Mouse spontaneous behavior reflects individual variation rather than estrous state

Current Biology, Journal Year: 2023, Volume and Issue: 33(7), P. 1358 - 1364.e4

Published: March 7, 2023

Behavior is shaped by both the internal state of an animal and its individual behavioral biases. Rhythmic variation in gonadal hormones during estrous cycle a defining feature female state, one that regulates many aspects sociosexual behavior. However, it remains unclear whether influences spontaneous behavior and, if so, how these effects might relate to variation. Here, we address this question longitudinally characterizing open-field mice across different phases cycle, using unsupervised machine learning decompose into constituent elements.

Language: Английский

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Minimal influence of estrous cycle on studies of female mouse behaviors

Frontiers in Molecular Neuroscience, Journal Year: 2023, Volume and Issue: 16

Published: July 4, 2023

Sex bias has been an issue in many biomedical fields, especially neuroscience. In rodent research, scientists only focused on male animals due to the belief that female estrous cycle gives rise unacceptable, high levels of variance experiments. However, even though sexual behaviors are well known be regulated by cycle, which effects other non-sexual were not always consistent previous reports. Recent reviews analyzing published literature suggested there is no evidence for larger variation than several phenotypes.To further investigate impact variability behaviors, we conducted multiple behavioral assays, including open field test, forced swimming and resident-intruder assay assess anxiety-, depression-like as social interaction respectively. We compared females estrus diestrus stages across four different mouse strains: C57BL/6, BALB/c, C3H, DBA/2.Our results found significant difference most parameters between these two stages. On hand, differences among certain strains relatively both stages, suggesting a very minimal effect detecting difference. Last, similar variations sexes.While our study successfully identified sexes, did find solid support notion influenced cycle. Additionally, observed males females. Female mice, therefore, have reason excluded future research.

Language: Английский

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New perspectives on sex differences in learning and memory

Trends in Endocrinology and Metabolism, Journal Year: 2023, Volume and Issue: 34(9), P. 526 - 538

Published: July 25, 2023

Language: Английский

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Epigenetic mechanisms underlying sex differences in the brain and behavior

Trends in Neurosciences, Journal Year: 2023, Volume and Issue: 47(1), P. 18 - 35

Published: Nov. 15, 2023

Sex differences are found across brain regions, behaviors, and diseases. Sexual differentiation of the is initiated prenatally but it continues throughout life, as a result interaction three major factors: gonadal hormones, sex chromosomes, environment. These factors thought to act, in part, via epigenetic mechanisms which control chromatin transcriptional states cells. In this review, we discuss evidence that underlie sex-specific neurobehavioral changes during critical organizational periods, estrous cycle, response diverse environments life. We further identify future directions for field will provide novel mechanistic insights into differences, inform disease treatments women's health particular, apply people genders.

Language: Английский

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Deconstructing sex: Strategies for undoing binary thinking in neuroendocrinology and behavior

Hormones and Behavior, Journal Year: 2023, Volume and Issue: 156, P. 105441 - 105441

Published: Oct. 19, 2023

The scientific community widely recognizes that "sex" is a complex category composed of multiple physiologies. Yet in practice, basic research often treats as single, internally consistent, and binary variable. This practice occludes important physiological factors processes, thus limits the value our findings. In human-oriented biomedical research, use simplistic (and binary) models sex ignores existence intersex, trans, non-binary, gender non-conforming people contributes to medical paradigm neglects their needs interests. More broadly, collective reliance on these legitimizes false human biology undergirds harmful practices anti-trans political movements. Herein, we continue conversations begun at SBN 2022 Symposium Hormones Trans Health, providing guiding questions help scientists deconstruct rethink across stages method. We offer step toward more accurately represents sexed physiologies multiple, interacting, variable, unbounded by gendered preconceptions. hope this paper will serve useful resource for who seek new researching understanding improves science, widens applicability findings, deters misuse against marginalized groups.

Language: Английский

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Recommendations for a Better Understanding of Sex and Gender in the Neuroscience of Mental Health

Biological Psychiatry Global Open Science, Journal Year: 2023, Volume and Issue: 4(2), P. 100283 - 100283

Published: Dec. 30, 2023

There are prominent sex/gender differences in the prevalence, expression and lifespan course of mental health neurodiverse conditions. Yet underlying sex gender related mechanisms their interactions still not fully understood. This lack knowledge has harmful consequences for those suffering from problems. Hence, we set up a co-creation session one week workshop with multidisciplinary team 25 researchers, clinicians policy makers, to identify main barriers research neuroscience health. Based on this work, here provide recommendations methodologies, translational stakeholder involvement. These include guidelines recording, reporting, analysis beyond binary groups, open science. Improved understanding may benefit public as is an important step towards precision medicine function archetype studying diversity.

Language: Английский

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A developmental framework for understanding the influence of sex and gender on health: Pediatric pain as an exemplar

Neuroscience & Biobehavioral Reviews, Journal Year: 2024, Volume and Issue: 158, P. 105546 - 105546

Published: Jan. 24, 2024

Sex differences are a robust finding in many areas of adult health, including cardiovascular disease, psychiatric disorders, and chronic pain. However, sex not consistently observed until after the onset puberty. This has led to hypothesis that hormones primary contributors health outcomes, largely ignoring relative contributions early developmental influences, emerging psychosocial factors, gender, interaction between these variables. In this paper, we argue comprehensive understanding gender outcomes should start as conception take an iterative biopsychosocial-developmental perspective considers intersecting social positions. We present conceptual framework, informed by review literature basic, clinical, science captures how critical stages for both can affect children's longer-term outcomes. The on pediatric pain is used worked example framework be applied different conditions.

Language: Английский

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Practical solutions for including sex as a biological variable (SABV) in preclinical neuropsychopharmacological research

Journal of Neuroscience Methods, Journal Year: 2023, Volume and Issue: 401, P. 110003 - 110003

Published: Nov. 1, 2023

Language: Английский

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Focus on fentanyl in females: Sex and gender differences in the physiological and behavioral effects of fentanyl

Frontiers in Neuroendocrinology, Journal Year: 2023, Volume and Issue: 71, P. 101096 - 101096

Published: Aug. 18, 2023

Language: Английский

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Sex differences in socioemotional behavior and changes in ventral hippocampal transcription across aging in C57Bl/6J mice

Neurobiology of Aging, Journal Year: 2023, Volume and Issue: 130, P. 141 - 153

Published: June 26, 2023

Language: Английский

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