Symbiont acquisition strategies in post-settlement stages of two co-occurring deep-sea Rimicaris shrimp.

Authorea (Authorea), Journal Year: 2024, Volume and Issue: unknown

Published: April 14, 2024

At deep-sea hydrothermal vents, deprived of light, most living communities are fuelled by chemosynthetic microorganisms. These can form symbiotic associations with metazoan hosts, which then called holobionts. Among these, two endemic shrimp the Mid-Atlantic Ridge (MAR), Rimicaris exoculata and chacei colonized dense diversified in their cephalothoracic cavity digestive system. Although both harbor similar communities, they exhibit widely different population densities, distribution patterns at small scale diet, as well differences post-settlement morphological modifications leading to adult stage. contrasting biological traits may be linked development success. Consequently, key questions related acquisition symbionts holobiont still open. Here we examined juveniles R. from TAG Snake Pit using 16S metabarcoding identify lineages present each juvenile In addition, highlighted abundance microorganisms stage Fluorescence situ Hybridization (FISH) Scanning Electron Microscopy (SEM). For first time, Candidatus Microvillispirillaceae (midgut tube), Foregutplasma rimicarensis BG2-rimicarensis (foregut) were identified late stages. However, these absent early stages, coincides for midgut tube our observations an immature tissue, devoid microvilli. Conversely, earliest stages species overall diversities those adults. results suggest symbiont dynamics between system, also involve distinct transmission mechanisms.

Language: Английский

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Transcriptomic responses of Mediterranean sponges upon encounter with symbiont microbial consortia

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: July 7, 2024

Abstract Background Sponges (phylum Porifera) constantly interact with microbes. They graze on microbes from the water column by filter-feeding and they harbor symbiotic partners within their bodies. In experimental setups, sponges take up symbionts at lower rates compared seawater This suggests that have capacity to differentiate between preferentially in non-symbiotic microbes, although underlying mechanisms of discrimination are still poorly understood. Genomic studies showed that, other animal groups, present an extended repertoire immune receptors, particular NLRs, SRCRs, GPCRs, a handful experiments regulate expression these receptors upon encounter microbial elicitors. We hypothesize may rely differential diverse poriferan sense different consortia while filter-feeding. To test this, we characterized transcriptomic response two sponge species, Aplysina aerophoba Dysidea avara , incubation extracted A. comparison The were sampled after 1 h, 3 5 h for RNA-Seq gene analysis. Results D. incubated -symbionts regulated genes related immunity, ubiquitination, signaling. Within set differentially-expressed identified families Nucleotide Oligomerization Domain (NOD)-Like Receptors (NLRs). These results represent first evidence types NLRs involved sponge. contrast, its own comparatively fewer lacked encoding receptors. Conclusion Our work that: (i) exposure imply “fine-tuning” baseline as result interaction (ii) encounters varied probably due species-specific characteristics or host’s traits, (iii) belonging NLR-like played role whether food bacteria. regulation provides further potential invertebrate host-microbe interactions. study responses exemplifies how investigating groups broadens our knowledge evolution specificity symbiosis.

Language: Английский

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Symbiont Acquisition Strategies in Post‐Settlement Stages of Two Co‐Occurring Deep‐Sea Rimicaris Shrimp

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(11)

Published: Nov. 1, 2024

At deep-sea hydrothermal vents, deprived of light, most living communities are fueled by chemosynthetic microorganisms. These can form symbiotic associations with metazoan hosts, which then called holobionts. Among these, two endemic co-occurring shrimp the Mid-Atlantic Ridge (MAR), Rimicaris exoculata and chacei colonized dense diversified in their cephalothoracic cavity digestive system. Although both harbor similar communities, they exhibit widely different population densities, distribution patterns at small scale diet, as well differences post-settlement morphological modifications leading to adult stage. contrasting biological traits may be linked development success. Consequently, key questions related acquisition three organs still open. Here we examined juveniles R. from TAG Snake Pit using 16S metabarcoding identify lineages present each juvenile In addition, highlighted abundance microorganisms stage Fluorescence situ Hybridization (FISH) Scanning Electron Microscopy (SEM). For first time, Candidatus Microvillispirillaceae family Rimicarispirillum spp. (midgut tube), Foregutplasma rimicarensis BG2-rimicarensis (foregut) were identified late stages. However, these absent early stages, coincides for midgut tube our observations an immature tissue, devoid microvilli. Conversely, earliest stages species overall diversities those adults. results suggest symbiont dynamics between system, also involve distinct transmission mechanisms.

Language: Английский

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Symbiosis of disciplines: how can developmental biologists join conservationists in sustaining and restoring earth's biodiversity?

Development, Journal Year: 2022, Volume and Issue: 149(13)

Published: July 1, 2022

ABSTRACT What can developmental biology contribute toward mitigating the consequences of anthropogenic assaults on environment and climate change? In this Spotlight article, we advocate a that takes seriously Lynn Margulis' claim ‘the is part body’. We believe to be pre-condition for playing important roles in conservation environmental restoration. need forge holobiont – multi-genomic physiologically integrated organism also functional biome. To end, highlight how needs explore more deeply interactions between developing organisms, their chemical, physical biotic environments.

Language: Английский

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Editorial: Interaction between marine invertebrates and symbiotic microbes in a changing environment: Community structure and ecological functions

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 9

Published: Jan. 12, 2023

EDITORIAL article Front. Mar. Sci., 12 January 2023Sec. Microbial Symbioses Volume 9 - 2022 | https://doi.org/10.3389/fmars.2022.1128906

Language: Английский

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Microbes as part of ancestral neuronal circuits: Bacterial produced signals affect neurons controlling eating behavior inHydra

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 29, 2023

Summary Although recent studies indicate the impact of microbes on central nervous systems and behavior, it remains unclear how relationship between functionality system, behavior microbiota arise. We studied eating Hydra, a host that has simple system low-complexity microbiota. To identify neuronal subpopulations involved, we used subpopulation specific cell ablation calcium imaging. The role was uncovered by reducing diversity natural Here, demonstrate different are functioning together to control behavior. participates in since germ-free or mono-colonized animals have drastic difficulties mouth opening. This restored adding full complement In summary, provide mechanistic explanation is controlled Hydra can affect circuit. Highlights - Multiple modules their networks complex an animal lacking system. Its associated participate these circuits influence Disorganization negatively impacts this Glutamate evolutionary ancient interkingdom language.

Language: Английский

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Microbes as manipulators of developmental life-history

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 6, 2024

ABSTRACT Marine invertebrates mainly reproduce by energy-poor eggs that develop into feeding larvae or energy-rich non-feeding 1–4 . Transitions between these reproductive strategies have been studied in detail 5–7 , yet the evolutionary factor(s) responsible for switches remains elusive. Here, we use theoretical models to show microbes with capacity manipulate host reproduction are one possible factor. We report microbial manipulators create a sperm-limited environment selects larger shifting host’s sex ratio towards female dominance and, as result, serve driver of transitions developmental life-history marine invertebrates. Loss manipulator can then recover ancestral life-history. also document more than dozen genera from throughout world’s oceans fit framework microbe-induced switch predominate strategies. anticipate yet-to-be appreciated influence on find it paramount understand if occur without well underlying mechanisms manipulations convergent terrestrial systems.

Language: Английский

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A strong priority effect in the assembly of a specialized insect-microbe symbiosis

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 27, 2024

Abstract Microbial community assembly is determined in part by interactions between taxa that colonize ecological niches available within habitat patches. The outcomes of these interactions, and extension the trajectory assembly, can display priority effects - dependency on order which first occupy niches. underlying mechanisms phenomena vary from system to are often not well resolved. Here, we characterize colonization squash bug ( Anasa tristis ) bacterial symbionts genus Caballeronia , using pairs strains known strongly compete during host colonization, as isogenic thus functionally identical. By introducing symbiont into individual bugs a sequential manner, show within-host populations established colonist extremely resistant invasion, regardless strain identity competitive interactions. knocking down population an initial with antibiotics, further success second still diminished even when space symbiotic organ physically accessible for colonization. We propose paradigm resident exclude subsequent infections manipulating environment, partially but exclusively eliciting tissue remodeling organ. Importance Host-associated microbial communities underpin critical ecosystem processes human health, their ability do so turn various shape composition. While natural selection acts competing genotypes species manner determines differ depending sequence establish community. document this phenomenon, effect, experimental North American insect pest, its betaproteobacterial . Our study demonstrates how stark, strain-level variation emerge specialized host-microbe symbioses simply through differences host. Understanding mechanistic drivers structure host-associated microbiomes highlight both pitfalls opportunities engineering constituent societal benefit.

Language: Английский

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Accessible Autonomous Vehicles as Symbiotic Autonomous Systems for Users with Disabilities: Preliminary Design Guidelines

Published: May 15, 2024

In biology and life sciences, symbiosis refers to a close interdependent relationship between two biological species who live in mutually supportive, cooperative way. this paper, we apply the concept of problem autonomous vehicle accessibility. We have reimagined accessible as symbiotic system that enables user with disabilities function independently ways neither could. present six guidelines for designing Symbiotic Autonomous Vehicles (SyAVs) explicitly emphasize perhaps unique needs SyAV users disabilities. This work contributes scientific literature by discussing SyAVs from perspectives persons provides guidance designers advanced systems.

Language: Английский

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Facultatively intrabacterial localization of a planthopper endosymbiont as an adaptation to its vertical transmission

mSystems, Journal Year: 2024, Volume and Issue: 9(7)

Published: June 27, 2024

Transovarial transmission is the most reliable way of passing on essential nutrient-providing endosymbionts from mothers to offspring. However, not all endosymbiotic microbes follow complex path through female host tissues oocytes their own. Here, we demonstrate an unusual strategy adopted by one planthopper

Language: Английский

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