Developmental Cell, Journal Year: 2023, Volume and Issue: 58(10), P. 885 - 897.e4
Published: April 10, 2023
Language: Английский
Nature Reviews Endocrinology, Journal Year: 2020, Volume and Issue: 16(9), P. 479 - 494
Published: June 29, 2020
Language: Английский
Citations
274
Proceedings of the National Academy of Sciences, Journal Year: 2019, Volume and Issue: 116(46), P. 23132 - 23142
Published: Oct. 30, 2019
The human endometrium is essential in providing the site for implantation and maintaining growth survival of conceptus. An unreceptive disrupted maternal−conceptus interactions can cause infertility due to pregnancy loss or later complications. Despite this, role uterine glands first trimester little understood. established organoid protocol was used generate comprehensively analyze 3-dimensional endometrial epithelial (EEO) cultures from biopsies. derived EEO expand long-term, are genetically stable, be cryopreserved. Using 2 different donors, were then treated with estrogen (E2) d E2 medroxyprogesterone acetate (MPA) 6 d. cells positive gland marker, FOXA2, exhibited appropriate hormonal regulation steroid hormone receptor expression. Real-time qPCR bulk RNA-sequencing analysis revealed effects treatment on gene expression that recapitulated changes proliferative secretory phase endometrium. Single-cell RNA sequencing several cell types present whose proportion changed treatment. model serves as an important platform studying physiology pathology
Language: Английский
Citations
185
Trends in Endocrinology and Metabolism, Journal Year: 2019, Volume and Issue: 31(2), P. 165 - 180
Published: Dec. 20, 2019
Language: Английский
Citations
90
Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)
Published: June 7, 2021
Abstract During early pregnancy in the mouse, nidatory estrogen (E2) stimulates endometrial receptivity by activating a network of signaling pathways that is not yet fully characterized. Here, we report bone morphogenetic proteins (BMPs) control via conserved activin receptor type 2 A (ACVR2A) and SMAD1/5 pathway. Mice were generated to contain single or double conditional deletion ACVR2A/ACVR2B receptors using progesterone (PR)-cre. Female mice with display defects result development cystic glands, hyperproliferative epithelium during window implantation, impaired apicobasal transformation prevents embryo implantation leads infertility. Analysis Acvr2a- PRcre Acvr2b -PRcre pregnant determined BMP occurs ACVR2A ACVR2B dispensable implantation. Therefore, BMPs signal through ACVR2A/SMAD1/5 pathway promotes
Language: Английский
Citations
69
Nature Reviews Endocrinology, Journal Year: 2022, Volume and Issue: 18(12), P. 727 - 743
Published: Sept. 1, 2022
Language: Английский
Citations
49
Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(44)
Published: Oct. 24, 2022
Organoid technology has provided unique insights into human organ development, function, and diseases. Patient-derived organoids are increasingly used for drug screening, modeling rare disorders, designing regenerative therapies, understanding disease pathogenesis. However, the use of Matrigel to grow represents a major challenge in clinical translation organoid technology. is poorly defined mixture extracellular matrix proteins growth factors extracted from Engelbreth–Holm–Swarm mouse tumor. The driver multiple cellular processes differs significantly between tissues as well healthy states same tissue. Therefore, we envisioned that derived native tissue would be able support akin organogenesis vivo. Here, have developed hydrogels decellularized bovine endometrium. These supported endometrial organoids, which was comparable Matrigel. Organoids grown were proteomically more similar than those cultured Proteomic Raman microspectroscopy analyses showed method decellularization affects biochemical composition and, subsequently, their ability growth. amount laminin correlated with number shape organoids. We also demonstrated utility developing solid scaffolds supporting high-throughput, cell culture–based applications. In summary, overcome limitation greatly expand applicability understand biology associated pathologies.
Language: Английский
Citations
46
Proceedings of the Royal Society B Biological Sciences, Journal Year: 2023, Volume and Issue: 290(1997)
Published: April 18, 2023
The placenta has evolved to support the development of embryo and fetus during different intrauterine periods life. By necessity, its must precede that embryo. There is now evidence embryogenesis organogenesis, human supported by histotrophic nutrition secreted from endometrial glands rather than maternal blood. These secretions provide a plentiful supply glucose, lipids, glycoproteins growth factors stimulate rapid proliferation differentiation villous trophoblast. Furthermore, gland organoids indicates expression secretion these products are upregulated following sequential exposure oestrogen, progesterone trophoblastic decidual hormones, in particular prolactin. Hence, feed-forward signalling dialogue proposed among trophoblast, decidua enables own development, independent Many common complications pregnancy represent spectrum disorders associated with deficient trophoblast proliferation. Increasing suggests this mirrored one impaired decidualization, potentially compromising histotroph through diminished prolactin reduced function. Optimizing wellbeing prior conception may therefore help prevent complications, such as miscarriage, restriction pre-eclampsia.
Language: Английский
Citations
42
Med, Journal Year: 2023, Volume and Issue: 4(8), P. 554 - 579.e9
Published: Aug. 1, 2023
Language: Английский
Citations
41
Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 13
Published: Jan. 17, 2023
It is estimated that more and couples suffer from fertility pregnancy maintenance disorders. associated with impaired androgen secretion, which influenced by many factors, ranging genetic to environmental. also important remember disorders can result abnormal anatomy of the reproductive male female organ (congenital uterine anomalies – septate, unicornuate, bicornuate uterus; acquired defects uterus structure fibroids, polyps, hypertrophy), disturbed hormonal cycle obstruction fallopian tubes resulting presence adhesions due inflammation, endometriosis, surgery, rhythm menstrual bleeding, concentration hormones. There are relationships between endocrine organs, leading a chain reaction when one them fails function properly. Conditions in immune system involved, including infections autoimmune diseases, affect fertility. The form treatment depends on infertility duration patient’s age. includes ovulation stimulation clomiphene citrate or gonadotropins, metformin use, weight loss interventions. Since so different factors fertility, it correctly diagnose what causing problem modify regimen if necessary. This review describes disturbances hormone secretion individual organs context pregnancy.
Language: Английский
Citations
32
Annual Review of Immunology, Journal Year: 2023, Volume and Issue: 41(1), P. 127 - 151
Published: Jan. 11, 2023
The presence of granulated lymphocytes in the human uterine mucosa, known as decidua during pregnancy, or endometrium otherwise, was first noted nineteenth century, but it not until 1990 that these cells were identified a type natural killer (NK) cell. From outset, NK (uNK) found to be less cytotoxic than their circulating counterparts, peripheral (pNK) cells. Recently, unbiased approaches have defined three subpopulations uNK cells, all which cluster separately from pNK Here, we review history research into including ability interact with placental extravillous trophoblast and potential role regulating implantation. We go on more recent advances focus cell development heterogeneity defend against infection mediate memory effects. Finally, consider how better understanding could leveraged future improve outcomes pregnancy for mothers babies.
Language: Английский
Citations
26