Consumer attitudes toward food crops developed by CRISPR/Cas9 in Costa Rica

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2019, Volume and Issue: 139(2), P. 417 - 427

Published: July 4, 2019

Language: Английский

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Strategies to combat the problem of yam anthracnose disease: Status and prospects

Molecular Plant Pathology, Journal Year: 2021, Volume and Issue: 22(10), P. 1302 - 1314

Published: July 17, 2021

Yam (Dioscorea spp.) anthracnose, caused by Colletotrichum alatae, is the most devastating fungal disease of yam in West Africa, leading to 50%-90% tuber yield losses severe cases. In some instances, plants die without producing any tubers or each shoot may produce several small before it dies if strikes early. C. alatae affects all parts plant at stages development, including leaves, stems, tubers, and seeds yams, highly prevalent belt region other yam-producing countries world. Traditional methods adopted farmers control have not been very successful. Fungicides also failed provide long-lasting control. Although conventional breeding genomics-assisted used develop level resistance anthracnose Dioscorea alata, appearance new more virulent strains makes development improved varieties with broad-spectrum durable critical. These shortcomings, coupled interspecific incompatibility, dioecy, polyploidy, poor flowering, long cycle crop, prompted researchers explore biotechnological techniques complement speed up crop improvement. Modern tools potential fungus-resistant cultivars, thereby bypassing natural bottlenecks traditional breeding. This article reviews existing strategies proposes approaches that could be anthracnose-resistant for food security Africa.

Language: Английский

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Unintended Genomic Outcomes in Current and Next Generation GM Techniques: A Systematic Review

Plants, Journal Year: 2022, Volume and Issue: 11(21), P. 2997 - 2997

Published: Nov. 7, 2022

Classical genetic engineering and new genome editing techniques, especially the CRISPR/Cas technology, increase possibilities for modifying material in organisms. These technologies have potential to provide novel agricultural traits, including modified microorganisms environmental applications. However, legitimate safety concerns arise from unintended modifications (GM) that been reported as side-effects of such techniques. Here, we systematically review scientific literature studies investigated genomic alterations plants by following GM techniques: Agrobacterium tumefaciens-mediated gene transfer, biolistic bombardment, CRISPR-Cas9 delivered via Agrobacterium-mediated transfer (DNA-based), bombardment (DNA-based) ribonucleoprotein complexes (RNPs). The results our show impact techniques host genomes varies small nucleotide polymorphisms large variation, segmental duplication, chromosome truncation, trisomy, chromothripsis, breakage fusion bridge, rearrangements DNA vector-backbone sequences. We also reviewed type analytical method applied investigate found only five articles used whole sequencing their analysis methods. In addition, larger structural variations detected some would not be possible without long-read strategies, which shows a underestimation effects literature. As are constantly evolving, more thorough examination prospective methods should conducted future. This will regulators working field genetically gene-edited organisms with valuable information on ability detect identify interventions.

Language: Английский

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Genome editing for improving nutritional quality, post-harvest shelf life and stress tolerance of fruits, vegetables, and ornamentals

Frontiers in Genome Editing, Journal Year: 2023, Volume and Issue: 5

Published: Feb. 24, 2023

Agricultural production relies on horticultural crops, including vegetables, fruits, and ornamental plants, which sustain human life. With an alarming increase in population the consequential need for more food, it has become necessary increased to maintain food security. Conventional breeding subsidized development of improved verities but enhance crop production, new techniques be acquired. CRISPR-Cas9 system is a unique powerful genome manipulation tool that can change DNA precise way. Based bacterial adaptive immune system, this technique uses endonuclease creates double-stranded breaks (DSBs) at target loci under guidance single guide RNA. These DSBs repaired by cellular repair mechanism installs small insertion deletion (indels) cut sites. When equated alternate editing tools like ZFN, TALENs, meganucleases, CRISPR- The cas-based quickly gained fast-forward its simplicity, ease use, low off-target effect. In numerous industrial CRISPR technology been successfully used stress tolerance, self-life, nutritional improvements, flavor, metabolites. CRISPR-based most appropriate one with prospective goal generating non-transgenic yields avoiding regulatory hurdles release modified crops into market. Although several challenges horticultural, industrial, remain, novel nuclease, crop-specific application, makes dynamic improvement.

Language: Английский

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Application of CRISPR/Cas-based gene-editing for developing better banana

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 16, 2024

Banana ( Musa spp.), including plantain, is one of the major staple food and cash crops grown in over 140 countries subtropics tropics, with around 153 million tons annual global production, feeding about 400 people. Despite its widespread cultivation adaptability to diverse environments, banana production faces significant challenges from pathogens pests that often coexist within agricultural landscapes. Recent advancements CRISPR/Cas-based gene editing offer transformative solutions enhance resilience productivity. Researchers at IITA, Kenya, have successfully employed confer resistance diseases such as Xanthomonas wilt (BXW) by targeting susceptibility genes streak virus (BSV) disrupting viral sequences. Other breakthroughs include development semi-dwarf plants, increased β-carotene content. Additionally, non-browning been developed reduce waste, regulatory approval Philippines. The future prospects looks promising CRISPR-based activation (CRISPRa) inhibition (CRISPRi) techniques offering potential for improved disease resistance. Cas-CLOVER system provides a precise alternative CRISPR/Cas9, demonstrating success generating gene-edited mutants. Integration precision genetics traditional breeding, adopting transgene-free strategies, will be pivotal harnessing full banana. crop holds exciting producing thrives across agroecological zones offers superior nutritional value, ultimately benefiting farmers consumers. This article highlights role CRISPR/Cas technology advancing resilience, yield quality, implications security.

Language: Английский

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