ENST00000438158 aids ultrasound for predicting lymph node metastasis and inhibits migration and invasion of papillary thyroid carcinoma cells DOI Open Access
Hui Liu, Yixin Shi, Jia Zhan

et al.

Drug Discoveries & Therapeutics, Journal Year: 2022, Volume and Issue: 17(1), P. 26 - 36

Published: Oct. 18, 2022

Cervical lymph node metastasis (CLNM) of papillary thyroid carcinoma (PTC) is directly associated with clinical management and prognosis. In this study, we aimed to evaluate the value conventional ultrasound (US) combined ENST00000438158 in predicting CLNM PTC. Fourty-nine PTC patients underwent US examination US-guided fine needle aspiration (FNA). expression FNA cytological specimens cell lines was detected using real-time reverse transcription polymerase chain reaction (qRT-PCR). The role proliferation, migration, invasion, apoptosis, cycle cells investigated by Cell Counting Kit-8 (CCK8) clone formation experiments, transwell assay, flow cytometry, respectively. Calcification, capsule contact, low were independently (all p < 0.05). combination multiple features more valuable than a single feature Adding ENST0000438158 greatly improved differentiation or without CLNM. conclusion, potential molecular marker for highly

Language: Английский

piR‐823 contributes to colorectal tumorigenesis by enhancing the transcriptional activity of HSF1 DOI Creative Commons
Jie Yin, Xiaoyu Jiang, Wei Qi

et al.

Cancer Science, Journal Year: 2017, Volume and Issue: 108(9), P. 1746 - 1756

Published: June 15, 2017

Piwi‐interacting RNA s (pi s), a novel class of small non‐coding s, were first discovered in germline cells and are thought to silence transposons spermatogenesis. Recently, pi have also been identified somatic tissues, aberrant expression tumor tissues may be implicated carcinogenesis. However, the function piR‐823 colorectal cancer ( CRC ) remains unclear. Here, we found that was significantly upregulated compared with its adjacent tissues. Inhibition suppressed cell proliferation, arrested cycle G1 phase induced apoptosis lines HCT 116 DLD ‐1, whereas overexpression promoted proliferation normal colonic epithelial line FHC . Interestingly, repressed heat shock protein HSP 27, 60, 70. Furthermore, elevated partially abolished effect on apoptosis. In addition, further demonstrated increased transcriptional activity HSF 1, common transcription factor by binding 1 promoting phosphorylation at Ser326. Our study reveals plays tumor‐promoting role upregulating suggests as potential therapeutic target for

Language: Английский

Citations

120
LncRNAs with miRNAs in regulation of gastric, liver, and colorectal cancers: updates in recent years DOI
Chit Tam, Jack Ho Wong, Stephen Kwok‐Wing Tsui

et al.

Applied Microbiology and Biotechnology, Journal Year: 2019, Volume and Issue: 103(12), P. 4649 - 4677

Published: May 6, 2019

Language: Английский

Citations

116
LncRNA AB073614 regulates proliferation and metastasis of colorectal cancer cells via the PI3K/AKT signaling pathway DOI
Yanan Wang,

Haoyu Kuang,

Jinfang Xue

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2017, Volume and Issue: 93, P. 1230 - 1237

Published: July 20, 2017

Language: Английский

Citations

65
LncRNAs in DNA damage response and repair in cancer cells DOI Open Access
Min Su, Heran Wang,

Wenxiang Wang

et al.

Acta Biochimica et Biophysica Sinica, Journal Year: 2018, Volume and Issue: 50(5), P. 433 - 439

Published: Feb. 22, 2018

In order to maintain integrity of the genome, eukaryotic cells develop a complex DNA damage/ repair response network, which can induce cell cycle arrest, apoptosis, or repair.Chemo-and radiation therapies, act primarily through induction damage, are most commonly used therapies for cancer.Impairment in damage and system that protect from persistent affect therapeutic efficacy cancer.To date, accumulating evidence has suggested long non-coding RNAs (lncRNAs) involved regulation damage/repair network.LncRNAs have been demonstrated be master regulators genome at transcriptional post-transcriptional levels play key role many physiological pathological processes cells.In this review, we will discuss function lncRNAs regulating cellular damage.

Language: Английский

Citations

63
LncRNA SLCO4A1-AS1 promotes colorectal cancer cell proliferation by enhancing autophagy via miR-508-3p/PARD3 axis DOI Creative Commons
Zhaozhi Wang, Jianjun Jin

Aging, Journal Year: 2019, Volume and Issue: 11(14), P. 4876 - 4889

Published: July 16, 2019

Aberrant expressions of various long non-coding RNAs (lncRNAs) have been involved in the progression and pathogenesis carcinomas. However, expression biological function SLCO4A1-AS1 colorectal cancer (CRC) remain poorly understood. Gain- loss-of-function assays were applied to determine roles autophagy CRC progression. qRT-PCR situ hybridization (ISH) results showed that was positively associated with PARD3 tissues. In vitro vivo studies revealed knockdown repressed cytoprotective as assayed by transmission electron microscopy (TEM), inhibited cell proliferation directly targeting partition-defective 3 (PARD3). Mechanistically, acted a sponge miR-508-3p, leading upregulation promotion proliferation. The current study demonstrates SLCO4A1-AS1/miR-508-3p/PARD3/autophagy pathway play critical role proliferation, might provide novel targets for developing therapeutic strategies CRC.

Language: Английский

Citations

51
Long non-coding RNA FBXL19-AS1 plays oncogenic role in colorectal cancer by sponging miR-203 DOI
Bo Shen, Yuan Yuan, Yan Zhang

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2017, Volume and Issue: 488(1), P. 67 - 73

Published: May 4, 2017

Language: Английский

Citations

43
Temporal expression and functional analysis of long non‐coding RNAs in colorectal cancer initiation DOI Creative Commons
Lei Dai, Junshu Li,

Zhexu Dong

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2019, Volume and Issue: 23(6), P. 4127 - 4138

Published: March 28, 2019

Long non-coding RNAs (lncRNAs) have potential applications in clinical diagnosis and targeted cancer therapies. However, the expression profile of lncRNAs colorectal (CRC) initiation is still unclear. In this study, profiles mRNAs were determined by microarray at specific tumour stages an AOM/DSS-induced primary colon model. The temporal was analysed K-means clustering. Additionally, weighted correlation network analysis (WGCNA) gene ontology performed to construct co-expression networks establish functions identified mRNAs. Our results suggested that 4307 5798 are deregulated during CRC initiation. These differential genes (DEGs) exhibited a clear with stage WGCNA series hub involved regulating cell stemness, inflammation, oxidative stress response death each stage. Among them, lncRNA H19 up-regulated tumours correlated poor patient prognosis. Collectively, we been first demonstrate function provide novel therapy targets for CRC.

Language: Английский

Citations

25
Genome-wide analysis of long non-coding RNA expression and function in colorectal cancer DOI Creative Commons

Fangyuan Jing,

Huicheng Jin,

Yingying Mao

et al.

Tumor Biology, Journal Year: 2017, Volume and Issue: 39(5), P. 101042831770365 - 101042831770365

Published: May 1, 2017

Long non-coding RNAs (lncRNAs) are widely transcribed in the genome, but their expression profile and roles colorectal cancer not well understood. The aim of this study was to investigate long RNA look for potential diagnostic biomarkers cancer. microarray applied global Gene ontology Kyoto Encyclopedia Genes Genomes pathway analyses were performed using standard enrichment computational methods. levels selected validated by quantitative reverse transcription polymerase chain reaction. relationship between clinicopathological characteristics patients assessed. Coexpression carried out find coexpressed genes differentially expressed RNAs, followed gene analysis predict possible role carcinogenesis. In study, a total 1596 transcripts 1866 messenger dysregulated tumor tissues compared with paired normal tissues. top upregulated CCAT1, UCA1, RP5-881L22.5, NOS2P3, BC005081 downregulated AK055386, AC078941.1, RP4-800J21.3, RP11-628E19.3, RP11-384P7.7. UCA1 significantly colon cancer, AK055386 dimension <5 cm. Functional prediction showed that both coexpress cell cycle related RNAs. current provided novel insights into predicted Among found be associated anatomic site, size. Further functional investigations molecular mechanisms warranted clarify

Language: Английский

Citations

19
The long non-coding RNA ENST00000547547 reduces 5-fluorouracil resistance of colorectal cancer cells via competitive binding to microRNA-31 DOI Open Access
Jian Li, Xiaorong Li,

Chao‐Qun Cen

et al.

Oncology Reports, Journal Year: 2017, Volume and Issue: unknown

Published: Nov. 7, 2017

Language: Английский

Citations

19
Identification of potential long non-coding RNA biomarkers associated with the progression of colon cancer DOI Open Access
Jingwen Li,

Weinan Xue,

Junli Lv

et al.

Oncotarget, Journal Year: 2017, Volume and Issue: 8(44), P. 75834 - 75843

Published: May 17, 2017

// Jingwen Li 1, * , Weinan Xue Junli Lv 2, Peng Han 1 Yanlong Liu and Binbin Cui Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, 150081, China 2 Science, These authors contributed equally to this work Correspondence to: Cui, email: [email protected] Liu, [email protected] Keywords: biomarkers, colon cancer, disease progression, long non-coding RNA Received: March 29, 2017 Accepted: May 03, Published: 17, ABSTRACT Increasing evidence has suggested that dysregulated lncRNA expression played important roles in the development progression human cancers. Although prognostic lncRNAs have been recognized for cancer (CC) patients, search novel biomarkers potentially involved CC is an urgent still largely unmet medical need. In study, we evaluated changes during by analyzing two cohorts previously published profiles patients identified hundreds differentially expressed lncRNAs. Then eight closely associated with from a large number significantly altered using random forest supervised classification algorithm. Finally, SVM-based risk classifier was developed discriminate high-risk persons early-stage validated both training dataset testing survival analysis five-fold cross-validation strategy. Our pathway enrichment based on protein-coding genes are co-expressed lncRNAs, variation might affect critical pathways progression. With further validation, these significant implications clinical management early stage improve our understanding

Language: Английский

Citations

18