Appraisal of ATP1B1 and GSTM3 proteins as freezability factors in buffalo ejaculated spermatozoa DOI Open Access
Marvin Bryan Segundo Salinas,

Thuchadaporn Chaikhun‐Marcou,

Phongsakorn Chuammitri

et al.

The Thai Journal of Veterinary Medicine, Journal Year: 2022, Volume and Issue: 52(4), P. 693 - 646

Published: Dec. 1, 2022

Sperm cryopreservation intensifies the use of assisted reproductive technologies in food animals. However, variations freezability confront frozen-thawed semen applications. Hence, this groundwork aimed to identify fundamental differences between high (HF) and low (LF) ejaculated spermatozoa swamp buffaloes, which were limitedly described for agricultural species. Twelve (12) different samples bubaline collected, cryopreserved categorized into phenotypes using post-thaw sperm motility as basis. kinematics functional plasma membrane integrity evaluated HF LF computer-assisted analysis (CASA) hypo-osmotic swelling test, respectively. The relative concentrations some selected proteins namely ATPase subunit beta 1 (ATP1B1) glutathione S-transferase Mu 3 (GSTM3) also comparatively quantified dot blot image processing analyses. results revealed that all CASA parameters except wobble straightness significantly higher than cluster (P<0.05). Moreover, exhibited more intact membranes under a state group there was no significant difference abundance ATP1B1 GSTM3 among phenotypes, suggesting other related energy production oxidative stress protection influence cryoresilience. Nevertheless, these findings reaffirmed existence variabilities outcomes buffaloes established key terms motility, integrity.

Language: Английский

The Protective Role of Melatonin in Sperm Cryopreservation of Farm Animals and Human: Lessons for Male Fish Cryopreservation DOI Creative Commons

Alexandra I. Alevra,

Athanasios Exadactylos, Eleni Mente

et al.

Animals, Journal Year: 2022, Volume and Issue: 12(6), P. 791 - 791

Published: March 21, 2022

Cryopreservation is a technique that offers various advantages, especially in fish, among others, makes the reproduction of species easier through constant supply sperm, synchronization gamete availability both sexes, storage semen for genetic improvement programs, reduction cost by eliminating need to maintain male broodstock, and conserving gametes endangered species. However, freezing warming procedures cryopreservation lead quality viability cryopreserved sperm because oxidative stress. For this reason, enrichment extender media with antioxidants common method several fish Recently, many studies have been published protective role melatonin on fertility preservation farm animals humans, demonstrating beneficial effects as cryoprotectant. On other hand, very few were conducted using an antioxidant different cryopreservation. We conclude use moderate concentrations are preservation, mechanisms which acts positively spermatozoa be further investigated establish protocols

Language: Английский

Citations

26
Oxidative stress and DNA fragmentation in frozen/thawed common Carp Cyprinus carpio sperm with and without supplemental proteins DOI
Anna Shaliutina‐Kolešová, Dmitry S. Loginov

Animal Reproduction Science, Journal Year: 2023, Volume and Issue: 251, P. 107213 - 107213

Published: March 13, 2023

Language: Английский

Citations

11
Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications DOI Creative Commons

Qingyuan Cheng,

Liman Li,

Min Jiang

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(18), P. 2845 - 2845

Published: Sept. 12, 2022

Preservation of human spermatozoa in vitro at normothermia or hypothermia maintaining their functions and fertility for several days plays a significant role reproductive biology medicine. However, it is well known that left deteriorate over time irreversibly as the consequence various stresses such change osmolarity, energy deficiency, oxidative damage, leading to substantial limitations including need semen examinations, preservation, assisted technology. These problems may be addressed with aid non-freezing storage techniques. The main most effective preservation strategies are partial total replacement seminal plasma culture medium, named extenders, temperature-induced metabolic restriction. Semen extenders consist buffers, osmolytes, antioxidants, etc. protect against above-mentioned adverse factors. Extended has negative effect on sperm parameters, whereas its ART outcomes remains inconsistent. duration, temperature, pre-treatment should determined according aims preservation. Advanced techniques nanotechnology omics have been introduced show great potential lifespan extension sperm. It certain more patients will benefit from near future. This review provided an overview current knowledge prospects prolonged vitro.

Language: Английский

Citations

15
SOME FATTY ACID AND SEMEN CHARACTERISTICS OF HOLSTEIN BULLS AS INLUENCED BY DIFFERENT SPERM FREEZABALITY DOI Creative Commons
S. M. Eidan,

R. I. Khalil,

Amirjanati Naser

et al.

IRAQI JOURNAL OF AGRICULTURAL SCIENCES, Journal Year: 2024, Volume and Issue: 55(2), P. 675 - 682

Published: April 28, 2024

This study was conducted to investigate the relationship between concentration of some fatty acids in seminal plasma and semen characteristics Holstein bulls with different sperm freezabilities. The straws that cryopreserved for two years were divided into three groups according sperm’s freezability: medium (MF,73.02%), good (GF,83.76%), high (HF,91.20%). cell individual motility (SCIM) fresh superior (P≤0.05) MF GF as compared HF bulls. Concomitantly, significantly those total acid concentrations. Moreover, recorded lesser DNA damage percentage differences among concentrations SCIM at 48 hours post-cryopreservation lacked significance. In conclusion, greater bull's lowest freezability, which indicated more sensitivity freezing conditions may reflect fertilizing ability these

Language: Английский

Citations

2
Cryopreservation of goat spermatozoa in the Mekong Delta region of Vietnam: evaluating cryoprotectant effectiveness DOI Creative Commons

Thi Thanh Khuong Tran,

Lam Khanh Duy Nguyen,

Thi Kim Ngan Nguyen

et al.

Veterinary Integrative Sciences, Journal Year: 2024, Volume and Issue: 22(1), P. 131 - 140

Published: April 2, 2024

Conserving and improving the quality of breeds is crucial for enhancing small ruminant production in Vietnam. To support this purpose, cryopreservation was offered as a promising method semen preservation maintaining genetic diversity. Moreover, many small-scale farmers Mekong Delta rely on goats both income nutrition, making them an important part farming activities. This study aimed to develop sperm protocol identify optimal cryoprotectant goat region Semen samples were diluted with TCG-EggYolk freezing medium containing agent (CPA) 1:1 ratio. The then placed 0.5 mL French straws, cooled 15 °C 5 °C, subjected nitrogen vapor before being immersed liquid at -196 °C. Experiment 1 utilized Glycerol CPA concentrations 0 %, 8 11 %. 2 employed dimethyl sulfoxide (DMSO) same concentrations. Thawed evaluated quality. Results indicated that 1, increasing concentration improved overall motility, viability, membrane integrity sperm. best outcomes achieved % Glycerol, resulting progressive motility 57.5 (P<0.05). In 2, extender DMSO demonstrated significantly better performance terms compared or DMSO. Progressive 53.5 conclusion, determined using choice

Language: Английский

Citations

1
Current perspectives on ruminant sperm freezability: Harnessing molecular changes related to semen quality through omics technologies DOI Creative Commons
Marvin Bryan Segundo Salinas, Phongsakorn Chuammitri, Korawan Sringarm

et al.

Veterinary Integrative Sciences, Journal Year: 2021, Volume and Issue: 19(3), P. 487 - 511

Published: May 6, 2021

The recent advances in sperm cryopreservation transcend cryobanking and other assisted reproductive technologies. Since its discovery, has contributed positive impacts on animal breeding as well genetic exchange, improvement, conservation efforts. However, cryoinjury variabilities outcomes remain key challenges to cryobiology. present work explored the molecular bases for such freezability differences freezing-thawing injuries ruminant sperm. Relevant biomarkers identified seminal plasma spermatozoa were highlighted, including lipids, proteins, metabolites, transcripts, genes. Specific mechanisms concerning structures functions also examined relative their association cryotolerance, spermiogram or seminogram modifications following procedures. Current conflicts gaps knowledge base emphasized. Further investigation of these areas using available breakthrough tools omics technologies is therefore proposed improve, optimize, even predict overall quality frozen-thawed semen towards efficiency.

Language: Английский

Citations

8
Influence of Sperm Number and Antioxidant Melatonin in Extender on the Quality of Post-Thawing Sheep Spermatozoa DOI Open Access

Frilianty Putri,

Ni Wayan Kurniani Karja,

Mohamad Agus Setiadi

et al.

Jurnal Ilmu Ternak dan Veteriner, Journal Year: 2023, Volume and Issue: 28(1), P. 1 - 10

Published: Feb. 2, 2023

This study aimed to examine the effect of spermatozoa concentration and effectiveness melatonin supplementation in diluent on quality post-thawing semen. Ejaculated semen was collected using artificial vaginal method (MVB). The carried out two stages, firstly frozen andromed with different concentrations one straw (50, 100, 200 million per straw), second supplemented doses (0, 0,5, 1, 0 1.5 mM melatonin). Parameters observed were movement Computer Assisted Sperm Analysis, membrane integrity, acrosome integrity. Data analyzed ANOVA further tested Duncan's test. results showed no significant difference pattern sheep when at 50, or (P 0.05). Furthermore, adding this affected spermatozoa's total motility progressive a 1.0 (P0.05) but did not significantly affect percentage characteristics. From results, it can be concluded that does semen, reduce effects frozen-thawed process motility, cap, plasma integrity Melatonin 1 extender highest study.

Language: Английский

Citations

0
Appraisal of ATP1B1 and GSTM3 proteins as freezability factors in buffalo ejaculated spermatozoa DOI Open Access
Marvin Bryan Segundo Salinas,

Thuchadaporn Chaikhun‐Marcou,

Phongsakorn Chuammitri

et al.

The Thai Journal of Veterinary Medicine, Journal Year: 2022, Volume and Issue: 52(4), P. 693 - 646

Published: Dec. 1, 2022

Sperm cryopreservation intensifies the use of assisted reproductive technologies in food animals. However, variations freezability confront frozen-thawed semen applications. Hence, this groundwork aimed to identify fundamental differences between high (HF) and low (LF) ejaculated spermatozoa swamp buffaloes, which were limitedly described for agricultural species. Twelve (12) different samples bubaline collected, cryopreserved categorized into phenotypes using post-thaw sperm motility as basis. kinematics functional plasma membrane integrity evaluated HF LF computer-assisted analysis (CASA) hypo-osmotic swelling test, respectively. The relative concentrations some selected proteins namely ATPase subunit beta 1 (ATP1B1) glutathione S-transferase Mu 3 (GSTM3) also comparatively quantified dot blot image processing analyses. results revealed that all CASA parameters except wobble straightness significantly higher than cluster (P<0.05). Moreover, exhibited more intact membranes under a state group there was no significant difference abundance ATP1B1 GSTM3 among phenotypes, suggesting other related energy production oxidative stress protection influence cryoresilience. Nevertheless, these findings reaffirmed existence variabilities outcomes buffaloes established key terms motility, integrity.

Language: Английский

Citations

0