Complex yeast–bacteria interactions affect the yield of industrial ethanol fermentation

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: March 8, 2021

Abstract Sugarcane ethanol fermentation represents a simple microbial community dominated by S. cerevisiae and co-occurring bacteria with clearly defined functionality. In this study, we dissect the interactions in sugarcane combinatorically reconstituting every possible combination of species, comprising approximately 80% biodiversity terms relative abundance. Functional landscape analysis shows that higher-order counterbalance negative effect pairwise on yield. addition, find Lactobacillus amylovorus improves yeast growth rate yield cross-feeding acetaldehyde, as shown flux balance laboratory experiments. Our results suggest could be considered beneficial bacterium potential to improve yields almost 3%. These data highlight biotechnological importance comprehensively studying communities extended other systems relevance human health environment.

Language: Английский

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Dynamic metabolic adaptation can promote species coexistence in competitive microbial communities

PLoS Computational Biology, Journal Year: 2020, Volume and Issue: 16(5), P. e1007896 - e1007896

Published: May 7, 2020

Microbes are capable of physiologically adapting to diverse environmental conditions by differentially varying the rates at which they uptake different nutrients. In particular, microbes can switch hierarchically between energy sources, consuming first those that ensure highest growth rate. Experimentally, this result in biphasic curves called "diauxic shifts" typically arise when grown media containing several Despite these observations well known microbiology and molecular biology, mathematical models generally used describe population dynamics microbial communities do not account for dynamic metabolic adaptation, thus implicitly assuming cannot dynamically from one resource another. Here, we introduce adaptation framework consumer-resource models, commonly competitive communities, allowing each species temporally change its preferred source maximize own relative fitness. We show enables community self-organize, coexist even presence few resources, respond optimally a time-dependent environment, showing could be an important mechanism maintaining high levels diversity environments with sources. introducing strategies is necessary reproducing experimental baker's yeast Saccharomyces cerevisiae growing two carbon Even though diauxic shifts emerge naturally model resources qualitatively very different, predicts existence such prerequisite coexistence communities.

Language: Английский

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Nutrient dominance governs the assembly of microbial communities in mixed nutrient environments

eLife, Journal Year: 2021, Volume and Issue: 10

Published: April 20, 2021

A major open question in microbial community ecology is whether we can predict how the components of a diet collectively determine taxonomic composition communities. Motivated by this challenge, investigate communities assembled pairs nutrients be predicted from those every single nutrient alone. We find that although null, naturally additive model generally predicts well family-level composition, there exist systematic deviations predictions reflect generic patterns dominance at family level. Pairs more-similar (e.g. two sugars) are on average more than dissimilar (one sugar–one organic acid). Furthermore, sugar–acid similar to sugar acid community, which may explained asymmetries benefits. Overall, our results suggest regularities interact help responses dietary changes.

Language: Английский

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Microbial community design: methods, applications, and opportunities

Current Opinion in Biotechnology, Journal Year: 2019, Volume and Issue: 58, P. 117 - 128

Published: April 3, 2019

Language: Английский

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Engineering complex communities by directed evolution

Nature Ecology & Evolution, Journal Year: 2021, Volume and Issue: 5(7), P. 1011 - 1023

Published: May 13, 2021

Language: Английский

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