The NLRP3 molecule influences the therapeutic effects of mesenchymal stem cells through Glut1-mediated energy metabolic reprogramming DOI Creative Commons

Jingrou Chen,

Shu‐Juan Xie, Dongbo Qiu

et al.

Journal of Advanced Research, Journal Year: 2023, Volume and Issue: 65, P. 125 - 136

Published: Dec. 7, 2023

Numerous studies demonstrated that NLRP3 has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Mesenchymal stem cells (MSCs) regulated inflammasome, which emerged as a novel therapeutic approach for treating IBD. The exact role regulating MSCs' function is unclear. Our study aimed to explore how affects effects MSCs colitis. We extracted from bone marrow C57/B6 mice and Nlrp3 KO mice, identified them using differentiation assays flow cytometry. In vitro, Both WT were stimulated with factor Lipopolysaccharide (LPS), only varying concentrations inhibitor MCC950, then, quantified IL-10 levels supernatant. RNA-seq was performed examine gene expression patterns Seahorse used assess oxidative phosphorylation (OXPHOS) glycolysis levels. Western blot evaluate protein expression. vivo, we treated DSS-induced colitis either or MSCs, monitoring weight, measuring colon length, further evaluation. also pretreated (BAY876, oe-Glut1, oe-NLRP3), following same experimental procedures described above. results demonstrate deletion did not affect MSC phenotypes, but rather promoted osteogenic differentiation. However, absence reduced production presence LPS, leading impaired protection on Conversely, overexpression promotes IL-10, enhancing effects. Further investigation revealed deficiency downregulated Glut1 activation resulting decreased production. Notably, overexpressing restored their effect previously dampened due deletion. findings heightens treatment

Language: Английский

Exploring the ethical considerations of using Chat GPT in university education DOI Open Access

et. al. Jorge Jinchuña Huallpa

Periodicals of Engineering and Natural Sciences (PEN), Journal Year: 2023, Volume and Issue: 11(4), P. 105 - 105

Published: Aug. 30, 2023

This study investigates the moral dilemmas that arise with incorporating Chat GPT into higher education, a focus on situation in Latinoamerican institutions of learning. The surveyed 220 people via online questionnaire to learn more about their experiences and motivations for using AI-powered conversational agents. An overview demographics participants was provided through descriptive statistics. investigation subject at hand lays groundwork further research. It also reveals hidden meanings observed phenomena, it suggests possible solutions problems have been uncovered. research looks how AI systems chatbots can supplement human knowledge judgment, as well potential drawbacks. results showed thought integration moderately accessible had positive social attitudes. They understood value responsibility creating individualized educational opportunities. Participants stressed necessity explicit institutional standards regarding privacy data security. Gender, age, sense accessibility, attitude, opinions, personal experience, security, guidelines, learning were found affect participants' reliance regression analysis. findings shed light education is complicated by factors such individual beliefs, cultural norms, ethical problems. busy schedules students may be accommodated resources they need succeed made available thanks this adaptability. In addition, natural language processing models offer instantaneous help text chat, voice, or video. To fully grasp consequences lead creation responsible implementation techniques, proposes additional qualitative investigations, longitudinal studies, comparative across diverse contexts required. Closing these gaps will move field forward ways are beneficial classroom.

Language: Английский

Citations

65
Assessing the drug delivery of ibuprofen by the assistance of metal-doped graphenes: Insights from density functional theory DOI

Sahar Esfahani,

Jafar Akbari,

Somayeh Soleimani‐Amiri

et al.

Diamond and Related Materials, Journal Year: 2023, Volume and Issue: 135, P. 109893 - 109893

Published: March 28, 2023

Language: Английский

Citations

34
Recent advances in biomaterials for tissue-engineered constructs: Essential factors and engineering techniques DOI Creative Commons

Shiva Norouzi,

Nikoo Saveh Shemshaki,

Ehsan Norouzi

et al.

Materials Today Chemistry, Journal Year: 2024, Volume and Issue: 37, P. 102016 - 102016

Published: April 1, 2024

Tissue-engineered constructs can replicate the structural and physiological properties of natural tissues. The be designed to address transplantation issues affected by shortage donor tissues organs. One major concerns in tissue engineering is design development structures that improve interaction between materials cells provide an ideal platform for form functional tissue. Several contributing factors need considered fabricate constructs, including biomaterials, biological, topographical, biophysical, morphological either alone or combination. Here, we review application, advancement, future directions these essential designing developing regeneration. In particular, focus on original approaches tools construct parameters engineering.

Language: Английский

Citations

13
The impact of interleukin-6 (IL-6) and mesenchymal stem cell-derived IL-6 on neurological conditions DOI Creative Commons
Irina Kerkis, Álvaro Rossan de Brandão Prieto da Silva, Rodrigo Pinheiro Araldi

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 24, 2024

Interleukin-6 (IL-6) is a versatile cytokine crucial for immune response modulation, inflammation regulation, and various physiological processes in the body. Its wide-ranging functions underscore its importance maintaining health. Dysregulated IL-6 closely associated with many diseases, making it key research therapeutic target. Elevated levels central nervous system worsen neuroinflammation neurodegenerative diseases by activating microglia astrocytes releasing pro-inflammatory cytokines neurotoxic molecules. Moreover, dysregulated weakens blood-brain barrier, exacerbating neuronal damage allowing peripheral cells inflammatory mediators to enter brain. Mesenchymal stem (MSCs) show promise modulating regulating levels. They effectively suppress cytokines, including IL-6, while promoting anti-inflammatory factors. This approach highlights of targeting other alleviate adverse effects on neurological disorders. review provides comprehensive overview IL-6’s involvement disorders, examining endogenous derived from MSCs. We explore mechanisms affecting function, survival, modulation system. Additionally, we discuss potential MSC-derived neuroregeneration neuroprotection. By elucidating interplay pathologies, this offers insights into novel strategies signaling pathways

Language: Английский

Citations

13
Biomaterials for inflammatory bowel disease: treatment, diagnosis and organoids DOI
Jia Wang, Yuying Shi,

Bei Mao

et al.

Applied Materials Today, Journal Year: 2024, Volume and Issue: 36, P. 102078 - 102078

Published: Jan. 20, 2024

Language: Английский

Citations

9
Advancements and Challenges in Stem Cell Transplantation for Regenerative Medicine DOI Creative Commons

Lingxi Wei,

Wenqi Yan,

Wahid Shah

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e35836 - e35836

Published: Aug. 1, 2024

Stem cell transplantation has emerged as a promising avenue in regenerative medicine, potentially facilitating tissue repair degenerative diseases and injuries. This review comprehensively examines recent developments challenges stem transplantation. It explores the identification isolation of various types, including embryonic, induced pluripotent, adult cells derived from multiple sources. Additionally, highlights tissue-specific applications these cells, focusing on bone cartilage regeneration, treatment neurological disorders, management hematological conditions. Future advancements effective resolution current will be crucial fully realizing potential medicine. With responsible ethical practices, field can transform disease injury treatment, ultimately improving quality life for countless individuals.

Language: Английский

Citations

9
Recent advances in mesenchymal stem cell therapy for multiple sclerosis: clinical applications and challenges DOI Creative Commons

Kamran Sheikhi,

Sara Ghaderi,

Hassan Firouzi

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 3, 2025

Multiple sclerosis (MS), a chronic autoimmune disorder of the central nervous system (CNS), is characterized by inflammation, demyelination, and neurodegeneration, leading to diverse clinical manifestations such as fatigue, sensory impairment, cognitive dysfunction. Current pharmacological treatments primarily target immune modulation but fail arrest disease progression or entirely reverse CNS damage. Mesenchymal stem cell (MSC) therapy offers promising alternative, leveraging its immunomodulatory, neuroprotective, regenerative capabilities. This review provides an in-depth analysis MSC mechanisms action, including regulation, promotion remyelination, neuroregeneration. It examines preclinical studies trials evaluating efficacy, safety, limitations in various MS phenotypes. Special attention given challenges delivery routes, dosing regimens, integrating MSCs with conventional therapies. By highlighting advancements ongoing challenges, this underscores potential revolutionize treatment, paving way for personalized combinatory therapeutic approaches.

Language: Английский

Citations

1
Tedizolid phosphate alleviates DSS-induced ulcerative colitis by inhibiting senescence of cell and colon tissue through activating AMPK signaling pathway DOI
Min Zhou,

Zhenlin Liu,

Jiayu Liu

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 135, P. 112286 - 112286

Published: May 21, 2024

Language: Английский

Citations

8
Mesenchymal Stromal Cells: New Generation Treatment of Inflammatory Bowel Disease DOI Creative Commons

Shulin Wei,

Mingxing Li, Qin Wang

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 3307 - 3334

Published: May 1, 2024

Abstract: Inflammatory bowel disease (IBD) is a chronic inflammatory of the gastrointestinal tract, which has high recurrence rate and incurable due to lack effective treatment. Mesenchymal stromal cells (MSCs) are class pluripotent stem that have recently received lot attention their strong self-renewal ability immunomodulatory effects, large number experimental clinical models confirmed positive therapeutic effect MSCs on IBD. In preclinical studies, MSC treatment for IBD relies paracrine cell-to-cell contact, its mediated mitochondrial transfer immune regulation. It also plays role in restoring intestinal mucosal barrier through homing effect, regulation microbiome, repair epithelial cells. latest trials, safety efficacy been by transfusion autologous or allogeneic bone marrow, umbilical cord, adipose MSCs, as well derived extracellular vesicles. However, regarding stable use several concerns emerge, including cell sources, management (dose, route frequency administration, pretreatment MSCs) adverse reactions. This article comprehensively summarizes effects mechanisms advantages over conventional drugs, trial progress The current challenges future directions discussed. review would add knowledge into understanding applying MSCs. Keywords: mesenchymal cells, immunomodulation, disease, ulcerative colitis, Crohn's therapy

Language: Английский

Citations

7
Therapeutic potential of mesenchymal stem cell‐derived extracellular vesicles: A focus on inflammatory bowel disease DOI Creative Commons
L Clua-Ferré, R Suau,

Irene Vañó‐Segarra

et al.

Clinical and Translational Medicine, Journal Year: 2024, Volume and Issue: 14(11)

Published: Nov. 1, 2024

Abstract Background Mesenchymal stem cell‐derived extracellular vesicles (MSC‐EVs) have emerged as key regulators of intercellular communication, orchestrating essential biological processes by delivering bioactive cargoes to target cells. Available evidence suggests that MSC‐EVs can mimic the functions their parental cells, exhibiting immunomodulatory, pro‐regenerative, anti‐apoptotic, and antifibrotic properties. Consequently, represent a cell‐free therapeutic option for patients with inflammatory bowel disease (IBD), overcoming limitations associated cell replacement therapy, including non‐immunogenic nature, lower risk tumourigenicity, cargo specificity ease manipulation storage. Main Topics Covered This review aims provide comprehensive examination efficacy in IBD, focus on mechanisms action potential impact treatment outcomes. We examine advantages over traditional therapies, discuss methods isolation characterisation, present mechanistic insights into effects through transcriptomic, proteomic lipidomic analyses MSC‐EV cargoes. also available preclinical studies demonstrating reduce inflammation, promote tissue repair restore intestinal homeostasis IBD models, compare these findings those clinical trials. Conclusions Finally, we highlight novel therapy identify challenges opportunities translation practice. Highlights The source mesenchymal cells (MSCs) strongly influences composition function MSC‐derived (EVs), affecting potential. Adipose‐derived MSC‐EVs, known immunoregulatory properties isolation, show promise (IBD). MicroRNAs are consistently across types involved pathways dysregulated making them agents. For example, miR‐let‐7a is inhibition apoptosis, miR‐100 supports survival, miR‐125b helps suppress pro‐inflammatory cytokines miR‐20 promotes anti‐inflammatory M2 macrophage polarisation. Preclinical models shown inflammation suppressing mediators (e.g., TNF‐α, IL‐1β, IL‐6) increasing factors IL‐4, IL‐10). They mucosal healing strengthen integrity gut barrier, suggesting address pathology.

Language: Английский

Citations

7