Cancer Metastasis‐on‐a‐Chip for Modeling Metastatic Cascade and Drug Screening

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(21)

Published: Jan. 15, 2024

Microfluidic chips are valuable tools for studying intricate cellular and cell-microenvironment interactions. Traditional in vitro cancer models lack accuracy mimicking the complexities of vivo tumor microenvironment. However, cancer-metastasis-on-a-chip (CMoC) combine advantages 3D cultures microfluidic technology, serving as powerful platforms exploring mechanisms facilitating drug screening. These able to compartmentalize metastatic cascade, deepening understanding its underlying mechanisms. This article provides an overview current CMoC models, focusing on distinctive that simulate invasion, intravasation, circulation, extravasation, colonization, their applications Furthermore, challenges faced by technologies discussed, while promising future directions research. The ongoing development integration these into studies expected drive transformative advancements field.

Language: Английский

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Angiogenesis within Atherosclerotic Plaques: Mechanical Regulation, Molecular Mechanism and Clinical Diagnosis

Mechanobiology in Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 100114 - 100114

Published: Feb. 1, 2025

Language: Английский

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Role of viscoelasticity in the appearance of low-Reynolds turbulence: considerations for modelling

Journal of Biological Engineering, Journal Year: 2024, Volume and Issue: 18(1)

Published: April 8, 2024

Inertial effects caused by perturbations of dynamical equilibrium during the flow soft matter constitute a hallmark turbulence. Such are attributable to an imbalance between energy storage and dissipation. During Newtonian fluids, kinetic can be both stored dissipated, while viscoelastic systems, such as polymer induces accumulation elastic energies. The causes local stiffening stretched chains, which destabilise flow. Migrating multicellular systems hugely complex capable self-regulating their viscoelasticity mechanical stress generation, well controlling Since perturbation is inhomogeneous energy, rather than turbulence occur at low Reynolds numbers.This theoretical review focused on clarifying role in appearance low-Reynolds Three types system considered compared: (1) high-Reynolds turbulent (2) moderate-Reynolds solutions, (3) migration epithelial collectives, discussed terms two model systems. models involve fusion aggregates, free expansion monolayers substrate matrix.

Language: Английский

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Tuneable hydrogel patterns in pillarless microfluidic devices

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(7), P. 2094 - 2106

Published: Jan. 1, 2024

Organ-on-chip (OOC) technology has recently emerged as a powerful tool to mimic physiological or pathophysiological conditions through cell culture in microfluidic devices. One of its main goals is bypassing animal testing and encouraging more personalized medicine. The recent incorporation hydrogels 3D scaffolds into devices changed biomedical research since they provide biomimetic extracellular matrix recreate tissue architectures. However, this presents some drawbacks such the necessity for physical structures pillars confine these hydrogels, well difficulty reaching different shapes patterns create convoluted gradients realistic biological structures. In addition, can also interfere with fluid flow, altering local shear forces and, therefore, modifying mechanical environment OOC model. work, we present methodology based on plasma surface treatment that allows building chambers abutment-free capable producing precise stress distributions. Therefore, pillarless arbitrary geometries are needed obtain versatile, reliable, experimental models. Through computational simulation studies, changes demonstrated designed fabricated geometries. To prove versatility new technique, blood-brain barrier model been recreated, achieving an uninterrupted endothelial emulates part neurovascular network brain. Finally, developed could avoid limitations mentioned above, allowing development models complex adaptable geometries, cell-to-cell contact if required, where flow be controlled.

Language: Английский

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Graphene Oxide (GO)-Based Bioink with Enhanced 3D Printability and Mechanical Properties for Tissue Engineering Applications

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(9), P. 760 - 760

Published: April 26, 2024

Currently, a major challenge in material engineering is to develop cell-safe biomaterial with significant utility processing technology such as 3D bioprinting. The main goal of this work was optimize the composition new graphene oxide (GO)-based bioink containing additional extracellular matrix (ECM) unique properties that may find application bioprinting biomimetic scaffolds. experimental evaluated functional viscosity and complex modulus, printability, mechanical strength, elasticity, degradation absorbability, well biological cytotoxicity cell response after exposure biomaterial. findings demonstrated inclusion GO had no substantial impact on rheological but it did enhance properties. This enhancement crucial for advancement scaffolds are resilient deformation promote their utilization tissue investigations. Furthermore, GO-based hydrogels exhibited much greater swelling, absorbability compared non-GO-based bioink. Additionally, these biomaterials showed lower cytotoxicity. Due its properties, recommended use models vascular system, e.g., testing drugs or hard models.

Language: Английский

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Triplicate Dynamic Cell Culture Platform for Enhanced Reproducibility in Anti-Cancer Drug Testing

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

The development of stable and standardized in vitro cytotoxicity testing models is essential for drug discovery personalized medicine. Microfluidic technologies, recognized their small size, reduced reagent consumption, control over experimental variables, have gained considerable attention. However, challenges associated with external pumps, particularly inconsistencies between individual pumping systems, limited the real-world application cancer-on-a-chip technology. This study introduces a novel triplicate cell culture system (Tri-CS) that simultaneously supports dynamic cultures three independent units using single peristaltic pump, ensuring consistent flow conditions. Our findings demonstrate Tri-CS significantly reduces variability compared to pump enhancing reliability anticancer testing. Furthermore, we evaluated gemcitabine cytotoxicity, which shows enhanced efficacy Fluorescein diffusion tests revealed greater efficiency cultures, contributed higher observed efficacy. potential broader Tri-CS, including its compatibility commercially available transwells opportunity use more complex cancer-on-chip models, positions this as valuable tool advancing microphysiological systems preclinical research.

Language: Английский

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Capacity and limitations of microfluidic flow to increase solute transport in three-dimensional cell cultures

Journal of The Royal Society Interface, Journal Year: 2025, Volume and Issue: 22(222)

Published: Jan. 1, 2025

Culturing living cells in three-dimensional environments increases the biological relevance of laboratory experiments, but requires solutes to overcome a diffusion barrier reach centre cellular constructs. We present theoretical and numerical investigation that brings mechanistic understanding how microfluidic culture conditions, including chamber size, inlet fluid velocity spatial confinement, affect solute distribution within Contact with substrate reduces maximally achievable construct radius by 15%. In practice, finite convection kinetics further lower limit. The benefits external are greater if transport rates across diffusion-dominated areas high. Those omnipresent include diffusive boundary layer growing from fluid–construct interface regions near corners where is recirculating. Such multiply required achieve given penetration up 100, so chip designs ought minimize them. Our results define conditions complete into an avascular cell applies real chambers without needing simulate their exact geometries.

Language: Английский

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The role of cellular senescence in endothelial dysfunction and vascular remodelling in arteriovenous fistula maturation

The Journal of Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Abstract Haemodialysis (HD) is often required for patients with end‐stage renal disease. Arteriovenous fistulas (AVFs), a surgical procedure connecting an artery to vein, are the preferred vascular access HD due their durability and lower complication rates. The aim of AVFs promote vein remodelling accommodate increased blood flow needed dialysis. However, many fail mature properly, making them unsuitable Successful maturation requires remodelling, resulting in luminal diameter thickened walls support flow. After AVF creation, haemodynamic changes on venous side initiate cascade events that, when successful, lead proper AVF, it suitable cannulation. In this process, endothelial cells play crucial role since they direct contact frictional forces exerted by blood, known as shear stress. Patients requiring have other conditions that increase burden senescent cells, such ageing, diabetes hypertension. These characterized irreversible growth arrest secretion pro‐inflammatory pro‐thrombotic factors, collectively senescence‐associated secretory phenotype (SASP). This accumulation can impair function promoting inflammation, reducing vasodilatation, increasing thrombosis risk, thus hindering function. review explores contribution potential therapeutic strategies alleviate effects cell accumulation, aiming improve image

Language: Английский

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Efficient Differentiation of hiPSCs into hMSC-like Cells under Chemically Defined Conditions on Temperature-Sensitive Micropatterned Surfaces

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: 17(9), P. 13358 - 13374

Published: Feb. 20, 2025

The fairness of long-term self-renewal and robust cell proliferation limits the applications human mesenchymal stem cells (hMSCs) in regenerative medicine. Inducing hMSCs from human-induced pluripotent (hiPSCs), which have advantages autogenous no number issues, is highly valuable. However, current induction methods using FBS-containing culture medium problems, including immunogenicity, microbial contamination, low efficiency. To solve these we propose a chemically defined protocol incorporating transforming growth factor-beta 1 retinoic acid (RA) additives serum-free E6 for suspension embryoid bodies hiPSCs. Additionally, microgroove-patterned polydimethylsiloxane surfaces coated with temperature-sensitive N-isopropylacrylamide (PNIPAAm) were prepared efficient harvesting purification induced hiPSC-derived (hiPSC-MSCs). results showed that both supplementation RA patterned microgrooves width 20 μm could accelerate hiPSC-MSCs, realizing promising scalable production homogeneous cells. This study successfully established utilized to obtain clinically applicable show great promise tissue engineering, gene therapy, transplantation.

Language: Английский

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From Adhesion to Biofilms Formation and Resilience: Exploring the Impact of Silver Nanoparticles-Based Biomaterials on Pseudomonas aeruginosa

Biofilm, Journal Year: 2025, Volume and Issue: 9, P. 100267 - 100267

Published: Feb. 28, 2025

Colonization of medical devices by microorganisms, often progressing to the formation resilient biofilms, presents a common clinical issue. To address this challenge, there is growing interest in developing novel biomaterials with antimicrobial/antibiofilm properties as promising preventive measure. This study explores nanocomposite based on silver nanoparticles (AgNPs) deposited thin silica (SiO2) layers for their potential effect adhesion, detachment, viability and biofilm opportunistic Pseudomonas aeruginosa. The AgNPs-based biointerface development investigated PAO1-Tn7-gfp strain combining experiments under static dynamic conditions. For latter, shear-stress flow chamber used mimic conditions encountered around certain devices. findings reveal rapid bactericidal AgNPs, noticeable within 30 min exposure. Moreover, delay surface colonization observed unstructured biofilm, even after 72h culture. A considerable fragility sensitivity hydrodynamic stresses noticed loosely attached bacterial monolayer when compared thick formed SiO2 surface. underlines conception surfaces controlled release biocidal agent.

Language: Английский

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