Fibrobacter sp. HC4, a newly isolated strain, demonstrates a high cellulolytic activity as revealed by enzymatic measurements and in vitro assay

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(8)

Published: July 31, 2024

Despite their low quantity and abundance, the cellulolytic bacteria that inhabit equine large intestine are vital to host, as they enable crucial use of forage-based diets.

Language: Английский

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Fibrolytic efficiency of the large intestine microbiota may benefit running speed in French trotters: A pilot study

Physiological Reports, Journal Year: 2024, Volume and Issue: 12(21)

Published: Nov. 1, 2024

Abstract This pilot study sought to explore the contribution of large intestine microbiota energy metabolism and exercise performance through its ability degrade fibers into short‐chain fatty acids (SCFAs). To investigate this, a correlational was carried out on athlete horses under same management conditions. Fecal diversity composition, fibrolytic efficiency SCFAs were analyzed. An incremental running test estimate maximal speed (MRS) horses, blood samples taken measure parameters. MRS positively correlated with fecal in degrading cellulose vitro ( r = 0.51; p 0.02). The abundance bacterial taxa not associated MRS, but functional inference analysis revealed positive association between pathways potentially related activity 0.54; 0.07 0.56; 0.05 for butyrate thiamine metabolism, respectively). In contrast, metabolic pathway starch degradation appeared negatively −0.55; 0.06). present findings suggest potential dietary digestion capacity equine athletes.

Language: Английский

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Microbial activity in herbivore dung affects nodulation and growth of Trifolium pratense

Plant and Soil, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Language: Английский

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Tunceli Yöresindeki At Dışkısı Örneklerinden İzole Edilen Anaerobik Gut Funguslarının (Piromyces spp.) Saf Kültürdeki Fibrolitik Aktiviteleri ve Moleküler Tanımlanması

International Journal of Pure and Applied Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 22, 2024

Bu çalışma, Türkiye'nin Tunceli ilindeki at dışkılarından anaerobik gut funguslarının (AGF) izolasyonu, karakterizasyonu ve enzim özelliklerine odaklanmaktadır. At dışkısından Piromyces cinsine ait iki izolat MUBAM_F1 MUBAM_F2, morfolojik moleküler teknikler kullanılarak izole edilmiş kısmen tanımlanmıştır. ITS bölgesinin Blast analizi, her izolatında cinsi içerisinde bir tür olduğu doğrulamıştır (sırasıyla %98.24 %98.87 benzerlik). Enzimatik analizler, için de önemli ksilanaz karboksimetil selülaz aktivitesinin olduğunu göstermiştir. MUBAM_F1, toplam 47.13±0.10 µmol/dak/ml (spesifik aktivite: 2153.93 µmol/dak/mg protein) aktivitesi gösterirken, 35.56 1626.52 57.53±0.08 µmol/dak/ml'de daha yüksek göstermiş 2630.11 ancak 23.93±1.41 düşük aktivitesine 1098.66 sahip belirlenmiştir. Çalışmada elde edilen veriler, sp. MUBAM_F2'nin kar-boksimetil enzimlerinin umut vadeden kaynakları göstermektedir.

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In vitro assessment of horse-isolated strains of Lactobacillus acidophilus and Ligilactobacillus equi species for fecal microbiota modulation in horses.

Journal of Equine Veterinary Science, Journal Year: 2024, Volume and Issue: 145, P. 105341 - 105341

Published: Dec. 31, 2024

Language: Английский

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Anaerobic fungi in the tortoise alimentary tract illuminate early stages of host-fungal symbiosis andNeocallimastigomycotaevolution

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 26, 2023

Abstract The anaerobic gut fungi (AGF, Neocallimastigomycota ) reside in the alimentary tract of herbivores. While their presence mammals is well documented, evidence for occurrence non-mammalian hosts currently sparse. Here we report on AGF communities tortoises (family Testudinidae ). Culture-independent surveys tortoise fecal samples identified a unique community, with three novel deep-branching genera representing >90% sequences most samples. Representatives all were successfully isolated under strict conditions at 30 ° C or 39 C. Transcriptomics-enabled phylogenomic and molecular dating analysis indicated an ancient, position tree these genera, evolutionary divergence time estimate 104-112 million years ago (Mya). Such estimates push establishment animal- symbiosis from early Paleogene (67 Mya) to Cretaceous (112 Mya). Further, compared mammalian counterparts, tortoise-associated isolates exhibited more limited capacity plant polysaccharides metabolism lacked genes encoding several carbohydrate active enzyme (CAZyme) families mediating degradation. Finally, demonstrate that observed curtailed degradation capacities reduced CAZyme repretoire driven by paucity horizontal gene transfer (HGT) genomes, massive HGT taxa. CAZyome overall secretory machinery also reflected altered cellulosomal production AGF. Our findings provide insights into scope phylogenetic diversity, ecological distribution, history, evolution fungal-host nutritional symbiosis, dynamics traits acquisition . Significance Anaerobic (AGF) are encountered rumen hindgut However, outside canonical unclear. We identification, isolation, characterization novel, tortoises. discovery expands diversity host range revises phylum’s tortoise-sourced lack multiple metabolic features identify relative events as major factor underpinning such differences. results alter our understanding history

Language: Английский

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Characterization of a novel multifunctional glycoside hydrolase family in the metagenome-assembled genomes of horse gut

Gene, Journal Year: 2024, Volume and Issue: 927, P. 148758 - 148758

Published: July 6, 2024

Language: Английский

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Rumen microbiota: Potential biocatalyst for biofuels

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 173 - 186

Published: Oct. 18, 2024

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Kinetic, genomic, and physiological analysis reveals diversity in the ecological adaptation and metabolic potential of Brachybacterium equifaecis sp. nov. isolated from horse feces

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(5)

Published: Sept. 14, 2023

Brachybacterium species have been identified in various ecological niches and belong to the family Dermabacteriaceae within phylum Actinobacteria. In this study, we isolated a novel equifaecis JHP9 strain from horse feces compared its kinetic, biochemical, genomic features with those of other strains. Moreover, comparative analysis using publicly available genomes was performed determine properties involved their adaptation metabolic potential. Novel delineation determined phylogenetically through 16S rRNA gene similarity (up 97.9%), average nucleotide identity (79.5-82.5%), amino acid (66.7-75.8%), silico DNA-DNA hybridization (23.7-27.9) closely related This study also presents first report kinetic species. Most strains displayed high oxygen (K m(app) =1.6-24.2 µM) glucose =0.73-1.22 affinities, which may manifest niche adaptations. Various carbohydrate metabolisms under aerobic anaerobic conditions, antibiotic resistance, mobile genetic elements, carbohydrate-active enzymes, lactic production, clustered regularly interspaced short palindromic repeats-Cas bacteriophage exclusion systems were observed genotypic and/or phenotypic species, suggesting genome flexibility, defense mechanisms, adaptability. Our contributes knowledge physiological, including strain, advocates for tolerant thriving nature environments, leading adaptation. IMPORTANCE Basic physiological most isolates studied; however, ability bacterium adapt diverse demonstrate role differentiation, is be yet. Therefore, here, explored cellular kinetics, diversity, adaptation/defensive analysis. addition, presented examining indicating that all Brachybacterium, one, affinity. Furthermore, revealed contains versatile properties, provide significant competitive advantages. Thus, in-depth at level genus beneficial clarifying differential characteristics, conferring inhabit niches.

Language: Английский

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Anaerobic fungi in the tortoise alimentary tract illuminate early stages of host-fungal symbiosis and Neocallimastigomycota evolution

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 14, 2023

Abstract The anaerobic gut fungi (AGF, Neocallimastigomycota ) reside in the alimentary tract of herbivores. While their presence mammals is well documented, evidence for occurrence non-mammalian hosts currently sparse. Here we report on AGF communities tortoises (family Testudinidae ). Culture-independent surveys tortoise fecal samples identified a unique community, with three novel deep-branching genera representing > 90% sequences most samples. Representatives all were successfully isolated under strict conditions at 30 º C or 39 C. Transcriptomics-enabled phylogenomic and molecular dating analysis indicated an ancient, position tree these genera, evolutionary divergence time estimate 104–112 million years ago (Mya). Such estimates push establishment animal- symbiosis from early Paleogene (67 Mya) to Cretaceous (112 Mya). Further, compared mammalian counterparts, tortoise-associated isolates exhibited more limited capacity plant polysaccharides metabolism lacked genes encoding several carbohydrate active enzyme (CAZyme) families mediating degradation. Finally, demonstrate that observed curtailed degradation capacities reduced CAZyme repretoire driven by paucity horizontal gene transfer (HGT) genomes, massive HGT taxa. CAZyome overall secretory machinery also reflected altered cellulosomal production AGF. Our findings provide insights into scope phylogenetic diversity, ecological distribution, history, evolution fungal-host nutritional symbiosis, dynamics traits acquisition .

Language: Английский

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