Synaptic connectome of a neurosecretory network in the Drosophila brain DOI Open Access
Theresa H. McKim, Jayati Gera,

Ariana Julia B. Gayban

et al.

Published: Nov. 14, 2024

Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Language: Английский

Creation of Knock-In Alleles of Insulin Receptor Tagged by Fluorescent Proteins mCherry or EYFP in Fruit Fly Drosophila melanogaster DOI

Ayano Moriya,

Kei Otsuka,

Riku Naoi

et al.

ZOOLOGICAL SCIENCE, Journal Year: 2024, Volume and Issue: 41(2)

Published: March 5, 2024

The insulin/insulin-like growth factor-like signaling (IIS) pathway is highly conserved across metazoans and regulates numerous physiological functions, including development, metabolism, fecundity, lifespan. insulin receptor (InR), a crucial membrane in the IIS pathway, known to be ubiquitously expressed various tissues, albeit at generally low levels, its subcellular localization remains incompletely characterized. In this study, we employed CRISPR-mediated mutagenesis fruit fly Drosophila create knock-in alleles of InR tagged with fluorescent proteins (InR::mCherry or InR::EYFP). By inserting coding sequence mCherry EYFP near end endogenous gene, could trace natural protein through their fluorescence. As an example, investigated epithelial cells male accessory gland (AG), internal reproductive organ, identified two distinct patterns InR::mCherry localization. young AG, accumulated on basal plasma between cells, whereas mature it exhibited intracellular as multiple puncta, indicating endocytic recycling during cell growth. AG senescence accelerated by mutation Diuretic hormone 31 (Dh31), presence puncta was more pronounced compared wild type. These findings raise expectations for utility newly created InR::mCherry/EYFP studying precise expression levels InR. Furthermore, fluorescently allele approach can extended investigate other receptors abundance, facilitating direct examination true

Language: Английский

Citations

0
Characterization of pre-diapause phase in the northern Drosophila species D. ezoana DOI
Koustubh M. Vaze, Giulia Manoli, Charlotte Helfrich‐Förster

et al.

Journal of Comparative Physiology A, Journal Year: 2024, Volume and Issue: 210(6), P. 901 - 908

Published: June 25, 2024

Language: Английский

Citations

0
Regional specialization, polyploidy, and seminal fluid transcripts in the Drosophila female reproductive tract DOI Creative Commons
Rachel C. Thayer, Elizabeth S. Polston,

Jixiang Xu

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(44)

Published: Oct. 25, 2024

Sexual reproduction requires the choreographed interaction of female cells and molecules with sperm seminal fluid. In internally fertilizing animals, these interactions are managed by specialized tissues within reproductive tract (FRT), such as a uterus, glands, storage organs. However, somatic remain understudied, hindering insight into molecular that support fertility. Here, we report identification, characterization, analysis cell types throughout FRT in premier

Language: Английский

Citations

0
Synaptic connectome of a neurosecretory network in the Drosophila brain DOI Open Access
Theresa H. McKim, Jayati Gera,

Ariana Julia B. Gayban

et al.

Published: Nov. 14, 2024

Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Language: Английский

Citations

0