Synaptic connectome of a neurosecretory network in the Drosophila brain DOI Open Access
Theresa H. McKim, Jayati Gera,

Ariana Julia B. Gayban

et al.

Published: Nov. 14, 2024

Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Language: Английский

Cellular and molecular organization of the Drosophila foregut DOI Creative Commons
Haolong Zhu, William B. Ludington, Allan C. Spradling

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(11)

Published: March 5, 2024

The animal foregut is the first tissue to encounter ingested food, bacteria, and viruses. We characterized adult Drosophila using transcriptomics better understand how it triages consumed items for digestion or immune response manages resources. Cell types were assigned validated GFP-tagged Gal4 reporter lines. Foregut-associated neuroendocrine cells play a major integrative role by coordinating gut activity with nutrition, microbiome, circadian cycles; some express clock genes. Multiple epithelial cell comprise proventriculus, central organ that secretes peritrophic matrix (PM) lining gut. Analyzing synthesizing individual PM layers revealed abundant mucin production close enterocytes, similar mammalian intestinal mucosa. esophagus salivary gland secreted proteins likely line esophageal surface, of which may generate commensal niche housing specific microbiome species. Overall, our results imply coordinates dietary sensing, hormonal regulation, immunity in manner has been conserved during evolution.

Language: Английский

Citations

9
Neuropeptidergic regulation of insect diapause by the circadian clock DOI Creative Commons
Charlotte Helfrich‐Förster

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101198 - 101198

Published: April 6, 2024

Diapause is an endocrine-mediated strategy used by insects to survive seasons of adverse environmental conditions. Insects living in temperate zones are regularly exposed such conditions the form winter. To winter, they must prepare for it long before arrives. A reliable indicator impending winter shortening day length. measure length, need their circadian clock as internal time reference. In this article, I provide overview current state knowledge on neuropeptides that link diapause inducing hormonal brain centers.

Language: Английский

Citations

8
Synaptic connectome of a neurosecretory network in the Drosophila brain DOI Creative Commons
Theresa H. McKim, Jayati Gera,

Ariana Julia B. Gayban

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 29, 2024

Abstract Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Language: Английский

Citations

5
Gut-to-brain regulation of Drosophila aging through neuropeptide F, insulin, and juvenile hormone DOI Creative Commons
Jiangtian Chen, Marcela Nouzová, Fernando G. Noriega

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(43)

Published: Oct. 16, 2024

Dietary restriction (DR) slows aging in many animals, while some cases, the sensory signals from diet alone are sufficient to retard or accelerate lifespan. The digestive tract is a candidate location sense nutrients, where neuropeptides secreted by enteroendocrine cells (EEC) produce systemic response food. Here, we measure how Drosophila neuropeptide F (NPF) into adult circulation EEC and find that specific differentially respond dietary sugar yeast. Female lifespan increased when gut NPF genetically depleted, this manipulation blunt longevity benefit conferred DR. Depletion of receptors at insulin-producing neurons brain also increases female lifespan, consistent with observations loss decreases neuronal insulin secretion. repressing reversed treating adults juvenile hormone (JH) analog. JH produced corpora allata, inhibition receptor tissue titer extends both males females, restored wild type Overall, modulate through interorgan communication mediated gut–brain–corpora allata axis, impacts its control titer. These data suggest consider human incretins their analogs, which used treat obesity diabetes, may impact aging.

Language: Английский

Citations

4
A Head‐Specific Transcriptomic Study Reveals Key Regulatory Pathways for Winter Diapause in the Mosquito Culex pipiens DOI Open Access
Prabin Dhungana, Xueyan Wei, David Sung Yong Kang

et al.

Archives of Insect Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 118(2)

Published: Feb. 1, 2025

ABSTRACT The primary vector of the West Nile virus, Culex pipiens , undergoes reproductive dormancy during adverse winter season. While our current understanding has mainly focused on cellular signals and phenotypic shifts occurring at a global scale diapause, information tissue‐specific transcriptomic changes remains limited. This knowledge gap is major challenge in interpreting regulatory mechanisms tissue level. To address this, present work utilized RNA‐seq technology to investigate transcriptional head that house brain crucial endocrinal organs such as corpora allata. We obtained RNA samples from heads diapausing nondiapausing female mosquitoes two specific time intervals, ZT0 ZT16, then subjected them sequencing. Our results revealed differences differentially expressed genes between diapause non‐diapause highlighting diel variations gene expression. also selected twelve associated with phenotype examined transcript abundance six different points over 24 h. qRT‐PCR analysis showed similar up‐ downregulation transcripts nondiapause phenotypes thus validating RNA‐seq. In summary, study identified new differentiation their expression, potentially linking photoperiod seasonal insects. newly presented will significantly advance head‐specific for insect diapause.

Language: Английский

Citations

0
What Drosophila can tell us about state-dependent peptidergic signaling in insects DOI Creative Commons
Dick R. Nässel

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104275 - 104275

Published: Feb. 1, 2025

Language: Английский

Citations

0
Neuropeptide‐Dependent Spike Time Precision and Plasticity in Circadian Output Neurons DOI Creative Commons
Bryan Chong, Vipin Kumar, Dieu Linh Nguyen

et al.

European Journal of Neuroscience, Journal Year: 2025, Volume and Issue: 61(5)

Published: March 1, 2025

ABSTRACT Circadian rhythms influence various physiological and behavioral processes such as sleep–wake cycles, hormone secretion, metabolism. In Drosophila , an important set of circadian output neurons is called pars intercerebralis (PI) neurons, which receive input from specific clock DN1. These DN1 can further be subdivided into functionally anatomically distinctive anterior (DN1a) posterior (DN1p) clusters. The neuropeptide diuretic hormones 31 (Dh31) 44 (Dh44) are the insect neuropeptides known to activate PI control activity rhythms. However, neurophysiological basis how Dh31 Dh44 affect neural coding mechanisms underlying sleep in not well understood. Here, we identify Dh31/Dh44‐dependent spike time precision plasticity neurons. We first find that a mixture enhanced firing compared application alone alone. next synthesized affects membrane potential dynamics precise timing neuronal through their synergistic interaction, possibly mediated by calcium‐activated potassium channel conductance. Further, characterize Dh31/Dh44 enhances postsynaptic potentials Together, these results suggest multiplexed neuropeptide‐dependent .

Language: Английский

Citations

0
Finishing the egg DOI
Celeste A. Berg, Matthew Sieber, Jianjun Sun

et al.

Genetics, Journal Year: 2023, Volume and Issue: 226(1)

Published: Nov. 22, 2023

Gamete development is a fundamental process that highly conserved from early eukaryotes to mammals. As germ cells develop, they must coordinate dynamic series of cellular processes support growth, cell specification, patterning, the loading maternal factors (RNAs, proteins, and nutrients), differentiation structures enable fertilization ensure embryonic survival, other make functional oocyte. To achieve these goals, integrate complex milieu environmental developmental signals produce fertilizable eggs. Over past 50 years, Drosophila oogenesis has risen forefront as system interrogate sophisticated mechanisms drive oocyte development. Studies in have defined control meiosis, protect genome integrity, facilitate mRNA trafficking, nutrients. Work this provided key insights into establish egg chamber polarity patterning well ovulation activation. Using power genetics, field begun define molecular stresses nutrient availability with Importantly, majority reproductive are throughout evolution, many play critical roles somatic tissues well. In chapter, we summarize recent progress several areas impact ovulation. First, discuss storage trafficking during maturation vitellogenesis. Second, examine regulate follicle how impacts construction shell establishment polarity. Finally, regulatory ovulation, activation, successful fertilization.

Language: Английский

Citations

10
Impact of diapause on the cuticular hydrocarbons and physiological status of Hippodamia variegata DOI Creative Commons

Mahsa Khabir,

Hamzeh Izadi,

Kamran Mahdian

et al.

Journal of Insect Science, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 1, 2024

Abstract The spotted amber ladybird, Hippodamia variegata (Goeze) (Coleoptera: Coccinellidae), is known to be a potent predator of aphids, psyllids, whiteflies, mealybugs, and some butterfly species. This ladybeetle overwinters in the diapausing adult stage. current study aimed evaluate impact diapause on energy resources cuticular hydrocarbons (CHCs) female ladybeetle, specifically comparing changes glycogen, lipid, protein contents, CHCs profile non-diapausing adults. In this study, gas chromatography-mass was used analyze whole-body extracts beetles. Results showed no significant differences between amount contents ladybeetle. H. consisted 24 categorized into 2 groups: linear aliphatic (n-alkanes) methyl-branched (17 molecules), as well unsaturated cyclic compounds (7 molecules). n-alkanes, with 14 compounds, were identified primary constituents Six molecules common beetles, 5 exclusive 13 Moreover, we noted difference quantity quality beetles synthesizing more longer chains. disparity CHC profiles concluded an adaptation survive harsh environmental conditions during diapause.

Language: Английский

Citations

3
A high-protein diet-responsive gut hormone regulates behavioral and metabolic optimization in Drosophila melanogaster DOI Creative Commons
Yuto Yoshinari, T. Nishimura, Taishi Yoshii

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 30, 2024

Protein is essential for all living organisms; however, excessive protein intake can have adverse effects, such as hyperammonemia. Although mechanisms responding to deficiency are well-studied, there a significant gap in our understanding of how organisms adaptively suppress intake. In the present study, utilizing fruit fly, Drosophila melanogaster, we discover that peptide hormone CCHamide1 (CCHa1), secreted by enteroendocrine cells response high-protein diet (HPD), vital suppressing overconsumption protein. Gut-derived CCHa1 received small subset enteric neurons produce short neuropeptide F, thereby modulating protein-specific satiety. Importantly, impairment CCHa1-mediated gut-enteric neuronal axis results ammonia accumulation and shortened lifespan under HPD conditions. Collectively, findings unravel crosstalk gut pathways orchestrate physiological responses prevent adapt dietary overload.

Language: Английский

Citations

3