Global abundance patterns, diversity, and ecology of Patescibacteria in wastewater treatment plants

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: March 16, 2024

Microorganisms are responsible for nutrient removal and resource recovery in wastewater treatment plants (WWTPs), their diversity is often studied by 16S rRNA gene amplicon sequencing. However, this approach underestimates the abundance of Patescibacteria due to low coverage commonly used PCR primers highly divergent bacterial phylum. Therefore, our current understanding global diversity, distribution, ecological role WWTPs very incomplete. This particularly relevant as considered be associated with microbial host cells can therefore influence temporal variability other groups that important WWTP functioning.

Language: Английский

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Role of Sedum alfredii and soil microbes in the remediation of ultra-high content heavy metals contaminated soil

Agriculture Ecosystems & Environment, Journal Year: 2022, Volume and Issue: 339, P. 108090 - 108090

Published: July 21, 2022

Language: Английский

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Genomic and metabolic adaptations of biofilms to ecological windows of opportunity in glacier-fed streams

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: April 20, 2022

In glacier-fed streams, ecological windows of opportunity allow complex microbial biofilms to develop and transiently form the basis food web, thereby controlling key ecosystem processes. Using metagenome-assembled genomes, we unravel strategies that seize this in an otherwise characterized by harsh environmental conditions. We observe a diverse microbiome spanning entire tree life including rich virome. Various co-existing energy acquisition pathways point niches exploitation available resources, likely fostering establishment during opportunity. The wide occurrence rhodopsins, besides chlorophyll, highlights role solar capture these while internal carbon nutrient cycling between photoautotrophs heterotrophs may help overcome constraints imposed oligotrophy habitats. Mechanisms potentially protecting bacteria against low temperatures high UV-radiation are also revealed selective pressure environment is further highlighted phylogenomic analysis differentiating important components stream from other ecosystems. Our findings reveal genomic underpinnings adaptive traits contributing success exploit opportunities which now rapidly changing owing global warming.

Language: Английский

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Genome-centric metagenomics reveals the host-driven dynamics and ecological role of CPR bacteria in an activated sludge system

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: March 22, 2023

Abstract Background Candidate phyla radiation (CPR) constitutes highly diverse bacteria with small cell sizes and are likely obligate intracellular symbionts. Given their distribution complex associations bacterial hosts, genetic biological features of CPR in low-nutrient environments have received increasing attention. However, wastewater treatment systems remain poorly understood. We utilized genome-centric metagenomics to answer how communities shift over 11 years what kind ecological roles they act an activated sludge system. Results found that approximately 9% (135) the 1,526 non-redundant archaeal metagenome-assembled genomes were affiliated CPR. consistently abundant a relative abundance up 7.5% studied The observed striking fluctuations community compositions limited metabolic biosynthetic capabilities collectively revealed nature dynamics may be directly determined by available hosts. Similarity-based network analysis further confirmed broad hosts lineages. proteome contents sludge-associated had higher similarity those environmental-associated than human-associated ones. Comparative genomic significant enrichment genes for oxygen stress resistance bacteria. Furthermore, carbon cycling horizontal gene transfer extensively identified genomes. Conclusions These findings highlight presence specific host interactions among lineages systems. Despite lack key pathways, these small, yet involvements biogeochemical evolution

Language: Английский

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Insight into dissolved organic nitrogen transformation and characteristics: Focus on printing and dyeing wastewater treatment process

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 450, P. 131086 - 131086

Published: Feb. 24, 2023

Language: Английский

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Metabolic Potential of the Superphylum <i>Patescibacteria</i> Reconstructed from Activated Sludge Samples from a Municipal Wastewater Treatment Plant

Microbes and Environments, Journal Year: 2022, Volume and Issue: 37(3), P. n/a - n/a

Published: Jan. 1, 2022

Patescibacteria are widely distributed in various environments and often detected activated sludge. However, limited information is currently available on their phylogeny, morphology, ecophysiological role sludge or interactions with other microorganisms. In the present study, we identified microorganisms that interacted via a correlation ana-lysis using 16S rRNA gene, predicted metabolic potential of metagenomic ana-lysis. The metagenome-assembled genomes consisted three Saccharimonadia, Parcubacteria, one Gracilibacteria, showed strong positive relative abundance Chitinophagales. Metabolic predictions from ten recovered patescibacterial five Chitinophagales supported mutualistic between member Saccharimonadia N-acetylglucosamine, Parcubacteria nitrogen compounds related to denitrification, Gracilibacteria phospholipids results indicate important for survival ecosystems.

Language: Английский

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Metabolic implications for predatory and parasitic bacterial lineages in activated sludge wastewater treatment systems

Water Research X, Journal Year: 2023, Volume and Issue: 20, P. 100196 - 100196

Published: Aug. 13, 2023

Deciphering unclear microbial interactions is key to improving biological wastewater treatment processes. Microbial predation and parasitism in ecosystems are unexplored survival strategies that have long been known recently attracted attention because these interspecies may contribute the reduction of excess sludge. Here, community profiling 600 activated sludge samples taken from six industrial one municipal processes (WWTPs) was conducted. To identify shared lineages WWTPs, constituents were defined as family level taxa had ≥ 0.1% average relative abundance detected all The analysis assigned 106 families WWTPs. Correlation showed 98 significantly correlated with total carbon (TC) and/or nitrogen (TN) concentrations, suggesting they remediation. Most possible predatory or parasitic bacteria belonging phyla Bdellovibrionota, Myxococcota, Candidatus Patescibacteria found be negatively TC/TN; thus, frequently present WWTPs could involved removal carbon/nitrogen derived cell components. Shotgun metagenome-resolved metabolic reconstructions indicated gene homologs associated conserved Ca. genomes (e.g., host interaction (hit) locus, Tad-like secretion complexes, type IV pilus assembly proteins). This study provides insights into complex potentially linked biomass

Language: Английский

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Autotrophic biofilms sustained by deeply sourced groundwater host diverse bacteria implicated in sulfur and hydrogen metabolism

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Jan. 26, 2024

Abstract Background Biofilms in sulfide-rich springs present intricate microbial communities that play pivotal roles biogeochemical cycling. We studied chemoautotrophically based biofilms host diverse CPR bacteria and grow to investigate controls on Results Sulfide were investigated using bulk geochemical analysis, genome-resolved metagenomics, scanning transmission X-ray microscopy (STXM) at room temperature 87 K. Chemolithotrophic sulfur-oxidizing bacteria, including Thiothrix Beggiatoa , dominate the biofilms, which also contain Gracilibacteria, Absconditabacteria, Saccharibacteria, Peregrinibacteria, Berkelbacteria, Microgenomates, Parcubacteria. STXM imaging revealed ultra-small cells near surfaces of filamentous may be bacterial episymbionts. NEXAFS spectroscopy carbon K sulfur L 2,3 edges show protein-encapsulated spherical elemental granules, indicating they are oxidizers, likely . Berkelbacteria Moranbacteria same biofilm sample predicted have a novel electron bifurcating group 3b [NiFe]-hydrogenase, putatively sulfhydrogenase, potentially linked metabolism via redox cofactors. This complex could contribute symbioses, for example, with such as is cryptic One Doudnabacteria genome encodes adjacent dioxygenase rhodanese genes convert thiosulfate sulfite. find similar conserved genomic architecture associated from other sulfur-rich subsurface ecosystems. Conclusions Our combined metagenomic, geochemical, spectromicroscopic, structural bioinformatics analyses growing consortia Proteobacteria, new family within Beggiatoales. infer hydrogen

Language: Английский

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Into the darkness of the microbial dark matter in situ activities through expression profiles of Patescibacteria populations

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 9, 2023

form a highly diverse and widespread superphylum of uncultured microorganisms representing third the global microbial diversity. Most our knowledge on

Language: Английский

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Groundwater biodiversity and constraints to biological distribution

Elsevier eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 113 - 140

Published: Jan. 1, 2023

Language: Английский

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