Evaluation of primers targeting chromo helicase DNA-binding gene (CHD) for molecular sexing identification in four bird families

Livestock and Animal Research, Journal Year: 2023, Volume and Issue: 21(1), P. 14 - 14

Published: March 5, 2023

<p><span class="fontstyle0">Objective: </span></p><p><span class="fontstyle2">Sex determination of birds is crucial role in breeding and conservation purposes. Genomic approaches by using basis bird sex polymorphism are accepted to generate an accurate sexing procedure. The PCR method commonly used known provide test results determining the birds, but its successes strongly influenced compatibility primers with DNA template. This study aimed determine potential use CHD1LF/CHD1LR targeting CHD gene fragment 4 species from Phasianidae, Psittacidae, Estrildidae, Passeridae families.</span></p><p><span class="fontstyle2"><br /></span><span class="fontstyle0">Method: class="fontstyle2">Blood samples (n= 30) representatives families above were collected for isolation, then was performed primers. obtained compared positive controls, necropsy examination information sellers.</span></p><p><span class="fontstyle0">Result: class="fontstyle2">The showed that primer could be Phasianidae,<br />Psittacidae, families. product confirmed same as well control results. It demonstrated male a single band (474 bp), while double bands 319 bp) observed female birds. Different accuracies during this between molecular approach manual seller i.e:<br />sparrows (46%) finches (50%), accuracy parakeets 75%, quails 80%.</span></p><p><span class="fontstyle0">Conclusions: class="fontstyle2">Primer can families<br />Phasianidae, Passeridae. Our molecular-based valuable able reduce error rate 100% procedures.</span> <br /><br /></p><p> </p>

Language: Английский

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Identification of sex‐linked SNP markers in wild populations of monomorphic birds

Molecular Ecology Resources, Journal Year: 2023, Volume and Issue: 23(8), P. 1905 - 1913

Published: Sept. 7, 2023

Abstract Single‐nucleotide polymorphism (SNP) analysis is a powerful tool for population genetics, pedigree reconstruction and phenotypic trait mapping. However, the untapped potential of SNP markers to discriminate sex individuals in species with reduced sexual dimorphism or during immature stages remains largely unexplored avenue. Here, we developed novel protocol molecular sexing birds based on detection unique Z‐ W‐linked markers. Our method identification two loci, one each chromosome. Individuals are considered males when they show no calls heterozygous homozygous Z‐linked SNP, while females exhibit both calls. We validated Jackdaw ( Corvus monedula ). The this makes it difficult identify wild. assessed reliability using 36 known found that their was correctly assigned 100% cases. sex‐linked also proved be widely applicable discriminating from sample 927 genotyped at different maturity stages, an accuracy 99.5%. Since increasingly used quantitative genetic analyses wild populations, approach propose has great integrated into broader research programmes without need additional techniques.

Language: Английский

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Mechanical problem solving by plush-crested jays: are tools special after all?

Animal Cognition, Journal Year: 2024, Volume and Issue: 27(1)

Published: Dec. 6, 2024

Tool use is taxonomically associated with high behavioural flexibility and innovativeness, its prevalence greater in primates some bird species. This association, however, not known to be causally determinant of tool-related competence since innovativeness are often observed the absence tool vice versa. For this reason, it interesting explore whether animals that can loosely categorized as outstanding, or 'intelligent' physical problem solvers, also remarkable using tools innovatively, rather than presenting special constraints. We investigate plush-crested jays (Cyanocorax chrysops), a corvid new cognitive research shows highly flexible inquisitive behaviour wild has been reported tools. tested two tasks apparent similar manipulative complexity incentive, one involving (T) other (NT). In NT task birds had open box transparent lid blocked by latch get reward, whereas T task, they rake pull out reward from box. Eight nine subjects succeeded none them learned solve task. consistent dedicated competencies, just problem-solving proficiency.

Language: Английский

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Identification of sex-linked SNP markers in wild populations of monomorphic birds

Authorea (Authorea), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 2, 2023

Single-nucleotide polymorphism (SNP) analyses are a powerful tool for population genetics, pedigree reconstruction and phenotypic trait mapping. SNPs could also be useful sexing individuals in species with reduced sexual dimorphism, yet this possibility remains poorly explored. Here, we develop novel protocol molecular of birds based on the detection unique Z- W-linked SNP markers. Our method is identification two loci, one each chromosome. Individuals considered males when they heterozygotic Z-linked females homozygote have SNP. We validated Jackdaw (Corvus monedula), whose dimorphism makes it difficult to sex wild. assessed reliability 36 known sex, found that their was correctly assigned 100% cases. The sex-linked markers proved widely applicable discriminate from sample 927 genotyped different maturity stages an accuracy 99.5%. Given increasingly used quantitative genetic wild populations, approach propose has great potential integrated into broader research programmes without need additional techniques.

Language: Английский

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Variation in nest‐site occupancy and breeding outcome in a threatened Subantarctic raptor

Ibis, Journal Year: 2023, Volume and Issue: 166(2), P. 504 - 517

Published: May 15, 2023

Breeding outcome in birds can be influenced by both intrinsic and extrinsic factors, which may contribute differentially to each stage of the breeding cycle. Island raptors are relatively threatened species that rely on simplified food webs usually co‐occur with invasive species. Here, we studied a population Striated Caracara Phalcoboenus australis Isla de los Estados (Argentina) understand relative contribution availability, topographical features territoriality parameters. Caracaras area depend short seasonal (i.e. pulsed) availability seabirds for feeding their chicks. On other hand, recent introduction non‐native ungulates have led suboptimal environment reducing nest‐site within seabird colonies via disturbance grassland. Using data from seven seasons analyse abundance, proportion individuals, spatial structure events population‐level performance, including chick sex ratio, study whether ecology this is still shaped marine web pulses or could associated herbivore disturbance. The adults represented only 6–15% population. pattern nest‐sites did not differ random arrangement after considering heterogeneous distribution availability. Territory identity accounted most variation clutch size productivity, penguin explained part variability territory occupancy hatching rate. ratio was biased towards females varied according brood size, more productive territories producing 91% males. cycle mainly dependent upon Rockhopper Penguin Eudyptes chrysocome pulse presents mixed evidence being disrupted herbivores. factors contributed output reproductive stage, accounting variation. Our results suggest individual‐level, long‐term relevant understanding dynamics Fuegian archipelago, thus broad‐scale research programme warranted assess conservation status propose future management scenarios

Language: Английский

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Comparison of three primer pairs for molecular sex determination in Eurasian pygmy owls (Glaucidium passerinum)

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 16, 2024

Bird sex determination is fundamental in various ecological and biological studies, although many avian species cannot be sexed visually due to their monomorphic and/or monochromatic appearance. Thus, reliable laboratory methods for sexing are a prerequisite. Most nestlings lack sex-related signs, including the Eurasian pygmy owl (Glaucidium passerinum). We performed analysis of this using blood samples 242 juveniles nine adults. It relied on qPCR specific intron from chromo-helicase DNA-binding protein 1 gene. tested three primer sets, P2/P8, 2550F/2718R, CHD1F/CHD1R, commonly used bird sexing. The outcomes were displayed an agarose gel electrophoresis plot melt curve analysis, which had not been previously conducted owls. found that only set CHD1F/CHD1R proved reliable, as one determined with two band/s peak/s males females, respectively. other pairs failed depicted band/peak all specimens regardless sex. Therefore, we recommend performing owls' by primers only.

Language: Английский

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