Revealing the anti-senescence effects and related mechanisms of flavonoid extracts from the buds of Wikstroemia chamaedaphne Meisn on D-galactose-induced PC12 cells based on network pharmacology and transcriptomics DOI Creative Commons
Xiu-Ying Zhao,

Wen-Qian Liu,

Liwei Zhang

et al.

Future Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 10, 2025

Abstract Background Natural products are an important source of drugs or lead compounds for the treatment senescence. The buds Wikstroemia chamaedaphne Meisn a traditional Chinese medicine to cure edema, schizophrenia and epilepsy. A flavonoid extract W. (FEW) was prepared from methanolic by our group previously, which including eight flavonoids with content (55.19 ± 0.32) %. In this study, anti-senescence effects related mechanisms FEW on D-galactose-induced PC12 cells were investigated first time. Results High doses D-galactose could induce cell senescence, whereas delay senescence decreasing SA-β-gal positivity, increasing SOD activity, reducing MDA levels, improving morphology, inhibiting cycle arrest down-regulating expression senescence-related proteins P16, P21 P53. Subsequently, potential underlying elucidated through integration network pharmacology transcriptomics. main signaling pathways involved found be cancer pathway, FOXO PI3k–Akt AGE–RAGE protein digestion uptake, etc. may pathway as revealed western blot experiments. Conclusion Our study that has effects. This suggest acts agent age-related neurological diseases.

Language: Английский

Revealing the anti-senescence effects and related mechanisms of flavonoid extracts from the buds of Wikstroemia chamaedaphne Meisn on D-galactose-induced PC12 cells based on network pharmacology and transcriptomics DOI Creative Commons
Xiu-Ying Zhao,

Wen-Qian Liu,

Liwei Zhang

et al.

Future Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 10, 2025

Abstract Background Natural products are an important source of drugs or lead compounds for the treatment senescence. The buds Wikstroemia chamaedaphne Meisn a traditional Chinese medicine to cure edema, schizophrenia and epilepsy. A flavonoid extract W. (FEW) was prepared from methanolic by our group previously, which including eight flavonoids with content (55.19 ± 0.32) %. In this study, anti-senescence effects related mechanisms FEW on D-galactose-induced PC12 cells were investigated first time. Results High doses D-galactose could induce cell senescence, whereas delay senescence decreasing SA-β-gal positivity, increasing SOD activity, reducing MDA levels, improving morphology, inhibiting cycle arrest down-regulating expression senescence-related proteins P16, P21 P53. Subsequently, potential underlying elucidated through integration network pharmacology transcriptomics. main signaling pathways involved found be cancer pathway, FOXO PI3k–Akt AGE–RAGE protein digestion uptake, etc. may pathway as revealed western blot experiments. Conclusion Our study that has effects. This suggest acts agent age-related neurological diseases.

Language: Английский

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