Beclin-1-dependent autophagy, but not apoptosis, is critical for stem-cell-mediated endometrial programming and the establishment of pregnancy DOI Creative Commons

Pooja Popli,

Suni Tang,

Sangappa B. Chadchan

et al.

Developmental Cell, Journal Year: 2023, Volume and Issue: 58(10), P. 885 - 897.e4

Published: April 10, 2023

Language: Английский

Tracking placental development in health and disease DOI
John Aplin, Jenny Myers, Kate Timms

et al.

Nature Reviews Endocrinology, Journal Year: 2020, Volume and Issue: 16(9), P. 479 - 494

Published: June 29, 2020

Language: Английский

Citations

274

Self-renewing endometrial epithelial organoids of the human uterus DOI Open Access
Harriet C. Fitzgerald, Pramod Dhakal, Susanta K. Behura

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2019, Volume and Issue: 116(46), P. 23132 - 23142

Published: Oct. 30, 2019

The human endometrium is essential in providing the site for implantation and maintaining growth survival of conceptus. An unreceptive disrupted maternal−conceptus interactions can cause infertility due to pregnancy loss or later complications. Despite this, role uterine glands first trimester little understood. established organoid protocol was used generate comprehensively analyze 3-dimensional endometrial epithelial (EEO) cultures from biopsies. derived EEO expand long-term, are genetically stable, be cryopreserved. Using 2 different donors, were then treated with estrogen (E2) d E2 medroxyprogesterone acetate (MPA) 6 d. cells positive gland marker, FOXA2, exhibited appropriate hormonal regulation steroid hormone receptor expression. Real-time qPCR bulk RNA-sequencing analysis revealed effects treatment on gene expression that recapitulated changes proliferative secretory phase endometrium. Single-cell RNA sequencing several cell types present whose proportion changed treatment. model serves as an important platform studying physiology pathology

Language: Английский

Citations

185

Uterine Luminal Epithelium as the Transient Gateway for Embryo Implantation DOI

Xiaoqin Ye

Trends in Endocrinology and Metabolism, Journal Year: 2019, Volume and Issue: 31(2), P. 165 - 180

Published: Dec. 20, 2019

Language: Английский

Citations

90

Endometrial receptivity and implantation require uterine BMP signaling through an ACVR2A-SMAD1/SMAD5 axis DOI Creative Commons
Diana Monsivais, Takashi Nagashima, Renata Prunskaitė-Hyyryläinen

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: June 7, 2021

Abstract During early pregnancy in the mouse, nidatory estrogen (E2) stimulates endometrial receptivity by activating a network of signaling pathways that is not yet fully characterized. Here, we report bone morphogenetic proteins (BMPs) control via conserved activin receptor type 2 A (ACVR2A) and SMAD1/5 pathway. Mice were generated to contain single or double conditional deletion ACVR2A/ACVR2B receptors using progesterone (PR)-cre. Female mice with display defects result development cystic glands, hyperproliferative epithelium during window implantation, impaired apicobasal transformation prevents embryo implantation leads infertility. Analysis Acvr2a- PRcre Acvr2b -PRcre pregnant determined BMP occurs ACVR2A ACVR2B dispensable implantation. Therefore, BMPs signal through ACVR2A/SMAD1/5 pathway promotes

Language: Английский

Citations

69

Strategies for modelling endometrial diseases DOI
Alina R. Murphy, Hannes Campo, Julie Kim

et al.

Nature Reviews Endocrinology, Journal Year: 2022, Volume and Issue: 18(12), P. 727 - 743

Published: Sept. 1, 2022

Language: Английский

Citations

49

Bovine and human endometrium-derived hydrogels support organoid culture from healthy and cancerous tissues DOI Creative Commons
M. Fairuz B. Jamaluddin, Arnab Ghosh,

Aviraj Ingle

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(44)

Published: Oct. 24, 2022

Organoid technology has provided unique insights into human organ development, function, and diseases. Patient-derived organoids are increasingly used for drug screening, modeling rare disorders, designing regenerative therapies, understanding disease pathogenesis. However, the use of Matrigel to grow represents a major challenge in clinical translation organoid technology. is poorly defined mixture extracellular matrix proteins growth factors extracted from Engelbreth–Holm–Swarm mouse tumor. The driver multiple cellular processes differs significantly between tissues as well healthy states same tissue. Therefore, we envisioned that derived native tissue would be able support akin organogenesis vivo. Here, have developed hydrogels decellularized bovine endometrium. These supported endometrial organoids, which was comparable Matrigel. Organoids grown were proteomically more similar than those cultured Proteomic Raman microspectroscopy analyses showed method decellularization affects biochemical composition and, subsequently, their ability growth. amount laminin correlated with number shape organoids. We also demonstrated utility developing solid scaffolds supporting high-throughput, cell culture–based applications. In summary, overcome limitation greatly expand applicability understand biology associated pathologies.

Language: Английский

Citations

46

The human placenta: new perspectives on its formation and function during early pregnancy DOI Creative Commons

Graham J. Burton,

Eric Jauniaux

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2023, Volume and Issue: 290(1997)

Published: April 18, 2023

The placenta has evolved to support the development of embryo and fetus during different intrauterine periods life. By necessity, its must precede that embryo. There is now evidence embryogenesis organogenesis, human supported by histotrophic nutrition secreted from endometrial glands rather than maternal blood. These secretions provide a plentiful supply glucose, lipids, glycoproteins growth factors stimulate rapid proliferation differentiation villous trophoblast. Furthermore, gland organoids indicates expression secretion these products are upregulated following sequential exposure oestrogen, progesterone trophoblastic decidual hormones, in particular prolactin. Hence, feed-forward signalling dialogue proposed among trophoblast, decidua enables own development, independent Many common complications pregnancy represent spectrum disorders associated with deficient trophoblast proliferation. Increasing suggests this mirrored one impaired decidualization, potentially compromising histotroph through diminished prolactin reduced function. Optimizing wellbeing prior conception may therefore help prevent complications, such as miscarriage, restriction pre-eclampsia.

Language: Английский

Citations

42

Organoid co-culture model of the human endometrium in a fully synthetic extracellular matrix enables the study of epithelial-stromal crosstalk DOI Creative Commons

Juan S. Gnecco,

Alexander Brown,

Kira Buttrey

et al.

Med, Journal Year: 2023, Volume and Issue: 4(8), P. 554 - 579.e9

Published: Aug. 1, 2023

Language: Английский

Citations

41

Endocrine disorders and fertility and pregnancy: An update DOI Creative Commons
Anna Bednarska‐Czerwińska, Nikola Zmarzły, Emilia Morawiec

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 17, 2023

It is estimated that more and couples suffer from fertility pregnancy maintenance disorders. associated with impaired androgen secretion, which influenced by many factors, ranging genetic to environmental. also important remember disorders can result abnormal anatomy of the reproductive male female organ (congenital uterine anomalies – septate, unicornuate, bicornuate uterus; acquired defects uterus structure fibroids, polyps, hypertrophy), disturbed hormonal cycle obstruction fallopian tubes resulting presence adhesions due inflammation, endometriosis, surgery, rhythm menstrual bleeding, concentration hormones. There are relationships between endocrine organs, leading a chain reaction when one them fails function properly. Conditions in immune system involved, including infections autoimmune diseases, affect fertility. The form treatment depends on infertility duration patient’s age. includes ovulation stimulation clomiphene citrate or gonadotropins, metformin use, weight loss interventions. Since so different factors fertility, it correctly diagnose what causing problem modify regimen if necessary. This review describes disturbances hormone secretion individual organs context pregnancy.

Language: Английский

Citations

32

Natural Killer Cells in the Human Uterine Mucosa DOI Creative Commons
Victoria Male, Ashley Moffett

Annual Review of Immunology, Journal Year: 2023, Volume and Issue: 41(1), P. 127 - 151

Published: Jan. 11, 2023

The presence of granulated lymphocytes in the human uterine mucosa, known as decidua during pregnancy, or endometrium otherwise, was first noted nineteenth century, but it not until 1990 that these cells were identified a type natural killer (NK) cell. From outset, NK (uNK) found to be less cytotoxic than their circulating counterparts, peripheral (pNK) cells. Recently, unbiased approaches have defined three subpopulations uNK cells, all which cluster separately from pNK Here, we review history research into including ability interact with placental extravillous trophoblast and potential role regulating implantation. We go on more recent advances focus cell development heterogeneity defend against infection mediate memory effects. Finally, consider how better understanding could leveraged future improve outcomes pregnancy for mothers babies.

Language: Английский

Citations

26