Characterization of acute pain‐induced behavioral passivity in mice: Insights from statistical modeling DOI
Sung Ching Yeung, Kumar Ganesan,

Stanley Sau Ching Wong

et al.

European Journal of Neuroscience, Journal Year: 2021, Volume and Issue: 53(9), P. 3072 - 3092

Published: March 6, 2021

Abstract Affective‐motivational disturbances are highly inconsistent in animal pain models. The reproducibility of the open‐field test assessing anxiety, malaise or disability remains controversial despite its popularity. While traumatic, persistent multiregional models commonly considered more effective inducing negative affect functional impairment, early psychobehavioral changes before chronification often underexplored. Here, we aimed to clarify fundamental relationship between hypernociception and passive distress‐like behavior using a model transient inflammatory pain. To minimize latent confounders increase data consistency, male C57BL/6N mice were habituated arena 6 times receiving unilateral intraplantar injection prostaglandin E2 (PGE2) vehicle. Open‐field (40‐min exploration) nociceptive evaluated repeatedly along course both wild‐type transgenic with known pronociceptive phenotype. reduce subjectivity, multivariate behavioral outcomes analyzed by statistical modeling based on exploratory factor analyses, which yielded 2‐factor solution. Within 3 hr after PGE2 injection, developed significantly reduced center exploration (factor 1) marginally significant their habituation tendency 2), not apparent vehicle‐injected mice. passivity generally improved as subsided. Therefore, irritation is sufficient suppress mouse activity. absence late affective‐motivational some rodents prolonged may imply lack preceding underlying neuropsychological alterations. Procedural invasive experiments, however small, should be assessed adequately controlled potential research confounder.

Language: Английский

Measuring Anxiety-Like Behaviors in Rodent Models of Traumatic Brain Injury DOI Creative Commons
Laura B. Tucker, Joseph T. McCabe

Frontiers in Behavioral Neuroscience, Journal Year: 2021, Volume and Issue: 15

Published: Oct. 29, 2021

Anxiety is a common complaint following acquired traumatic brain injury (TBI). However, the measurement of dysfunctional anxiety behavioral states experimental TBI in rodents complex. Some studies report increased after TBI, whereas others find decreased anxiety-like state, often described as risk-taking behavior or impulsivity. These inconsistencies may reflect lack standardization models testing techniques. Here, we review most commonly employed unconditioned tests and discuss them context TBI. Special attention given to effects repeated testing, consideration potential sensory motor confounds injured rodents. The use multiple alternative data analysis methods are discussed, well for application elements (CDEs) means providing format documentation details procedures each published research report. CDEs improve rigor, reproducibility, endpoint better relating findings with clinical phenotypes final goal translation. While this not resolve all incongruities across laboratories, it seen way forward standardized universal collection improvement quality sharing, advance therapies neuropsychiatric symptoms that present decades

Language: Английский

Citations

42
Deep phenotyping reveals movement phenotypes in mouse neurodevelopmental models DOI Creative Commons
Ugne Klibaite, Mikhail Kislin, Jessica L. Verpeut

et al.

Molecular Autism, Journal Year: 2022, Volume and Issue: 13(1)

Published: March 12, 2022

Repetitive action, resistance to environmental change and fine motor disruptions are hallmarks of autism spectrum disorder (ASD) other neurodevelopmental disorders, vary considerably from individual individual. In animal models, conventional behavioral phenotyping captures such fine-scale variations incompletely. Here we observed male female C57BL/6J mice methodically catalog adaptive movement over multiple days examined two rodent models developmental disorders against this dynamic baseline. We then investigated the consequences a cerebellum-specific deletion in Tsc1 protein whole-brain knockout Cntnap2 mice. Both these mutations found clinical conditions have been associated with ASD.We used advances computer vision deep learning, namely generalized form high-dimensional statistical analysis, develop framework for characterizing mouse on timescales using single popular assay, open-field test. The pipeline takes virtual markers pose estimation find behavior clusters generate wavelet signatures classes. measured spatial temporal habituation new environment across minutes days, different types self-grooming, locomotion gait.Both knockouts L7-Tsc1 mutants showed forelimb lag during gait. complex defects multi-day adaptation, lacking tendency wild-type spend progressively more time corners arena. mutant mice, failure adapt took maintained ambling, turning locomotion, an overall decrease grooming. However, adaptation traits was similar between knockouts. patterns state occupancy.Genetic risk factors numerous, tested only two. Our done under free behavior. Testing task or social would reveal information about dynamics variability.Our automated successfully model-specific deviations as well differences detailed structure dynamics. reported deficits indicate that constitutes robust set ASD symptoms may be considered implementation settings quantitative diagnosis criteria.

Language: Английский

Citations

33
A systematic review of the development and application of home cage monitoring in laboratory mice and rats DOI Creative Commons
Pia Kahnau, Paul Mieske, Jenny Wilzopolski

et al.

BMC Biology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Nov. 13, 2023

Abstract Background Traditionally, in biomedical animal research, laboratory rodents are individually examined test apparatuses outside of their home cages at selected time points. However, the outcome such tests can be influenced by various factors and valuable information may missed when animals only monitored for short periods. These issues overcome longitudinally monitoring mice rats cages. To shed light on development cage (HCM) current state-of-the-art, a systematic review was carried out 521 publications retrieved through PubMed Web Science. Results Both absolute (~ × 26) relative 7) number HCM-related increased from 1974 to 2020. There clear bias towards males housed animals, but during past decade (2011–2020), an increasing studies used both sexes group housing. In most studies, were kept (up 4 weeks) periods HCM systems; intermediate (4–12 frequency years between 2011 Before 2000s, techniques predominantly applied less than 12 h, while 24-h measurements have been more frequent since 2000s. The demonstrated that manual is decreasing relation automatic still relevant. Until (and including) 1990s, manually progressively replaced automation Independent year publication, main behavioral parameters measured locomotor activity, feeding, social behaviors; physiological heart rate electrocardiography. External appearance-related rarely Due technological progress application artificial intelligence, refined detailed investigated recently. Conclusions Over period covered this study, improved considerably. This ongoing further as well validation systems will extend applications allow continuous, longitudinal, non-invasive range group-housed small

Language: Английский

Citations

19
Reporting checklists in neuroimaging: promoting transparency, replicability, and reproducibility DOI
Hamed Ekhtiari, Mehran Zare-Bidoky, Arshiya Sangchooli

et al.

Neuropsychopharmacology, Journal Year: 2024, Volume and Issue: 50(1), P. 67 - 84

Published: Sept. 6, 2024

Language: Английский

Citations

4
Transient cortical beta-frequency oscillations associated with contextual novelty in high density mouse EEG DOI Creative Commons
Callum Walsh, Luke Tait, Maria Garcia Garrido

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 23, 2025

Language: Английский

Citations

0
The Presence of a Shelter in an Open Field Test Has Differential Effects on the Behavior and Stress Response of Two Mouse Species DOI Creative Commons
Rita I. Monarca, Ricardo Silva, Sofia I. Gabriel

et al.

Journal of Experimental Zoology Part A Ecological and Integrative Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

ABSTRACT The open field test (OFT) is frequently used in research to assess anxiety‐like behavior and locomotor activity. Its simple design can lead the misconception that it a standardized procedure comparable between laboratories. However, some modifications setup cause changes behavior. Different species might also react differently introduced. There thus need for better understanding of impact their value question. Here, we tested two closely related mouse species, Mus musculus spretus , an OFT with without presence shelter. We assessed exploratory through analysis multiple behavioral traits, stress response measurement circulating cortisol levels. Both had elevated levels during contrast control animals which were not exposed OFT. While shelter increased both M. but showed reduction Also, other measured behaviors show rather proactive coping strategy commensal reactive non‐commensal . Our study revealed strong species‐specific influence on resulting mice, illustrating importance designs account characteristics under study. addition be considered improve experimental results by promoting animal welfare.

Language: Английский

Citations

0
Temporal consistency and associations between behavioural responses of foragers of an Ectatominae ant in a multiple test set-up DOI
Veridiana Jardim, Ronara Souza Ferreira,

Elvio Nunes

et al.

Insectes Sociaux, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Language: Английский

Citations

0
Ensuring Integrity in AI Research: Frameworks for Assessing Validity and Reliability of GenAI Outputs DOI

M Poornesh

The Clearing House A Journal of Educational Strategies Issues and Ideas, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13

Published: April 27, 2025

Language: Английский

Citations

0
The light-dark forced swim test for simultaneous assessment of behavioral ‘despair’ and anxiety-like behavior in female mice DOI
Sergey L. Khatsko, Alexander V. Zhdanov,

Daria V Kravchenko

et al.

Behavioural Brain Research, Journal Year: 2025, Volume and Issue: 484, P. 115492 - 115492

Published: Feb. 20, 2025

Language: Английский

Citations

0
Blueprints for measuring natural behavior DOI Creative Commons
Alicja Puścian, Ewelina Knapska

iScience, Journal Year: 2022, Volume and Issue: 25(7), P. 104635 - 104635

Published: June 18, 2022

Until recently laboratory tasks for studying behavior were highly artificial, simplified, and designed without consideration the environmental or social context. Although such an approach offers good control over behavior, it does not allow researching either voluntary responses individual differences. Importantly neuroscience studies, activity of neural circuits involved in producing unnatural, artificial is variable hard to predict. In addition, different ensembles may be activated depending on strategy animal adopts deal with spurious problem. Thus, simplified based responses, which do occur spontaneously entail problems modeling behavioral impairments underlying brain deficits. To develop valid models human disorders we need test spontaneous behaviors consistently engaging well-defined, evolutionarily conserved neuronal circuits. Such research focuses patterns relevant surviving thriving under varying conditions, also enable high reproducibility across testing settings.

Language: Английский

Citations

16