PF. However, whether hAMSCs act by delivering its detailed mechanism still remain unknown. Thus, this was designed to investigate underlying possible bleomycin (BLM)-induced mouse model, co-culture system A549 epithelial transition (EMT) focusing effects collagen deposition, EMT, cell cycle regulation. with different expression levels were constructed. BLM (4 mg/kg) used create while TGF-β1 induce construct EMT model. Furthermore, deposition during assessed vivo vitro. We exerted anti-fibrotic effect BLM-induced Moreover, also protective TGFβ1-induced ameliorated promoting proliferation, reducing apoptosis, attenuating through paracrine effects. regulated targeting TGFBR1. Our findings reveal first time inhibit EMT. Mechanistically, hMASCs achieved

Language: Английский

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Characterization of rectus femoris lesions in knee osteoarthritis at different stages and the effect of ultrasound-guided acupotomy

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 23, 2025

Introduction Chronic injury to the rectus femoris muscle induces and exacerbates progression of knee osteoarthritis (KOA). However, lesion characteristics in KOA at different stages have not been fully characterized. The aim this study was analyze pattern investigate mechanism by which ultrasound-guided acupotomy operations can prevent control KOA. Methods Early, middle, late-stage rabbit models were constructed using modified Videman method. Ultrasonography used record elastic modulus cross-sectional area muscle, morphology observe ultramicroscopic changes assess degree fibrosis. Additionally, performed on model KOA, alterations key molecular markers fibrosis determined Western Blot qPCR methods. Results As disease progressed, rabbits gradually increased, decreased, increased. In contrast, improved after intervention. Conclusion These findings highlight gradual increase elasticity, decrease area, increased as progressed. Ultrasoundguided shown a protective effect cartilage delay ameliorating pathological muscle. may involve reducing chronic protecting joint homeostasis attenuating

Language: Английский

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SVF-gel application for the alleviation of full-thickness skin graft contraction: an experimental study in mice

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 29, 2025

Skin grafts often suffer from contracture, complicating recovery. SVF-gel shows promise in addressing scar but its therapeutic effects and mechanisms are not fully understood. This study evaluates the efficacy of full-thickness skin grafting. Full-thickness were harvested mice dorsal skin, rotated sutured. or saline was injected beneath muscle fascia. Immunohistochemistry assessed SVF-gel's on angiogenesis, collagen deposition, fibrosis, dermal adipocytes. Keloid-related genes GSE92566 GSE158395 analyzed for functional enrichment protein-protein interactions, with hub validated using GSE190626. significantly increased grafted area, thickness epidermal adipose layers, hair follicles compared to control group. enhanced CD31 perilipin expression, decreased α-SMA identified HLA+ cells around CD31+ microvessels tissue graft. Sixty commonly downregulated keloid-related identified, KEGG pathway analysis indicating PPAR signaling lipolysis regulation. Five (ADIPOQ, FABP4, KRT7, LEP, PIP) validated. as a stem cell therapy grafts, improving outcomes by enhancing revascularization, increasing fiber density regularity, accelerating myofibroblast turnover, promoting adipogenesis, follicles.

Language: Английский

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TGFβ family signaling in human stem cell self-renewal and differentiation

Cell Regeneration, Journal Year: 2024, Volume and Issue: 13(1)

Published: Nov. 28, 2024

Abstract Human stem cells are undifferentiated with the capacity for self-renewal and differentiation into distinct cell lineages, playing important role in development maintenance of diverse tissues organs. The microenvironment provides crucial factors components that exert significant influence over determination fate. Among these factors, cytokines from transforming growth factor β (TGFβ) superfamily, including TGFβ, bone morphogenic protein (BMP), Activin Nodal, have been identified as regulators governing differentiation. In this review, we present a comprehensive overview pivotal roles played by TGFβ superfamily signaling human embryonic cells, somatic induced pluripotent cancer cells. Furthermore, summarize latest research advancements family various cell-based therapy, discussing their potential clinical applications therapy regeneration medicine.

Language: Английский

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Invited Review for 20th Anniversary Special Issue of PLRev “AI for Mechanomedicine”

Physics of Life Reviews, Journal Year: 2024, Volume and Issue: 51, P. 328 - 342

Published: Oct. 24, 2024

Language: Английский

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