Published: Jan. 1, 2025
Language: Английский
Malaria Journal, Journal Year: 2023, Volume and Issue: 22(1)
Published: Jan. 17, 2023
Abstract Since its first report in Anopheles mosquitoes 1950s, insecticide resistance has spread very fast to most sub-Saharan African malaria-endemic countries, where it is predicted seriously jeopardize the success of vector control efforts, leading rebound disease cases. Supported mainly by four mechanisms (metabolic resistance, target site cuticular and behavioural resistance), this phenomenon associated with intrinsic changes resistant insect vectors that could influence development invading Plasmodium parasites. A literature review was undertaken using Pubmed database collect articles evaluating directly or indiretly impact on key determinants malaria competence including sialome composition, anti- immunity, intestinal commensal microbiota, mosquito longevity. Globally, evidence gathered contradictory even though seem be more permissive infections. The actual body knowledge factors vectorial competence, such as immunity microbiota communities still insufficient definitively infer epidemiological importance these against susceptible counterparts. More studies are needed fill important gaps help predicting epidemiology a context selection ongoing.
Language: Английский
Citations
50
Trends in Genetics, Journal Year: 2022, Volume and Issue: 38(7), P. 708 - 723
Published: March 21, 2022
Safe and sustainable approaches for mosquito control are critical due to the global increasing burden of mosquito-transmitted diseases.Novel based on symbionts currently proposed modify or suppress populations Wolbachia-based methods have already achieved some success in field trials.Transgenic mosquitoes carrying gene drives that spread through a promising approach block disease transmission vector species.Transgenic-based potentially offer more power flexibility, but symbiont-based usually socially accepted well-developed. Mosquitoes bring health problems by transmitting parasites viruses such as malaria dengue. Unfortunately, current insecticide-based strategies only moderately effective because high cost resistance. Thus, scalable, sustainable, cost-effective needed mosquito-borne control. Symbiont-based genome engineering-based provide new tools show promise meeting these criteria, enabling modification suppression approaches. Symbiotic bacteria like Wolbachia maternally inherited manipulate host reproduction enhance their vertical transmission. Genome drive methods, which genetically altered alleles throughout wild populations, also proving be powerful laboratory. Here, we review latest developments both drive-based methods. We describe notable similarities, well distinctions obstacles, relating technologies. can found almost anywhere world, tropics subtropics, half world's population is under threat pathogens dengue virus (DENV), Zika (ZIKV), chikungunya (CHIKV), yellow fever, West Nile (WNV), malaria, filarial nematodes [1.World Health Organization Working Overcome Global Impact Neglected Tropical Diseases: First WHO Report Diseases. WHO, 2010Google Scholar,2.Tahir D. et al.Vector-borne nematode diseases pets humans Mediterranean Basin: an update.Vet. World. 2019; 12: 1630-1643Crossref PubMed Scopus (15) Google Scholar]. For example, DENV incidence has grown over 30-fold past 50 years, now reaching about 400 million cases per year [3.Bhatt S. al.The distribution dengue.Nature. 2013; 496: 504-507Crossref (5268) The recent ZIKV outbreak resulted hundreds thousands infections large-scale social economic disruption [4.World Virus Outbreak Response: Interim Report. 2016Google While falling southeast Asia, rising other parts world remain 'unacceptably high' according World [5.Guglielmi G. Malaria worldwide.Nature. (Published online December 4, 2019. https://doi.org/10.1038/d41586-019-03746-3)Crossref Re-emergence expansion many factors, including increased urbanization travel trade, climate change, land use pattern changes, unreliable piped water supply [6.World Vector Control Response 2017-2030. 2017Google Current strategies, long-lasting insecticide-treated bed nets, chemical insecticides, environmental management [7.Schreck C.E. Permethrin dimethyl phthalate tent fabric treatments against Aedes aegypti.J. Am. Mosq. Assoc. 1991; 7: 533-535PubMed Scholar], been unable address genetic behavioral resistance interventions [8.Succo T. al.Autochthonous Nîmes, South France, July September 2015.Euro Surveill. 2016; 21: 21Crossref (0) In addition, unintended effect important nontarget insects, pollinators [9.Ware G.W. Effects pesticides organisms.Residue Rev. 1980; 76: 173-201Crossref (49) new, urgently diseases. response this growing need, number novel technologies expanded years. Many involve release aim achieve (see Glossary) type mosquitoes. Population reduce eliminate populations. Such include sterile insect technique (SIT), incompatible (IIT), transgenic-based technologies, where insects mate with sizes next generation. Gene approaches, increase frequency multiple generations, could after single refractory resistant prevent pathogen symbiont into enable result entire becoming pathogen. review, summarize symbiont-infected transgenic focusing different varieties capabilities. early symbiont-mediated [10.Laven H. Eradication Culex pipiens fatigans cytoplasmic incompatibility.Nature. 1967; 216: 383-384Crossref (275) Scholar] was introduction nonmodified microorganisms competence (Box 1). most extensively studied system natural It may common intracellular endosymbiont arthropods nematodes, 60% all harboring [11.Hilgenboecker K. al.How species infected Wolbachia?--A statistical analysis data.FEMS Microbiol. Lett. 2008; 281: 215-220Crossref transmitted vertically from mother offspring maximize manipulating feminization, parthenogenesis, male killing, and/or incompatibility (CI). CI induced when Wolbachia-infected males uninfected females, results nonviable offspring. inhibit infection DENV, ZIKV, arboviruses, parasite (Figure 1, Key figure ) [12.Bian al.Wolbachia invades Anopheles stephensi induces refractoriness Plasmodium infection.Science. 340: 748-751Crossref (289) Scholar, 13.Dutra H.L. blocks circulating isolates Brazilian aegypti mosquitoes.Cell Host Microbe. 19: 771-774Abstract Full Text PDF 14.Hughes G.L. virulent human falciparum gambiae.PLoS Pathog. 2011; 7e1002043Crossref (214) Transfected native used Scholar,15.Zheng X. al.Incompatible techniques combined mosquitoes.Nature. 572: 56-61Crossref (212) Interestingly, species, aegypti, naturally free [16.Gloria-Soria A. al.Lack evidence (Diptera: Culicidae).J. Med. Entomol. 2018; 55: 1354-1356PubMed Scholar,17.Ross P.A. al.An elusive endosymbiont: does occur aegypti?.Ecol. Evol. 2020; 10: 1581-1591Crossref (27) providing open niche infection. there conjecture if major vectors truly [18.Chrostek E. Gerth M. Is gambiae Wolbachia?.mBio. 10e00784-19Crossref (24) reports indicate Anopheline possess high-density [19.Walker al.Stable moucheti demeilloni mosquitoes.Curr. Biol. 2021; 31: 2310-2320Abstract (12) This offers renewed medically relevant strains adapted environment. Several reviews covered progress exploit bacterium [20.Flores H.A. O'Neill S.L. Controlling vector-borne releasing modified mosquitoes.Nat. 16: 508-518Crossref (145) 21.Wang G.-H. al.Combating using technologies.Nat. Commun. 4388Crossref 22.Caragata E.P. translational science: controlling pathogens.Trends Parasitol. 37: 1050-1067Abstract Scholar].Box 1Using strategiesThe increasingly emerging interactions among hosts, infection, inspiring development [111.Gupta Nair Dynamics insect-microbiome interaction influence microbial symbiont.Front. 11: 1357Crossref (25) Most importantly, shows potential minimize problem cause minimal side effects application includes: (i) delivering directly disrupt physiology display antipathogen effects; (ii) express effector molecules, then engineered so decreased [112.Gao al.Mosquito microbiota implications control.Trends 36: 98-111Abstract (28) vectorial capacity [113.Cansado-Utrilla C. microbiome capacity: rich discovery translation.Microbiome. 9: 111Crossref (9) I).Figure 1Key figure. control.Show full caption(A) Natural bacteria-based (CI) Serratia Y1 Asaia induce immunity slow development. secondary metabolites ureilytica Su_YN1 Chromobacterium responsible antiplasmodial (DENV)-2. (B) fungus-based Wickerhamomyces anomalus secreting toxin protein Beauveria bassiana DENV-2 (ZIKV) activity inducing system. (C) insect-specific (Eilat virus, cell fusing agent Phasi Charoen-like Negevirus) arbovirus development, either alone combination. (D) Engineered AS1 proteins Dirofilaria immitis. (E) Metarhizium pingshaense-infected shorter life spans reproductive output than (F) virus-based Negevirus expressed anti-chikungunya (CHIKV) antibody CHIKV replication.View Large Image Figure ViewerDownload Hi-res image Download (PPT) I). (A) replication. Besides Wolbachia, research made rapid recently 1A,B). field-caught sinensis berghei modulating genes [23.Bai L. al.A gut symbiotic marcescens renders activation immune responses.Front. 1580Crossref interact [24.Cappelli al.Asaia activates eliciting anti-Plasmodium response: control.Front. Genet. 836Crossref (20) Symbionts not interfere pathogens, they directly. secretes antimalarial lipase kills at stages, effectively preventing [25.Gao via secretion lipase.Nat. 6: 806-817Crossref (6) [26.Wu P. commensal promotes permissiveness arboviruses.Cell 25: 101-112Abstract (76) Likewise, inhibits midgut growth displays entomopathogenic larvae adults. Romidepsin might metabolite [27.Saraiva R.G. al.Chromobacterium spp. mediate histone deacetylase inhibitor romidepsin.Sci. Rep. 8: 6176Crossref (23) Scholar]; aminopeptidase interferes attachment degradation Flavivirus E [28.Saraiva al.Aminopeptidase secreted sp. Panama degrading protein.PLoS Negl. Trop. Dis. 12e0006443Crossref fungi yeast killer strong developmental stages [29.Cappelli al.Killer yeasts exert anti-plasmodial activities mice.Parasite Vector. 329Crossref Toll/Jak-Stat pathways reduces [30.Dong Y. fungus activate toll JAK-STAT pathway-controlled anti-dengue aegypti.Insect Biochem. Mol. 2012; 42: 126-132Crossref (80) albopictus [31.Deng S.Q. al.Beauveria virus.J. Pest. Sci. 92: 781-789Crossref (8) Beyond fungi, (ISVs) arboviruses. Cell (CFAV) recognized ISV cells phenotype [32.Stollar V. Thomas V.L. An line (Peleg) causes fusion cells.Virology. 1975; 64: 367-377Crossref (167) Another ISV, Eilat (EILV), titers delay replication vitro. When were EILV, dissemination delayed heterologous interference mechanism [33.Nasar F. al.Eilat homologous interference.Am. J. Hyg. 2015; 93: 226Google Co-infection ISVs CFAV co-infection [34.Schultz M.J. al.Dual specific limits 518: 406-413Crossref (46) another discovered [35.Vasilakis N. al.Negevirus: taxon wide geographic distribution.J. Virol. 87: 2475-2488Crossref (121) cells, Mayaro [36.Patterson E.I. al.Negeviruses alphaviruses during coinfection.J. 95e0043321Crossref (2) All suggest arboviruses superinfection exclusion, needs further testing population. producing immunomodulatory molecules (termed paratransgenesis) 1C,D). After engineered, it reintroduced arthropod its There requirements candidate symbiont. First, should able stably horizontally maintain long enough [37.Wilke A.B. Marrelli M.T. Paratransgenesis: strategy control.Parasit. Vectors. 342Crossref (97) Second, easily culturable manipulatable, while reducing fitness [38.Lovett B. al.Transgenic rapidly malaria-endemic region Burkina Faso.Science. 364: 894-897Crossref (40) Third, target [39.Wang al.Driving bacteria.Science. 2017; 357: 1399-1402Crossref (116) several candidates shown attributes paratransgenesis (AS1), isolated ovaries, horizontally, facilitating Furthermore, Together, suggests bogorensis populate larval adult organs [40.Favia al.Bacteria genus associate stephensi, Asian malarial vector.Proc. Natl. Acad. U. 2007; 104: 9047-9051Crossref (296) Recently, successfully conditionally scorpine, significantly parasites, displaying reduced compared stain constitutively expressing [41.Shane J.L. al.Blood meal-induced inhibition microbiota.Nat. 4127Crossref (31) More recently, within heartworm immitis [42.Epis al.Chimeric stimulate development.Commun. 3: 105Crossref Notably, keep capability laboratory semi-field conditions Intriguingly, appear antagonistic one another. showed lower densities counterparts, removing enabled [43.Rosso al.Reduced diversity two areas invasion.Sci. 16091Crossref Scholar,44.Hughes al.Native impedes mosquitoes.Proc. 2014; 111: 12498-12503Crossref (137) Genetically pingshaense (Mp-hybrid) coluzzii had lifespans Mp-hybrid higher virulence inoculum load trial Faso Finally, modifying Negevirus, anti-CHIKV antibodies inhibited bacteria, fungus, even existing basis identified compelling genes, cifA cifB, involved induction rescue. models mechanism(s) still elucidated, expression bacterial germline recapitulate phenotype. Expression females rescues CI, intriguingly, appears coexpression cifB required [45.Beckmann J.F. deubiquitylating enzyme incompatibility.Nat. 2: 17007Crossref (181) Scholar,46.LePage D.P. al.Prophage WO Wolbachia-induced 543: 243-247Crossref (218) phenotypes replicated crosses between cif studies examining molecular accomplished flies 46.LePage 47.Shropshire J.D. al.One prophage Drosophila melanogaster.Proc. 115: 4987-4991Crossref (82) 48.Shropshire al.Evolution-guided mutagenesis proteins: identifying CifA's complex functional repertoire essential regions CifB.PLoS 16e1008794Crossref (11) recapitulated wPip (though here, CI) [49.Adams K.L. vector.Nat. M
Language: Английский
Citations
57
Parasites & Vectors, Journal Year: 2022, Volume and Issue: 15(1)
Published: Aug. 9, 2022
Abstract Aedes aegypti and albopictus are the main vectors of highly pathogenic viruses for humans, such as dengue (DENV), chikungunya (CHIKV), Zika (ZIKV), which cause febrile, hemorrhagic, neurological diseases remain a major threat to global public health. The high ecological plasticity, opportunistic feeding patterns, versatility in use urban natural breeding sites these have favored their dispersal adaptation tropical, subtropical, even temperate zones. Due lack available treatments vaccines, mosquito population control is most effective way prevent arboviral diseases. Resident microorganisms play crucial role host fitness by preventing or enhancing its vectorial ability transmit viral pathogens. High-throughput sequencing metagenomic analyses advanced our understanding composition functionality microbiota spp. Interestingly, shotgun metagenomics studies established that harbor conserved virome composed insect-specific (ISV). Although ISVs not infectious vertebrates, they can alter different phases cycle, interfering with transmission human host. Therefore, this review focuses on description Ae. susceptible infection pathogens, highlighting microbiota-virome competence potential strategies new emerging re-emerging arboviruses. Graphical
Language: Английский
Citations
54
The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 823, P. 153694 - 153694
Published: Feb. 8, 2022
Language: Английский
Citations
42
Protein & Cell, Journal Year: 2023, Volume and Issue: 14(10), P. 743 - 761
Published: April 25, 2023
Abstract Mosquito-borne diseases present a significant threat to human health, with the possibility of outbreaks new mosquito-borne always looming. Unfortunately, current measures combat these such as vaccines and drugs are often either unavailable or ineffective. However, recent studies on microbiomes may reveal promising strategies fight diseases. In this review, we examine advances in our understanding effects both mosquito vertebrate We argue that microbiome can have direct indirect impacts transmission diseases, symbiotic microorganisms, particularly Wolbachia bacteria, showing potential for controlling Moreover, skin vertebrates plays role preferences, while gut has an impact progression humans. As researchers continue explore highlight some future directions field. Ultimately, better interplay between mosquitoes, their hosts, pathogens, mosquitoes hosts hold key preventing
Language: Английский
Citations
26
Animal Microbiome, Journal Year: 2024, Volume and Issue: 6(1)
Published: March 7, 2024
Abstract Background The mosquito microbiome is an important modulator of vector competence and vectoral capacity. Unlike the extensively studied bacterial microbiome, fungal communities in (the mycobiome) remain largely unexplored. To work towards getting improved understanding fungi associated with mosquitoes, we sequenced mycobiome three field-collected laboratory-reared species ( Aedes albopictus, aegypti, Culex quinquefasciatus ). Results Our analysis showed both environment host were contributing to diversity mosquitoes. When comparing species, Ae. albopictus possessed a higher number diverse taxa than Cx. quinquefasciatus, while strikingly less 1% reads from aegypti samples . Fungal < even after inhibiting amplification using PNA blocker, indicating that this lacked significant was amplified sequencing approach. Using mono-association infection model, confirmed mosquito-derived isolates colonize mosquitoes support growth development at comparable rates their counterparts. Strikingly, native isolated impeded colonization symbiotic suggesting interkingdom interactions shape communities. Conclusion Collectively, study adds our different these microbes development, highlights microbial underpin medically relevent species.
Language: Английский
Citations
9
Journal of Medical Entomology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 20, 2025
Abstract Comparative studies of the microbiota in whole-body mosquitoes from natural populations and laboratory-reared specimens are scarce, particularly tropical countries like Colombia, where understanding microbial patterns is critical for effective disease control vector management. This study examines bacterial Aedes aegypti by comparing field-collected 3 Colombian regions (Southern Amazon, Central Andean region, Northern Caribbean coast) with laboratory strains (Rockefeller, Otanche, Tolima). These highly endemic dengue associated lineage 1 Ae. aegypti, known its elevated competence. Using next-generation sequencing 16S rRNA gene Illumina technology, we found that exhibited significantly higher alpha beta diversity compared to specimens. Field were enriched families such as Acetobacteraceae, Lactobacillaceae, Bacillaceae, while showed a greater abundance Enterobacteriaceae. Differential analysis revealed Acetobacter Bacillus predominated field mosquitoes, whereas Asaia, Cedacea, Chryseobacterium more common Notably, Pseudomonas Acinetobacter consistently abundant across all samples. Our findings suggest environmental factors, habitat diet, influence composition Colombia. Future research should further explore how these alongside genetic components, shape mosquito–microbiota interactions their implications transmission
Language: Английский
Citations
1
Medical and Veterinary Entomology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 28, 2025
Abstract Culicoides oxystoma Kieffer (Diptera: Ceratopogonidae) transmits many pathogens, including seven viruses, four protozoa and one nematode. This species has a wide distribution range across northern Afro‐tropical, Palearctic, Australian, Indo‐Malayan realms with broad host spectrum, cattle, buffaloes, sheep, pigs, dogs, horses even humans. The heterogeneous nature of ' blood‐feeding patterns is well documented, but the influence various blood meal sources on gut bacterial composition remains scant. Adult midges were collected during April (2023) by operating UV light traps in buffalo sheds poultry farm Purulia (India). Besides C. , eleven farm, which are vectors. liui Wirth Hubert thurmanae reported from India for first time. In all sheds, engorged females ubiquitous. Identification culturable bacteria done through polymerase chain reaction (PCR)‐based method. Blood analysis confirmed following hosts: buffaloes caught showed positive results humans not birds. Among bacteria, Bacillus cereus was abundant females. paramycoides Enterococcus faecium identified feeding cattle buffaloes' blood, while Alcaligenes faecalis found contents that fed human blood. exhibited alpha haemolytic activity. contrast, sp., B. flexus licheniformis thuringiensis E . Paenibacillus sp. Pseudomonas beta haemolysis. report particular emphasis different meals. pathogenic A. within could potentially impact pathogen acquisition increase probability their zoonotic transmissibility.
Language: Английский
Citations
1
Entomologia Experimentalis et Applicata, Journal Year: 2025, Volume and Issue: unknown
Published: April 5, 2025
Abstract Aedes aegypti (L.) and albopictus (Skuse) are important vectors that transmit arboviruses such as Dengue virus (DENV), Yellow fever (YFV), Chikungunya (CHIKV), Zika (ZIKV). Focusing on the unique characteristics of their eggs to withstand dry conditions, this study aimed elucidate viability dry‐stored subsequent morphometric changes in larvae adult mosquitoes upon rehydration. Experiments were carried out using ovitrap‐collected eggs, which reared laboratory until emergence. The resulting fed cattle blood sugar solution allowed lay paper strips, air‐dried stored for 6 months. these was tested monthly. head length total fourth‐instar head, thorax, abdomen, wing length, width, adults measured, including days needed results imply prolonging egg storage period led a decline viability, delayed emergence adults, negative impact morphometrics both Ae. . Nonetheless, did not exhibit any significant differences compared those fresh eggs.
Language: Английский
Citations
1
Microbiome, Journal Year: 2022, Volume and Issue: 10(1)
Published: April 11, 2022
Abstract Background Mosquitoes harbor microbial communities that play important roles in their growth, survival, reproduction, and ability to transmit human pathogens. Microbiome transplantation approaches are often used study host-microbe interactions identify taxa assemblages associated with health or disease. However, no such have been developed manipulate the microbiota of mosquitoes. Results Here, we an approach transfer entire between mosquito cohorts. We undertook transfers ( Culex quinquefasciatus Aedes aegypti ) within Ae. species validate determine number mosquitoes required prepare donor microbiota. After transfer, monitored development dynamics throughout life cycle. Typical holometabolous lifestyle-related structures were observed, higher larval stages, including water, less diversity adults. Microbiota recipient adults was also more similar Conclusions This provides first evidence for successful microbiome Our results highlight value methods studying mosquito-microbe lay foundation future studies elucidate factors underlying acquisition, assembly, function under controlled conditions.
Language: Английский
Citations
27