Molecular and circuit mechanisms mediating circadian clock output in the Drosophila brain

European Journal of Neuroscience, Journal Year: 2018, Volume and Issue: 51(1), P. 268 - 281

Published: July 30, 2018

Abstract A central question in the circadian biology field concerns mechanisms that translate ~24‐hr oscillations of molecular clock into overt rhythms. Drosophila melanogaster is a powerful system provided first understanding how clocks are generated and now illuminating neural basis behavior. The identity ~150 neurons brain their roles shaping rhythms locomotor activity have been described before. This review summarizes transmit time‐of‐day signals from clock, within network as well downstream it. We also discuss identification functional multisynaptic circuits between output regulate activity.

Language: Английский

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Membrane Currents, Gene Expression, and Circadian Clocks

Cold Spring Harbor Perspectives in Biology, Journal Year: 2017, Volume and Issue: 9(5), P. a027714 - a027714

Published: Feb. 28, 2017

Charles N. Allen1, Michael Nitabach2 and Christopher S. Colwell3 1Oregon Institute of Occupational Health Sciences Department Behavioral Neuroscience, Oregon & Science University, Portland, 97239 2Department Cellular Molecular Physiology Genetics, Yale School Medicine, New Haven, Connecticut 06520 3Department Psychiatry Biobehavioral Sciences, University California, Los Angeles, California 90024 Correspondence: michael.nitabach{at}yale.edu

Language: Английский

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Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Nov. 8, 2019

Circadian rhythms are generated by the cyclic transcription, translation, and degradation of clock gene products, including timeless (tim), but how circadian senses adapts to temperature changes is not completely understood. Here, we show that dramatically splicing pattern tim in Drosophila. We found at 18°C, TIM levels low because induction two cold-specific isoforms: tim-cold tim-short cold. At 29°C, another isoform, tim-medium, upregulated. Isoform switching regulates activity as each isoform has a specific function. cold encodes protein rescues behavioral defects tim01 mutants, flies which abrogated have abnormal locomotor activity. In addition, miRNA-mediated control limits expression some these isoforms. Finally, data obtained using minigenes suggest alternative might act thermometer for clock.

Language: Английский

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Circadian light-input pathways inDrosophila

Communicative & Integrative Biology, Journal Year: 2015, Volume and Issue: 9(1), P. e1102805 - e1102805

Published: Dec. 4, 2015

Light is the most important environmental cue to entrain circadian clock in animals. In fruit fly Drosophila melanogaster, light entrainment mechanisms of have been well-studied. The brain contains approximately 150 neurons that rhythmically express genes. These are called "clock neurons" and control behavioral activity rhythms. Many Cryptochrome (CRY) protein, which sensitive UV blue light, thus enables deep directly perceive light. addition CRY external photoreceptors eyes play an role light-input pathways. Recent studies provided new insights into integrate these inputs network brain. this review, we will summarize current knowledge on pathways clock.

Language: Английский

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Regulation of sleep plasticity by a thermo-sensitive circuit in Drosophila

Scientific Reports, Journal Year: 2017, Volume and Issue: 7(1)

Published: Jan. 13, 2017

Sleep is a highly conserved and essential behaviour in many species, including the fruit fly Drosophila melanogaster. In wild, sensory signalling encoding environmental information must be integrated with sleep drive to ensure that not initiated during detrimental conditions. However, molecular circuit mechanisms by which timing modulated environment are unclear. Here we introduce novel behavioural paradigm study this issue. We show male flies, onset of daytime siesta delayed ambient temperatures above 29 °C. term effect Prolonged Morning Wakefulness (PMW). through TrpA1 thermo-sensor required for PMW, specifically impacts onset, but night response elevated temperatures. identify two critical TrpA1-expressing circuits both contact DN1p clock neurons, output also PMW. Finally, circadian blue-light photoreceptor CRYPTOCHROME as regulator propose model nervous system integrates temperature, light, time dynamically control when initiated. Our results provide platform investigate how inputs co-ordinately regulate plasticity.

Language: Английский

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