Microplastics magnify inhibitive effects of perfluorooctanoic acid on the marine microbial loop

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121223 - 121223

Published: Feb. 1, 2025

Language: Английский

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Metagenomic community composition and resistome analysis in a full-scale cold climate wastewater treatment plant

Environmental Microbiome, Journal Year: 2022, Volume and Issue: 17(1)

Published: Jan. 15, 2022

Abstract Background Wastewater treatment plants are an essential part of maintaining the health and safety general public. However, they also anthropogenic source antibiotic resistance genes. In this study, we characterized resistome, distribution classes 1–3 integron-integrase genes ( intI1, intI2, intI3 ) as mobile genetic element biomarkers, bacterial phage community compositions in North End Sewage Treatment Plant Winnipeg, Manitoba. Samples were collected from raw sewage, returned activated sludge, final effluent, dewatered sludge. A total 28 viral metagenomes sequenced over two seasons, fall winter. Integron-integrase genes, 16S rRNA gene, coliform beta-glucuronidase gene quantified during time period. Results Bacterial observed above 1% relative abundance all treatments Actinobacteria (39.24% ± 0.25%), Beta-proteobacteria (23.99% 0.16%), Gamma-proteobacteria (11.06% 0.09%), Alpha-proteobacteria (9.18 0.04%). Families within Caudovirales order: Siphoviridae (48.69% 0.10%), Podoviridae 0.07%), Myoviridae (19.94% 0.09%) dominant throughout NESTP. The most abundant genera (in terms average percent abundance) influent, respectively, includes Mycobacterium (37.4%, 18.3%, 46.1%, 7.7%), Acidovorax (8.9%, 10.8%, 5.4%, 1.3%), Polaromonas (2.5%, 3.3%, 1.4%, 0.4%). class samples was tetracycline (17.86% 0.03%) followed by peptide antibiotics (14.24% 0.03%), macrolides (10.63% 0.02%). Similarly, contained a higher prevalence macrolide (30.12% 0.30%), (10.78% 0.13%), (8.69% 0.11%) resistance. addition, intI1 (1.14 × 10 4 copies/mL) (4.97 3 while intI2 remained low (6.4 1 copies/mL). Conclusions successfully reduced bacteria, DNA although many effluent biosolids. presence suggests that could be actively disseminating between both environmental pathogenic bacteria.

Language: Английский

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Inherent tendency of Synechococcus and heterotrophic bacteria for mutualism on long-term coexistence despite environmental interference

Science Advances, Journal Year: 2022, Volume and Issue: 8(39)

Published: Sept. 30, 2022

Mutualism between Synechococcus and heterotrophic bacteria has been found to support their prolonged survival in nutrient-depleted conditions. However, environmental interference on the fate of mutualism is not understood. Here, we show that exogenous nutrients disrupt established mutualism. Once were exhausted, gradually reestablished metabolic during 450 days culture, which revived unhealthy cells. Using metagenomics, metatranscriptomics, 15N tracer method, reveal associated bacterial nitrogen fixation triggered reestablishment revival health. During this process, community structure functions underwent tremendous adjustments achieve driving effect, a cogeneration nitrogen, phosphorus, iron, vitamin by sustained Synechococcus's healthy growth. Our findings suggest may have an inherent tendency toward despite interference. This exhibit coevolutionary adaptations nutrient-deficient environments.

Language: Английский

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Phytoplankton Producer Species and Transformation of Released Compounds over Time Define Bacterial Communities following Phytoplankton Dissolved Organic Matter Pulses

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(7)

Published: July 6, 2023

Phytoplankton-bacterium interactions are mediated, in part, by phytoplankton-released dissolved organic matter (DOMp). Two factors that shape the bacterial community accompanying phytoplankton (i) producer species, defining initial composition of released DOMp, and (ii) DOMp transformation over time. We added from diatom Skeletonema marinoi cyanobacterium Prochlorococcus marinus MIT9312 to natural communities eastern Mediterranean determined responses a time course 72 h terms cell numbers, production, alkaline phosphatase activity, changes active based on rRNA amplicon sequencing. Both types were demonstrated serve as carbon and, potentially, phosphorus sources. Bacterial diatom-derived DOM treatments maintained higher Shannon diversities throughout experiment yielded production lower activity compared cyanobacterium-derived after 24 incubation (but not 48 h), indicating greater usability DOM. significantly differed between well different times, pointing certain specificity for successive utilization taxa The highest differences with occurred shortly additions, suggesting high toward highly bioavailable compounds. conclude phytoplankton-associated strongly shaped its

Language: Английский

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Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Aug. 17, 2022

Underexplored seawater environments may contain biological resources with potential for new biotechnological applications. Metagenomic techniques revolutionized the study of bacterial communities but culture dependent methods will still be important to help biodiscovery products and enzymes from marine bacteria. In this context, we promoted growth bacteria a rock pond by compared results independent methods. The total number diversity were studied in different agar plate media during 6 weeks. Agar counting was same order magnitude direct microscopy counts. highest efficiency cultivation 45% attained medium. Molecular analysis revealed 10 phyla which only four isolated method. On other hand, taxonomic orders detected not molecular technique. These include Bacillota Actinomycetota. Our shows that it is possible grow more than traditionally considered 1% sample using standard laboratorial conditions. also demonstrate importance approaches future

Language: Английский

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Biological interactions with Prochlorococcus: implications for the marine carbon cycle

Trends in Microbiology, Journal Year: 2023, Volume and Issue: 32(3), P. 280 - 291

Published: Sept. 17, 2023

Language: Английский

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Collaborative metabolic curation of an emerging model marine bacterium, Alteromonas macleodii ATCC 27126

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0321141 - e0321141

Published: April 24, 2025

Inferring the metabolic capabilities of an organism from its genome is a challenging process, relying on computationally-derived or manually curated networks. Manual curation can correct mistakes in draft network and add missing reactions based literature, but requires significant expertise often bottleneck for high-quality reconstructions. Here, we present synopsis community workshop model marine bacterium Alteromonas macleodii ATCC 27126 database BioCyc, focusing pathways utilizing organic carbon nitrogen sources. Due to scarcity biochemical information gene knock-outs, process relied primarily published growth phenotypes bioinformatic analyses, including comparisons with related strains. We report full utilization algal polysaccharides alginate pectin contrast inconclusive evidence one-carbon metabolism mixed acid fermentation, accordance lack methanol formate. Pathways amino degradation are ubiquitous across strains, yet enzymes threonine, tryptophan tyrosine were not identified. Nucleotide also partial 27126. postulate that demonstrated nitrate as sole source proceeds via reductase pathway hybrid known pathways. Our highlights value joint interactive efforts, shows major knowledge gaps regarding metabolism. The manually-curated reconstruction available “Tier-2” BioCyc.

Language: Английский

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Structure and Long-Term Stability of the Microbiome in Diverse Diatom Cultures

Microbiology Spectrum, Journal Year: 2021, Volume and Issue: 9(1)

Published: June 30, 2021

Microalgal cultures are often maintained in xenic conditions, i.e., with associated bacteria, and many studies indicate that these communities both complex have significant impacts on the physiology of target photoautotroph. Here, we investigated structure stability microbiomes a diverse sampling diatoms during long-term maintenance serial batch culture. We found that, counter to our initial expectation, evenness diversity increased time since cultivation, driven by decrease dominance most abundant taxa each also site from which at culture was initially collected had stronger impact microbiome than diatom species; however, some bacterial were commonly present despite having widely geographically separated collection sites. Our results support conclusion stochastic conditions (i.e., local microbial community site) important for microbiomes, but deterministic forces such as negative frequency dependence natural selection exerted work. IMPORTANCE Natural extremely complex, more species coexisting same place there different resources them. Understanding allow this high level has been central focus ecological evolutionary theory decades. used stock diatoms, years continuous growth alongside populations proxies communities. show remained relatively stable years, is evidence worked stabilize coexistence instead favoring competition exclusion. regional differences communities, globally core potentially selected cultures. interactions between bacteria basic science practical science, industrial biofuel production.

Language: Английский

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Prochlorococcus Exudate Stimulates Heterotrophic Bacterial Competition with Rival Phytoplankton for Available Nitrogen

mBio, Journal Year: 2022, Volume and Issue: 13(1)

Published: Jan. 11, 2022

In nutrient-poor habitats, competition for limited resources is thought to select organisms with an enhanced ability scavenge nutrients and utilize them efficiently. Such adaptations characterize the cyanobacterium Prochlorococcus , most abundant photosynthetic organism in nutrient-limited open ocean.

Language: Английский

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No short-term effect of sinking microplastics on heterotrophy or sediment clearing in the tropical coral Stylophora pistillata

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Jan. 27, 2022

Investigations of encounters between corals and microplastics have, to date, used particle concentrations that are several orders magnitude above environmentally relevant levels. Here we investigate whether closer values reported in tropical coral reefs affect sediment shedding heterotrophy reef-building corals. We show single-pulse microplastic deposition elicits significantly more polyp retraction than comparable amounts calcareous sediments. When deposited separately from sediments, remain longer on through stronger adhesion periods examination by the polyps. Contamination sediments with does not retard corals' clearing rates. Rather, speed-up shedding, possibly affecting its electrostatic behaviour. Heterotrophy rates three times higher ingestion when encounter microzooplankton (Artemia salina cysts) separately. Exposed cysts-microplastic combinations, feed preferentially cysts regardless concentration. Chronic-exposure experiments should test our conclusions hold true under environmental conditions typical inshore marginal reefs.

Language: Английский

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