Ultrafast sound production mechanism in one of the smallest vertebrates

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(10)

Published: Feb. 26, 2024

Motion is the basis of nearly all animal behavior. Evolution has led to some extraordinary specializations propulsion mechanisms among invertebrates, including mandibles dracula ant and claw pistol shrimp. In contrast, vertebrate skeletal movement considered be limited by speed muscle, saturating around 250 Hz. Here, we describe unique mechanism which

Language: Английский

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Whole-brain optical access in a small adult vertebrate with two- and three-photon microscopy

iScience, Journal Year: 2022, Volume and Issue: 25(10), P. 105191 - 105191

Published: Sept. 23, 2022

Language: Английский

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Characterization of the vocal behavior of the miniature and transparent fish model, Danionella cerebrum

The Journal of the Acoustical Society of America, Journal Year: 2024, Volume and Issue: 155(1), P. 781 - 789

Published: Jan. 1, 2024

Danionella cerebrum has recently been proposed as a promising model to investigate the structure and function of adult vertebrate brain, including development vocal–auditory neural pathways. This genetically tractable transparent cypriniform is highly vocal, but limited information available on its acoustic behavior underlying biological function. Our main goal was characterize repertoire diel variation in sound production D. cerebrum, well relationship between vocal reproduction. Sound recordings demonstrated high activity, with sounds varying from short sequences pulses known “bursts” (comprising up 15 pulses) notably longer sounds, termed “long bursts”, which extended 349 over 2.7 s. Vocal activity peaked at midday it very low night only few bursts. While number higher during daytime, interpulse interval night. In addition, calling time positively associated viable eggs, suggesting that communication important for These preliminary findings reveal potential using plasticity implications sexual selection reproduction novel neuroscience.

Language: Английский

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Label‐Free Multiphoton Imaging Reveals Volumetric Shifts Across Development in Sensory‐Related Brain Regions of a Miniature Transparent Vertebrate

The Journal of Comparative Neurology, Journal Year: 2025, Volume and Issue: 533(4)

Published: April 1, 2025

ABSTRACT Animals integrate information from different sensory modalities as they mature and perform increasingly complex behaviors. This may parallel differential investment in specific brain regions depending on the changing demands of inputs. To investigate developmental changes volume canonical regions, we used third harmonic generation imaging for morphometric analysis forebrain midbrain larval through juvenile adult stages Danionella dracula , a transparent, miniature teleost fish whose is optically accessible throughout its lifespan. Relative to whole‐brain volume, increased or telencephalon, higher order integration center, shows most dramatic increases between 30–60 days postfertilization (dpf) again at 90 dpf animals reach adulthood. The torus longitudinalis (TL), visuomotor also significantly 60 dpf. In contrast, optic tectum (TeO), retinal‐recipient target, progressively decreases 30 dpf, whereas relatively consistent across all semicircularis (TS), secondary auditory mechanosensory lateral line olfactory bulb (OB), direct target epithelium. sum, higher‐order centers (telencephalon, TL) occurs juveniles adulthood (60–90 dpf) exhibit more cognitive tasks, modality‐dominant earlier (TeO) development (TS, OB). Complete optical access ’s lifespan provides unique opportunity how structure over correlates with connectivity, microcircuitry, behavior.

Language: Английский

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Label‐free, whole‐brain in vivo mapping in an adult vertebrate with third harmonic generation microscopy

The Journal of Comparative Neurology, Journal Year: 2024, Volume and Issue: 532(4)

Published: April 1, 2024

Abstract Comprehensive understanding of interconnected networks within the brain requires access to high resolution information large field views and over time. Currently, methods that enable mapping structural changes entire in vivo are extremely limited. Third harmonic generation (THG) can resolve myelinated structures, blood vessels, cell bodies throughout without need for any exogenous labeling. Together with deep penetration long wavelengths, this enables brain‐mapping fractions small animals Here, we demonstrate THG microscopy allows non‐invasive label‐free an adult vertebrate, Danionella dracula , which is a miniature species cyprinid fish. We show capability multiple regions particular identification major commissural fiber bundles midbrain hindbrain. These features provide readily discernable landmarks navigation regional‐specific neuronal groups even single neurons during experiments. further how technique easily be coupled fluorescence used as comparative tool studies other similar body such zebrafish ( Danio rerio) tetras Trochilocharax ornatus ). This new evidence, building on previous studies, demonstrates size relative transparency, combined unique capabilities microscopy, vertebrate brain.

Language: Английский

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Understanding collective behavior through neurobiology

Current Opinion in Neurobiology, Journal Year: 2024, Volume and Issue: 86, P. 102866 - 102866

Published: June 1, 2024

A variety of organisms exhibit collective movement, including schooling fish and flocking birds, where coordinated behavior emerges from the interactions between group members. Despite prevalence movement in nature, little is known about neural mechanisms producing each individual's within group. Here we discuss how a neurobiological approach can enrich our understanding by determining which individuals interact. We provide examples sensory systems for social communication during highlight recent discoveries detecting position actions partners, opportunities future research. Understanding neurobiology insight into nervous function dynamic world.

Language: Английский

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Establishment of the miniature fish speciesDanionella translucidaas a genetically and optically tractable neuroscience model

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2018, Volume and Issue: unknown

Published: Oct. 16, 2018

Summary Integrative studies of the diverse neuronal networks that govern social behavior are hindered by lack methods to record neural activity comprehensively across entire brain. The recent development miniature fish Danionella cerebrum as a model organism offers one potential solution, small size and optical transparency these animals makes it possible visualize circuit throughout nervous system 1–4 . Here, we establish feasibility using for socially reinforced learning showing adult exhibit strong affiliative tendencies, interactions can serve reinforcer in an appetitive conditioning paradigm. Fish exhibited acute ability identify conspecifics distinguish them from closely related species, which was mediated both visual particularly olfactory cues. These behaviors were abolished pharmacological genetic interference with oxytocin signaling, demonstrating conservation key mechanisms observed other vertebrates 5–11 Our work validates tool understanding brain general, its modulation neuropeptide signaling particular.

Language: Английский

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A Model of Discovery: The Role of Imaging Established and Emerging Non-mammalian Models in Neuroscience

Frontiers in Molecular Neuroscience, Journal Year: 2022, Volume and Issue: 15

Published: April 14, 2022

Rodents have been the dominant animal models in neurobiology and neurological disease research over past 60 years. The prevalent use of rats mice neuroscience has driven by several key attributes including their organ physiology being more similar to humans, availability a broad variety behavioral tests genetic tools, widely accessible reagents. However, despite many advances understanding that achieved using rodent models, there remain limitations questions can be addressed these other mammalian models. In particular, vivo imaging mammals at cell-resolution level remains technically difficult demands large investments time cost. simpler nervous systems non-mammalian allow for precise mapping circuits even whole brain with impressive subcellular resolution. types available spans vertebrates non-vertebrates, so an appropriate model most cell biological neurodegenerative likely exists. A push diversify used could help address current gaps knowledge, complement existing rodent-based bodies work, bring new insight into our human disease. Moreover, are inherent aspects such as lifespan tissue transparency make them specifically advantageous studies. Crispr/Cas9 gene editing decreased cost genome sequencing combined optical microscopy enhances utility specific questions. This review seeks synthesize knowledge established emerging organisms cellular-resolution techniques suggest approaches understand neurodegeneration neurobiological processes. We will summarize tools single scale lead increased consideration research.

Language: Английский

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The genome sequence of the Dracula fish, Danionella dracula (Britz, Conway & Rüber, 2009)

Wellcome Open Research, Journal Year: 2024, Volume and Issue: 9, P. 194 - 194

Published: April 12, 2024

We present a genome assembly from an individual Danionella dracula (the Dracula fish; Chordata; Actinopterygii; Cypriniformes; Danionidae; Danioninae). The sequence is 665.21 megabases in span. This scaffold-level assembly, with scaffold N50 of 10.29 Mb.

Language: Английский

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Oxytocin-mediated social preference and socially reinforced reward learning in the miniature fish Danionella cerebrum

Current Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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