Motile sperm subpopulations in bull semen using different clustering approaches – Associations with flow cytometric sperm characteristics and fertility

Animal Reproduction Science, Journal Year: 2020, Volume and Issue: 215, P. 106329 - 106329

Published: Feb. 21, 2020

Language: Английский

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Effect of frame rate capture frequency on sperm kinematic parameters and subpopulation structure definition in boars, analysed with a CASA‐Mot system

Reproduction in Domestic Animals, Journal Year: 2018, Volume and Issue: 54(2), P. 167 - 175

Published: Sept. 1, 2018

Contents Motility is the most widely used indicator of sperm quality. Computer‐Assisted Semen Analysis ( CASA ) allows objective evaluation motility parameters. technology a common tool to predict semen doses in farm animal reproduction. The kinds video cameras until now for image acquisition have presented limited frame rates FR ), which negative influence on quality obtained data. aim present work was define optimal rate correct boar and its subpopulation structure. Eighteen ejaculates from nine mature boars Pietrain breed were used. Using ISAS ® v1 ‐Mot system, with camera working up 200 Hz, six s (25, 50, 75, 100, 150 fps) compared. D4C20 counting chambers, warmed 37°C, affected all kinematic parameters, curvilinear velocity VCL BCF sensitive ones. All parameters showed differences among animals. Non‐linear correlation asymptotic level at 212 fps, being highest For future studies based just progressive motility, almost 100 fps 0.5 must be used, while when kinematics considered, one‐second should analysed. Three principal components (velocity, progressivity oscillation), similar 50 fps. Cells grouped four subpopulations but different cellular distribution both s.

Language: Английский

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Boar Sperm Motility Assessment Using Computer-Assisted Sperm Analysis: Current Practices, Limitations, and Methodological Challenges

Animals, Journal Year: 2025, Volume and Issue: 15(3), P. 305 - 305

Published: Jan. 22, 2025

Spermatozoa are highly specialized male cells that characterized by a unique ability to move, which is critical factor for successful fertilization. The relative simplicity of motility assessment, especially in livestock, has made it widely used parameter evaluating ejaculate quality or cryopreserved semen the clinical field, and an advanced tool reproductive physiology toxicology research. Technological advances image analysis computational methods have substantially increased its accuracy through use computer-assisted sperm (CASA) minimize subjective bias assessments. Nevertheless, this more objective method still presents some significant challenges, including variability sample preparation, imaging conditions, analytical parameters. These issues contribute inconsistency impair reproducibility comparability data between laboratories. implementation standardized protocols, combined with comprehensive training rigorous evaluation, can serve mitigate emerging inconsistencies. In addition, vitro conditions under CASA analyses performed often differ significantly from natural environment female tract vivo. This review discusses methodologies, issues, limitations using CASA, particular focus on boar as important agricultural biomedical model species system used.

Language: Английский

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Kinematic Sub-Populations in Bull Spermatozoa: A Comparison of Classical and Bayesian Approaches

Biology, Journal Year: 2020, Volume and Issue: 9(6), P. 138 - 138

Published: June 26, 2020

The ejaculate is heterogenous and sperm sub-populations with different kinematic patterns can be identified in various species. Nevertheless, although these are statistically well defined, the statistical differences not always relevant. aim of present study was to characterize from two bovine species, diluted commercial extenders, determine relevance through Bayesian analysis. Semen 10 bulls evaluated after thawing. An ISAS®v1 computer-assisted analysis (CASA)-Mot system employed an image acquisition rate 50 Hz ISAS®D4C20 counting chambers. Sub-populations motile spermatozoa were characterized using multivariate procedures such as principal components (PCs) clustering methods (k-means model). Four three PCs that involved progressiveness, velocity, cell undulatory movement. proportions varied extender used Despite a difference (p < 0.05) between confirmed only one them (Triladyl®) presented relevant when compared Tris-EY OptiXcell®. Extenders differed proportion cells each sub-populations. Similar Bos taurus indicus. results indicate SP1, SP2, SP3 for PC criteria For linearity, SP4 did show regarding other classical approach or subpopulation thus may have direct biological meaning. Therefore, needs reevaluated.

Language: Английский

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Modulatory effect of MG-132 proteasomal inhibition on boar sperm motility during in vitro capacitation

Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10

Published: March 23, 2023

A series of biochemical and biophysical changes during sperm capacitation initiates various signaling pathways related to protein phosphorylation leading hyperactivation, simultaneously with the regulation proteasomal activity responsible for degradation turnover. Our study aimed unveil role proteasome in boar motility, hyperactivated status, tyrosine phosphorylation, total ubiquitination. The proteolytic 20S core was inhibited by MG-132 concentrations 10, 25, 50, 100 μM; monitored parameters were analyzed every hour 3 h vitro (IVC). Sperm motility kinematic Computer Assisted Analysis (CASA) IVC, showing a significant, negative, dose-dependent effect on progressive (TMOT, PMOT, respectively). Furthermore, inhibition 50 μM had negative impact velocity-based (VSL, VAP, VCL). Parameters progressivity movement (LIN, STR) ALH most affected highest inhibitor concentration (100 μM). Cluster analysis revealed that strongest proteasome-inhibiting treatment significant (p ≤ 0.05) subpopulation. flow cytometric viability results proved reduced TMOT PMOT not caused disruption integrity plasma membrane. Neither profile nor accumulation ubiquitination observed course under inhibition. In conclusion, ability spermatozoa undergo hyperactivation; however, there no level ubiquitinated proteins. These effects might be due presence compensatory mechanisms or alteration ubiquitin-proteasome system-regulated pathways.

Language: Английский

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The application of computer-assisted semen analysis (CASA) technology to optimise semen evaluation. A review

Journal of Animal and Feed Sciences, Journal Year: 2020, Volume and Issue: 29(3), P. 189 - 198

Published: Sept. 21, 2020

1. Acosta A.A., Kruger T.F. (Editors), 1996. Human Spermatozoa in Assisted Reproduction. Parthenon Publishing Group. New York, NY (USA). Google Scholar

Language: Английский

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Sperm kinematics and morphometric subpopulations analysis with CASA systems: a review

Revista de Biología Tropical, Journal Year: 2019, Volume and Issue: 67(6)

Published: Nov. 6, 2019

Sperm kinematics and morphometric subpopulations analysis with CASA systems: a review. The subjective evaluation of seminal quality has given way to the use objective assessment techniques by technology (computer-assisted semen analysis). application principal components (PC) clustering methods reveal spermatozoa is powerful tool evaluate raw processed cell suspensions, but not many researchers are aware technique. PC multivariate statistical method that reduces number variables used in subsequent calculations describe data. By integrating original according their coherence database into new complex mathematical variable, clearly defined homogenous can be defined. Kinematic, morphometric, morphological or DNA integrity tests may apply characterize it understand reproductive biology spermatozoon. In recent years there been substantive change conceptual paradigm regarding what an ejaculate is. It should said until recently was considered representative population (billions) sperm made up "equivalent" cells common goal: one could finally fertilize oocyte. verification set grouped distinct subpopulations, kinematic characteristics, opens towards more cooperative vision. Besides, we have seen subpopulation distribution different among males, which seems indicate strategies understood within another paradigm, competition between ejaculates. existence heterogeneous show patterns widely known biological meaning these still clear. Although role remains unknown, work continue direction.

Language: Английский

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Sperm subpopulations influence the pregnancy rates in cattle

Reproduction in Domestic Animals, Journal Year: 2021, Volume and Issue: 56(8), P. 1117 - 1127

Published: May 20, 2021

This study aimed to the characteristics and subpopulations of spermatozoa from bulls with low high reproductive performance based on pregnancy rates. Based historical records rate four farms, 24 were selected. Two groups established, rates (n = 12; LOW), including that presented <52.27% (33.33% 51.81%); a group HIGH), >52.27% (52.27% 69.64%), after fixed-time artificial insemination (FTAI). The thawed sperm straws analysed kinetics, morphology, plasma membrane integrity subpopulations. LOW exhibited higher proportion static cells (p < .05). In contrast, HIGH showed greater percentages for total progressive motility, fast medium velocity cluster procedures, established. low-fertility highest percentage subpopulation 2 (41.46%), characterized by slow spermatozoa. high-fertility 3 (37.17%), nonlinear Results this indicated seem have fertilization capacity analysis can be considered tool identify ejaculates fertility.

Language: Английский

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Making the most of sperm activation responses: experiments with boar spermatozoa and bicarbonate

Reproduction Fertility and Development, Journal Year: 2018, Volume and Issue: 30(6), P. 842 - 842

Published: Jan. 1, 2018

Attempting to extract useful and reliable information about semen quality its fertility potential remains a difficult exercise, partly because the sperm heterogeneity within samples often renders simple statistical analyses rather meaningless. In fact, mean standard deviation may reflect neither very fast swimming activities of most active cells nor slow sluggish others. Herein we propose that value can be maximised if current knowledge activation mechanisms is exploited before undertaking measurements. We explain, using boar as an example, estimating defining relative subpopulation sizes, after by bicarbonate, provides means quantifying quality. Although such estimates indeed related in vivo fertility, general approach also suggests new avenues could for elaboration novel vitro tests characterisation toxic environmental chemicals and, indeed, reduce number animals used testing programs.

Language: Английский

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Cluster analysis to evaluate disease risk in periparturient dairy cattle

Animal Science Journal, Journal Year: 2020, Volume and Issue: 91(1)

Published: Jan. 1, 2020

Predicting periparturient disease risk is of immense value to the dairy industry. Periparturient diseases are interrelated with each other; however, predicting onset these has predominantly been based on a single blood parameter for disease. This study examined new diagnostic method predict diseases. We conducted cluster analysis multiple constituents from 20 Holstein cattle at 1 week post-partum, and were divided into two groups, A or B. then compared early-lactation groups. Group B had significantly higher 3-hydroxybutyric acid concentrations suspected have subclinical ketosis. also lower calcium concentrations, tendency hypocalcemia. performed discriminant using parameters which grouped population same groups as three variables: inorganic phosphorus, calcium, either phospholipids total cholesterol. further showed that functions could be used even before parturition. Our results indicate useful risks.

Language: Английский

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