Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain DOI Creative Commons
Gabriella R Sterne, Hideo Otsuna, Barry J. Dickson

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Sept. 2, 2021

Neural circuits carry out complex computations that allow animals to evaluate food, select mates, move toward attractive stimuli, and away from threats. In insects, the subesophageal zone (SEZ) is a brain region receives gustatory, pheromonal, mechanosensory inputs contributes control of diverse behaviors, including feeding, grooming, locomotion. Despite its importance in sensorimotor transformations, study SEZ has been hindered by limited knowledge underlying diversity neurons. Here, we generate collection split-GAL4 lines provides precise genetic targeting 138 different cell types adult Drosophila melanogaster, comprising approximately one third all We characterize single-cell anatomy these neurons find they cluster morphology into six supergroups organize discrete anatomical domains. majority local interneurons are not classically polarized, suggesting rich processing, whereas projection tend be conveying information number higher regions. This insight organization generates resources will facilitate further their contributions sensory processing behavior.

Language: Английский

A functionally ordered visual feature map in the Drosophila brain DOI Creative Commons
Nathan C Klapoetke, Aljoscha Nern, Edward M. Rogers

et al.

Neuron, Journal Year: 2022, Volume and Issue: 110(10), P. 1700 - 1711.e6

Published: March 14, 2022

Topographic maps, the systematic spatial ordering of neurons by response tuning, are common across species. In Drosophila, lobula columnar (LC) neuron types project from optic lobe to central brain, where each forms a glomerulus in distinct position. However, advantages this glomerular arrangement unclear. Here, we examine functional and relationships 10 glomeruli using single-neuron calcium imaging. We discover novel detectors for objects smaller than lens resolution (LC18) complex line motion (LC25). find that spatially clustered selectivity looming versus drifting object ordered size tuning form topographic visual feature map. Furthermore, connectome analysis shows downstream integrate sparse subsets possible combinations, which biased encoding similar features. LC thus an explicit example topographically organized facilitate circuit integration.

Language: Английский

Citations

78
Ascending neurons convey behavioral state to integrative sensory and action selection brain regions DOI Creative Commons
Chin‐Lin Chen, Florian Aymanns, Ryo Minegishi

et al.

Nature Neuroscience, Journal Year: 2023, Volume and Issue: 26(4), P. 682 - 695

Published: March 23, 2023

Knowing one's own behavioral state has long been theorized as critical for contextualizing dynamic sensory cues and identifying appropriate future behaviors. Ascending neurons (ANs) in the motor system that project to brain are well positioned provide such signals. However, what ANs encode where they convey these signals remains largely unknown. Here, through large-scale functional imaging behaving animals morphological quantification, we report encoding targeting of hundreds genetically identifiable adult fly, Drosophila melanogaster. We reveal states, specifically conveying self-motion anterior ventrolateral protocerebrum, an integrative hub, discrete actions gnathal ganglia, a locus action selection. Additionally, AN projection patterns within predictive their encoding. Thus, ascending populations poised inform distinct hubs ongoing behaviors may important substrate computations required adaptive behavior.

Language: Английский

Citations

48
A Connectome of the MaleDrosophilaVentral Nerve Cord DOI Creative Commons
Shin-ya Takemura, Kenneth J. Hayworth, Gary B. Huang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 6, 2023

Abstract Animal behavior is principally expressed through neural control of muscles. Therefore understanding how the brain controls requires mapping neuronal circuits all way to motor neurons. We have previously established technology collect large-volume electron microscopy data sets tissue and fully reconstruct morphology neurons their chemical synaptic connections throughout volume. Using these tools we generated a dense wiring diagram, or connectome, for large portion Drosophila central brain. However, in most animals, including fly, majority are located outside center closer body, i.e. mammalian spinal cord insect ventral nerve (VNC). In this paper, extend our effort map full by generating connectome VNC male fly.

Language: Английский

Citations

48
Generating parallel representations of position and identity in the olfactory system DOI Creative Commons
István Taisz, Erika Donà, Daniel Münch

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(12), P. 2556 - 2573.e22

Published: May 25, 2023

In Drosophila, a dedicated olfactory channel senses male pheromone, cis-vaccenyl acetate (cVA), promoting female courtship while repelling males. Here, we show that separate cVA-processing streams extract qualitative and positional information. cVA sensory neurons respond to concentration differences in 5-mm range around male. Second-order projection encode the angular position of by detecting inter-antennal concentration, which are amplified through contralateral inhibition. At third circuit layer, identify 47 cell types with diverse input-output connectivity. One population responds tonically flies, second is tuned looming, integrates taste coincidentally promote mating. The separation features resembles mammalian what where visual streams; together multisensory integration, this enables behavioral responses appropriate specific ethological contexts.

Language: Английский

Citations

45
NeuronBridge: an intuitive web application for neuronal morphology search across large data sets DOI Creative Commons
Jody Clements, Cristian Goina, Philip M. Hubbard

et al.

BMC Bioinformatics, Journal Year: 2024, Volume and Issue: 25(1)

Published: March 15, 2024

Abstract Background Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. To exploit this knowledge base, researchers relate connectome’s structure neuronal function, often by studying individual neuron cell types. Vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM), enabling targeting for experimentation. However, creating line driving gene expression within single found an EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Results Here, we present NeuronBridge, web application easily rapidly putative morphological matches between We describe functionality construction NeuronBridge service, including its user-friendly graphical user interface (GUI), extensible model, serverless cloud architecture, massively parallel image search engine. Conclusions fills critical gap workflow used hundreds neuroscience around world. offer our software code, open APIs, processed integration reuse, provide service at http://neuronbridge.janelia.org .

Language: Английский

Citations

29
Mapping the neural dynamics of locomotion across the Drosophila brain DOI

Bella E. Brezovec,

Andrew B. Berger,

Yukun A. Hao

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(4), P. 710 - 726.e4

Published: Jan. 21, 2024

Language: Английский

Citations

28
Connectome-driven neural inventory of a complete visual system DOI Creative Commons
Aljoscha Nern, Frank Loesche, Shin-ya Takemura

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 18, 2024

Vision provides animals with detailed information about their surroundings, conveying diverse features such as color, form, and movement across the visual scene. Computing these parallel spatial requires a large network of neurons, that in distant flies humans, regions comprise half brain's volume. These brain often reveal remarkable structure-function relationships, neurons organized along maps shapes directly relate to roles processing. To unravel stunning diversity complex system, careful mapping neural architecture matched tools for targeted exploration circuitry is essential. Here, we report new connectome right optic lobe from male Drosophila central nervous system FIB-SEM volume comprehensive inventory fly's neurons. We developed computational framework quantify anatomy establishing basis interpreting how vision. By integrating this analysis connectivity information, neurotransmitter identity, expert curation, classified ~53,000 into 727 types, which are systematically described named first time. Finally, share an extensive collection split-GAL4 lines our neuron type catalog. Together, set data unlock possibilities systematic investigations vision Drosophila, foundation deeper understanding sensory

Language: Английский

Citations

28
A Connectome of the Male Drosophila Ventral Nerve Cord DOI Open Access
Shin-ya Takemura, Kenneth J. Hayworth, Gary B. Huang

et al.

Published: May 23, 2024

Animal behavior is principally expressed through neural control of muscles. Therefore understanding how the brain controls requires mapping neuronal circuits all way to motor neurons. We have previously established technology collect large-volume electron microscopy data sets tissue and fully reconstruct morphology neurons their chemical synaptic connections throughout volume. Using these tools we generated a dense wiring diagram, or connectome, for large portion Drosophila central brain. However, in most animals, including fly, majority are located outside center closer body, i.e. mammalian spinal cord insect ventral nerve (VNC). In this paper, extend our effort map full by generating connectome VNC male fly.

Language: Английский

Citations

26
Nested neural circuits generate distinct acoustic signals during Drosophila courtship DOI Creative Commons
Joshua L. Lillvis, Kaiyu Wang, Hiroshi Shiozaki

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(4), P. 808 - 824.e6

Published: Jan. 30, 2024

Many motor control systems generate multiple movements using a common set of muscles. How are premotor circuits able to flexibly diverse movement patterns? Here, we characterize the neuronal that drive distinct courtship songs Drosophila melanogaster. Male flies vibrate their wings toward females produce two different song modes—pulse and sine song—which signal species identity male quality. Using cell-type-specific genetic reagents connectome, provide cellular synaptic map in ventral nerve cord these examine how activating or inhibiting each cell type within affects song. Our data reveal circuit is organized into nested feedforward pathways with extensive reciprocal feedback connections. The larger network produces pulse song, more complex ancestral form. A subset this simpler recent Such organization may be feature which evolution has layered increasing flexibility onto basic pattern.

Language: Английский

Citations

24
Motor neurons generate pose-targeted movements via proprioceptive sculpting DOI
Benjamin Gorko, Igor Siwanowicz,

Kari Close

et al.

Nature, Journal Year: 2024, Volume and Issue: 628(8008), P. 596 - 603

Published: March 20, 2024

Language: Английский

Citations

21