Characterization of membrane vesicles in Alteromonas macleodii indicates potential roles in their copiotrophic lifestyle

microLife, Journal Year: 2022, Volume and Issue: 4

Published: Dec. 20, 2022

Abstract Bacterial membrane vesicles (MVs) are abundant in the oceans, but their potential functional roles remain unclear. In this study we characterized MV production and protein content of six strains Alteromonas macleodii, a cosmopolitan marine bacterium. macleodii varied rates, with some releasing up to 30 MVs per cell generation. Microscopy imaging revealed heterogenous morphologies, including aggregated within larger structures. Proteomic characterization that A. rich proteins related iron phosphate uptake, as well functions biofilm formation. Furthermore, harbored ectoenzymes, such aminopeptidases alkaline phosphatases, which comprised 20% total extracellular enzymatic activity. Our results suggest may support its growth through generation ‘hotspots’ facilitate access essential substrates. This provides an important basis decipher ecological relevance heterotrophic bacteria.

Language: Английский

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Comparative Metagenomics Provide Mechanistic Insights into the Biodegradation of the Non-hydrolysable Plastic Polyvinyl Chloride in Gut Microbiota of Insect Larvae

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 6, 2024

Abstract Using microbiomes to mitigate global plastic pollution is of paramount importance. Insect have garnered emerging interest for their ability biodegrade non-hydrolysable polymers. The larvae Spodoptera frugiperda , a globally prevalent migratory crop pest, are accidentally discovered consume polyvinyl chloride (PVC) films, highlighting the role gut microbiome. Following migration S. frugiperd in China, this study displays comprehensive geographical profile its larval microbiota and significant shift after PVC feeding. comparative metagenomics we revealed functional redundancy within two distinct insects ingestion, surprisingly potent PVC-dechlorinating activity an NADH peroxidase (6.48 mg/L chlorine produced 96 hours with as cofactor) encoded by Enterococcus casseliflavus EMBL-3. These findings open new avenue understanding biodegradation mechanism enable development biotechnologies pollution.

Language: Английский

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Landscape connection and patch complexity explain plant community similarity in sandy grasslands better than habitat amount and landscape heterogeneity via network analysis

Community Ecology, Journal Year: 2024, Volume and Issue: 25(2), P. 197 - 210

Published: April 20, 2024

Language: Английский

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Mitogenomic profiling and gut microbial analysis of the newly identified polystyrene-consuming lesser mealworm in Kenya

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 12, 2024

Language: Английский

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Draft genomes of three closely related low light-adapted Prochlorococcus

BMC Genomic Data, Journal Year: 2023, Volume and Issue: 24(1)

Published: Feb. 24, 2023

Abstract Objectives The marine cyanobacterium Prochlorococcus is a critical part of warm ocean ecosystems and model for studying microbial evolution ecology. To expand the representation this organism’s vast wild diversity in sequence collections, we performed set isolation efforts targeting low light-adapted . Three genomes resulting from larger body work are described here. Data description We present draft-quality enrichment cultures P1344, P1361, P1363, sampled North Pacific. were built Illumina paired reads assembled de novo. Supporting datasets raw reads, assessments, sequences co-enriched heterotrophic bacteria also provided. These three represent members LLIV clade that closely related, with 99.9% average nucleotide identity between pairs, yet vary gene content. Expanding powerful toolkit genomes, these provide an opportunity to study fine-scale variation microevolutionary processes.

Language: Английский

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Filter Plating Method for Rendering Picocyanobacteria Cultures Free of Heterotrophic Bacterial Contaminants and Clonal

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Feb. 16, 2022

Isolates of the marine picocyanobacteria, Prochlorococcus and Synechococcus, are often accompanied by diverse heterotrophic "contaminating" bacteria, which can act as confounding variables in otherwise controlled experiments. Traditional microbiological methods for eliminating contaminants, such direct streak-plating, unsuccessful with this particular group microorganisms. While they will grow pour plates, colonies remain contaminated bacteria that migrate through soft agar. Additionally, axenic clones picocyanobacteria be recovered via dilution-to-extinction liquid medium, but efficiency recovery is low, requiring large numbers 96-well plates. Here, we detail a simple effective protocol rendering cultures Synechococcus strains free bacterial contaminants while at same time yielding clonal isolates. We build on fact co-culture specific heterotrophs-"helper heterotrophs"-is necessary to from single cells Suspecting physical contact between helper picocyanobacterial was not "helper effect," developed embedded agar filter overlaid surface, culture diluted then spotted top filter. With approach, motile cannot swim colonies, it possible obtain expected number given input (i.e., Poisson distribution an value equal cells), thus ensuring colonies. Using protocol, rendered three two Prochlorococcus, 19 new coastal seawater bacteria. The simplicity approach should expand repertoire available physiological It also enable study microdiversity populations facilitating large-scale isolation source, including natural seawater.

Language: Английский

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Marine phytoplankton and heterotrophic bacteria rapidly adapt to future pCO₂conditions in experimental co-cultures

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 10, 2024

ABSTRACT The CO 2 content of Earth’s atmosphere is rapidly increasing due to human consumption fossil fuels. Models based on short-term culture experiments predict that major changes will occur in marine phytoplankton communities the future ocean, but these models rarely consider how evolutionary potential or interactions within microbial may influence changes. Here we experimentally evolved representatives four functional types (silicifiers, calcifiers, coastal cyanobacteria, and oligotrophic cyanobacteria) co-culture with a heterotrophic bacterium, Alteromonas , under either present-day predicted pCO conditions. Growth rates cyanobacteria generally increased both conditions, growth defects observed ancestral Prochlorococcus cultures at elevated axenic were diminished after evolution, possibly regulatory mutations antioxidant genes. Except for mutational profiles suggested experienced primarily purifying selection, most lineages showed evidence directional especially when co-cultured eukaryotic phytoplankton, where evolution appeared favor broad metabolic switch from small organic acids catabolism more complex carbon substrates. Evolved also poorer “helpers” supporting assertion interaction between bacteria not true mutualism rather competitive stabilized by Black Queen processes. This work provides new insights respond anthropogenic change mechanisms governing structure function communities.

Language: Английский

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Allochthonous Groundwater Microorganisms Affect Coastal Seawater Microbial Abundance, Activity and Diversity

Journal of Geophysical Research Biogeosciences, Journal Year: 2024, Volume and Issue: 129(2)

Published: Feb. 1, 2024

Abstract Submarine groundwater discharge (SGD) is a globally important process supplying nutrients and trace elements to the coastal environment, thus playing pivotal role in sustaining marine primary productivity. Along with nutrients, also contains allochthonous microbes that are discharged from terrestrial subsurface into sea. Currently, little known about interactions between groundwater‐borne seawater microbial populations, microbes' upon introduction populations. Here, we investigated abundance, activity diversity site strongly influenced by SGD. In addition, through laboratory‐controlled bottle incubations, mimicked different mixing scenarios seawater. Our results demonstrate addition of 0.1 μm filtered stimulated heterotrophic increased abundance compared control seawater, whereas 0.22 filtration treatments induced productivity Synechococcus growth. 16S rRNA gene sequencing showed strong shift SAR11‐rich community samples Rhodobacteraceae dominance <0.1 treatment, agreement enrichment SGD field site. These suggest delivered may affect intrinsic highlighting cryptic interplay environments, which has implications for carbon cycling.

Language: Английский

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Microbial Nanobioremediation of Micro-Nanoplastics: Current Strategies, Challenges, and Future Prospects

Published: Jan. 1, 2024

Plastic is the most common man-made polymer. It possesses greater flexibility, durability, lower manufacturing cost, and lighter weight, leading to increased consumption production of single-use plastics. In developed countries, plastic materials are used in packaging, construction, automobiles, medical devices, furniture, toys, etc. Improper disposal waste poor degradation lead massive accumulation environment. degrades naturally with help sunlight, heat, oxygen level, salinity into micro-nanoplastics (MNPs). The produced MNPs major sources pollution affecting our entire ecosystem directly or indirectly, by emitting contaminants for decades. conventional method micro-nanoplastic produces highly toxic compounds, posing environmental health risks. Bioremediation considered be a better alternative degrading MNPs. A combined research strategy required utilizing various technologies enhance remediation At present, incorporation nanotechnology, membrane technology, enzyme technology has improved bioremediation (BR) process. For instance, interaction nanoparticles such as SiO2, fullerene 60 microorganisms was found decrease lag phase increase biodegradation (BD) rate. current review focuses on comprehensive view microbial nanobioremediation (NBR) process address issues caused

Language: Английский

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Spirulina-Based Multispecies Phototrophic Biofilm Anodic Biocatalyst Endures a Prolonged Dark Phase within Light–Dark Cycle Operations and Enhances Anodic Performance in Biophotovoltaic Cells

ACS ES&T Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Phototrophs with heterotrophic bacterial consortium as an electrode biocatalyst are emerging concept for developing naturally sustained biophotovoltaic systems. Herein, Spirulina subsalsa-based mixed community anodic catalyst in a microbial fuel cell (MFC) setup ferricyanide catholyte 78 days light–dark (16–8 h) cycle-based operation was investigated. The biofilm developed inducted recalcitrant comprising Halomonas, Alcanivorax, Pelagibacterium, and Rhizobiales the major genera. In extended dark phase (9 days) within cyclic operation, sequential shift of metabolism from photosynthesis to fermentative states increased population were observed. Under direct contact graphite anode, initiated oscillating open-circuit potentials MFC response circadian trend. delivered maxima 587 μW m–2 418 (at 10 kΩ) under corresponding phases, respectively. potential shifted more negative value, reaching −415.5 mV starvation period. Analyses reaction rates (extracted Tafel plots), corrosion potential, current, polarization resistance, residual redox charges voltammograms) performed understand processes. Two peaks 0.6 V (irreversible, extracellular) 0.26 (reversible, cell-surface attached) attributed mediation this process. Additionally, catholyte-diffused interacts biofilm, getting trapped matrix polymeric structures, thus preventing sudden cytotoxic elimination cells promoting oxidative charge accumulation over its surface, improving potential. Rapid respiratory oxygen consumption, biofilm's structural reorganization, ferricyanide's chemical speciation inside primary factors that govern performance biofuel during prolonged operations. critical findings unveiled through study advance our understanding resilience phototroph-based multispecies catalysts devices long-term

Language: Английский

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