Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: March 27, 2023
Profiling
tumors
at
single-cell
resolution
provides
an
opportunity
to
understand
complexities
underpinning
lymph-node
metastases
in
head
and
neck
squamous-cell
carcinoma.
Single-cell
RNAseq
(scRNAseq)
analysis
of
cancer-cell
trajectories
identifies
a
subpopulation
pre-metastatic
cells,
driven
by
actionable
pathways
including
AXL
AURK.
Blocking
these
two
proteins
blunts
tumor
invasion
patient-derived
cultures.
Furthermore,
scRNAseq
analyses
tumor-infiltrating
CD8
+
T-lymphocytes
show
distinct
T-cell
dysfunction,
corroborated
their
clonal
architecture
based
on
receptor
sequencing.
By
determining
key
modulators
trajectories,
followed
validation
using
external
datasets
functional
experiments,
we
uncover
role
for
SOX4
mediating
exhaustion.
Finally,
interactome
between
cells
putative
the
Midkine
pathway
immune-modulation
this
is
confirmed
from
humanized
mice.
Aside
specific
findings,
study
demonstrates
importance
heterogeneity
identifying
vulnerabilities
during
early
metastasis.
Cancers,
Journal Year:
2021,
Volume and Issue:
13(8), P. 1882 - 1882
Published: April 14, 2021
In
cancer
cells,
a
vital
cellular
process
during
metastasis
is
the
transformation
of
epithelial
cells
towards
motile
mesenchymal
called
to
transition
(EMT).
The
cytoskeleton
an
active
network
three
intracellular
filaments:
actin
cytoskeleton,
microtubules,
and
intermediate
filaments.
These
filaments
play
central
role
in
structural
design
cell
behavior
are
necessary
for
EMT.
During
EMT,
undergo
as
manifested
by
elongation,
migration,
invasion,
coordinated
reorganization.
extremely
dynamic
structure,
controlled
balance
assembly
disassembly
Actin-binding
proteins
regulate
polymerization
depolymerization.
Microtubule
reorganization
also
plays
important
migration
polarization.
Intermediate
rearranged,
switching
vimentin-rich
network,
this
protein
used
marker
cell.
Hence,
targeting
EMT
regulating
activities
their
key
components
may
be
potential
solution
metastasis.
This
review
summarizes
research
done
on
physiological
functions
its
process,
effect
multidrug-resistant
(MDR)
cells—highlight
some
future
perspectives
therapy
cytoskeleton.
Cancer Cell International,
Journal Year:
2022,
Volume and Issue:
22(1)
Published: April 29, 2022
Cancer-associated
fibroblasts
(CAFs)
are
critical
components
of
the
tumor
microenvironment
(TME)
with
diverse
functions
such
as
extracellular
matrix
(ECM)
remodeling,
modulation
metabolism
and
angiogenesis,
crosstalk
both
cancer
cells
infiltrating
immune
by
production
growth
factors,
cytokines,
chemokines.
Within
TME
milieu,
CAFs
exhibit
morphological
functional
transitions
relatively
specific
markers
hold
tremendous
potential
to
facilitate
tumorigenesis,
development,
resistance
towards
multiple
therapeutic
strategies
including
chemotherapy,
radiotherapy,
targeted
therapy,
anti-angiogenesis
immunotherapy,
endocrine
therapy.
Accordingly,
themselves
downstream
effectors
and/or
signaling
pathways
targets
for
optimizing
sensitivity
anti-cancer
therapies.
This
review
aims
provide
a
detailed
landscape
role
that
play
in
conferring
different
cancers
underlying
mechanisms.
The
translational
perspectives
individualized
treatment
malignant
tumors
also
discussed.
Cell Proliferation,
Journal Year:
2023,
Volume and Issue:
56(6)
Published: Feb. 19, 2023
Epithelial-mesenchymal
transition
(EMT)
or
mesenchymal-epithelial
(MET)
plays
critical
roles
in
cancer
metastasis.
Recent
studies,
especially
those
based
on
single-cell
sequencing,
have
revealed
that
EMT
is
not
a
binary
process,
but
heterogeneous
and
dynamic
disposition
with
intermediary
partial
states.
Multiple
double-negative
feedback
loops
involved
by
EMT-related
transcription
factors
(EMT-TFs)
been
identified.
These
between
drivers
MET
finely
regulate
the
state
of
cell.
In
this
review,
general
characteristics,
biomarkers
molecular
mechanisms
different
states
were
summarized.
We
additionally
discussed
direct
indirect
tumour
More
importantly,
article
provides
evidence
heterogeneity
closely
related
to
poor
prognosis
gastric
cancer.
Notably,
seesaw
model
was
proposed
explain
how
cells
themselves
remain
specific
states,
including
epithelial
state,
hybrid/intermediate
mesenchymal
state.
Additionally,
also
review
current
status,
limitations
future
perspectives
signalling
clinical
applications.
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: March 27, 2023
Profiling
tumors
at
single-cell
resolution
provides
an
opportunity
to
understand
complexities
underpinning
lymph-node
metastases
in
head
and
neck
squamous-cell
carcinoma.
Single-cell
RNAseq
(scRNAseq)
analysis
of
cancer-cell
trajectories
identifies
a
subpopulation
pre-metastatic
cells,
driven
by
actionable
pathways
including
AXL
AURK.
Blocking
these
two
proteins
blunts
tumor
invasion
patient-derived
cultures.
Furthermore,
scRNAseq
analyses
tumor-infiltrating
CD8
+
T-lymphocytes
show
distinct
T-cell
dysfunction,
corroborated
their
clonal
architecture
based
on
receptor
sequencing.
By
determining
key
modulators
trajectories,
followed
validation
using
external
datasets
functional
experiments,
we
uncover
role
for
SOX4
mediating
exhaustion.
Finally,
interactome
between
cells
putative
the
Midkine
pathway
immune-modulation
this
is
confirmed
from
humanized
mice.
Aside
specific
findings,
study
demonstrates
importance
heterogeneity
identifying
vulnerabilities
during
early
metastasis.
