Metabolic pathways of glucose and fructose: II Spatiotemporal expression of genes involved in synthesis and transport of lactate in ovine conceptuses

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 111(1), P. 159 - 173

Published: March 26, 2024

Lactate, an abundant molecule in fetal fluids and blood of mammalian species, is often overlooked as a metabolic waste product generated during pregnancy. Most the glucose fructose consumed by ovine conceptuses converted to lactate, but proteins involved lactate metabolism transport have not been investigated. This study characterized total produced throughout gestation, well expression mRNAs metabolism. Lactate increased abundance uterine lumen preimplantation period was more than pyruvate. The allantoic amniotic with advancing days gestation most on Day 125 pregnancy (P < 0.05). dehydrogenase subunits A (converts pyruvate lactate) B pyruvate) were expressed gestation. transported via monocarboxylic acid transporters SLC16A1 SLC16A3, both which conceptus Additionally, interplacentomal chorioallantois from 126 SLC16A3 across tissue. Hydrocarboxylic receptor 1 (HCAR1), for localized luminal superficial glandular epithelia pregnant ewes trophectoderm peri-implantation These results provide novel insights into spatiotemporal profiles enzymes, transporters,

Language: Английский

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Early Embryonic Development in Agriculturally Important Species

Animals, Journal Year: 2024, Volume and Issue: 14(13), P. 1882 - 1882

Published: June 26, 2024

The fertilization of oocytes ovulated by pigs, sheep, cows, and horses is not considered a limiting factor in successful establishment pregnancy. Pig, cow embryos undergo cleavage to the blastocyst stage, hatch from zona pellucida, central-type implantation. Hatched blastocysts cows transition tubular long filamentous forms establish surface area for exchange nutrients gases with uterus. equine blastocyst, surrounded external membranes, does elongate but migrates throughout uterine lumen before attaching luminal epithelium (LE) begin Pregnancy recognition signaling pigs requires trophectoderm express interleukin 1 beta, estrogens, prostaglandin E2, interferon gamma. Sheep conceptus expresses tau that induces regulatory 2 inhibits transcription estrogen oxytocin receptors epithelia. This prevents oxytocin-induced luteolytic pulses F2-alpha regressing corpora lutea, as well ensuring secretion progesterone required maintenance pregnancy signal produced known. Implantation these species interactions between extracellular matrix (ECM) proteins integrins undergoes apposition firm attachment LE. review provides details respect early embryonic development spherical conceptuses pre-implantation implantation conceptuses.

Language: Английский

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Metabolic pathways for glucose and fructose: I synthesis and metabolism of fructose by ovine conceptuses

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 111(1), P. 148 - 158

Published: March 19, 2024

Fructose, the most abundant hexose sugar in fetal fluids and blood of sheep other ungulates cetaceans, is synthesized from glucose via polyol pathway trophectoderm chorion. However, cell-specific temporal expression enzymes for synthesis metabolism fructose conceptuses (embryo placental membranes) placentomes has not been characterized. This study characterized key involved by ovine throughout pregnancy. Day 17 expressed mRNAs (SORD AKR1B1) (HK1, HK2, G6PD, OGT, FBP), but those required gluconeogenesis (G6Pase or PCK). Ovine also SORD, AKR1B1, HK1, OGT. Fructose can be metabolized ketohexokinase (KHK) pathway, isoforms, KHK-A KHK-C, were 16 pregnancy during a manner. The protein was more cotyledons placentomes, while KHK-C endoderm chorionic epithelium placentomes. Expression KHK greatest at 30 (P < 0.05), different among days later gestation. These results provide novel insights into uninhibited to generate ATP tricarboxylic cycle, as well substrates pentose hexosamine biosynthesis one-carbon conceptus development

Language: Английский

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CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy

Cells, Journal Year: 2023, Volume and Issue: 12(21), P. 2554 - 2554

Published: Oct. 31, 2023

The successful establishment of endometrial receptivity is a key factor in ensuring the fertility ewes and their economic benefits. Hu sheep have attracted attention due to high fecundity year-round estrus. In this study, we found that luteal phase, uterine gland density, coefficient, number caruncles high-fertility were higher than those low-fertility sheep. Thousands differentially expressed genes identified endometrium with different potential using RNA sequencing (RNA-Seq). Several involved screened bioinformatics analysis. qRT-PCR analysis further revealed differential expression cAMP reactive element binding protein-1 (CREB1) during estrous cycle. Functionally, our results suggested CREB1 significantly affected level marker genes, promoted cell proliferation by facilitating transition from G1 phase S inhibited apoptosis autophagy. Moreover, observed negative linear correlation between miR-134-5p endometrium. addition, overexpression prevented effect on stromal (ESC) growth. Taken together, these data indicated was regulated may promote regulating function ESCs. study provides new theoretical references for identifying candidate associated fertility.

Language: Английский

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Hormones and reproductive cycles in ungulates

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 365 - 375

Published: Jan. 1, 2024

Language: Английский

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Regulation of synthesis of polyamines by progesterone, estradiol, and their receptors in uteri of cyclic ewes

Biology of Reproduction, Journal Year: 2023, Volume and Issue: 109(3), P. 309 - 318

Published: July 4, 2023

Progesterone (P4), estradiol (E2), and expression of their receptors (PGR ESR1, respectively) by cells the uterus regulate reproductive performance mammals through effects on secretion transport nutrients into uterine lumen. This study investigated effect changes in P4, E2, PGR, ESR1 enzymes for synthesis polyamines. Suffolk ewes (n = 13) were synchronized to estrus (Day 0) then, either Day 1 (early metestrus), 9 diestrus), or 14 (late diestrus) estrous cycle, maternal blood samples collected, euthanized before obtaining flushings. Endometrial MAT2B SMS mRNAs increased late diestrus (P < 0.05). Expression ODC1 SMOX decreased from early metestrus diestrus, ASL mRNA was lower than Immunoreactive PAOX, SAT1, proteins localized luminal, superficial glandular, glandular epithelia, stromal cells, myometrium, vessels. Concentrations spermidine spermine plasma further The abundances flushings less These results indicate that polyamines are affected P4 as well PGR endometria cyclic ewes.

Language: Английский

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Characterization of TNSALP expression, localization, and activity in ovine utero-placental tissues

Biology of Reproduction, Journal Year: 2023, Volume and Issue: 109(6), P. 954 - 964

Published: Sept. 7, 2023

Abstract Tissue-nonspecific alkaline phosphatase (TNSALP; encoded by ALPL gene) has a critical role in the regulation of phosphate homeostasis postnatally. However, utero-placental expression TNSALP and transport pregnancy is poorly understood. Estrous cycles ewes were synchronized, euthanized hysterectomized on Days 1, 9, or 14 estrous cycle bred to fertile rams 12, 17, 30, 50, 70, 90, 110, 125 pregnancy. The mRNA, immunolocalization protein, quantification localization enzymatic activity was performed ovine endometria placentomes. Day did not alter mRNA TNSALP. protein localized uterine epithelial stromal cells, blood vessels, myometrium, caruncular, cotyledonary stroma. epithelia, caruncular stroma (from 70 gestation), apical surface chorionic epithelia 50 gestation). during early Endometrial downregulated 17 30 gestation (P &lt; 0.05). Expression decreased late placentomes peaked 90 gestation. Collectively, these results suggest potential at maternal–conceptus interface ruminants.

Language: Английский

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Progesterone regulates tissue non-specific alkaline phosphatase (TNSALP) expression and activity in ovine utero-placental tissues

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 3, 2024

Tissue non-specific alkaline phosphatase (TNSALP; encoded by the ALPL gene) has a critical role in postnatal regulation of phosphate homeostasis, yet how TNSALP activity and expression are regulated during pregnancy remain largely unknown. This study tested hypothesis that progesterone (P4) and/or interferon tau (IFNT) regulate sheep. In Exp. 1, ewes were bred received daily intramuscular injections either corn oil vehicle (CO) or 25 mg CO for first 8 days hysterectomized on Day 9, 12, 125 gestation. 2, fitted with intrauterine catheters 7 estrous cycle 50 P4 75 receptor antagonist (RU486) from Days to 15, twice control proteins (CX) IFNT (25 µg/uterine horn/d) 11 15 (treatment groups: + CX; IFNT; RU486 IFNT) 16. endometria administered had greater mRNA than 12. appeared epithelia, stratum compactum stroma, endothelium blood vessels endometrium myometrium On 125, localized uterine epithelial endothelial cells, independent treatment. placentomes treated was detected cells caruncular tissue but not ewes. endometrial homogenates CX lower those Immunoreactive protein mid- deep-glandular epithelia as compared other treatment groups. Enzymatic apical surface deep glandular These results suggest P4, IFNT, regulates utero-placental tissues potential contribute availability is conceptus development pregnancy.

Language: Английский

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Endocrine fibroblast growth factors in domestic animals

Domestic Animal Endocrinology, Journal Year: 2024, Volume and Issue: 89, P. 106872 - 106872

Published: July 9, 2024

Fibroblast growth factors (FGFs) are a group of structurally homologous yet functionally pleiotropic proteins. Canonical and intracellular FGFs have primarily autocrine or paracrine effects. However, the FGF19 subfamily, composed FGF15/19, FGF21, FGF23, act as endocrine hormones that regulate bile acid, metabolic, phosphorus homeostasis, respectively. Current research in human rodent models demonstrates potential these to target various diseases, including disorders inherited hypophosphatemia, chronic liver disease, obesity, insulin resistance. Many diseases targeted for therapeutic use humans pathophysiological overlaps domestic animals. Despite clinical economic impact, little is known about their signaling pathways major animal species compared with laboratory This review aims describe physiology FGFs, discuss current use, summarize contemporary literature regarding animals, focusing on future directions.

Language: Английский

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