Organic Connection of Holobiont Components and the Essential Roles of Core Microbes in the Holobiont Formation of Feral Brassica napus DOI Creative Commons
Seong-Jun Chun,

Yingshun Cui,

Su‐Hyang Yoo

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: July 8, 2022

Brassica napus (Rapeseed) is an econfomically important oil-producing crop. The microbial interactions in the plant holobiont are fundamental to understanding of growth and health. To investigate dynamics feral B. napus, a total 215 samples, comprised bulk soil, primary root, lateral dead leaf, caulosphere, basal apical carposphere, anthosphere, were collected from five different grassland sites South Korea. soil properties differed sampling sites, but prokaryotic communities segregated according components. structures site-specific SparCC networks similar across regions. Recurrent patterns found holobionts recurrent network. Ralstonia sp., Massilia Rhizobium clusters observed consistently identified as core taxa phyllosphere, leaf microbiome, rhizosphere, respectively. Arthropod-related microbes, such Wolbachia Gilliamella Corynebacteriales amplicon sequence variants, anthosphere. PICRUSt2 analysis revealed that microbes also possessed specific functions related components, degradation pathways microbiome. Structural equation modeling showed organic connections among components essential roles formations natural ecosystem. Microbes coexisting relatively stable community structures, even though regions characteristics different. each component organically connected form their own holobiont. In addition, plant-related especially holobiont, interaction survival coexistence ecosystems.

Language: Английский

Soil-dependent fate of Klebsiella pneumoniae and Listeria monocytogenes after incorporation of digestates in soil microcosms DOI Creative Commons

Sophie Michel-Le Roux,

Christine Ziebal,

Gaëtan Pinsard

et al.

Applied Soil Ecology, Journal Year: 2025, Volume and Issue: 208, P. 105965 - 105965

Published: Feb. 20, 2025

Language: Английский

Citations

0
Biochar amendment modulates root metabolome and rhizosphere microbiome of wheat DOI Creative Commons

Hanyue Yang,

Patricia Kerner,

Xi Liang

et al.

Biochar, Journal Year: 2025, Volume and Issue: 7(1)

Published: March 6, 2025

Language: Английский

Citations

0
Genomic and phenotypic characterization of a red-pigmented strain of Massilia frigida isolated from an Antarctic microbial mat DOI Creative Commons

Jacob M. C. Shaffer,

LesleyAnn Giddings, Robert M. Samples

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: May 12, 2023

The McMurdo Dry Valleys of Antarctica experience a range selective pressures, including extreme seasonal variation in temperature, water and nutrient availability, UV radiation. Microbial mats this ecosystem harbor dense concentrations biomass an otherwise desolate environment. inhabitants must mitigate these pressures via specialized enzymes, changes to the cellular envelope, production secondary metabolites, such as pigments osmoprotectants. Here, we describe isolation characterization Gram-negative, rod-shaped, motile, red-pigmented bacterium, strain DJPM01, from microbial mat within Don Juan Pond Basin Wright Valley. Analysis DJMP01’s genome indicates it can be classified member Massilia frigida species. contains several genes associated with cold salt tolerance, multiple RNA helicases, protein chaperones, cation/proton antiporters. In addition, identified 17 putative metabolite gene clusters, number nonribosomal peptides ribosomally synthesized post-translationally modified (RiPPs), among others, biosynthesis pathway for antimicrobial pigment prodigiosin. When cultivated on complex agar, prodiginines, antibiotic prodigiosin, 2-methyl-3-propyl-prodiginine, 2-methyl-3-butyl-prodiginine, 2-methyl-3-heptyl-prodiginine, cycloprodigiosin, were detected by LC–MS. Genome analyses sequenced members genus prodigiosin is unique Antarctic strains. UV-A radiation, ecological stressor Antarctic, was found significantly decrease abundance prodiginines produced DJPM01. Genomic phenotypic evidence DJPM01 respond conditions DJP mat, under conditions,

Language: Английский

Citations

8
Leaf bacterial microbiota response to flooding is controlled by plant phenology in wheat (Triticum aestivum L.) DOI Creative Commons
Davide Francioli, Geeisy Cid, Mohammad‐Reza Hajirezaei

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: July 1, 2022

Abstract Leaf microbiota mediates foliar functional traits, influences plant fitness, and contributes to various ecosystem functions, including nutrient water cycling. Plant phenology harsh environmental conditions have been described as the main determinants of leaf assembly. How climate change may modulate is unresolved thus, we a limited understanding on how stresses associated with driven weather events affect composition functions microbes inhabiting phyllosphere. Thus, conducted pot experiment determine effects flooding stress wheat microbiota. Since might be an important factor in response hydrological stress, was induced at different growth stages (tillering, booting flowering). Using metabarcoding approach, monitored bacteria flooding, while key soil traits were measured correlate physiological edaphic changes shifts bacterial In our study, stage represented driver composition, early late plants showed distinct communities. Overall, had differential effect dynamics depending which developmental it induced, more pronounced disruption community assembly observed younger plants.

Language: Английский

Citations

12
Response of the wheat mycobiota to flooding revealed substantial shifts towards plant pathogens DOI Creative Commons
Davide Francioli, Geeisy Cid, Mohammad‐Reza Hajirezaei

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 28, 2022

Rainfall extremes are intensifying as a result of climate change, leading to increased flood risk. Flooding affects above- and belowground ecosystem processes, representing substantial threat crop productivity under change. Plant-associated fungi play important roles in plant performance, but their response abnormal rain events is unresolved. Here, we established glasshouse experiment determine the effects flooding stress on spring wheat-mycobiota complex. Since phenology could be an factor hydrological stress, was induced only once at different growth stages, such tillering, booting flowering. We assessed wheat mycobiota three soil-plant compartments (phyllosphere, roots rhizosphere) using metabarcoding. Key soil traits were measured correlate physiological edaphic changes with shifts structure functional guilds. reduced fitness, caused dramatic assembly across entire plant. Notably, observed transition consisting decline mutualist abundance richness concomitant increase pathogens. Indeed, fungal pathogens associated cereal diseases, Gibberella intricans, Mycosphaerella graminicola, Typhula incarnata Olpidium brassicae significantly flooding. Overall, our study demonstrate detrimental effect complex, highlighting urgent need understand how change-associated abiotic stressors alter plant-microbe interactions crops.

Language: Английский

Citations

12
Seed bacterial microbiota in post-submergence tolerant and sensitive barley genotypes DOI Creative Commons
Eva María Gómez-Álvarez, Monique Salardi-Jost, Germán Darío Ahumada

et al.

Functional Plant Biology, Journal Year: 2024, Volume and Issue: 51(2)

Published: Jan. 24, 2024

Flooding is a predominant abiotic stress for cultivated plants, including barley. This cereal crop shows large adaptability to different environmental conditions, suggesting the presence of key traits tolerate adverse conditions. During germination, genetic variations account dissimilarities in flooding tolerance. However, differences seed microbiota may also contribute tolerance/sensitivity during seedling establishment. work investigated microbiome among grains barley accessions. Two phenotypes were compared, each either tolerant or sensitive short submergence period followed by recovery. The study used metataxonomic analysis based on 16S ribosomal RNA gene sequencing and subsequent functional prediction. Our results support hypothesis that bacterial inhabiting seeds are between accessions, which harbour specific phyla families. Finally, bacteria detected accessions show peculiar enrichment suggests possible connection with successful germination

Language: Английский

Citations

2
Nitrogen Fertilizer Type and Genotype as Drivers of P Acquisition and Rhizosphere Microbiota Assembly in Juvenile Maize Plants DOI Creative Commons

Melissa Mang,

Niels Julian Maywald, Xuelian Li

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(3), P. 544 - 544

Published: Jan. 25, 2023

Phosphorus (P) is an essential nutrient for plant growth and development, as well important factor limiting sustainable maize production. Targeted nitrogen (N) fertilization in the form of ammonium has been shown to positively affect Pi uptake under P-deficient conditions compared nitrate. Nevertheless, its profound effects on root traits, P uptake, soil microbial composition are still largely unknown. In this study, two genotypes F160 F7 with different sensitivity were used investigate phosphorus-related traits such hair length, diameter, AMF association, multiple efficiencies limitation when fertilized either or Ammonium application improved phosphorous acquisition efficiency genotype but not F160, suggesting that plays role how a particular N affects maize. Additionally, metabarcoding data showed young roots able promote distinct taxa, arbuscular mycorrhizal fungi, ammonium. Overall, results suggest chemical fertilizer can be instrumental selecting beneficial communities associated phosphorus fitness.

Language: Английский

Citations

6
Soil legacy and organic amendment role in promoting the resistance of contaminated soils to drought DOI Creative Commons
Laura Morales, E. Fernández Boy, Engracia Madejón

et al.

Applied Soil Ecology, Journal Year: 2023, Volume and Issue: 195, P. 105226 - 105226

Published: Dec. 5, 2023

The application of organic amendments is a common practice in the restoration contaminated and degraded soils. We designed an experiment pot mesocosms to study effect amendment (comparing short-term addition biosolid compost with soils amended 17 years ago) soil legacy exposed or not extensive grazing over remediation process) on resistance Mediterranean against simulated drought event. Pots were sown forage mixture (Lolium rigidum Medicago polymorpha), was evaluated by measuring chemical properties, biological activity (soil enzyme activities respiration rate), microbial community composition plant production. Our results showed positive exposure increasing water retention stability production under conditions. long-term avoided negative consequences L. (the species lowest tolerance stress), while M. polymorpha biomass 12 times non-amended non-exposed soil. However, (enzyme rate) as well diversity limited conditions any type management legacy, demonstrating great adaptation communities stress semiarid Soils matter inputs long-term, through grazing, high similarities terms bacterial fungal composition.

Language: Английский

Citations

5
Quantitative reduction of soil bacteria and qualitative microbial changes: biotic components associated to kiwifruit decline DOI
L. Manici, Maria Ludovica Saccà, Carla Scotti

et al.

Plant and Soil, Journal Year: 2022, Volume and Issue: 477(1-2), P. 613 - 628

Published: May 2, 2022

Language: Английский

Citations

8
Tree Root-Associated Microbial Communities Depend on Various Floor Management Systems in an Intensive Apple (Malus × domestica Borkh.) Orchard DOI Open Access
Kamila Bokszczanin, Sebastian Przybyłko,

Karolina Molska-Kawulok

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(12), P. 9898 - 9898

Published: June 8, 2023

Regenerative 3agriculture prioritizes soil health to build up organic carbon and nitrogen stocks while supporting the active diverse biota that is a prerequisite for maintaining crop productivity quality in sustainable food production. This study aimed at unravelling impact of inorganic maintenance systems 'Red Jonaprince' apple (Malus × domestica Borkh.) orchard on microbiota biodiversity physico-chemical properties. During our study, we compared seven floor management terms microbial community diversity. Fungal bacterial communities all taxonomic levels differed largely between augmented matter (organic) other tested regimes. The dominant phylum was Ascomycota. operational units (OTUs) within Ascomycota were identified as members Sordariomycetes, followed by Agaricomycetes, both dominated versus inorganic. most prominent phyla, Proteobacteria, accounted 43% assigned bacteria OTUs. Gammaproteobacteria, Bacteroidia, Alphaproteobacteria predominant samples, Acidobacteriae, Verrucomicrobiae, Gemmatimonadetes more abundant mulches.

Language: Английский

Citations

4