Gut bacteria of the silkworm Bombyx mori facilitate host resistance against the toxic effects of organophosphate insecticides

Environment International, Journal Year: 2020, Volume and Issue: 143, P. 105886 - 105886

Published: July 2, 2020

Organophosphate insecticides that are heavily used in agriculture for pest control have caused growing environmental problems and public health concerns worldwide. Ironically, insecticide resistance develops quickly major lepidopteran pests, partially via their microbial symbionts. To investigate the possible mechanisms by which microbiota confers to Lepidoptera, model organism silkworm Bombyx mori (Lepidoptera: Bombycidae) was fed different antibiotics induce gut dysbiosis (microbiota imbalance). Larvae treated with polymyxin showed a significantly lower survival rate when exposed chlorpyrifos. Through high-throughput sequencing, we found abundances of Stenotrophomonas Enterococcus spp. changed substantially after treatment. assess roles played these two groups bacteria chlorpyrifos resistance, germ-free (GF) rearing protocol established avoid influence natural antibiotics. Monoassociation GF silkworms enhanced host chlorpyrifos, but not Enterococcus-fed larvae, consistent larval detoxification activity. GC-μECD detection residues feces indicated neither nor degraded directly gut. However, metabolomics analysis revealed highly species-specific pattern, higher levels essential amino acid produced larvae monoassociated Stenotrophomonas. This critical nutrient provisioning increased fitness thereby allowed circumvent deleterious effects toxic chemicals more efficiently. Altogether, our study only suggests new mechanism notorious pests also provides useful template investigating interplay between complex systems.

Language: Английский

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Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors

Scientific Reports, Journal Year: 2018, Volume and Issue: 8(1)

Published: Jan. 26, 2018

Abstract In light of the declining global malaria burden attained largely due to insecticides, a deeper understanding factors driving insecticide resistance is needed mitigate its growing threat vector control programs. Following evidence microbiota-mediated in agricultural pests, we undertook comparative study microbiota mosquitoes differing status. The wild-caught Anopheles albimanus , an important Latin American vector, that were resistant (FEN_Res) or susceptible (FEN_Sus) organophosphate (OP) fenitrothion characterized and compared using whole metagenome sequencing. Results showed composition functions between FEN_Res FEN_Sus, with significant enrichment OP-degrading bacteria enzymes FEN_Sus. Lower bacterial diversity was observed suggesting taxa competitive advantage response selection pressure. We report characterize for first time metagenomes An. revealing associations phenotypic fenitrothion. This lays groundwork further investigation role mosquito resistance.

Language: Английский

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Gut bacteria of field-collected larvae of Spodoptera frugiperda undergo selection and are more diverse and active in metabolizing multiple insecticides than laboratory-selected resistant strains

Journal of Pest Science, Journal Year: 2020, Volume and Issue: 93(2), P. 833 - 851

Published: Jan. 30, 2020

Language: Английский

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The Honeybee Gut Microbiota Is Altered after Chronic Exposure to Different Families of Insecticides and Infection by <i>Nosema ceranae</i>

Microbes and Environments, Journal Year: 2019, Volume and Issue: 34(3), P. 226 - 233

Published: Jan. 1, 2019

The gut of the European honeybee Apis mellifera is site exposure to multiple stressors, such as pathogens and ingested chemicals. Therefore, microbiota, which contributes host homeostasis, may be altered by these stressors. abundance major bacterial taxa in was evaluated response infection with intestinal parasite Nosema ceranae or chronic low doses neurotoxic insecticides coumaphos, fipronil, thiamethoxam, imidacloprid. Experiments were performed under laboratory conditions on adult workers collected from hives February (winter bees) July (summer revealed season-dependent changes community composition. N. a lethal fipronil treatment increased relative both Gilliamella apicola Snodgrassella alvi surviving winter honeybees. sublethal all decreased Bifidobacterium spp. Lactobacillus regardless season. similar effects induced belonging distinct molecular families suggested shared indirect mode action possibly through aspecific alterations homeostasis. These results demonstrate that concentrations affect holobiont.

Language: Английский

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What We Don't Know About Diet-Breadth Evolution in Herbivorous Insects

Annual Review of Ecology Evolution and Systematics, Journal Year: 2020, Volume and Issue: 51(1), P. 103 - 122

Published: July 31, 2020

Half a million species of herbivorous insects have been described. Most them are diet specialists, using only few plant as hosts. Biologists suspect that their specificity is key to diversity. But why do tend be specialists? In this review, we catalog broad range explanations. We review the evidence for each and suggest lines research obtain lack. then draw attention second major question, namely how changes in breadth affect rest species’ biology. particular, know little about feed back on genetic architecture, population environment, other aspects ecology. Knowing more generalists specialists differ should go long way toward sorting out potential explanations specificity, yield deeper understanding insect

Language: Английский

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Pesticide resistance in arthropods: Ecology matters too

Ecology Letters, Journal Year: 2022, Volume and Issue: 25(8), P. 1746 - 1759

Published: June 21, 2022

Pesticide resistance development is an example of rapid contemporary evolution that poses immense challenges for agriculture. It typically evolves due to the strong directional selection pesticide treatments exert on herbivorous arthropods. However, recent research suggests some species are more prone evolve than others their evolutionary history and standing genetic variation. Generalist might develop especially rapidly pre-adaptation handle a wide array plant allelochemicals. Moreover, has shown adaptation novel host plants could lead increased resistance. Exploring such cross-resistance between range from ecological perspective needed understand its causes consequences better. Much has, however, been devoted molecular mechanisms underlying while both contexts facilitate have under-studied. Here, we take eco-evolutionary approach discuss circumstances may in arthropods plant-arthropod interactions target non-target interactions. Furthermore, suggest future avenues practical implications understanding evolution.

Language: Английский

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Role of gut symbionts of insect pests: A novel target for insect-pest control

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: March 13, 2023

Insects possess beneficial and nuisance values in the context of agricultural sector human life around them. An ensemble gut symbionts assists insects to adapt diverse extreme environments occupy every available niche on earth. Microbial symbiosis helps host by supplementing necessary diet elements, providing protection from predators parasitoids through camouflage, modulation signaling pathway attain homeostasis trigger immunity against pathogens, hijacking plant pathways circumvent defence, acquiring capability degrade chemical pesticides, degradation harmful pesticides. Therefore, a microbial strategy can lead overpopulation insect pests, which drastically reduce crop yield. Some studies have demonstrated increased mortality via destruction symbionts; use antibiotics. The review summarizes various roles played microbiota pests some that been conducted pest control targeting symbionts. Manipulation or exploitation alters growth population is consequently potential target for development better strategies. Methods such as CRISPR/Cas9, RNAi combining IIT SIT increase are further discussed. In ongoing management scenario, proving be reliable, eco-friendly novel approach integrated management.

Language: Английский

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Impact of intraspecific variation in insect microbiomes on host phenotype and evolution

The ISME Journal, Journal Year: 2023, Volume and Issue: 17(11), P. 1798 - 1807

Published: Sept. 2, 2023

Abstract Microbes can be an important source of phenotypic plasticity in insects. Insect physiology, behaviour, and ecology are influenced by individual variation the microbial communities held within insect gut, reproductive organs, bacteriome, other tissues. It is becoming increasingly clear how microbiome for fitness, expansion into novel ecological niches, environments. These investigations have garnered heightened interest recently, yet a comprehensive understanding intraspecific assembly function these insect-associated shape insects still lacking. Most research focuses on core associated with species ignores variation. We argue that among driver evolution, we provide examples showing such influence fitness health insects, invasions, their persistence new environments, responses to global environmental changes.

Language: Английский

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Insect Microbial Symbionts: Ecology, Interactions, and Biological Significance

Microorganisms, Journal Year: 2023, Volume and Issue: 11(11), P. 2665 - 2665

Published: Oct. 30, 2023

The guts of insect pests are typical habitats for microbial colonization and the presence bacterial species inside gut confers several potential advantages to insects. These bacteria located symbiotically digestive tracts insects help in food digestion, phytotoxin breakdown, pesticide detoxification. Different shapes chemical assets gastrointestinal have a significant impact on structure makeup population. number communities system differs owing varying shape composition tracts. Due their short generation times rapid evolutionary rates, can develop numerous metabolic pathways adapt diverse ecological niches. In addition, despite hindering insecticide management programs, they still biotechnological uses, including industrial, clinical, environmental uses. This review discusses prevalent associated with guts, mode symbiotic interaction, role resistance, various other biological significance, along knowledge gaps future perspectives. practical consequences microbiome its interaction host may lead encountering mechanisms behind evolution resistance

Language: Английский

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The role of microbiomes in shaping insecticide resistance: current insights and emerging paradigms

Current Opinion in Insect Science, Journal Year: 2025, Volume and Issue: unknown, P. 101346 - 101346

Published: Feb. 1, 2025

Language: Английский

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