The conserved protective cyclic AMP-phosphodiesterase function PDE4B is expressed in the adenoma and adjacent normal colonic epithelium of mammals and silenced in colorectal cancer DOI Creative Commons

Jennifer K. Pleiman,

Amy A. Irving,

Zhishi Wang

et al.

PLoS Genetics, Journal Year: 2018, Volume and Issue: 14(9), P. e1007611 - e1007611

Published: Sept. 6, 2018

Conservation over three mammalian genera—the mouse, rat, and human—has been found for a subset of the transcripts whose level differs between adenoma normal epithelium colon. Pde4b is one triply conserved enhanced both in colonic epithelium, especially adjacent to adenomas. It encodes phosphodiesterase PDE4B, specific cAMP. Loss PDE4B function ApcMin/+ mouse leads significant increase number Similarly, Pde4b-deficient mice are hypersensitive treatment by inflammatory agent DSS, becoming moribund soon after treatment. These observations imply that protects against ApcMin-induced adenomagenesis lethality. The paradoxical enhancement transcript versus this inferred protective can be rationalized feedback model which first activated early oncogenic stress involving cAMP then, as reported frank human colon cancer, inactivated epigenetic silencing.

Language: Английский

Application of genome editing technologies in rats for human disease models DOI
Kazuto Yoshimi, Tomoji Mashimo

Journal of Human Genetics, Journal Year: 2017, Volume and Issue: 63(2), P. 115 - 123

Published: Nov. 20, 2017

Language: Английский

Citations

17
Morin‐dependent inhibition of low molecular weight protein tyrosine phosphatase (LMW‐PTP) restores sensitivity to apoptosis during colon carcinogenesis: Studies in vitro and in vivo, in an Apc‐driven model of colon cancer DOI
Giulia Lori, Paolo Paoli, Angelo Pietro Femia

et al.

Molecular Carcinogenesis, Journal Year: 2018, Volume and Issue: 58(5), P. 686 - 698

Published: Dec. 24, 2018

LMW‐PTP has been associated with the development of colorectal cancer (CRC) and resistance to chemotherapy in cells. To clarify its role vivo, we studied expression Pirc rats (F344/NTac‐Apc am1137 ), genetically prone CRC resistant apoptosis. In morphologically normal mucosa (NM) rats, a dramatic over‐expression was found compared wt (about 60 times higher). Moreover, levels further increase spontaneously developed colon tumors. understand if how affects apoptosis, cell lines, sensitive (HT29 HCT‐116), or (HT29R, HCT116R) 5‐Fluorouracil (5‐FU): cells over‐express LMW‐PTP. When were challenged morin, polyphenol inhibiting LMW‐PTP, fast dose‐related down‐regulation observed. 5‐FU morin co‐treatment dramatically decreased viability, increased significantly impaired self‐renewal ability all lines have studied. Similarly, observed that, one‐week administration (50 mg/kg) down‐regulated restored apoptotic response NM. Finally, for longer period led significant reduction precancerous lesions, together Taken together, these results document involvement process vitro vivo. Morin treatment may be envisaged as system sensitivity prevent carcinogenesis.

Language: Английский

Citations

17
Expression profile of long non-coding RNAs in colorectal cancer: A microarray analysis DOI Open Access
Jia Luo, Luning Xu,

Yigui Jiang

et al.

Oncology Reports, Journal Year: 2016, Volume and Issue: 35(4), P. 2035 - 2044

Published: Feb. 1, 2016

Language: Английский

Citations

15
Characterization of Optical Coherence Tomography Images for Colon Lesion Differentiation under Deep Learning DOI Creative Commons
Cristina L. Saratxaga,

Jorge Bote,

Juan Francisco Ortega Morán

et al.

Applied Sciences, Journal Year: 2021, Volume and Issue: 11(7), P. 3119 - 3119

Published: April 1, 2021

(1) Background: Clinicians demand new tools for early diagnosis and improved detection of colon lesions that are vital patient prognosis. Optical coherence tomography (OCT) allows microscopical inspection tissue might serve as an optical biopsy method could lead to in-situ treatment decisions; (2) Methods: A database murine (rat) healthy, hyperplastic neoplastic colonic samples with more than 94,000 images was acquired. methodology includes a data augmentation processing strategy deep learning model automatic classification (benign vs. malignant) OCT is presented validated over this dataset. Comparative evaluation performed both individual B-scan C-scan volumes; (3) Results: trained evaluated the proposed using six different splits present statistically significant results. Considering this, 0.9695 (±0.0141) sensitivity 0.8094 (±0.1524) specificity were obtained when images. On other hand, 0.9821 (±0.0197) 0.7865 (±0.205) achieved made considering all in whole volume; (4) Conclusions: The based on showed great potential characterization polyps future development paradigm.

Language: Английский

Citations

14
The conserved protective cyclic AMP-phosphodiesterase function PDE4B is expressed in the adenoma and adjacent normal colonic epithelium of mammals and silenced in colorectal cancer DOI Creative Commons

Jennifer K. Pleiman,

Amy A. Irving,

Zhishi Wang

et al.

PLoS Genetics, Journal Year: 2018, Volume and Issue: 14(9), P. e1007611 - e1007611

Published: Sept. 6, 2018

Conservation over three mammalian genera—the mouse, rat, and human—has been found for a subset of the transcripts whose level differs between adenoma normal epithelium colon. Pde4b is one triply conserved enhanced both in colonic epithelium, especially adjacent to adenomas. It encodes phosphodiesterase PDE4B, specific cAMP. Loss PDE4B function ApcMin/+ mouse leads significant increase number Similarly, Pde4b-deficient mice are hypersensitive treatment by inflammatory agent DSS, becoming moribund soon after treatment. These observations imply that protects against ApcMin-induced adenomagenesis lethality. The paradoxical enhancement transcript versus this inferred protective can be rationalized feedback model which first activated early oncogenic stress involving cAMP then, as reported frank human colon cancer, inactivated epigenetic silencing.

Language: Английский

Citations

15