A glutamine metabolish-associated prognostic model to predict prognosis and therapeutic responses of hepatocellular carcinoma DOI Creative Commons

Hao Xu,

Hui‐Lin Pan, Fang Lian

et al.

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 20, 2024

Hepatocellular carcinoma (HCC) ranks among the most lethal malignancies around world. However, current management strategies for predicting prognosis in HCC patients remain unreliable. Our study developed a robust prognostic model based on glutamine metabolism associated-genes (GMAGs), utilizing data from The Cancer Genome Atlas database. values of were validated through databases Gene Expression Omnibus and International Consortium via Kaplan‒Meier curves receiver operating characteristic (ROC). potential biological pathways associated with risk investigated different enrichment analysis, variation analysis. correlation between therapeutic responses analyzed. Quantitative real-time PCR (qRT-PCR) cellular experiments measured to analyze GMAGs. Consequently, was constructed 4 GMAGs (RRM1, RRM2, G6PD, GPX7) least absolute shrinkage selection operator (LASSO) regression ROC showed reliable predictive capacity (p < 0.05). analyses revealed multitude that are significantly cancer. Patients high might be sensitive immunotherapy results qRT-PCR all exhibited higher expression levels samples compared normal Moreover, knockdown RRM1 suppresses progression cells. In this study, we GMAGs, identified as target HCC.

Language: Английский

Single-cell deconvolution algorithms analysis unveils autocrine IL11-mediated resistance to docetaxel in prostate cancer via activation of the JAK1/STAT4 pathway DOI Creative Commons

Bisheng Cheng,

Lingfeng Li, Tianlong Luo

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2024, Volume and Issue: 43(1)

Published: March 1, 2024

Abstract Background Docetaxel resistance represents a significant obstacle in the treatment of prostate cancer. The intricate interplay between cytokine signalling pathways and transcriptional control mechanisms cancer cells contributes to chemotherapeutic resistance, yet underlying molecular determinants remain only partially understood. This study elucidated novel mechanism mediated by autocrine interaction interleukin-11 (IL-11) its receptor subunit alpha(IL-11RA), culminating activation JAK1/STAT4 axis subsequent upregulation oncogene c-MYC. Methods Single-cell secretion profiling organoid was analyzed determine production profiles associated with docetaxel resistance.Analysis expression pattern downstream IL-11RA enrichment signal pathway clarify potential IL-11.Next, chromatin immunoprecipitation coupled high-throughput sequencing (ChIP-seq) performed detect nuclear localization DNA-binding patterns phosphorylated STAT4 (pSTAT4). Coimmunoprecipitation reporter assays were utilized assess pSTAT4 cotranscription factor CREB-binding protein (CBP) as well their role c-MYC activity. Results Autocrine IL-11 markedly increased docetaxel-resistant cells. stimulation resulted robust signalling. Upon activation, translocated nucleus CBP at promoter region, amplifying Inhibition IL-11/IL-11RA or disruption significantly reduced entry binding CBP, leading downregulation restoration sensitivity. Conclusion Our findings identify an loop that confers through pathway. pSTAT4-CBP serves critical enhancer activity Targeting this presents therapeutic strategy overcome advanced Graphical

Language: Английский

Citations

7
Osalmid sensitizes clear cell Renal Cell Carcinoma to navitoclax through a STAT3/BCL-XL pathway DOI Creative Commons
Yizheng Xue, Tianyi Chen, Z. Ma

et al.

Cancer Letters, Journal Year: 2025, Volume and Issue: 613, P. 217514 - 217514

Published: Jan. 31, 2025

Language: Английский

Citations

0
Novel composite nano-frameworks for prostate cancer therapy via synergistic targeting of ribonucleotide reductase DOI Creative Commons
Mingwei Xu, Jun Chen,

Chuan-Xi Huang

et al.

Arabian Journal of Chemistry, Journal Year: 2025, Volume and Issue: 0, P. 1 - 9

Published: April 7, 2025

Language: Английский

Citations

0
The efferocytosis process in aging: Supporting evidence, mechanisms, and therapeutic prospects for age-related diseases DOI Creative Commons
Meng Zhang, Wei Jin,

Yu Sun

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: March 1, 2024

Aging is characterized by an ongoing struggle between the buildup of damage caused a combination external and internal factors. has different effects on phagocytes, including impaired efferocytosis. A deficiency in efferocytosis can cause chronic inflammation, aging, several other clinical disorders. Our review underscores possible feasibility extensive scope employing dual targets various age-related diseases to reduce occurrence progression diseases, ultimately fostering healthy aging increasing lifespan. Key scientific concepts Hence, concurrent implementation strategies aimed at augmenting efferocytic mechanisms anti-aging treatments potential serve as potent intervention for extending duration In this review, we comprehensively discuss concept physiological Subsequently, investigated association hallmarks aging. Finally, growing evidence regarding therapeutic interventions disorders, focusing processes

Language: Английский

Citations

3
FOXA1-dependent PUS1 regulates EIF3b stability in a non-enzymatic pathway mediating prostate cancer bone metastasis DOI Creative Commons

Yongxin Wu,

Shengmeng Peng,

Bisheng Cheng

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(11), P. 4566 - 4584

Published: Jan. 1, 2024

Bone metastasis is a significant contributor to the poor prognosis in prostate cancer. Recent evidence highlights pivotal role of pseudouridine synthases solid tumor progression, yet specific enzyme driving cancer remains unidentified. This study uncovers novel regulatory mechanism FOXA1/PUS1/EIF3b signaling axis bone metastasis. We identified elevated PUS1 expression tissues, correlating with higher clinical grade and worse prognosis. Knockdown inhibited independently its enzymatic activity, EIF3b acting as downstream effector, protected from ubiquitin-mediated degradation by PUS1. Overexpression countered suppression due knockdown. Additionally, FOXA1 was shown enhance binding promoter. Mogroside IV-E, inhibitor, demonstrated potent anti-metastatic effects reducing expression. Our findings highlight critical mediator suggest that targeting this pathway could be promising therapeutic strategy.

Language: Английский

Citations

1
Correction: The key cellular senescence related molecule RRM2 regulates prostate cancer progression and resistance to docetaxel treatment DOI Creative Commons

Bisheng Cheng,

Lingfeng Li,

Yongxin Wu

et al.

Cell & Bioscience, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 1, 2024

Language: Английский

Citations

0
Significance of Ribonucleoside-diphosphate Reductase Subunit M2 in Lung Adenocarcinoma DOI
Xiaojun Wang,

Yun-Xia Huo,

Pengjun Yang

et al.

Current Gene Therapy, Journal Year: 2024, Volume and Issue: 25(2), P. 136 - 156

Published: June 13, 2024

Introduction: The Ribonucleoside-diphosphate Reductase subunit M2 (RRM2) is known to be overexpressed in various cancers, though its specific functional implications remain unclear. This aims elucidate the role of RRM2 progression Lung Adenocarcinoma (LUAD) by exploring involvement and potential impact. Methods: data were sourced from multiple databases assess diagnostic prognostic significance LUAD. We evaluated association between expression immune cell infiltration, analyzed function, explored effects modulating on LUAD characteristics through laboratory experiments. Results: was significantly upregulated tissues cells compared normal counterparts (p < 0.05), with rare genetic alterations noted (approximately 2%). overexpression clearly distinguished tissue (area under curve (AUC): 0.963, 95% confidence intervals (CI): 0.946-0.981). Elevated associated adverse clinicopathological poor prognosis patients. Furthermore, a positive observed infiltration. Pathway analysis revealed critical connection cycle signaling pathway within Targeting inhibition effectively suppressed proliferation, migration, invasion while promoting apoptosis. intervention also modified several crucial proteins, including downregulation CDC25A, CDC25C, RAD1, Bcl-2, PPM1D upregulation TP53 Bax 0.05). Conclusion: Our findings highlight utility as biomarker for diagnosing predicting LUAD, shedding new light this malignancy.

Language: Английский

Citations

0
Predicting Survival in Patients with Neuroendocrine Prostate Cancer: A SEER-Based Comprehensive Study DOI Creative Commons
Tianlong Luo, Jintao Hu, Bisheng Cheng

et al.

The World Journal of Men s Health, Journal Year: 2024, Volume and Issue: 42

Published: Jan. 1, 2024

Neuroendocrine prostate cancer (NEPC) represents a particularly aggressive subtype of with challenging prognosis. The purpose this investigation is to craft and confirm the reliability nomograms that can accurately forecast 1-, 3-, 5-year overall survival (OS) cancer-specific (CSS) rates for individuals afflicted NEPC.

Language: Английский

Citations

0
A glutamine metabolish-associated prognostic model to predict prognosis and therapeutic responses of hepatocellular carcinoma DOI Creative Commons

Hao Xu,

Hui‐Lin Pan, Fang Lian

et al.

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 20, 2024

Hepatocellular carcinoma (HCC) ranks among the most lethal malignancies around world. However, current management strategies for predicting prognosis in HCC patients remain unreliable. Our study developed a robust prognostic model based on glutamine metabolism associated-genes (GMAGs), utilizing data from The Cancer Genome Atlas database. values of were validated through databases Gene Expression Omnibus and International Consortium via Kaplan‒Meier curves receiver operating characteristic (ROC). potential biological pathways associated with risk investigated different enrichment analysis, variation analysis. correlation between therapeutic responses analyzed. Quantitative real-time PCR (qRT-PCR) cellular experiments measured to analyze GMAGs. Consequently, was constructed 4 GMAGs (RRM1, RRM2, G6PD, GPX7) least absolute shrinkage selection operator (LASSO) regression ROC showed reliable predictive capacity (p < 0.05). analyses revealed multitude that are significantly cancer. Patients high might be sensitive immunotherapy results qRT-PCR all exhibited higher expression levels samples compared normal Moreover, knockdown RRM1 suppresses progression cells. In this study, we GMAGs, identified as target HCC.

Language: Английский

Citations

0