Using
sequential
excitation
with
a
minimum
of
light
to
localize
single
fluorescent
molecules
represented
breakthrough
because
it
delivers
1-2
nm
precision
moderate
photon
counts,
enabling
tracking
and
super-resolution
imaging
true
molecular
resolution.
Expanding
this
concept
multi-photon
regimes
may
be
useful
complement
reach
even
higher
localization
get
deeper
into
biological
specimens.
Advanced Optical Materials,
Journal Year:
2022,
Volume and Issue:
10(24)
Published: Oct. 26, 2022
Abstract
Near‐infrared
phosphor‐converted
light‐emitting
diodes
(NIR
pc‐LEDs)
have
promising
applications
in
food
analysis,
night
vision
imaging,
and
biological
probes.
Developing
NIR
phosphors
with
broadband
emission
high
efficiency
has
attracted
immense
interest.
Herein,
a
novel
phosphor
Ca
2
LuScAl
Si
O
12
:Cr
3+
(CLSAS:Cr
)
is
reported
for
the
first
time.
Under
excitation
of
442
nm
light,
CLSAS:Cr
shows
from
600
to
900
full
width
at
half
maximum
(FWHM)
142
nm,
benefiting
emissions
two
Cr
centers
CLSAS,
which
proved
by
spectra
decay
curves.
The
internal
quantum
(IQE)
73.7%
achieved
and,
423
K,
intensity
maintains
76%
value
room
temperature.
Combining
450
blue
chip,
pc‐LED
fabricated
demonstrated
fast
imaging
veins
human
palm
fist,
as
well
objects.
Advanced Optical Materials,
Journal Year:
2023,
Volume and Issue:
11(21)
Published: June 7, 2023
Abstract
Cr
3+
‐activated
Gd
3
Ga
5
O
12
garnet
(GGG:Cr
)
near‐infrared
(NIR)
phosphors
have
shown
promising
applications
in
regulating
plant
growth.
However,
the
low
external
quantum
efficiency
(EQE)
results
a
wall‐plug
(WPE)
of
phosphor‐converted
light‐emitting
diodes
(pc)‐LEDs
only
≈15%.
Herein,
an
easy
strategy
to
improve
luminescent
properties
GGG:Cr
phosphor
is
reported.
Through
optimization
synthesized
technology,
EQE
enhanced
43.6%.
Remarkably,
fabricated
pc‐LEDs
achieve
WPE
as
high
34.3%.
These
demonstrate
significant
advancement
development
and
NIR
materials
pc‐LED
devices.
eLight,
Journal Year:
2022,
Volume and Issue:
2(1)
Published: March 25, 2022
Abstract
Achieving
localization
with
molecular
precision
has
been
of
great
interest
for
extending
fluorescence
microscopy
to
nanoscopy.
MINFLUX
pioneers
this
transition
through
point
spread
function
(PSF)
engineering,
yet
its
performance
is
primarily
limited
by
the
signal-to-background
ratio.
Here
we
demonstrate
theoretically
that
two-photon
(2p-MINFLUX)
could
double
PSF
engineering
nonlinear
effect.
Cramér-Rao
Bound
(CRB)
studied
as
maximum
precision,
and
CRB
halved
compared
single-photon
(1p-MINFLUX)
in
all
three
dimensions.
Meanwhile,
order
achieve
same
1p-MINFLUX,
2p-MINFLUX
requires
only
1/4
photons.
Exploiting
simultaneous
excitation
multiple
fluorophore
species,
may
have
potential
registration-free
nanoscopy
multicolor
tracking.
Localization
of
single
fluorescent
emitters
is
key
for
physicochemical
and
biophysical
measurements
at
the
nanoscale
beyond
ensemble
averaging.
Examples
include
single-molecule
tracking
super-resolution
imaging
by
localization
microscopy.
Among
numerous
methods
available,
MINFLUX
outstands
achieving
a
~10-fold
improvement
in
resolution
over
wide-field
camera-based
approaches,
reaching
molecular
scale
moderate
photon
counts.
Widespread
application
related
has
been
hindered
technical
complexity
setups.
Here,
we
present
RASTMIN,
method
based
on
raster
scanning
light
pattern
comprising
minimum
intensity.
RASTMIN
delivers
~1-2
nm
precision
with
usual
fluorophores
easily
implementable
standard
confocal
microscope
few
modifications.
We
demonstrate
performance
molecules
DNA
origami
structures.
In
fluorescence
microscopy,
computational
algorithms
have
been
developed
to
suppress
noise,
enhance
contrast,
and
even
enable
super-resolution
(SR).
However,
the
local
quality
of
images
may
vary
on
multiple
scales,
these
differences
can
lead
misconceptions.
Current
mapping
methods
fail
finely
estimate
quality,
challenging
associate
SR
scale
content.
Here,
we
develop
a
rolling
Fourier
ring
correlation
(rFRC)
method
evaluate
reconstruction
uncertainties
down
scale.
To
visually
pinpoint
regions
with
low
reliability,
filtered
rFRC
is
combined
modified
resolution-scaled
error
map
(RSM),
offering
comprehensive
concise
for
further
examination.
We
demonstrate
their
performances
various
imaging
modalities,
resulting
quantitative
maps
better
integrated
from
different
reconstructions.
Overall,
expect
that
our
framework
become
routinely
used
tool
biologists
in
assessing
image
datasets
general
inspire
advances
rapidly
developing
field
imaging.
Abstract
Mitochondria
are
crucial
organelles
closely
associated
with
cellular
metabolism
and
function.
Mitochondrial
DNA
(mtDNA)
encodes
a
variety
of
transcripts
proteins
essential
for
However,
the
interaction
between
inner
membrane
(IM)
mtDNA
remains
elusive
due
to
limitations
in
spatiotemporal
resolution
offered
by
conventional
microscopy
absence
suitable
vivo
probes
specifically
targeting
IM.
Here,
we
have
developed
novel
fluorescence
probe
called
HBmito
Crimson,
characterized
exceptional
photostability,
fluorogenicity
within
lipid
membranes,
low
saturation
power.
We
successfully
achieved
over
500
frames
low-power
stimulated
emission
depletion
(STED)
imaging
visualize
IM
dynamics,
spatial
40
nm.
By
utilizing
dual-color
mtDNA,
it
has
been
uncovered
that
tends
habitat
at
mitochondrial
tips
or
branch
points,
exhibiting
an
overall
spatially
uniform
distribution.
Notably,
dynamics
mitochondria
intricately
positioning
fusion
consistently
occurs
close
proximity
minimize
pressure
during
cristae
remodeling.
In
healthy
cells,
>66%
Class
III
(i.e.,
>5
μm
>12
cristae),
while
dropped
<18%
ferroptosis.
orchestrated
remodeling,
foster
even
distribution
mtDNA.
Conversely,
conditions
apoptosis
ferroptosis
where
structure
is
compromised,
becomes
irregular.
These
findings,
unprecedented
resolution,
reveal
intricate
interplay
provide
insights
into
driving
forces
behind
Abstract
We
report
the
development
of
a
head-mounted
photoacoustic
fiberscope
for
cerebral
imaging
in
freely
behaving
mouse.
The
4.5-gram
probe
has
9-µm
lateral
resolution
and
0.2-Hz
frame
rate
over
1.2-mm
wide
area.
can
continuously
monitor
oxygenation
hemodynamic
responses
at
single-vessel
resolution,
showing
significantly
different
cerebrovascular
to
external
stimuli
under
anesthesia
moving
state.
For
example,
when
subjected
high-concentration
CO
2
respiration,
enhanced
compensate
hypercapnia
be
visualized
due
regulation
Comparative
studies
exhibit
weakened
compensation
capabilities
obese
rodents.
This
new
modality
used
investigating
both
normal
pathological
functions
shows
great
promise
studying
activity,
disorders
their
treatments.
Abstract
Near‐infrared
(NIR)
phosphor‐converted
(pc)
light‐emitting
diodes
(LEDs)
are
gaining
popularity
as
smart
NIR
spectroscopy
light
sources.
However,
the
low
wall‐plug
efficiency
(WPE)
of
pc‐LEDs
has
limited
their
applications
due
to
comprehensive
properties
external
quantum
efficiencies
(EQEs)
and
thermal
stability
for
phosphors.
In
this
study,
a
simple
strategy
is
presented
achieve
high‐performance
phosphors
by
breaking
d–d
parity
forbidden
transitions
Cr
3+
in
garnet
host
introducing
asymmetric
vibrations
via
lattice
distortions.
The
achieved
EQE
high
44%
at
room
temperature
maintains
>98%
initial
150
°C,
demonstrating
excellent
stability.
Notably,
these
enable
unprecedented
with
record
WPE
26%
driven
100
mA.
Consequently,
application
expanded
new
areas
such
real‐time
heart
rate
monitoring
fingerprint
recognition.
