The potential significance of antioxidants in livestock reproduction: sperm viability and cryopreservation

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 267, P. 107512 - 107512

Published: May 24, 2024

Language: Английский

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Comparing the effect of addition of different supplements to sperm preparation media on intracytoplasmic injection outcome

The Journal of Basic and Applied Zoology, Journal Year: 2025, Volume and Issue: 86(1)

Published: Jan. 11, 2025

Abstract Background To assess the effectiveness of human sperm treated with an egg yolk-enhanced refrigeration group and to determine protective effect selenium supplementation in a laboratory setting, samples were prepared using density gradient method as standard preparation technique. Additionally, study aimed investigate how these treatments affected outcomes intracytoplasmic injection (ICSI). This randomized, double-blind, placebo-controlled clinical trial involved 263 couples seeking treatment for male infertility between December 2021 2023. Couples pursuing ICSI primary or secondary included study. The procedure evaluated across groups. Results No statistically significant differences observed terms number injected oocytes, fertilized fertilization rates. However, showed significantly higher top-cleaved oocytes greater top cleavage rate compared ( p = 0.02). had transferred embryos < 0.001). Similarly, demonstrated than 0.04 0.001, respectively). was also group. Conclusions Both yolk buffer positive effects on oocyte maturation embryo development, resulting rates increased embryos. pregnancy following each technique individually comparable those achieved method.

Language: Английский

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Exosomes from porcine serum as endogenous additive maintain function of boar sperm during liquid preservation at 17 °C in vitro

Theriogenology, Journal Year: 2024, Volume and Issue: 219, P. 147 - 156

Published: Feb. 16, 2024

Language: Английский

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In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(23), P. 14646 - 14646

Published: Nov. 24, 2022

Since the molecular similarities and differences among physiological capacitation cryocapacitation have not been studied in detail, this study was designed to assess gene protein expression levels of Cation channel sperm (CatSper) 1 2, sodium bicarbonate (Na+/HCO3−) cotransporter (NBC) kinase A (PKA) un-capacitated (control), vitro capacitated (CAP) cryopreserved (CRYO) bovine spermatozoa. All samples were subjected motility evaluation using computer assisted analysis chlortetracycline (CTC) assay for assessment patterns. Furthermore, quantitative reverse transcription PCR (qRT-PCR) Western blots used monitor patterns selected markers. The results showed a significant reduction CatSper1 2 CRYO group when compared CAP (p < 0.0001). In case NBC, significantly different or inconclusive. While non-significant down-regulation PKA found group, frozen-thawed spermatozoa comparison 0.05). conclusion, we may hypothesize that while exhibit CTC-patterns consistent with events, machinery underlying CTC-positivity be different.

Language: Английский

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Roles of Y-27632 on sheep sperm metabolism

Journal of Animal Science, Journal Year: 2024, Volume and Issue: 102

Published: Jan. 1, 2024

Abstract To investigate the effect of Y-27632 on low-temperature metabolism sheep sperm, different concentrations were added to semen at 4 °C in this experiment detect indicators such as sperm motility, plasma membrane, acrosome, antioxidant performance, mitochondrial membrane potential (MMP), and metabolomics. The results showed that addition 20 µM significantly increased integrity rate, acrosome capacity, MMP level, adenosine triphosphate (ATP) total cholesterol content, reduced Ca2+ content. In metabolomics analysis, compared with control group, group screened differential metabolites, mainly involved five metabolic pathways, most significant difference Histidine (P = 0.001). confirmed improved quality preservation under conditions.

Language: Английский

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Spermicidal and antioxidant potency of Solanum macrocarpon L. (African eggplant) leaf ethanol extract in albino rats

Comparative Clinical Pathology, Journal Year: 2024, Volume and Issue: 33(3), P. 367 - 377

Published: Feb. 3, 2024

Language: Английский

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Manipulation of metabolism to improve liquid preservation of mammalian spermatozoa

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 271, P. 107631 - 107631

Published: Nov. 2, 2024

Reproductive success in mammals hinges on the ability of sperm to generate sufficient energy through cellular metabolism perform energy-intensive processes required for fertilisation, including motility, maturation, and oocyte interactions. It is now widely accepted that exhibit metabolic flexibility, utilising a combination glycolysis oxidative phosphorylation (supported by Krebs cycle other complementary pathways) meet their demands. However, preferred pathway production varies significantly among species, making it challenging map species-specific strategies, particularly species with high like ram. Additionally, differences methodologies used measure have led biased interpretations species' complicating development liquid storage methods aimed at preserving spermatozoa manipulating generation based requirements. This review examines requirements, current assessing capacity, research metabolism. Future should focus establishing standardised approach determining preferences accurately critical step before developing effective preservation methods. By identifying regulatory points, strategies can be designed temporarily inhibit pathways, conserving resources reducing accumulation by-products. Alternatively, supplementation depleted metabolites guided understanding areas excessive consumption during prolonged Applying this knowledge develop tailored techniques will help minimise damage improve survival vitro processing storage, ultimately enhancing artificial breeding programs.

Language: Английский

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Comparative evaluation of Duragen® and skim milk extenders to enhance ram semen quality and fertility: A promising alternative in ovine artificial insemination

Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: 58(8), P. 1132 - 1138

Published: June 22, 2023

This study aimed to investigate the effectiveness of Duragen® and skimmed milk (SM) extenders on quality parameters, bacterial load fertilization ability stored ram semen. A total 50 ejaculates from Sardi rams (n = 5) aged 2.5-3 years, were collected in SM at 15°C. The motilities velocity parameters generated by CASA system then evaluated 0, 8 24 h storage. Afterward, loads sperm extended determined 5 incubation. In addition, ewes 100) 2 have been chosen same herd. selected synchronized inseminated using semen for results revealed that progressive motilities, straight (VSL), straightness (SRT), lateral head displacement (ALH) beat cross frequency (BCF) not affected extender type after storage (p > .05). However, curvilinear (VCL), average path (VAP), linearity (LIN) wobble (WOB) showed higher values compared with < Bacterial observed mainly (183 UFC/mL) (357 only case showing a is when time attains (199 UFC/mL). Concerning fertility, diluted both resulting high fertility rates which reaches 66% 73% SM, respectively, no statistical difference summary, decreased maintained fertility. These findings suggest could be used as alternative ovine artificial insemination (OAI).

Language: Английский

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Enzymatic tissue processing after testicular biopsy in non-obstructive azoospermia enhances sperm retrieval

Human Reproduction Open, Journal Year: 2023, Volume and Issue: 2023(4)

Published: Jan. 1, 2023

Abstract STUDY QUESTION What is the added value of enzymatic processing testicular biopsies on sperm retrieval (SR) rates for patients with non-obstructive azoospermia (NOA)? SUMMARY ANSWER In addition to mechanical mincing, digestion increased SR in NOA patients. WHAT IS KNOWN ALREADY Many studies focus surgical approach optimize recovery NOA, and spite that, controversy still exists about whether type surgery makes any difference as long multiple are taken. Few studies, however, role IVF laboratory benefit additional lab procedures, e.g. digestion, order rates. DESIGN, SIZE, DURATION This retrospective single-center cohort study included all who underwent their first extraction (TESE) by open multiple-biopsy method between January 2004 July 2022. Only a normal karyotype, absence Y-q deletions diagnosis based histology were included. The primary outcome was rate after mincing and/or enzymes. secondary cumulative live birth (CLB) ICSI fresh TESE subsequent cycles frozen TESE. PARTICIPANTS/MATERIALS, SETTING, METHODS Multiple obtained from testis, unilaterally or bilaterally, day oocyte retrieval. Upon investigated 30 min; when no insufficient numbers spermatozoa observed, treatment performed using Collagenase IV. Multivariable regression analysis predict CLB per adjusting following confounding factors: male FSH level, female age, requirement find sperm. MAIN RESULTS AND THE ROLE OF CHANCE We 118 patients, whom 72 (61.0%) had successful eventually. Spermatozoa retrieved 28 (23.7%; 28/118) another 44 (37.2%; 44/118). Thus, 90 requiring (48.9%). Male characteristics not different regard mean age (34.5 vs 34.5 years), testis volume (10.2 10.6 ml), (17.8 16.9 IU/l), cryptorchidism (21.4 34.1%), varicocele (3.6 4.6%), histological (Sertoli-cell only 53.6 47.7%, maturation arrest 21.4 38.6%, sclerosis/atrophy 25.0 13.6%). Of available ICSI, 23/72 (31.9%) achieved (LB) injection (and embryo transfers). remaining 49 without LB, 34 (69.4%) supernumerary frozen. these 19 (55.9%) continued sperm, 9/19 (47.4%) an LB total 32/118 (27.1%) 32/72 (44.4%) retrieved. (couples available), (30.3 32.7 years; P = 0.042) significantly lower group compared those without. 31.8% (14/44), while alone 64.3% (18/28). logistic showed that required, it associated significant decrease (OR: 0.23 (0.08–0.7); 0.01). LIMITATIONS, REASONS FOR CAUTION Limitations related design. However, selection specific (normal karyotype deletion) having TESE, strengthens our findings. WIDER IMPLICATIONS FINDINGS Enzymatic increases only, demonstrating importance appropriate protocol. should be counseled have been found chances father genetically own child may digestion. FUNDING/COMPETING INTEREST(S) None reported. TRIAL REGISTRATION NUMBER N/A.

Language: Английский

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Carboxyfullerene C60 preserves sperm by enhancing antioxidant capacity and inhibiting apoptosis and harmful bacteria

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 6, 2024

Abstract Background The current approaches for the preservation of human sperm have several limitations, and there are a lack effective non-freezing methods. Recently, carboxyfullerene C 60 (CF-C ) has gained attention as an important nanocarbon derivative with strong antioxidant antibacterial activity. This study uses porcine model to systematically investigate whether CF-C can be used sperm. Results results indicate that supplementation preserve quality during storage at 17°C. effect is attributable improvement in capacity through decrease ROS level. Additionally, maintain mitochondrial function, inhibit apoptosis ROS/Cytochrome C/Caspase 3 signaling pathway, mediate suppression bacterial growth effects ROS. Finally, artificial insemination experiments -treated increase total number offspring born reduce deformed piglets. Conclusions Thus, by inhibition via reduction levels safe use component semen diluent storage. These findings pave way prospective clinical application carbon nano-materials antioxidants

Language: Английский

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