Current Biology, Journal Year: 2019, Volume and Issue: 30(1), P. 17 - 30.e3
Published: Dec. 12, 2019
Language: Английский
eLife, Journal Year: 2016, Volume and Issue: 5
Published: Oct. 22, 2016
Internal states can profoundly alter the behavior of animals. A quantitative understanding behavioral changes upon metabolic challenges is key to a mechanistic dissection how animals maintain nutritional homeostasis. We used an automated video tracking setup characterize amino acid and reproductive interact shape exploitation exploration decisions taken by adult Drosophila melanogaster. find that these two have specific effects on stop at leave proteinaceous food patches. Furthermore, internal nutrient state defines exploration-exploitation trade-off: nutrient-deprived flies focus patches while satiated explore more globally. Finally, we show olfaction mediates efficient recognition yeast as appropriate protein source in mated females octopamine specifically required mediate homeostatic postmating responses without affecting sensing. therefore modulate aspects change selection.
Language: Английский
Citations
126
Current Biology, Journal Year: 2017, Volume and Issue: 27(13), P. 1915 - 1927.e5
Published: June 29, 2017
Language: Английский
Citations
113
PLoS ONE, Journal Year: 2015, Volume and Issue: 10(7), P. e0133017 - e0133017
Published: July 13, 2015
The neuroendocrine peptides CCHamide-1 and -2, encoded by the genes ccha1 are produced endocrine cells in midgut neurons brain of Drosophila melanogaster. Here, we used CRISPR/Cas9 technique to disrupt -2 identify mutant phenotypes with a focus on ccha-2 mutants. We found that both larval adult ccha2 mutants showed significantly reduced food intake as measured flies Capillary Feeding (CAFE) assay (up 72% compared wild-type). Locomotion tests had locomotor activity especially around 8 a.m. p.m., where normally feeds 70% Reduced feeding is coupled delayed development, process mediated insulin. Accordingly, remarkably showing pupariation 70 hours after time point wild-type. In contrast, ccha-1 were not developmentally delayed. also up 80% mRNA concentrations coding for insulin-like-peptides-2 -3, while these unchanged From experiments conclude CCHamide-2 an orexigenic peptide important factor controlling developmental timing Drosophila.
Language: Английский
Citations
106
Cell Research, Journal Year: 2018, Volume and Issue: 28(10), P. 1013 - 1025
Published: Sept. 12, 2018
Adequate protein intake is crucial for the survival and well-being of animals. How animals assess prospective sources ensure dietary amino acid plays a critical role in homeostasis. By using quantitative feeding assay, we show that three acids, L-glutamate (L-Glu), L-alanine (L-Ala) L-aspartate (L-Asp), but not their D-enantiomers or other 17 natural L-amino acids combined, rapidly promote food consumption fruit fly Drosophila melanogaster. This feeding-promoting effect independent mating experience internal nutritional status. In vivo ex calcium imagings six brain neurons expressing diuretic hormone 44 (DH44) can be directly activated by these suggesting are an sensor. Genetic inactivation DH44+ abolishes increase induced whereas genetic activation sufficient to feeding, mediate consumption. Single-cell transcriptome analysis immunostaining reveal putative transporter, CG13248, enriched neurons. Knocking down CG13248 expression blocks eliminates responses acids. Therefore, data identify neuron as key sensor detect enhance via transporter CG13248. These results shed light on regulation homeostasis at organismal levels nervous system.
Language: Английский
Citations
105
Scientific Reports, Journal Year: 2018, Volume and Issue: 8(1)
Published: July 26, 2018
Abstract Although the Drosophila melanogaster (fly) model is a popular platform for investigating diet-related phenomena, it can be challenging to measure volume of agar-based food media flies consume. We addressed this challenge by developing dye-based method called Consumption-Excretion (Con-Ex). In Con-Ex studies, consume solid labeled with dye, and consumed reflected sum dye inside excreted flies. Flies consumed-excreted measurable amounts FD&C Blue No. 1 (Blue 1) other dyes in but only was readily detectable at concentrations that had no discernable effect on consumption-excretion. studies 1, consumption-excretion (i) increased linearly feeding duration out 24 h two different laboratory sites, (ii) sensitive starvation, mating status strain, (iii) changed response alteration composition as expected. Additionally, liquid from capillary tubes indistinguishable flies, indicating reflects 1. Our results demonstrate tracer useful assessing ingestion adult
Language: Английский
Citations
104
Nature Metabolism, Journal Year: 2022, Volume and Issue: 4(11), P. 1532 - 1550
Published: Nov. 7, 2022
Abstract Animals must adapt their dietary choices to meet nutritional needs. How these needs are detected and translated into nutrient-specific appetites that drive food-choice behaviours is poorly understood. Here we show enteroendocrine cells of the adult female Drosophila midgut sense nutrients in response release neuropeptide F (NPF), which an ortholog mammalian Y-family gut-brain hormones. Gut-derived NPF acts on glucagon-like adipokinetic hormone (AKH) signalling induce sugar satiety increase consumption protein-rich food, adipose tissue promote storage ingested nutrients. Suppression NPF-mediated gut leads overconsumption while simultaneously decreasing intake yeast. Furthermore, gut-derived has a female-specific function promoting protein-containing food mated females. Together, our findings suggest NPF-to-AKH modulates specific regulates choice ensure homeostatic nutrients, providing insight hormonal mechanisms underlie hungers.
Language: Английский
Citations
61
Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)
Published: Feb. 4, 2022
Abstract The intestine is a central regulator of metabolic homeostasis. Dietary inputs are absorbed through the gut, which senses their nutritional value and relays hormonal information to other organs coordinate systemic energy balance. However, gut-derived hormones affecting behavioral responses poorly defined. Here we show that endocrine cells Drosophila gut sense nutrient stress mechanism involves TOR pathway in response secrete peptide hormone allatostatin C, somatostatin homolog. Gut-derived C induces secretion glucagon-like adipokinetic food intake mobilization. Loss Allatostatin or its receptor adipokinetic-hormone-producing impairs lipid sugar mobilization during fasting, leading hypoglycemia. Our findings illustrate nutrient-responsive maintains homeostasis under nutrient-stress conditions, function essential health whose failure can lead disorders.
Language: Английский
Citations
57
Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)
Published: June 7, 2022
Abstract An organism’s ability to perceive and respond changes in its environment is crucial for health survival. Here we reveal how the most well-studied longevity intervention, dietary restriction, acts in-part through a cell non-autonomous signaling pathway that inhibited by presence of attractive smells. Using an intestinal reporter key gene induced restriction but suppressed smells, identify three compounds block food odor effects C. elegans , thereby increasing as mimetics. These clearly implicate serotonin dopamine limiting lifespan response odor. We further chemosensory neuron likely perceives odor, enteric signals receptor 5-HT1A/SER-4, dopaminergic DRD2/DOP-3. Aspects this are conserved D. melanogaster . Thus, blocking antagonism or receptors plausible approach mimic benefits restriction.
Language: Английский
Citations
44
Science, Journal Year: 2023, Volume and Issue: 380(6645), P. 625 - 632
Published: May 11, 2023
Hunger is an ancient drive, yet the molecular nature of pressures this sort and how they modulate physiology are unknown. We find that hunger modulates aging in Drosophila. Limitation branched-chain amino acids (BCAAs) or activation hunger-promoting neurons induced a state extended life span despite increased feeding. Alteration neuronal histone acetylome was associated with BCAA limitation, preventing these alterations abrogated effect limitation to increase feeding extend span. acutely through usage variant H3.3, whereas prolonged seemed decrease set point, resulting beneficial consequences for aging. Demonstration sufficiency reveals motivational states alone can be deterministic drivers
Language: Английский
Citations
31
Current Protocols, Journal Year: 2023, Volume and Issue: 3(8)
Published: Aug. 1, 2023
The use of Drosophila melanogaster for studies toxicology has grown considerably in the last decade. model long been appreciated as a versatile and powerful developmental biology genetics because its ease handling, short life cycle, low cost maintenance, molecular genetic accessibility, availability wide range publicly available strains data resources. These features, together with recent unique developments genomics metabolomics, make fly especially relevant timely development new approach methodologies movements toward precision toxicology. Here, we offer perspective on how flies can be leveraged to identify risk factors environmental exposures human health. First, review discuss fundamental toxicologic principles experimental design Drosophila. Next, describe quantitative systems approaches resolve architecture candidate pathways controlling susceptibility toxicants. Finally, summarize current state future promise emerging field metabolomics elaborating toxic mechanisms. © 2023 Authors. Current Protocols published by Wiley Periodicals LLC.
Language: Английский
Citations
25