Production of metabolites from mutant strains of Rhodotorula minuta upon varied temperature DOI Creative Commons

G A Gowthami,

B. S. Gunashree

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 29, 2023

Abstract The present investigation focussed on the production of metabolites such as phytase, carotenoid pigment, lipids, and exopolysaccharide content by a hyper producing putative mutant strain Rhodotorula minuta . Several mutants namely MR5, MR7, MR9, MR13, CMR1, CMR2 were isolated after series UV chemical (Ethyl methane sulfonate, EMS) mutagenesis an orange yeast strain, effect different temperatures these was tested. result showed highest phytase from CMR1 (26.735 U/ml) MR7 (21.620 at 30 35°C respectively. With respect to production, yield 1000 1300 mg/l obtained 40°C with MR9 CMR2, respectively, while lipid MR5 (34 g/kg) (11 25°C. Total in MR13 445 µg/kg it found be 454 dry cell weight All strains produced amount one or other tested metabolites. both good maximum accumulation cells.

Language: Английский

Microbial Lipid Based Biorefinery Concepts: A Review of Status and Prospects DOI Creative Commons
Jonilson de Melo e Silva, Luiza Helena da Silva Martins, Débora Kono Taketa Moreira

et al.

Foods, Journal Year: 2023, Volume and Issue: 12(10), P. 2074 - 2074

Published: May 22, 2023

The use of lignocellulosic biomass as a raw material for the production lipids has gained increasing attention, especially in recent years when food biofuels become current technology. Thus, competition materials both uses brought need to create technological alternatives reduce this that could generate reduction volume offered and consequent commercial increase value food. Furthermore, microbial oils been studied many industrial branches, from generation renewable energy obtainment several value-added products pharmaceutical industries. review provides an overview feasibility challenges observed through biorefinery. Topics covered include biorefining technology, oil market, oily microorganisms, mechanisms involved lipid-producing metabolism, strain development, processes, lipids, technical drawbacks, lipid recovery.

Language: Английский

Citations

27
Sustainable lipid production by oleaginous yeasts: Current outlook and challenges DOI
Kukkala Kiran Kumar, Farha Deeba, Ajay Kumar Pandey

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132205 - 132205

Published: Feb. 1, 2025

Language: Английский

Citations

1
Oleaginous yeasts: Biodiversity and cultivation DOI Open Access

Rujiralai Poontawee,

Wanlapa Lorliam, Pirapan Polburee

et al.

Fungal Biology Reviews, Journal Year: 2022, Volume and Issue: 44, P. 100295 - 100295

Published: Dec. 5, 2022

Language: Английский

Citations

31
Exploring Yeast Diversity to Produce Lipid-Based Biofuels from Agro-Forestry and Industrial Organic Residues DOI Creative Commons
Marta Mota,

Paula Múgica,

Isabel Sá‐Correia

et al.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(7), P. 687 - 687

Published: June 29, 2022

Exploration of yeast diversity for the sustainable production biofuels, in particular biodiesel, is gaining momentum recent years. However, sustainable, and economically viable bioprocesses require strains exhibiting: (i) high tolerance to multiple bioprocess-related stresses, including various chemical inhibitors present hydrolysates from lignocellulosic biomass residues; (ii) ability efficiently consume all major carbon sources present; (iii) capacity produce lipids with adequate composition yields. More than 160 non-conventional (non-

Language: Английский

Citations

30
Biotechnological potential of red yeast isolated from birch forests in Poland DOI Creative Commons
Anna M. Kot,

Paulina Laszek,

Marek Kieliszek

et al.

Biotechnology Letters, Journal Year: 2024, Volume and Issue: 46(4), P. 641 - 669

Published: April 30, 2024

Abstract Objectives This study aimed to isolate red yeast from sap, bark and slime exudates collected Polish birch forests then assessment of their biotechnological potential. Results 24 strains were isolated the bark, sap spring fluxes ( Betula pendula ). Strains belonging Rhodotorula mucilaginosa (6), Rhodosporidiobolus colostri (4), Cystrofilobasidium capitaum (3), Phaffia rhodozyma (3) Cystobasidium psychroaquaticum dominant. The highest efficiency carotenoid biosynthesis (5.04 mg L −1 ) was obtained by R. CMIFS 004, while lipids most efficiently produced two P. (5.40 5.33 g amount exopolysaccharides (3.75 glutinis 103. Eleven showed lipolytic activity, nine amylolytic only proteolytic activity. presence biosurfactants not found. growth species pathogenic moulds best inhibited yeasts. Conclusion Silver is a good natural source for isolation new with wide

Language: Английский

Citations

6
A concise review of technologies for converting forest biomass to bioenergy DOI Creative Commons
Asif Raihan

Journal of Technology Innovations and Energy, Journal Year: 2023, Volume and Issue: 2(3), P. 10 - 36

Published: Aug. 18, 2023

The use of biomass is vital in reducing the negative effects rising fossil fuel consumption. Given its quantity and diversity, forest has garnered a lot interest among many kinds biomass. This study evaluates various strategies for transforming woody waste into usable biofuels. Carbon dioxide emissions from traditional energy generation systems could be mitigated through direct utilization Low conversion rates, as well soot residues, are some problems that come up when directly using sustainability also seriously threatened by lack constant access to Co-combustion with coal pelletizing two solutions proposed this issue. potential lower process's carbon monoxide, nitrogen oxides, sulfides. article reviews discusses biochemical thermochemical mechanisms can transform variety liquid gaseous Future research cutting-edge assessment tools like life cycle assessment, exergy, etc. should investigate processes bioenergy further.

Language: Английский

Citations

13
Diversity of Red Yeasts in Various Regions and Environments of Poland and Biotechnological Potential of the Isolated Strains DOI Creative Commons
Anna M. Kot,

Wioletta Sęk,

Marek Kieliszek

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2023, Volume and Issue: 196(6), P. 3274 - 3316

Published: Aug. 30, 2023

Due to the growing demand for natural carotenoids, researchers have been searching strains that are capable of efficient synthesis these compounds. This study tested 114 red yeast collected from various environments and food specimens in Poland. The were isolated by their ability produce or yellow pigments rich nutrient media. According potential industrial significance both total production share individual carotenoids (β-carotene, γ-carotene, torulene, torularhodin) analyzed. content carotenoid dry matter ranged 13.88 406.50 µg/g, percentages highly varied among strains. Most isolates synthesized torulene at highest amount. Among studied strains, with a biomass greater than 200 µg/g those containing more 60% torularhodin selected identification (48 strains). identified belonged six genera: Rhodotorula, Sporidiobolus, Sporobolomyces, Buckleyzyma, Cystofilobasidium, Erythrobasidium. largest number Rhodotorula babjevae (18), mucilaginosa (7), Sporidiobolus pararoseus (4), glutinis (4).

Language: Английский

Citations

13
Production of sustainable aviation fuel precursors using the oleaginous yeast Rhodotorula toruloides PYCC 5615 cultivated on eucalyptus bark hydrolysate DOI
Teresa Lopes da Silva,

Francisca Dutra,

Susana Marques

et al.

Biomass and Bioenergy, Journal Year: 2025, Volume and Issue: 197, P. 107790 - 107790

Published: March 18, 2025

Language: Английский

Citations

0
Unveiling the anticancer potentiality of single cell oils produced by marine oleaginous Paradendryphiella sp. under optimized economic growth conditions DOI Creative Commons

Hadeel El‐Shall,

Marwa M. Abu‐Serie, Gadallah Abu‐Elreesh

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 26, 2023

Abstract Bioprospecting about new marine oleaginous fungi that produce advantageous bioproducts in a green sustainable process is the key of blue bioeconomy. Herein, Paradendryphiella sp. was utilized for single cell oils (SCOs) production economically, via central composite design, lipid content enhanced 2.2-fold by 5.5 g/L yeild on seawater-based media supplemented with molasses concentration 50 g/L, yeast extract, 2.25 at initial pH value (5.3) and 8 days static incubation. Subsequently, fatty acid methyl esters profiles SCOs produced optimized under different abiotic conditions were determined; signifying qualitative quantitative variations. Interestingly, psychrophilic-prolonged incubation increased unsaturation level acids to 59.34%, while ω-6 ω-3 contents representing 23.53% 0.67% respectively. Remarkably, it exhibited highest EC 100 dose 677.03 µg/mL normal human lung fibroblast Wi-38 cells. Meanwhile, showed inhibiting proliferation potential cancer lines A549, MDA-MB 231 HepG-2 cells 372.37, 417.48 365.00 µg/mL, Besides, elevated oxidative stress, expression apoptotic genes suppressed oncogenes (NF-κB, BCL2 cyclin D); implying its promising efficacy treatment as adjuvant drug. This study denoted lipogenesis capacity acidic/alkaline psychrophilic/mesophilic conditions. Hereby attaining efficient economic seasonal variation Egyptian sources fill gap freshwater crisis simultaneously preserve energy.

Language: Английский

Citations

9
Diversity of duckweed (<i>Lemnaceae</i>) associated yeasts and their plant growth promoting characteristics DOI Creative Commons

Napapohn Kajadpai,

Jirameth Angchuan,

Pannida Khunnamwong

et al.

AIMS Microbiology, Journal Year: 2023, Volume and Issue: 9(3), P. 486 - 517

Published: Jan. 1, 2023

The diversity of duckweed (Lemnaceae) associated yeasts was studied using a culture-dependent method. A total 252 yeast strains were isolated from 53 samples out the 72 collected 16 provinces in Thailand. Yeast identification conducted based on D1/D2 region large subunit (LSU) rRNA gene sequence analysis. It revealed that 55.2% and 44.8% species Ascomycota Basidiomycota yeasts, respectively. Among all, Papiliotrema laurentii, basidiomycetous yeast, found as most prevalent showing relative frequency occurrence 21.8% 25%, In this study, high index values shown, indicated by Shannon-Wiener (H'), Shannon equitability (EH) Simpson (1-D) 3.48, 0.86 0.96, present results community had increased diversity, with evenness among species. Principal coordinate analysis (PCoA) no marked differences communities genera. accumulation curve showed observed richness lower than expected. Investigation plant growth promoting traits 178 produced indole-3-acetic acid (IAA) at levels ranging 0.08-688.93 mg/L. Moreover, siderophore production phosphate solubilization also studied. One hundred seventy-three siderophores exhibited 0.94-2.55 activity units (AU). six activity, expressed efficiency (SE) units, range 0.32-2.13 SE. This work indicates is potential microbial resource can be used for promotion.

Language: Английский

Citations

8