Black Phosphorus-Loaded Gelatin Methacryloyl Hydrogels Enhance Angiogenesis via Activation of the PEAK1–MAPK Pathway DOI

Ruoting Xian,

Hongyi Xian,

Hao Dong

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Repair and regeneration of oral maxillofacial tissue defects remain significant challenges, mainly due to the limitations existing treatment approaches. Conventional methods such as transplantation, scaffolds, growth factors, stem cell therapies often face obstacles, including donor shortages, insufficient vascularization, safety concerns. There is an urgent need for innovative therapeutic strategies effectively promote vascular while minimizing complications. Black phosphorus nanosheets (BPNSs) hydrogels present advantages broad application potential carriers their biocompatibility, degradability, controlled drug release properties. By combining various characterization techniques detection methods, we conducted a thorough analysis BPNSs gelatin methacryloyl (GelMA) scaffolds loaded with (BP-GelMA). The results indicate that this study successfully prepared uniform size, good dispersion, intact structure. Moreover, BP-GelMA composite demonstrated excellent swelling behavior structural stability enabling BPNSs. This investigated angiogenic effects at concentrations 0, 12.5, 25.0 μg/mL. In vitro experiments showed significantly enhanced endothelial proliferation, migration, tube formation. vivo 12.5 μg/mL did not induce developmental toxicity in zebrafish promoted neovascularization. RNA-Seq revealed activates angiogenesis-related biological processes. Mechanistic studies identified PEAK1 central regulator, driving formation through activation MAPK signaling pathway. These findings highlight strategy promoting angiogenesis underscore importance optimizing achieve maximum efficacy clinical applications.

Language: Английский

ANXA2 promotes chondrocyte differentiation and fracture healing by regulating the phosphorylation of STAT3 and PI3K/AKT signaling pathways DOI
Xinru Wang, Wei Dong, Xinyi Wang

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111617 - 111617

Published: Jan. 1, 2025

Language: Английский

Citations

0
Polylactic acid electrospun membranes coated with chiral hierarchical-structured hydroxyapatite nanoplates promote tendon healing based on a macrophage-homeostatic modulation strategy DOI
Gang Luo, Juehong Li, Shuai Chen

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 47, P. 460 - 480

Published: Feb. 13, 2025

Language: Английский

Citations

0
Tβ4-Exosome-loaded Hemostatic and Antibacterial Hydrogel to Improve Vascular Regeneration and Modulate Macrophage Polarization for Diabetic Wound Treatment DOI Creative Commons
Hua Yu, Bin Wang, Zihao Li

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101585 - 101585

Published: Feb. 18, 2025

Diabetic wounds often exhibit delayed healing due to compromised vascular function and intensified inflammation. In this study, we overexpressed Thymosin β4 (Tβ4) in Adipose-Derived Stem Cells (ADSCs) produce Exosomes (Exos) rich Tβ4. We then utilized a dual photopolymerizable hydrogel composed of Hyaluronic Acid Methacryloyl (HAMA) Poly-L-lysine (PLMA) for the sustained release Tβ4-Exos on diabetic wounds. The results showed that could stimulate angiogenesis collagen synthesis, mitigate inflammation by promoting polarization M1-type macrophages inhibiting M2-type macrophages. Furthermore, was found activate PI3K/AKT/mTOR/HIF-1a signaling pathway, thereby enhancing proliferation. summary, HAMA-PLMA (HP) management through upregulation HIF-1a pathway modulation macrophage proliferation significantly accelerated process

Language: Английский

Citations

0
Neuralized and vascularized fast bone regeneration using recombinant humanized type 1 collagen and native bone composite inorganic salts DOI Creative Commons
Peng Hou, Song Chen, Tuerxun Maimaitiaili

et al.

APL Materials, Journal Year: 2025, Volume and Issue: 13(3)

Published: March 1, 2025

Bone injury is a prevalent condition in clinical therapy that can lead to significant functional impairments and substantially disrupt the quality of life for patients. However, there has been limited breakthrough achieving neuralized vascularized rapid bone regeneration. In this study, we collaborated with recombinant humanized collagen 1 (rhCOL1), native composite inorganic salts (NBCISs), methacrylated silk fibroin (SilMA), marrow mesenchymal stem cells (BMSCs) construct biomimetic organic bio-mineralized multifunctional organoids repair defects, regeneration within just six weeks rabbits. We first determined optimal concentration SilMA (10%) by comprehensively evaluating crosslinking, operability, BMSC proliferation. The rhCOL1 NBCIS mixture was prepared using ratio 3:7, reference bone, subsequently added create biomineralized microenvironments NCSilMA. Similarly, proportions were optimized based on their effects compressive modulus, swelling, degradation. As result, successfully constructed hydrogel scaffold defect repair, characterized excellent biodegradability, appropriate strength, good biocompatibility, osteoinductive biological function. Finally, BMSC-loaded NCSilMA (organoids) achieved regeneration, up-regulated osteogenic genes enhanced cell colonization, collagen, polysaccharide deposition.

Language: Английский

Citations

0
PTEN/PI3K/AKT pathway activation with hypoxia-induced human umbilical vein endothelial cell exosome for angiogenesis-based diabetic skin reconstruction DOI Creative Commons
Z Y Li,

Yuhao Bai,

Hao Wu

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101651 - 101651

Published: March 1, 2025

Diabetic skin, a major clinical challenge due to impaired wound healing, is often exacerbated by hypoxic microenvironment at the site. Exosomes have been proven excellent biological activities and applied solve many bioengineering problems. However, wide application of exosomes still limited their short in vitro lifetime low yield. To overcome these limitations, this study specifically enhances pro-angiogenic efficacy through treatment achieves sustained release using hydrogel loading. In vitro, hypoxia-induced (Hp-Exo) significantly enhanced endothelial cell migration, proliferation, angiogenic capacity. vivo, Gelman hydrogels loaded with Hp-Exo accelerated closure, promoted collagen deposition, increased vascularization diabetic mice. miRNA sequencing revealed that induced under conditions contain various miRNAs, which enhance vascular angiogenesis PTEN/PI3K/AKT pathway. These results highlight exosomes, when delivered biocompatible platform, provide potential therapeutic approach improve healing stimulating tissue regeneration.

Language: Английский

Citations

0
Milk-Derived Exosomes as Functional Nanocarriers in Wound Healing: Mechanisms, Applications, and Future Directions DOI Creative Commons

Jing Ruan,

Yuping Xia,

Yilei Ma

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101715 - 101715

Published: March 1, 2025

Language: Английский

Citations

0
Unveiling the synergistic potential: Surface topology and osteogenic elements in biodegradable Zn alloys DOI
Xuan Yang,

Huafang Li

Surface and Coatings Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132140 - 132140

Published: April 1, 2025

Language: Английский

Citations

0
Black Phosphorus-Loaded Gelatin Methacryloyl Hydrogels Enhance Angiogenesis via Activation of the PEAK1–MAPK Pathway DOI

Ruoting Xian,

Hongyi Xian,

Hao Dong

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Repair and regeneration of oral maxillofacial tissue defects remain significant challenges, mainly due to the limitations existing treatment approaches. Conventional methods such as transplantation, scaffolds, growth factors, stem cell therapies often face obstacles, including donor shortages, insufficient vascularization, safety concerns. There is an urgent need for innovative therapeutic strategies effectively promote vascular while minimizing complications. Black phosphorus nanosheets (BPNSs) hydrogels present advantages broad application potential carriers their biocompatibility, degradability, controlled drug release properties. By combining various characterization techniques detection methods, we conducted a thorough analysis BPNSs gelatin methacryloyl (GelMA) scaffolds loaded with (BP-GelMA). The results indicate that this study successfully prepared uniform size, good dispersion, intact structure. Moreover, BP-GelMA composite demonstrated excellent swelling behavior structural stability enabling BPNSs. This investigated angiogenic effects at concentrations 0, 12.5, 25.0 μg/mL. In vitro experiments showed significantly enhanced endothelial proliferation, migration, tube formation. vivo 12.5 μg/mL did not induce developmental toxicity in zebrafish promoted neovascularization. RNA-Seq revealed activates angiogenesis-related biological processes. Mechanistic studies identified PEAK1 central regulator, driving formation through activation MAPK signaling pathway. These findings highlight strategy promoting angiogenesis underscore importance optimizing achieve maximum efficacy clinical applications.

Language: Английский

Citations

0