International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(14), P. 7959 - 7959
Published: July 21, 2024
Esophageal
cancer
ranks
the
seventh
in
incidence
and
sixth
death.
squamous
cell
carcinoma
(ESCC)
accounts
for
approximately
90%
of
total
cases
esophageal
cancer.
Chemotherapy
is
most
effective
drug-based
method
treatment
However,
severe
side
effects
traditional
chemotherapy
limit
its
efficacy.
Targeted
can
deliver
chemotherapeutic
drugs
to
cells
specifically
kill
these
with
reduced
effects.
In
work,
bivalent
aptamer-DNA
carrier
(BAD)
was
designed
by
using
an
ESCC
cell-specific
aptamer
as
recognition
molecule
a
GC
base-rich
DNA
sequence
drug
carrier.
With
doxorubicin
(Dox)
drugs,
aptamer-DNA-Dox
conjugate
(BADD)
constructed
targeted
killing
cells.
Firstly,
truncated
A2(35)
retained
binding
ability
obtained
through
optimization
intact
A2(80)
used
fuse
sequences
constructing
BAD
simple
hybridization.
The
results
gel
electrophoresis
flow
cytometry
analysis
showed
that
successfully
had
stronger
affinity
than
monovalent
A2(35).
Then,
loaded
Dox
construct
BADD
noncovalent
intercalation.
fluorescence
spectra
assays
bind
target
strongly.
Confocal
imaging
further
displayed
be
internalized
into
release
Dox.
CCK-8
assays,
Calcein
AM/PI
staining,
wound
healing
demonstrated
cells,
but
not
control
Our
demonstrate
developed
selectively
offering
potentially
strategy
ESCC.
Many
ailments
can
be
diagnosed
while
they
are
asymptomatic,
meaning
that
the
patient
has
no
signs
or
symptoms
of
a
progressing
disease.
If
caught
in
their
initial
stage
formation,
these
maladies
effectively
treated,
leading
to
successful
outcomes;
curative
therapies
halt
illnesses
from
advancing,
thus
improving
quality
life,
and
long-term
survival
patient.
Still
cutting-edge
upgrades
precision
technologies
necessary
for
early,
reliable,
affordable,
rapid
disease
detection,
but
also
vital
well-being
people
future
global
public
health.
The
emerging
role
utility
non-invasive
repeatable
diagnostic
test
approaches
detection
health
conditions
been
liquid
biopsies
based
on
genomic
biomarkers.
As
such,
biological
fluids
permit
any
measurable
molecular
indicator
signature
(e.g.,
proteins),
provide
valuable
information
individual’s
wellness
and/or
Among
bodily
secretions
used
diagnostics
is
sputum,
complex
viscous
gel-like
biopolymeric
network,
gained
growing
recognition
as
rich
source
biomarkers
airway
infections,
pulmonary
diseases,
serves
determinant
reveal
other
illnesses.
per
World
Health
Organization,
burden
respiratory
exacerbated
by
factors,
ranging
considerable
subjection
air
pollution
occupational
contaminants,
tobacco
smoking
second-hand
smoke,
addition
poor
socio-economic
status.
Due
likely
increase
determinants,
tract
rise,
affecting
many
individuals,
putting
stress
healthcare
facilities
services
worldwide.
We
therefore
highlight
need
use
expectorated
induced
sputum
specimens
routine
sample
testing
protein
diagnose
chronic
maladies,
predict
inflammation
progression,
well
monitor
effectiveness
treatments.
Further,
we
discuss
urgency
fast
reliable
point-of-care
methods
detect
quantify
crucial
specimens,
limitations
faced
when
dealing
with
matrices.
Journal of The Electrochemical Society,
Journal Year:
2024,
Volume and Issue:
171(3), P. 037520 - 037520
Published: March 8, 2024
The
fields
of
biosensing
have
been
transformed
by
the
discovery
extraordinary
molecular
recognition
components,
such
as
aptamers
and
biomimetic
receptors.
Systematic
Evolution
Ligands
Exponential
Enrichment
(SELEX)
is
a
method
used
to
select
aptamers,
or
short
sequences
single-stranded
DNA
(ssDNA)
RNA
(ssRNA),
based
on
their
unique
binding
affinity
target
molecules.
Molecularly
imprinted
polymers
(MIPs)
are
type
receptor
that
mimics
selectivity
natural
receptors
inside
synthetic
matrix.
They
make
it
possible
identify
pathogens,
illness
biomarkers
with
accuracy.
Aptamers
play
crucial
roles
in
various
including
diagnostics,
therapeutics,
biosensing.
Their
high
specificity,
versatility,
adaptability
enable
targeted
detection,
drug
delivery,
biomolecule
manipulation,
thereby
contributing
advancements
personalized
medicine,
biotechnology,
disease
diagnosis.
combined
cutting-edge
technologies,
like
nanotechnology
lab-on-a-chip
systems,
create
biosensors
quick,
portable,
extremely
sensitive.
These
features
anticipated
become
more
important
technology
develops,
helping
address
global
issues,
advance
capabilities,
raise
people’s
standard
living
everywhere.
Recent
innovation
Biomimetic
Receptors
Biosensing
discussed
this
review
article.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(14), P. 7959 - 7959
Published: July 21, 2024
Esophageal
cancer
ranks
the
seventh
in
incidence
and
sixth
death.
squamous
cell
carcinoma
(ESCC)
accounts
for
approximately
90%
of
total
cases
esophageal
cancer.
Chemotherapy
is
most
effective
drug-based
method
treatment
However,
severe
side
effects
traditional
chemotherapy
limit
its
efficacy.
Targeted
can
deliver
chemotherapeutic
drugs
to
cells
specifically
kill
these
with
reduced
effects.
In
work,
bivalent
aptamer-DNA
carrier
(BAD)
was
designed
by
using
an
ESCC
cell-specific
aptamer
as
recognition
molecule
a
GC
base-rich
DNA
sequence
drug
carrier.
With
doxorubicin
(Dox)
drugs,
aptamer-DNA-Dox
conjugate
(BADD)
constructed
targeted
killing
cells.
Firstly,
truncated
A2(35)
retained
binding
ability
obtained
through
optimization
intact
A2(80)
used
fuse
sequences
constructing
BAD
simple
hybridization.
The
results
gel
electrophoresis
flow
cytometry
analysis
showed
that
successfully
had
stronger
affinity
than
monovalent
A2(35).
Then,
loaded
Dox
construct
BADD
noncovalent
intercalation.
fluorescence
spectra
assays
bind
target
strongly.
Confocal
imaging
further
displayed
be
internalized
into
release
Dox.
CCK-8
assays,
Calcein
AM/PI
staining,
wound
healing
demonstrated
cells,
but
not
control
Our
demonstrate
developed
selectively
offering
potentially
strategy
ESCC.
