Unraveling the Complexity of Human Brain: Structure, Function in Healthy and Disease States

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 100, P. 102414 - 102414

Published: July 14, 2024

Language: Английский

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Evaluation of the Digital Ventilated Cage® system for circadian phenotyping

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 29, 2025

Language: Английский

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The time is now: accounting for time-of-day effects to improve reproducibility and translation of metabolism research

Nature Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

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Developmental control of rod number via a light-dependent retrograde pathway from intrinsically photosensitive retinal ganglion cells

Developmental Cell, Journal Year: 2024, Volume and Issue: 59(21), P. 2897 - 2911.e6

Published: Aug. 13, 2024

Language: Английский

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Modulation of All-Trans Retinoic Acid by Light and Dopamine in the Murine Eye

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(3), P. 37 - 37

Published: March 18, 2025

Purpose: Ambient light exposure is linked to myopia development in children and affects susceptibility animal models. Currently, it unclear which signals mediate the effects of on myopia. All-trans retinoic acid (atRA) dopamine (DA) oppositely influence experimental may be involved retinoscleral signaling cascade underlying myopic eye growth. However, how ocular atRA responds different lighting whether DA interact remains unknown. Methods: Dark-adapted C57BL/6J mice (29–31 days old) were exposed dim (1 lux), mid (59 or bright (12,000 lux) ambient for 5 60 minutes. Some also systemically administered precursor, LDOPA, before exposure. After exposure, retina back (BOE) collected analyzed levels atRA, DA, metabolite, DOPAC. Results: turnover (DOPAC/DA ratio) increased magnitude after only minutes higher luminance, but was minimal BOE. In contrast, BOE significantly decreased with luminance longer duration Intriguingly, LDOPA-treated had a transient reduction retinal compared saline-treated mice, whereas treatment no effect DA. Conclusions: Ocular affected by lighting, Overall, these data suggest specific interactions between that could have implications

Language: Английский

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Beyond Lux: methods for species and photoreceptor-specific quantification of ambient light for mammals

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 14, 2024

Abstract Background Light is a key environmental regulator of physiology and behaviour. Mistimed or insufficient light disrupts circadian rhythms associated with impaired health well-being across mammals. Appropriate lighting therefore crucial for indoor housed commonly measured in lux. However, this employs spectral weighting function human luminance not suitable ‘non-visual’ effects use species. In humans, photoreceptor-specific (α-opic) metrology system has been proposed as more appropriate way measuring light. Results Here we establish technology to allow α-opic measurement approach be readily extended mammalian species, accounting differences photoreceptor types, photopigment sensitivities, eye anatomy. We develop high-throughput method derive sensitivities recombinantly expressed opsins it the sensitivity melanopsin from 13 non-human further address need simple strategies species-specific measures by developing an accessible online toolbox calculating these units validating open hardware multichannel sensor ‘point click’ measurement. finally demonstrate that measurements are superior photopic lux predictors physiological responses mice ecologically relevant comparisons photosensitivity between Conclusions Our study presents methods existing unit holds promise improvements welfare animals, scientific research reproducibility, agricultural productivity, energy usage.

Language: Английский

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Light sampling behaviour regulates circadian entrainment in mice

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 16, 2024

Abstract Background The natural light environment is far more complex than that experienced by animals under laboratory conditions. As a burrowing species, wild mice are able to self-modulate their exposure, concept known as sampling behaviour. By contrast, conditions have little opportunity exhibit this To address issue, here we introduce simple nestbox paradigm allow environment. Dark nestboxes fitted with passive infrared sensors were used monitor locomotor activity, circadian entrainment, decision making and Results Under these conditions, significantly reduce exposure an average of just 0.8 h across 24 period. In addition, show distinct pattern behaviour, peaks at dawn dusk ramped dark cycle. Furthermore, the timing behaviour depends upon endogenous rhythms abolished in lacking clock, indicating feedback loop between light, clock Conclusions Our results highlight important role modifying signals available for entrainment

Language: Английский

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Practical Advice on Measuring and Applying Light for Laboratory Mammals

Journal of Biological Rhythms, Journal Year: 2024, Volume and Issue: 39(4), P. 323 - 330

Published: July 31, 2024

Light is recognized as an important component of the environment for laboratory animals. It supports vision, sets phase circadian clocks, and drives wide-ranging adjustments in physiological behavioral state. Manipulating light meanwhile a key experimental approach fields vision science chronobiology. Nevertheless, until recently, there has been no consensus on methods quantifying experienced by Widely adopted practices employ metrics such illuminance (units = lux) that are designed to quantify human observers. These weight energy across spectrum according spectral sensitivity profile not widely replicated non-human species. Recently, Consensus View was published proposes measurement standardization take account these species-specific differences wavelength sensitivity. Here, we draw upon contents provide simplified advice mammal experimentation husbandry quantitative guidance what constitutes appropriate lighting both visual function.

Language: Английский

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Photoperiod, food restriction and memory for objects and places in mice

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 16, 2024

Language: Английский

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Methods for applying blinding and randomisation in animal experiments

Laboratory Animals, Journal Year: 2024, Volume and Issue: 58(5), P. 419 - 426

Published: Oct. 1, 2024

Blinding and randomisation are important methods for increasing the robustness of pre-clinical studies, as incomplete or improper implementation thereof is recognised a source bias. Randomisation ensures that any known unknown covariates introducing bias randomly distributed over experimental groups. Thereby, differences between groups might otherwise have contributed to false positive -negative results diminished. Methods range from simple (e.g. rolling dice) advanced strategies involving use specialised software. on other hand researchers unaware group allocation during preparation, execution acquisition and/or analysis data. This minimises risk unintentional influences resulting in blinding require strong protocols team approach. In this review, we outline give practical tips how implement them, with focus animal studies.

Language: Английский

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