Feeding Drosophila gut microbiomes from young and old flies modifies the microbiome

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 2, 2024

Abstract It is becoming increasingly evident that the myriad of microbes in gut, within cells and attached to body parts (or roots plants), play crucial roles for host. Although this has been known decades, recent developments molecular biology allow expanded insight into abundance function these microbes. Here we used vinegar fly, Drosophila melanogaster , investigate fitness measures across lifetime flies fed a suspension gut harvested from young or old flies, respectively. Our hypothesis was constitutively enriched with ‘Young microbiome’ would live longer be more agile at age (i.e. have increased healthspan) compared an ‘Old microbiome’. Three major take home messages came out our study: (1) microbiomes differ markedly; (2) feeding Young Old altered microbiome recipient (3) two different microbial diets did not any effect on locomotor activity nor lifespan contradicting working hypothesis. Combined, results provide novel interplay between hosts their clearly highlight phenotypic effects transplants probiotics can complex unpredictable.

Language: Английский

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High-fat diets induce inflammatory IMD/NFκB signaling via gut microbiota remodeling in Drosophila

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: April 23, 2024

High-fat diets (HFDs), a prevailing daily dietary style worldwide, induce chronic low-grade inflammation in the central nervous system and peripheral tissues, promoting variety of diseases including pathologies associated with neuroinflammation. However, mechanisms linking HFDs to are not entirely clear. Here, using Drosophila HFD model, we explored mechanism HFD-induced remote tissues. We found that activated IMD/NFκB immune pathway head through remodeling commensal gut bacteria. Removal microbiota abolished such inflammatory response. Further experiments revealed significantly increased abundance Acetobacter malorum gut, re-association this bacterium was sufficient elicit response Mechanistically, produced greater amount peptidoglycan (PGN), well-defined microbial molecular pattern enters circulation remotely activates an Our results thus show trigger mediated by bacterial elicits host

Language: Английский

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Dietary Zinc Limitation Dictates Lifespan and Reproduction Trade‐Offs of Drosophila Mothers

Aging Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

ABSTRACT Dietary metal ions significantly influence the lifespan and reproduction of Drosophila females. In this study, we show that not adding any to diet adversely affects lifespan. By contrast, food with no added Zn negatively impacts but does affect maternal lifespan, indicating it can dictate resource reallocation between key fitness traits. Specifically, indicates female flies stop producing eggs conserve their body for somatic maintenance. Although these data sense varying dietary levels adjust physiology, they cannot maximise egg production when faced a choice or sufficient support maximum reproduction. Nonetheless, choose preferentially oviposit on Zn‐containing food, perhaps strategy assure offspring survival. We also uncovered role white gene in sustaining high viability is diluted diet. These insights into ions, particularly Zn, point central micronutrients indicate environmental quality so govern trade‐offs flies.

Language: Английский

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Different Long-Term Nutritional Regimens of Drosophila melanogaster Shape Its Microbiota and Associated Metabolic Activity in a Sex-Specific Manner

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 141 - 141

Published: Feb. 1, 2025

The dietary habits of fruit flies profoundly influence their fitness, morphology, and physiology yet the mechanisms underlying these effects remain incompletely understood. To address this gap, relationship between regimens composition function adult Drosophila melanogaster microbiota was investigated in present study. fly communities that were reared for long time on five different diets characterized by means 16S rRNA sequencing. Obtained results revealed distinct community structures associated with each regimen, which additionally corroborated through machine learning-based analysis. In general, sugar-rich correlate microbial ecosystems higher richness/diversity. Dominance phyla Proteobacteria Firmicutes confirmed irrespective diet, varying proportions most abundant families: Acetobacteraceae, Lactobacillaceae, Moraxellaceae, Bradyrhizobiaceae, Leucostonocaceae. Bacterial families lower abundance also emerged as differentially among studied groups. Additionally, functional prediction provided initial clues into how nutrient availability might modulate metabolic traits a sex-specific manner to meet host needs. Overall, presented findings highlight intricate interplay composition, phenotype flies, underscoring importance diet determinant host-microbiota interactions.

Language: Английский

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A Drosophila holidic diet optimized for growth and development

Developmental Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Diets composed of chemically pure components (holidic diets) are useful for determining the metabolic roles individual nutrients. For model organism Drosophila melanogaster, existing holidic diets unable to support rapid growth characteristic larval stage. Here, we use a nutrient co-optimization strategy across more than 50 diet variants design fast development (HolFast), medium tailored and development. We identify dietary amino acid ratios optimal developmental speed but show that they compromise survival unless vitamins sterols co-optimized. Rapid on HolFast is not improved by adding fatty acids, it dependent upon their de novo synthesis in fat body via synthase (FASN). outperforms other diets, supporting rates close those yeast-based and, under germ-free conditions, identical. has wide applications nutritional studies

Language: Английский

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